인간포배기 배아의 효과적인 유리화 동결법의 개발을 위한 연구

이상민,이주희,이상원,이승민,윤산현,임진호,박흠대,이성구,Lee, Sang-Min,Lee, Ju-Hee,Lee, Sang-Won,Lee, Seoung-Min,Yoon, San-Hyun,Lim, Jin-Ho,Park, Huem-Dai,Lee, Seong-Goo 대한생식의학회 2003 Clinical and Experimental Reproductive Medicine Vol.30 No.3

Objective: The purpose of this study was to evaluate the survival rate of vitrified blastocyst according to the freezing vessels, equilibration time in cryoprotectant and artificial dehydration method. Methods: Human blastocysts were vitrified after loading onto the plastic straw, open-pulled straw (OPS), electron microscopy grid (EM grid) for 1.5 min or 3 min. They also were directly plunged into LN2 within 30sec. For artificial shrinkage of blastocysts, 36 gauge fine needle was pushed at the cellular junction of the trophectoderm into the blstocoele cavity until it shrank without damage of inner cell mass. Results: The survival rate of vitrified blastocysts on plastic straw, OPS, EM grid as freezing vessels were 26.7, 13.0 and 60.5%, respectively. The survival rate of EM grid was significantly higher than that of plastic straw and OPS (p<0.05). For 1.5 min equilibrium, the survival rates of early blastocyst (EB), middle blastocyst (MB) and late blastocyst (LB) were 64.4, 81.0, and 20.0% respectively. For 3 min equilibrium, the survival rates of EB, MB, and LB were 69.9, 50.0 and 57.5% respectively. The survival rates of EB and MB were significantly higher than that of LB in 1.5 min equilibrium group (p<0.05), however, the significance was not observed in 3 min equilibrium groups. In cytoplasmic shrinkage before vitrification, the survival rates of EB, MB and LB were 92.9, 100 and 75.9% respectively. The survival rate of MB was significantly higher than that of LB (p<0.05). The survival rates of vitrified blastocysts by artificial dehydration and slow-frozen blastocysts were not significantly different as 88.9 and 66.7%, respectively. Conclusion: This study showed that the vitrification of human blastocysts using EM grid and artificial dehydration is an effective method. Therefore, these methods would be an useful techniques for blastocyst cryopreservation.

녹비작물을 이용한 윤작이 잡초 제어에 미치는 영향

이상민,이병모,이연,이용환,성좌경,윤홍배,최현석,Lee, Sang-Min,Lee, Byung-Mo,Lee, Youn,Lee, Yong-Hwan,Sung, Jwa-Kyung,Yun, Hong-Bae,Choi, Hyun-Sug 한국유기농업학회 2012 韓國有機農業學會誌 Vol.20 No.2

본 시험은 유기농업 파 및 고추재배를 위하여 호밀과 헤어리베치를 겨울에 윤작하여 잡초 발생량 억제에 미치는 효과를 검토하기 위하여 수행되었다. 파 재배구에서 호밀을 토양에 환원하지 않은 처리구에 비해 호밀을 환원하였을 경우 잡초발생량을 52% 경감시켰다. 고추 재배구에서는 그러한 효과가 뚜렷하게 관찰되지 않았다. 파 재배시 어저귀, 참방동사니, 바랭이와 같은 초종은 호밀뿌리 분비물에 의해 발아가 억제되는 경향이었으나 헤어리베치는 그러한 발생억제 효과가 크지 않았다. 파 재배시 호밀 환원은 잡초발생량을 경감시켰고 잡초의 종 다양성 지수를 증가시켰다. This study was conducted to evaluate the effects of crop rotation on the weed occurrence in green onion and red pepper cultivation. Rye and hairy vetch were annually seeded for winter season. The reduction of weed germination was pronounced by about 50% in green onion field, but was not significant in red pepper field through rye cropping. Among weed species, the germination of Abutilon theophrasti, Cyperus iria and Digitaria ciliaris was distinctly inhibited by exudate of rye root, but not by that of hairy vetch in vitro experiment. The return of rye biomass into onion field decreased amount of weed biomass but increased index of weed diversity.

헤어리베치(Vice villosa Roth) 품종별 국내 월동성, Biomass 및 무기태 질소 함량비교

이상민,이연,이용환,성좌경,윤홍배,최현석,Lee, Sang-Min,Lee, Youn,Lee, Yong-Hwan,Sung, Jwa-Kyung,Yun, Hong-Bae,Choi, Hyun-Sug 한국유기농업학회 2013 韓國有機農業學會誌 Vol.21 No.1

헤어리베치 8종의 품종별 국내 적응성 검증을 위해 월동률과 biomass, 그리고 토양 중 무기태질소를 조사하였다. 겨울철 월동 후 생존율은 Hungvillosa와 Ostsaat 품종에서 좋았고, Minnie, Oregon common과 TTF1에서 낮았다. 생존율은 biomass에도 영향을 주어서 Hungvillosa와 Ostsaat 품종에서 가장 높았다. 헤어리베치 품종 간 무기성분 농도는 별다른 차이가 관찰되지 않았다. 토양 중 무기태질소 농도는 질소환원능이 좋았던, Hungvillosa와 Ostsaat가 예초 후 10여일 후에 크게 증가시켰다. This study was conducted to evaluate overwintering of hairy vetch (Vicia villosa Roth), biomass, and inorganic N concentration in soil to verify the adaptability of eight cultivars of hairy vetch into country. Winter survival rate was higher for the hairy vetch cultivars, such as Hungvillosa or Ostsaat, than those of Minnie, Oregon common, and TTF1, which affected amount of biomass of each hairy vetch, with higher biomass observed in Hungvillosa or Ostsaat cultivar. There were no significant difference for the mineral nutrients of each hairy vetch cultivar. Inorganic N concentration in soil was increased at 10 days after mowing by application of Hungvillosa and Ostsaat cultivars that had highly produced N content from the raw materials.

『동아시아의 아카이브 비교연구』 곽건홍 저, 도서출판 선인, 2016

이상민,Lee, Sang-Min 한국기록학회 2016 기록학연구 Vol.0 No.49

학교시설 수선주기 설정에 관한 연구

이상민,Lee, Sang Min 한국교육녹색환경연구원 2018 교육·녹색환경연구 Vol.17 No.2

본 연구는 안전성, 노후도, 경제성 등 시설 여건을 종합적으로 고려하여 개선이 필요한 중장기적인 사업물량 및 사업예산 규모를 추정할 수 있도록 하기 위해 국가수준의 시설개선 기준(수선주기)을 설정하여 체계적인 시설개선계획 수립을 통한 적정수준의 교육환경 확보에 기여할 수 있도록 하는 것을 주목적으로 한다. 적정 수선주기는 기본적으로 최근 2년간 교육청에서 실제 집행한 교육환경개선사업의 실적자료를 분석하여 직전 보수년도와 사업시행연도를 근거로 수선주기 기초자료를 산출하였다. 이를 근거로 17개 시도교육청 실무자를 대상으로 델파이 조사를 실시하여 시도교육청의 의견을 수렴하였으며 최종 적정 수선주기는 관련규정과 실적자료, 델파이조사 결과를 종합적으로 검토하여 설정하였다. In Korea, it is difficult to secure student health and safe educational environment due to the rapid deterioration of many school facilities built in the 70s and 80s in the past. As a result, through the improvement of school facilities, social needs for safe schools from various disasters, accidents, Is increasing. The purpose of this study was to establish a standard for the establishment of the national and school facilities for the mid - to long - term plan for the improvement of the old school facilities, and to propose a framework for mid - to long - term and rational planning that can be used by the national and local government offices. The purpose of this study is to establish a mid - and long - term plan for the improvement of old school facilities. The appropriate repair cycle for the improvement of the school facilities set up in this study can be utilized as a standard for the mid - and long - term planning for improvement of the school facilities in the Ministry of Education and the School of Education. To this end, a standard model, It is possible to roughly estimate the overall improvement in the mid- to long-term facilities at the national and regional levels.

회전방지장치와 지대주의 내육각구조가 임플란트로 전달되는 조임 회전력에 미치는 영향

이상민,전영찬,정창모,Lee Sang-Min,Jeon Young-Chan,Jeong Chang-Mo 대한치과보철학회 2003 대한치과보철학회지 Vol.41 No.2

Statement of problem : Little is known about the effect of a counter-torque device and the internal hexagon of abutment on the tightening torque transmitted to the implant. Purpose : The purpose of this study was to examine the effect of a counter-torque device and the internal hexagon of abutment on the tightening torque transmitted to the implant. Material and Methods : In this study, three types of abutment were used, (1) two-piece conical abutment with hexagon, (2) two-piece conical abutment without hexagon, and (3) one-piece conical abutment without hexagon. The experimental groups were divided into five groups according to the type of abutment and the usage of a counter-torque device. Group I : two-piece conical abutment with internal hexagon was tightened without the use of a counter-torque device. Group II : two-piece conical abutment without internal hexagon was tightened without the use of a counter-torque device. Group III : one-piece conical abutment without internal hexagon was tightened without the use of a counter-torque device. Group IV : two-piece conical abutment with internal hexagon was tightened with the use of a counter-torque device Group V : two-piece conical abutment without internal hexagon was tightened with the use of a counter-torque device. Abutments were tightened 20Ncm torque with the use of manual torque wrench and then torque values were measured by torque-gauge. After the measurement of torque values, all groups were loosened with the use of manual torque wrench and then detorque values were measured by torque-gauge. Results : The results were as follows. 1. There were no differences in torque values among three types of abutment. 2. Regardless of the existence of the internal hexagon of abutment, a counter-torque device decreased the tightening torque transmitted to the implant about 92% 3. In group III showed the highest detorque value, however there were no differences among group I, II, IV and V. Conclusion : Within the limitations of this study, it was concluded that the internal hexagon of abutment has no effect on the tightening torque transmitted to the implant and the detorque value of abutment screw. The use of a counter-torque device is essential to prevent microfracture on the implant-bone interface but has no effect on preload.

CIGS 박막의 전착에 관한 연구

이상민,김영호,오미경,홍석인,고항주,이치우,Lee, Sang-Min,Kim, Young-Ho,Oh, Mi-Kyung,Hong, Suk-In,Ko, Hang-Ju,Lee, Chi-Woo 한국전기화학회 2010 한국전기화학회지 Vol.13 No.2

Cu(In, Ga)$Se_2$ (CIGS) 박막은 다원화합물이기 때문에 제조공정이 매우 까다롭다. 진공장치를 사용하는 제조 방법으로 동시증착법, 스퍼터링법 +셀렌화가 있고, 비진공 제조 방법으로 전기화학적인 전착법이 있다. 각 방법에 있어서도 출발 물질의 종류에 따라 다양한 제조 방법이 동원 될 수 있다. 진공증착에 의한 방법은 고품질의 박막을 얻는데 사용 되고 있으나 고가의 진공장비가 사용되므로 대면적화에 따른 제조비용 측면에서 문제가 있다. 이에 비하여, 전착법은 간단하면서도 저가로 대면적화를 이룰 수 있다는 장점 때문에, 많은 관심이 기울여지고 있다. 본 연구에서는 Mo/Glass전극위에 Ga/(In + Ga) = 0.3의 성분비를 만족시키는 CIGS 박막을 전기화학적으로 제조하기 위하여, $Cu^{2+}$, $In^{3+}$, $Ga^{3+}$, $Se^{4+}$ 4성분을 모두 포함하는 전해질 수용액 내에서, 4성분의 이온들이 동시에 환원되는 전위를 조절하여 CIGS 박막을 전착 하였다. SEM을 이용하여 얻어진 CIGS 박막의 전착된 시료의 표면을 관찰하였고, EDS로 그 조성을 분석하였다. 또한, XRD를 이용하여 전착시료의 열처리 전후의 결정성변화를 조사하였다. The chalcopyrite $Cu(In_xGa_{(1-x)})Se_2$ (CIGS) is considered to be one of the effective light-absorbing materials for thin film photovoltaic solar cells. We describe the electrodeposition of CIGS thin films in ambient laboratory conditions, and suggest the electrochemical conditions to prepare stoichiometric CIGS thin films of Ga/(In + Ga) = 0.3. In acidic solutions containing $Cu^{2+}$, $In^{3+}$, $Ga^{3+}$ and $Se^{4+}$ ions, the CIGS films of different Cu/In/Ga/Se chemical compositions were electrodeposited onto Mo/Glass substrate. The structure, morphology and chemical composition of electrodeposited CIGS films were characterized by X-ray diffraction (XRD), Scanning electron microscopy (SEM), and Energy dispersive X-ray spectroscopy (EDS), respectively.

휴대용 GPS를 이용한 부선의 안전예항시스템 구축에 관한 연구

이상민,안병길,Lee, Sang-Min,Ahn, Byung-Kil 해양환경안전학회 2008 海洋環境安全學會誌 Vol.14 No.4

피예선인 부선으로 인하여 예선 자체의 조종성능에 제한을 받고, 이러한 예부선은 바람, 파도, 조류 등과 같은 외력의 영향이 크게 작용하여 예부선 운항의 안정성 확보에 많은 어려움이 수반되고 있다. 예선의 선장, VTS 및 육상의 운항부서 등에서는 부선과 같은 피예선의 실해역에서의 침로, 속도, 위치 등을 실시간으로 추적하여 해난사고를 사전에 예방할 수 있는 시스템의 구축이 절실히 필요한 실정 이다. 본 연구에서는 비교적 저렴하고 이동이 용이한 휴대용 GPS를 이용한 부선의 안전예항시스템의 구축에 대한 기초적인 방법을 제안하고, 이를 토대로 실선에서의 테스트를 실시하고 그 결과에 대하여 논의한다. 본 연구를 통하여 안전 예항에 필요한 여러 항해 정보를 취득할 수 있었으며, 이러한 정보는 다양한 분야에서의 활용이 가능할 것으로 사료된다. The tug boat is restricted in her maneuvering ability due to the towed barge, and tug-barge has been strongly affected by the external forces, i.e. the wind, wave, currents, and so on. Therefore, it is difficult to get the safety of tug-barge operation. It is necessary for the captain of tug boat, VTS and operation department to develope the system which can keep observing the course, speed and position of the towed barge in actual sea and prevent the marine accidents in advance. In this study, we proposed the basic method to develope the safety towing system for barge using portable GPS which was easily movable and relatively inexpensive. Then we conducted the test on the real barge. As a result, we could obtain diverse navigation data to make a safety towing work. It was noted that these data could be used for carious purposes.

아키비스트 윤리 규약과 논평

이상민,Lee, Sang-Min 한국기록학회 2003 기록학연구 Vol.0 No.7

교육에서의 소진에 관한 이론적 고찰

이상민,안성희,Lee, Sang Min,An, Sunghee 연세대학교 의과대학 2014 의학교육논단 Vol.16 No.2

Burnout research has mostly been performed in human service fields. However, it has recently been expanded to cover diverse settings and even non-occupational samples, such as students. In this study, we defined the construct of academic burnout, which is the burnout experienced by students. Next, we described the concept of academic burnout by introducing measurements for assessing academic burnout. On the basis of the demand-control model (DCM) and effort-reward imbalance model (ERIM), which are the most predominant theoretical burnout models, we described the causal factors and the pathway to experiencing academic burnout symptoms. The ERIM was a more influential model than the DCM when explaining the academic burnout of Korean students. Based on the results of previous longitudinal studies on academic burnout, we recognized emotional exhaustion and academic inefficacy as the initial symptoms of academic burnout. Finally, we discussed the prevention and intervention programs with specific components that should be included in those programs.