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Rhodocyclus gelatinosus KUP - 74 의 고정화균체로부터 δ - Aminolevulinate 의 연속생산
이소희(So He Lee),김현호(Hyun Ho Kim),윤순규(Sun Kyu Yun),임왕진(Wang Jin Lim),황세영(Se Young Hwang) 한국응용생명화학회 1995 Applied Biological Chemistry (Appl Biol Chem) Vol.38 No.1
An optimal condition for the continuous production of δ-aminolevulinate(ALA) was investigated using high concentrated resting cells of Rhodocyclus gelatinosus KUP-74. The increase of the amount of extracellular ALA versus the concentration of resting cells showed rectangular hyperbolic pattern until 20 ㎎ cells/㎖, but no further increase in the ALA amount by increasing its concentration was occurred. The highest yield of the extracellular ALA was observed after 3 hr of incubation of 1 ㎖ reaction system containing 20 ㎎ cells, 4 mM lewlinate and 5 mM L-glutamate. On the other hand, the immobilized cells prepared by Ca-alginate inclusive method needed to incubate for 6 hr with 6 mM levulinate and 10 mM L-glutamate to give maximal yield of the extracellular ALA. In addition, under these conditions the resulted continuous productivities of the ALA by immobilized cells and highly concentrated resting cells were appeared 50 percent decreases after incubations for 185 hr and 100 hr, respectively, and the method of the cells to be immobilized was more efficient to recover the extracellular ALA produced.
임경환 ( Kyung Hwan Lim ),강민구 ( Min Koo Kang ),김병근 ( Byung Keun Kim ),김민혜 ( Min Hye Kim ),박한기 ( Han Ki Park ),임정아 ( Jung Ah Lim ),권재우 ( Jae Woo Kwon ),김태완 ( Tae Wan Kim ),정재우 ( Jae Woo Jung ),이소희 ( So He 대한천식알레르기학회 2010 천식 및 알레르기 Vol.30 No.2
A 58-year-old man experienced generalized urticaria, orbital angioedema and dyspnea with ingestion of raw ginseng. The prick to prick skin test with raw ginseng showed a strong positive response and oral provocation with unprocessed raw freshginseng provoked facial flushing, generalized urticaria, dyspnea and bronchospasm, which were recovered by epinephrine injection. In the ELISA inhibition test, IgE binding to freshginseng was significantly inhibited by the fresh extracts of ginseng and birch. SDS-PAGE and immunoblot assays revealed an IgE-binding component, and common protein band was detected at 17 kDa (fresh ginseng-birch). This is the first case of freshginseng induced anaphylaxis, and our results suggest that freshginseng anaphylaxis is induced via an IgE-mediated mechanism and may have cross-reactivity with birch. (Korean J Asthma Allergy Clin Immunol 2010;30:131-134)