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이준범,Lee Byoung-hee,박정준,Hong Jeong In,Lee Dae-Weon 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.3
The tobacco whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae), is a serious pest that transmits tomato yellow leaf curl virus (TYLCV) in tomato. The tobacco whitefly exhibits morphological similarity with the greenhouse whitefly, Trialeurodes vaporariorum (Hemiptera: Aleyrodidae) which also damages various plants in the green house. Therefore, it is very important to quickly and accurately diagnose whiteflies for applying pest manage ment strategies. In this study, we used a loop-mediated isothermal amplification (LAMP) assay to quickly and effectively detect B. tabaci. Primer sets were investigated for the specificity of B. tabaci by visible detection of the target DNA fragment. Two primer sets for pheromone biosynthesis activating neuropeptide (PBAN) gene were obtained from transcriptomic analysis of B. tabaci biotype Q. The PBAN-based LAMP primer set showed specific amplification of the target region. The optimal conditions for B. tabaci detection were 60 ◦ C for 60 min with four LAMP primers of PBAN. The minimum amount of genomic DNA required for visible detection was 100 ng. These results suggest that the PBAN-based LAMP assay can be applicable for field monitoring of B. tabaci.
1D 전산해석코드를 활용한 차량 엔진 냉각모듈의 성능 예측 및 최적화
이승호(S. H. Lee),김학준(H. J. Kim),차용길(Y. K. Cha),김광일(K. I. Kim) 한국전산유체공학회 2014 한국전산유체공학회 학술대회논문집 Vol.2014 No.5
The performance of engine cooling system is influenced by various factors which are bumper, opening area of grill, radiator, condenser, cooling fan etc. To optimize the engine cooling system is important as well as improvement of component of engine cooling module. To predict performance of engine cooling system using the 1D simulation code is very helpful at early development stage because the simulation can reduce the time and cost of test. This study focuses on prediction of performance of engine cooling system using the 1D simulation code and optimization of engine cooling module using the Taguchi method.