십이지장내 지방투여로 인한 담낭수축과 혈청 Cholecystokinin의 변화 및 Atropine의 효과

이상인(Sang In Lee),윌리암(William Y . Chey) 대한소화기학회 1994 대한소화기학회지 Vol.26 No.4

N/A The aim of this study is to determine if fat in the duodenum affects gallbladder contraction and plasma cholecystokinin. We evaluated the effect of intraduodenal(I.d.) lipomul on plasma concentration of cholecystokinin-like immunoreactivity(CCK-LI) and gallbladder(GB) contrac- tion in 8 human volunteers. In addition, effects of atropine on plasrna CCK-LI and GB contrac- tion were studied. GB contraction was calculated by measuring GB volume by serial ultrasonograms and expressed as % of the basal volume. Whereas iv saline failed to influence either plasma CCK-LI or GB volume, lipomul in 3 different doses(0.25, 0.5, 1.0ml/kg) pro- duced significant increases(P<0.05) in plasma CCK-LI and significant decreases(P<0.05) in GB volume during a 90 min period of observation. However, the responses of CCK-LI and GB volume were not dose related. Atropine sulfate given iv, 15ug/kg/hr, inhibited lipomul induced GB contraction during the first 15 min which was followed by a partial contraction(not signif- icant). No significant effect of atropine on plasma CCK-LI was apparent also. Thus we con- clude that lipomul produced significant increases in contraction of GB and plasma CCK-LI. The peak concentration of CCK-LI coincided with the rnaximum GB contraction. Atropine did not significantly influence lipomul-stimulated GB contraction and did not influence plasma CCK-LI. Thus no significant cholinergic effect was apparent. (Korean J Gastroenterol 1994; 26: 675 682)

천연물 / 유기화학 : 고지방식이로 유도한 비만 흰쥐에서 Monascus pilosus 균사체 추출물의 간 손상 예방효과

이상일 ( Sang Il Lee ),김재원 ( Jae Won Kim ),이예경 ( Ye Kyung Lee ),양승환 ( Seung Hwan Yang ),이인애 ( In Ae Lee ),서주원 ( Joo Won Suh ),김순동 ( Soon Dong Kim ) 한국응용생명화학회(구 한국농화학회) 2011 Journal of Applied Biological Chemistry (J. Appl. Vol.54 No.3

Hepatoprotective effects of Monascus pilosus mycelial ethanol extract (MPME) were examined in high-fat diet induced-obese rats. The rats were randomly divided into 2 groups; normal control (NC) and a high-fat and high cholesterol diet group (HFC). The HFC diet group was fed a 5L79 diet supplemented with 15% lard and 1% cholesterol for 3 weeks for induction of obesity. And then, the rats were divided into 4 groups (n=5); the NC, a HFC diet obesity control group (HF), 0.5% MPME supplemented HFC diet group (MPM), and 2% conjugated linoleic acid (CLA) supplemented HFC diet group for 7 weeks. Whereas the daily weight gain of NC and HFC groups were 3.48 g and 4.48 g, respectively, those of MPM and CLA were 3.09 g and 4.38 g, respectively. Furthermore, activity of serum alanine and aspartic aminotransferase in HF was markedly higher than those of NC group, but, the activity in MPM and CLA was significantly lower than HF. Hepatic reduced glutathione content in MPM and CLA was higher than HF. On the contrary, hepatic lipid peroxide content in MPM and CLA was significantly lower than HF. In conclusion, although the precise mechanisms of the hepatoprotective effects of the MPME in this study are unknown, our study provides experimental evidence that MPME may prevent obesity and hepatic damage by high-fat and high cholesterol diet via inhibition of lipid absorption and induction of reactive oxygen spices scavenging enzyme such as superoxide dismutase.

Anti - HBc 단독 양성자에 대한 B 형간염백신의 면역효과

이상인(Sang In Lee),김경희(Kyung Hee Kim),김원호(Won Ho Kim),한광협(Kwang Hyub Han),정재복(Jae Bock Chung),문영명(Young Myoung Moon),강진경(Jin Kyung Kang),박인서(In Suh Park),최흥재(Heung Jai Choi) 대한내과학회 1988 대한내과학회지 Vol.35 No.6

N/A 1mmune responses with hepatitis R vaccine were studied in 13 indivisuals with isolated anti-HBc and 35 indivisuals with negative hepatitis B marker. All healthy volunteers in Severance Hospital were no evidence of hepatitis, history of hepatitis, immunocom-promised state, transfusion, HB vaccination, diabetes mellitus or heavy use of any kinds of drugs. Three doses of Hepavax® 1 ml (HBsAg, 20 mg) were administered intramuscularly in the deltoid muscle at 0, 1 and 2 months, and sera were collected from the subjects at monthly intervals for 3 months after the first injection. All sera were tested for HBsAg, anti-HBs and anti-HBc by radioimmunoassay, and serum aminotransferase. The results were as follows: 1) The anti-HBs response rates in 35 seronegative controls were 60.0% at 1 month after the first vaccination, 74.3% at 2 months and 94.3% at 3 months. Four subjects (11.4%) responded 1 month after vaccination with anti-HBs titers of 50RI.J or greater. 2) The anti-HBs response rate in 13 indivisuals with isolated anti-HBc was 100% from 1 month after the first vaccination. Six subjects (46.2%) responded 1 month after vaccination with antiHBs titiers of 50RU or greater. 3) The isolated anti-HBc group had a significantly higher anti-HBs response rate when compared with seronegative controls at 1 and 2 months but not at 3 months. Anti-HBs titers of isolated anti-HBc group were greater than seronegative controls. 4) There were no abnormal elevation of aminotransferase, no change of HBsAg and anti-HBc for 3 months follow-up. The side effect after vaccination were negligible. In conclusion, there was a tendencv of more rapid anti-HBs response and higher anti-HBs titers in the isolated anti-HBc group than the seronegative group.

대망에 발생한 원발성 평활근육종

이상인(Sang In Lee),김기황(Ki Hwang Kim),남수연(Su Youn Nam),박효진(Hyo Jin Pack),이희대(Hee Dai Lee),정우희(Woo Hee Jung) 대한소화기학회 1993 대한소화기학회지 Vol.25 No.5

Although the greater omentum is frequently the site of metastatic neoplasm, primary omental tumors are exeedingly rare. The authors report here, a case of primary omental leiomyosarcoma presented initially as a palpable abdominal mass and epigastic discomfort for one year in a 66 year-old rnale patient. At laparotomy, 21*15*2.5 cm rnass weighing 600 gram was found in the omentum which was adherent to the anterior wall of the lower body of the stomach and liver surface. In the histologic findings presence of tumor necrosis, increased cellularity, cellular atypia and pleomorphism as well as large size of mass were indicative of leiomyosarcoma. Surg cal resection has been known to be the principle treatment for this disease, In this case, excision of the tumor with omentectomy, radical subtotal gastrectomy and wedge resection of the liver were performed. Eight months later, a recurrence was detected and chemotherapy with doxorubicin, dacarhazine, vincris- tine, and cyclophosphamide was done.

도파민 길항제에 치료 불응성인 기능성 소화불량증 환자에서 시사프리드의 효과

이상인(Sang In Lee),서원태(Won Tae Seo),박인서(In Suh Park),이용찬(Yong Chan Lee),이동기(Dong Gi Lee),이광재(Kwang Jae Lee),김도영(Do Young Kim),신용운(Yong Woon Shin),김영수(Young Soo Kijm),김범수(Pum Soo Kim),김동기(Dong Kee Kim) 대한소화기기능성질환·운동학회 2000 Journal of Neurogastroenterology and Motility (JNM Vol.6 No.2

N/A Background/Aims : Prokinetics are commonly used for the treatment of functional dyspesia, but their methods of action are different. First, we compared the efficacy and safety of the dopamine receptor antagonists, which were domperidone maleate and levosulpiride, in a 2 week treatment in functional dyspepsia, then investigated the efficacy and safety of cisapride tartrate in a 2 week treatment in those who were resistant to domperidone maleate or levosulpiride. Methods : One hundred Forty-nine patients, who were diagnosed with functional dyspepsia, were selected. The subjects were randomly divided into two groups, domperidone maleate (75) and levosupiride (74). Daily they took 30mg of domperidone maleate (DOM) or 75mg of levosulpiride (LEV) for 2 weeks. Then the subjects who didn't respond to these treatments took 30mg of cisapride tartrate for the following 2 weeks. Results : At week 0, the total symptom scores of the DOM group and LEV group were 8.01±2.57 and 8.14±2.65 respectively, which were not statistically different. At week 2, the total symptom scores of the DOM and LEV groups were significantly reduced to 4.28±3.30 and 4.85±3.53(p=0.0001). The efficacy rates of the DOM and LEV groups at week 2 were 50.8% vs. 44.1%. The rate of adverse events in the LEV groups was much higher than in the DOM group(17.7% vs. 8.0%). In addition the rate of change from normal to abnomal in prolactin level was markedly higher in the LEV than that of the DOM group(80.0% vs. 8.3%). After 2 weeks of treatment with cisapride tartrate, the total symptom score was significantly reduced to 3.77±2.49(p=0.0001), and the efficacy rate was 75.0%. The satisfaction of the resistant subjects in efficacy of cisapride compared with the previous treatment was 73.3%. The rate of adverse events of cisapride tartrate was 5.0%. Conclusions: Considering efficacy and safety of domperidone maleate and levosulpiride, domperidone maleate was the safer drug for the treatment of functional dyspepsia, and cisapride tartrate can be a useful drug in those patients who are resistant to dopamine antagonists like domperidone maleate and levosulpiride.

원발병소 및 전이병소에서 배양된 위선암세포의 체외 항암제 감수성 비교

함기백 ( Ham Gi Baeg ),이상인 ( Lee Sang In ),임대순 ( Im Dae Sun ),김원호 ( Kim Won Ho ),문영명 ( Mun Yeong Myeong ),강진경 ( Kang Jin Gyeong ),박인서 ( Park In Seo ),최흥재 ( Choe Heung Jae ),민진식 ( Min Jin Sig ),김충배 ( Kim 대한내과학회 1993 대한내과학회지 Vol.44 No.3

연구배경 : 세균에 대한 항생제 감수성검사와 같은 개념으로 항암제 감수성 검사법이 다양하게 개발, 발전되어 왔으나 아직까지 임상응용에는 많은 제약이 있다. 그러나 감수성검사 결과와 생체내에서의 항암효과 사이에 연관성이 높다는 여러 보고와 함께 실제 생존율도 증가 시킨다는 보고가 있어 항암제 감수성검사의 중요성 및 필요성이 더욱 강조되고 있다. 또한 원발병소 및 전이병소의 암세포는 세포형태, 핵형, 면역성, 항원성, 침습력, 암유전자 발현율 및 항암제 감수성에 있어서 차이가 있는 것으로 알려져 있다. 본 연구의 목적은 원발병소 및 전이병소의 암세포가 항암제 단일 또는 병합에 대하여 감수성에 차이가 있는 지를 알아보고, 체외 항암제 감수성검사를 통하여 가장 감수성이 높은 단일 또는 병합 항암제를 선별할 수 있는지를 알아보는데 있다. 방법 : 위암환자 158예중 138예는 원발병소 (A군)에서, 26예는 전이병소(B군)에서 채취된 위선암세포를 단기간 시험관내에서 배양하였다. 단기간 배양된 암세포를 3-{4, 5-dimethylthiazol-2-yl}-2-5-diphenyl-tetrazolium bromide(MTT) 검사방법을 이용하여 adriamycin(ADR), cisplatin(DDP), 5-fluorouracil(5-FU), mitomycin C (MMC) 및 etoposide (VP-16)의 5가지 단일 또는 병합 항암제에 대한 체외 항암제 감수성검사를 시행하였다. 결과 : 1) 위선암세포의 단기간 배양성공율은 A군에서 138병소 중 20병소로 14.5%, B군에서는 27병소중 18병소로 66.7%로 양군간에 의의있는 차이를 보였다(p<0.001). 2) 배양된 암세포의 위상차 현미경 소견이나 병리학적 도말검사 소견은 양군간에 유의한 형태학적 차이를 관찰할 수 없었다. 3) 각 항암제 각각에 대한 감수성은 환자 개개인에 따라 차이가 많았으며 ADR과 MMC의 IC_50은 B군에서 A군에 비하여, VP-16의 IC_50은 A군에서 B군에 비하여 유의하게 높았다(p<0.05). 4) Additivity model을 이용한 항암제 병합에 대한 감수성검사 결과, 함암제 2제 병합시 A군에서는 ADR+MMC과 ADR+DDP에서, B군에서는 ADR+VP-16, 5-FU+VP-16 그리고 MMC+VP-16에서 유의한 상승효과를 보였으며 (p<0.05), 항암제 3제 병합시 A군에서는 5-FU+ADR+DDP와 MMC+DDP+5-FU에서, B군에서는 5-FU+ADR+VP-16와 VP-16+ADR+DDP에서 유의한 상승효과를 보았다(p<0.01). 5) Isobologram model을 이용한 병합 함암제에 대한 감수성검사 결과, 항암제 2제 병합시 A군에서는 ADR+DDP와 ADR+MMC, B군에서는 ADR+VP-16, MMC+VP-16, DDP+VP-16이, 항암제 3제 병합시 A군에서는 5-FU+ADR+DDP와 MMC+DDP+5-FU, B군에서는 VP-16+ADR+DDP와 5-FU+ADR+VP-16이 대상환자중 70% 이상에서 높은 감수성을 보였다. 6) A군과 B군 사이에 감수성이 높은 병합 항암제는 서로 달라 5-FU+ADR+MMC과 5-FU+ADR+DDP는 A군에서는 상승효과를 보였으나 B군에서는 오히려 길항작용을 보였으며, 5-FU+ADR+VP-16와 VP-16+ADR+DDP는 A군에는 준첨가효과를 보였으나 B군에서는 상승효과를 보였다. 결론 : 원발병소 및 전이병소 위선암세포간에 단일 또 는 병합 항암제에 대한 감수성에 있어서 유의하게 차이가 있었다. 따라서 원발병소 또는 전이병소의 위선암세포를 시험관내에서 단기 배양하여 체외 항암제감수성검사를 시행함으로서 환자 개개인의 위암세포에 가장 감수성이 있는 항암제 선별할 수 있고, 감수성검사의 결과에 따라 선택된 단일 또는 병합 항암제로 화학요법을 시행하면 현재의 경험적인 함암제 투여에 비하여 치료성적이 향상될 것으로 생각된다. Background : Recently, attention has been focused on the possibility of predicting the effectiveness of anticaner drugs against individual tumors through chemosensitivity tests. The MTT assay is a preferable, accurate, and convenient colorimetric assay which is based on the premise that the mitochondria of living cells reduce the tetrazolium salt to formazan. It has been suggested that malignant neoplasms contain multiple cell populations exhibiting tremendous biologic heterogeneity. Particularly the detailed differences in chemosensitivity between cells from primary and from metastasis lesions remain unsolved. This study was designed to assess the usefulness of in vitro chemosensitivity tests in the tratment of gastric cancers and differences in chemosensitivity between gastric cancer cells cultured from primary and metastatic lesions. Method : Primary cancer cells of 138 patients with gastric cancer (group A), obtained through the endoscopic biopsy or surgical open biopsy, and metastatc cancer cells of 26 patients (group B : 27 lesions), obtained through the paracentesis or pleural centesis, were cultured using culture midium supplemented with 20% fetal bovine serum. The colorimetric MTT assay was applied for in vitro chemosensitivity tests. Results : 1) The success rates of short-term culture of gastric cancer cells were 14.5% (20/138) in group A and 66.7% (18/27) in group B, respectively. 2) The morphology of the cultured cells observed through phase-contrast and light microscopy showed no significant difference between group A and B. 3) The mean IC_50 of ADR and MMC were significantly higher in group B than group A (p<0.05) and that of VOㆍ16 significantly higher in group A (p<001) 4) The two-drug combination regimens having thd statistically significant synergistic effects were ADR+MMC (p<0.05) and ADR+DDP (p=0.01) in group a, ADR+VPㆍ16 (p<0.05), 5ㆍFU+VPㆍ16 (p<0.05), and MMC+VPㆍ16 (p<0.05) in group B, and the three-drug combination regimens were 5ㆍFU+ADR+DDP (p<0.05) and MMC+DDP+5ㆍFU (p=0.01) in group A, 5ㆍFU+ADR +VPㆍ16 (p<0.01) and VPㆍ16+ADR+DDP (p<0.01) in group B. 5) The two-drun combination regimens having the supraaddictive effects over 70% of the cases were ADR+MMC and ADR+DDP in group A, ADR+VP-16, 5-FU+VP-16, and DDP+VP-16 in group B, and the three-drug combination regimens 5-FU +ADR+DDP and MMC+DDP+5-FU in group A, 5-FU +ADR +VP-16 and VP-16 +ADR+DDP in group B. 6) The combination regimens having synergistic effects were different between group A and group B. 5-FU +ADR+MMC and 5-FU +ADR+DDP combination had synergistic effects in group A, but antagonistic effects in group B. 5-FU +ADR+VP-16 and VP-16+ADR+DDP combination synergistic effects in group B, but antagonistic effects in group A. Conclusion : The chemosensitivities of gastric cancer cells were significantly different between the primary and metastatic lesions from which the cultured cells were derived. Thus, the individual chemosensitivity test using short-term culture system may be useful for the selection of the most effective anticancer drugs.

Indian head massage가 스트레스와 인체변화에 미치는 효과

이상은(Sang-Eun Lee),박제희(Je-Hee Park),이인희(In-Hee Lee) 한국인체미용예술학회 2012 한국인체미용예술학회지 Vol.13 No.1

To find out the effect of Indian head massage on stress and body changes among young office workers in their twenties and thirties, this study analyzed biological signals and investigated the physical and mental stress and satisfaction of 14 office workers (7 men, 7 women) who have been suffering from hair loss for less than a year from June 13 to July 15, 2011 (for 33 days). In terms of body change (assessment of physical activity, analysis of autonomic balance, diagnosis of central nervous system modulation levels, evaluation of cerebral activity) and changes in physical and mental stress and satisfaction, statistical significance was observed in assessment of physical activity, diagnosis of central nervous system modulation levels and evaluation of cerebral activity while no significant difference was detected in analysis of autonomic balance. In physical and mental stress, improvement was observed in ‘stiffness in the neck or shoulders,’ ‘sleep disorder,’ ‘I can’t get the anxiety out of my head’ and ‘I feel dejected and fatigued.’ In terms of satisfaction, improvement was found in ‘scalp became clean,’ and ‘I feel fewer hot flashes on my scalp.’ Therefore, it is expected that the experimental model proposed in this study, with which the effect of Indian head massage on the improvement of quality of life and body changes among office workers in their twenties and thirties could be used in various fields and helpful in improving quality of life and health. Further study on the model is required.

당뇨병환자에서 식도운동에 관한 연구

이상인(Sang In Lee),이관우(Kwan Woo Lee),임승길(Seung Kil Lim),이현철(Hyun Chul Lee),문영명(Young Myoung Moon),최흥재(Heung Jai Choi) 대한내과학회 1988 대한내과학회지 Vol.34 No.3

N/A Esophageal manometry was performed in 10 diabetics with neuropathy, 11 diabetics without neuropathy and 11 healthy controls using low compliance pneumohydraulic capillary infusion system before and after infusion of edrophonium (80 ㎍/kg) and atropine (3 ㎍/kg). The results were summarized as follows: 1) Basal lower esophageal sphincter pressure and relaxation in diabetics were similar to that of healthy controls. 2) There were no significant differences in the amplitude, duration and velocity of peristaltic wave between diabetics and controls. 3) Multipeaked peristaltic waves were observed in 9 of 10 diabetic patients with neuropathy and more than quadriple peaked waves were 30%. Multipeaked peristaltic pressure complexes in control differed from those present in diabetics with neuropathy by their low incidence and by a tendency to be limited to the distal esophagus. In conclusion, multipeaked peristaltic pressure waves were the significant manmometric finding in diabetics with neuropathy and phamacologic response to edrophonium and atropine suggested the increased cholinergic tone.

Monascus pilosus로 발효시킨 비지의 항비만 효과

이상일 ( Sang Il Lee ),이예경 ( Ye Kyung Lee ),김순동 ( Soon Dong Kim ),이인애 ( In Ae Lee ),최종근 ( Jongkeun Choi ),서주원 ( Joo Won Suh ) 한국응용생명화학회(구 한국농화학회) 2014 Journal of Applied Biological Chemistry (J. Appl. Vol.57 No.1

본 연구에서는 고지방식이로 비만을 유도한 마우스를 이용하여 비지 (SCR)와 Monascus pilosus로 발효비지의 항비만 및 항산화 효능을 조사하였다. 마우스는 비지와 발효비지를 2% (wt/wt)가 되도록 고지방식이에 첨가하여 8주간 사육하였다. 비지 및 발효비지 투여군은 고지방식이 대조군과 비교할 때 모두 유의한 수준의 체중 감량, 체중 증가율, 부고환주변의 지방 함량이 낮았다. 8주째의 초기체중에 대한 체중증가율은 정상식이군19.54%, 고지방식이군 45.40%, 비지군 33.39% 그리고 발효비 지군 31.42%로 발효비지군에서 가장 낮았다. 또한 혈청의triglyceride, 총 콜레스테롤, LDL-콜레스테롤 함량 그리고 간 조직의 trigrlyceride 함량도 고지방식이로 증가된 수준에서 크게 줄어들었다. 한편 비지와 발효비지의 급여는 glutathione 함량을 높이고, 지질의 과산화, 혈청의 aminotransferase 및 xanthineoxidase의 활성을 낮추는 것으로 나타났다. 반면, superoxide dismutase, glutathione S-transferase, glutathione peroxidase의 활성은 비지 및 발효비지군 모두에서 고지방식 이군에 비하여 높았다. 마지막으로, 비지군과 비교할 때 발효비지군은 고지방식이로 비만을 유도한 마우스에서 비정상적인 비만 관련 생화학적 지표들을 좀 더 효과적으로 정상식이군의 지표들로 회복시켜주는 것을 확인할 수 있었다. 이상의 결과, 고지방 식이로 인한 체중 증가와 복부지방의 축적 및 고지혈증과 간 조직의 지방 축적현상이 M. pilosus로 발효시킨 비지의 2% 식이로 항비만 효과와 동시에 혈액 및 간 조직 지방의 함량을 저하시켜 고지혈증으로 인해 야기될 수 있는 심혈관계 질환을 예방할 수있을 것으로 생각된다. 또한 활성산소 생성계의 활성 억제와 활성산소 소거계 활성 증가를 유도함으로서 비만 상태에서 나타날 수 있는 과잉의 활성산소에 의한 간 조직 손상을 예방 혹은 경감시켜줄 수 있을 것으로 사료된다. This study investigated anti-obesity and antioxidant effects of dietary non-fermented soybean crud residue (SCR) and fermented SCR by Monascus pilosus (FSCR) in high-fat inducedobese mice. SCR and FSCR were supplemented with high-fat diet at 2% (wt/wt) dose for 8 weeks. Both SCR and FSCR significantly lowered body weight, epididymal fat weight and weight gain rate compared to high-fat diet control (HC) group and FSCR group showed lowest weight gain rate. In addition, it was observed that serum and hepatic lipid profiles including triglyceride, total cholesterol, LDL-cholesterol and HDL-cholesterol were significantly improved by supplementing SCR or FSCR. Furthermore, SCR and FSCR administration showed increase of glutathione content and decrease of hepatic lipid peroxide content, serum aminotransferase activity, and hepatic xanthine oxidase activity. On the other hand, activities of reactive oxygen species scavenging enzyme such as superoxide dismutase, glutathione S-transferase and glutathione peroxidase in two test groups were higher than those of HC. Lastly, in comparison with SCR, FSCR was more effective in restoring obesity-related biomarkers to normal level in high-diet induced obese mice. In conclusion, the present study indicates that FSCR could have not only anti-obese effects such as inhibition of abdominal fat accumulation, but also protective effects of cardiovascular disease such as atherosclerosis by decreasing serum and hepatic lipid contents. Furthermore, these results suggest that experimental diets in this study could alleviate hepatic damage caused by overproduction of reactive oxygen spices (ROS) due to obesity via inhibition of ROS generating activities and induction of ROS scavenging activities.

Monascus pilosus 발효 뽕잎차가 고지방 식이 마우스의 체중과 간 조직 항산화계 효소 활성에 미치는 영향

이상일 ( Sang Il Lee ),이예경 ( Ye Kyung Lee ),이인애 ( In Ae Lee ),최종근 ( Jong Keun Choi ),김순동 ( Soon Dong Kim ),서주원 ( Joo Won Suh ) 한국식품영양학회 2013 韓國食品營養學會誌 Vol.26 No.1

본 연구에서는 뽕잎차와 Monascus pilosus로 발효시킨 뽕잎차가 ICR mouse의 체중과 간 조직 항산화계 효소류의 활성에 미치는 영향을 조사하기 위하여 정상대조군(NC), 고지방식이 대조군(HC), 뽕잎차 분말을 2% 혼합한 고지방 식이군(UM), 발효 뽕잎차 분말을 2% 혼합한 고지방 식이군(FM)으로 나누어 8주간 사육하였다. 체중증가량에 있어서는 HC군의 주당 체중증가량은 NC군에 비해 2.13배로 높았으나, UM군과 FM군은 NC군의 1.80 및 1.67배로 체중증가량이 크게 완화되었다. 장기 중량은 고지방 식이군에서 증가되었던 간의 중량은 UM과 FM군에서 회복되는 경향을 보였으며, 신장의 경우에도 NC군 수준으로 회복되는 경향을 나타내었다. 부고환 주변 지방의 함량은 UM과 FM군의 경우 모두 고지방식이로 현저하게 증가된 지방함량을 유의적으로 감소시켰으며, 그 효과는 FM군에서 현저하였다. ALT 활성은 HC군이 NC군에 비하여 2.56배나 높은 수치를 나타내었으며, UM군과 HC 간의 유의차는 보이지 않으나 FM군은 HC군에 비하여 유의적으로 낮았다. TG, TC, LDL-cholesterol의 함량 및 동맥경화지수(AI)는 HC군은 NC군에 비하여 각각 18.42%, 39.59%, 212.56% 및 230.70%가 증가하였으며, HDLcholesterol 함량은 17.84%가 감소하였다. UM군의 TG, TC, HDL- 및 LDL-cholesterol 함량은 HC군과 유의적인 차이가 없으나, FM군에서는 유의적인 차이를 보였으며, 동맥경화지수도 HC군 2.63, UM군 2.09, FM군 1.70으로 FM군에서 양호한 결과를 나타내었다. 간 조직 TG 함량은 UM 및 FM군이 HC군에 비하여 25.18∼25.72%가 감소되었으며, 총 콜레스테롤의 함량에서도 TG와 유사한 경향을 보였다. Glutathione(GSH)의 함량은 FM군이 UM군에 비하여 유의적으로 증가하였으며, lipid peroxide(LPO)의 함량은 UM군과 FM군은 HC군에 비하여 각각 49.82% 및 44.52% 감소하였다. Xanthine oxidase(XO) 활성은 UM군과 FM군에서는 HC군에 비하여 각각 38.41% 및 53.62%가 감소 하였다. SOD 활성은 UM과 FM군 모두 NC군과 유사한 활성을 나타내었다. Glutathione S-transferase(GST)활성은 UM군과 FM군은 고지방 식이에 의해 감소된 HC군의 활성을 14.81∼23.46% 증가시켰다. Glutathione peroxidase(GPX) 활성(NADPH nmole/min/mg protein)은 고지방 식이 대조군인 HC군에서의 활성은 NC군의 65.59%에 불과하였으나, UM군과 FM군은 HC군에 비하여 각각 22.95% 및 38.52% 증가하였다. 간 조직을 검경한 결과, 고지방 식이 대조군인 HF군에서는 간 조직 세포가 지방 방울의 축적에 의해 확장되어 간굴모세혈관(hepatic sinusoids)이 폐쇄된 상태였다. 하지만, UM군과 FM군에서는 중심정맥 주위의 세포들이 정상적으로 잘 보존되어 있으며, 간 소엽의 간세포삭과 간 조직 세포 내에 지방방울의 축적이 보이긴 하나 구분이 가능하며, UM군에 비하여 FM군 쪽의 간 손상이 보다 경미한 것으로 나타났다. 이상의 실험결과, M. pilosus로 발효시킨 뽕잎차는 정상식이군에서는 간 조직 손상과 관련된 LPO의 함량을 경감시키는 효과와 함께 경미한 체중 감소 효과가 있는 것으로 사료되며, 고지방 식이에 의한 비정상 생리 상태에서는 비만 해소와 ROS 생성계효소의 저해와 소거계 항산화 효소의 활성화를 통한 간 기능 증진과 조직 손상을 예방 또는 경감시키는 효과가 있는 것으로 생각된다. In this study, we investigated the preventive effects of the mulberry leaf tea fermented by Monascus pilosus on high fat-induced obesity, hyperlipidemia, and fatty liver in mice. Non-fermented mulberry leaf tea powder (UM) and fermented mulberry leaf tea powder (FM) were supplemented with high-fat diet at 2% (wt/wt) dosage for 8 weeks. Both UM and FM lowered body weight gain, feed efficiency ratio, epididymal fat, serum triglyceride, total cholesterol and LDL-cholesterol increased markedly with high fat diet (HC) in mice. FM showed more significant effects when it was compared with UM. In addition, Hepatic lipid peroxides and xanthin oxidase activities of the UM and FM were significantly lower than those of HC, despite the lack of a big difference in the amount of hepatic GSH. Activities of ROS scavenging enzymes and serum alanine aminotransferase activity were also examined as a parameter of hepatic damage. The UM and FM groups showed a recovery to NC group from significant changes induced by HC. Finally, histopathological analyses of liver samples revealed a decrease of lipid accumulation in hepatocytes in the UM and FM groups. These results suggest that UM and especially FM can reduce the development of obesity, hyperlipidemia and fatty liver.