한국산 거머리로부터 항혈전단백질의 검색과 분리 , 정제

이상권,손정훈,최의성,이상기 ( Sang Kwon Lee,Jung Hoon Sohn,Eui Sung Choi,Sang Ki Rhee ) 생화학분자생물학회 1993 BMB Reports Vol.26 No.3

Two Korean leech species, Hirudo nipponia and Whitmania sp., were examined for the presence of anticoagulant activity. Crude extracts of the leech head parts were fractionated by ethanol and the anticoagulant activity of these extracts was assessed with respect to the inhibition of thrombin, factor Xa and trypsin using the chromogenic substrate assay. While the extract from Hirudo nipponia inhibited all three serine proteases employed in the assay, that of Whitmania sp. inhibited only trypsin, but not thrombin and factor Xa. Thus, the extract from Hirudo nipponia which shows antithrombin activity was subjected to further purification. Three steps of column chromatography including gel filtration on Superose 12 column, anion exchange on Mono-Q and reverse phase HPLC on C-8 column could resolve 3 antithrombin activity peaks from anti-trypsin and anti-factor Xa activities, indicating the presence of multiple forms of thrombin-specific inhibitors in Hirudo nipponia. N-terminal amino acid sequences of the two of these forms showed a limited degree of homology with a portion of heparin cofactor Ⅱ.

Thinking Science 프로그램의 적용에서 지연검사에 의한 중학생들의 인지발달 가속 효과의 분석

이상권,최병순,신애경,이종백,백명화,Lee, Sang-Kwon,Choi, Byung-Soon,Shin, Ae-Kyung,Ree, Jong-Baik,Paek, Myeong-Hwa 대한화학회 2007 대한화학회지 Vol.51 No.6

이 연구의 목적은 중학교 학생들에게 Thinking Science 프로그램을 통한 실험집단과 통제집단을 3 년에 걸쳐 비교하여 인지가속 효과를 조사하는 것이다. 이 프로그램은 2년간 중학교 1학년 168명의 학생들에 게 투입되었다. 프로그램 투입 이후에 SRT VII으로 사후검사와 지연검사를 실시한 결과 학생들의 형식적 사 고 능력의 향상이 거의 2배나 이루어졌다. 사후검사에서 실험집단의 인지발달 수준이 통제집단과 비교하여 유 의미한 인지향상점수를 얻었다. 투입이 더 이상 이루어지지 않은 다음 해의 지연검사에서는 실험집단과 통제 집단이 동일한 비율로 인지발달이 이루어졌지만 실험집단의 구체적 조작 후기에 있는 많은 학생들이 형식적 조작기로 이동하였다. 성별에 따른 인지수준의 향상은 사후검사에서는 실험집단의 여학생들이, 그리고 지연검 사에서는 남학생들이 형식적 조작기로의 이동 비율이 더욱 크게 나타났다. The purpose of this study was to investigate cognitive acceleration effect of the experimental group over three years compared with the control group through the ‘Thinking Science' program in the middle school students. The ‘Thinking Science' program was implemented to 168 students in 7th grade over a period of two years. After implementation of the ‘Thinking Science' program, the improvement of formal thinking ability of the students was almost twice examined with SRT VII as the post-test and the delayed test. It was shown that by the end of the implementation period the experimental group had achieved a significantly greater gain score in levels of cognitive development than had the control group. In the subsequent year during which there was no further implementation, the experimental group continued to develop at the same rate as the control group. However, it is clear that much more experimental students in mature concrete operational stage shifted to formal operational stage in delayed test. The results were also analyzed by gender, and cognitive levels of the students. In the post-test, the ratio of girls that shifted to formal operation stage in the experimental group increased as compared with the ratio of boys, while, in the delayed test cognitive level of boys was more effective than that of girls.

Anti-apoptotic Activity of Heme Oxygenase-1 Up-regulated by Etoposide in Human Retinal Pigment Epithelial Cells

이상권,송주동,김강미,김종민,이상률,유영현,박영철,Lee, Sang-Kwon,Song, Ju-Dong,Kim, Kang-Mi,Kim, Jong-Min,Lee, Sang-Yull,Yoo, Young-Hyun,Park, Young-Chul Korean Society of Life Science 2007 생명과학회지 Vol.17 No.9

The topoisomerase II inhibitor etoposide causes an accumulation of DNA double strand breaks within the nuclei of cells. In this study, we investigated the effect of etoposide on the cell growth and apoptosis of human RPE cells. Etoposide evoked a significant inhibition of cell growth, and also induced DNA fragmentation in ARPE-19 cells. In addition, etoposide significantly up-regulated the expression of heme oxygenase-1 (HO-1), which is a stress-responsive protein and is known to play a protective role against the oxidative injury. And, etoposide-induced HO-1 expression was affected by the ROS scavenger N-acetyl cysteine. We also used oligonucleotides interfering with HO-1 mRNA (siRNA) for the inhibition of HO-1 expression. Interestingly, knock-down of the HO-1 gene significantly increased the level of DNA fragmentation in etoposide-treated ARPE-19 cells. In conclusion, these results suggest that up-regulated HO-1 plays as an anti-apoptotic factor in the process of apoptosis of ARPE-19 cells stimulated by etoposide. Totopoisomerase II 저해제인 etoposide는 핵안에 DNA double strand breaks를 일으키므로써 세포의 DNA에 손상을 초래한다. 본 연구에서는 인간 망막색소상피세포인 ARPE-19 세포에서의 세포성장 및 아폽토시스에서 etoposide의 역할을 살펴보았다. Etoposide는 세포의 성장을 크게 감소시켰으며 TUNEL에서 아폽토시스를 나타내는 DNA fragmentation의 증가를 유도하였다. 게다가, etoposide는 산화적 손상에 대해 세포나 조직을 보호하는 역할을 하는 것으로 알려진 세포내 항산화효소인 heme oxygenase-1 (HO-1)의 발현을 크게 증가시켰다. Etoposide에 의한 HO-1 발현증가는 항산화물질 NAC에 의해 억제되었는데, 이는 etoposide에 의한 세포내 ROS의 증가가 HO-1 발현에 중요한 역할을 한다는 것을 의미한다. 또한 HO-1 발현을 억제하기 위하여 HO-1 siRNA 방법을 사용하였다. 흥미롭게도, HO-1 유전자의 knock-down은 etoposide에 의해 유도되는 DNA fragmentation의 정도를 증가시켰다. 이들 결과를 종합해볼 때, etoposide에 의해 자극되어진 ARPE-19 세포에서 발현증가된 HO-1은 etoposide에 의한 아폽토시스 유발과정에서 세포를 보호하는 항아폽토시스의 기능을 한다는 것을 시사한다.

소프트웨어 분산공유메모리시스템을 위한 적응적 선인출 기법

이상권,윤희철,이준원,맹승렬,Lee, Sang-Kwon,Yun, Hee-Chul,Lee, Joon-Won,Maeng, Seung-Ryoul 한국정보과학회 2001 정보과학회논문지 : 시스템 및 이론 Vol.28 No.9

공유가상메모리 시스템은 적은 비용으로 고성능 계산 능력을 제공하지만, 메모리 접근 지연 시간이 길다는 문제점이 있다. 일반적으로 이 지연시간은 공유데이터에 대한 반복적인 무효화 작업에 의해 일어난다. 고유 데이터들은 동기화를 통해서 접근되고 쓰레드를 반복적 패턴에 의해 동기화 되기 때문에, 반복성에 기반한 선인출 기법은 메모리 지연시간을 효과적으로 줄일수 있다. 본 논문에서는 동기화 변수별로 접근 기록을 분석해서 미래의 메모리 접근을 예측하는 선인출 기법을 제안한다. 제안하는 기법은 8노드 클러스터상에서 SPLASH-2 응용들을 실행시켜 성능을 측정하였다. 그 결과, 제안하는 기법이 34%~45%정도의 메모리 접근 지연시간을 감소할수 있었다. Though shared virtual memory (SVM) system promise low cost solutions for high performance computing they suffer from long memory latencies. These latencies are usually caused by repetitive invalidations on shared data. Since shared data are accessed through synchronization and the patterns by which threads synchronizes are repetitive, a prefetching scheme bases on such repetitiveness would reduce memory latencies. Based on this observation, we propose a prefetching technique which predicts future access behavior by analyzing access history per synchronization variable. Our technique was evaluated on an 8-node SVM system using the SPLASH-2 benchmark. The results show the our technique could achieve 34%~45% reduction in memory access latencies.

상악 제1소구치에서 전부도재관의 finish line 형태에 따른 파절강도와 응력 분포에 관한 연구

이상권,동진근,Lee, Sang-Kwon,Dong, Jin-Keun 대한턱관절교합학회 2003 구강회복응용과학지 Vol.18 No.2

he purpose of this study was to compare the fracture resistance of the IPS Empress ceramic crown with 1.0mm width rounded shoulder, which is usually recommended in all ceramic crown, and 0.5mm width chamfer finish lines on the maxillary first premolar. 30 sound maxillary first premolars were selected and then storaged in 5% NaOCl and saline. 15 teeth were performed preparation for each group(1.0mm rounded shoulder, 0.5mm chamfer). After 30 stone dies were made for each group, the IPS Empress ceramic crowns were fabricated and cemented with resin cement(Bistite resin cement, Tokuyama Soda Co. LTD., Japan) on the natural teeth. The cemented crowns were mounted on the positioning jig and the universal testing machine(Zwick Z020, Zwick Co., Germany)was used to measure the fracture strength, with stress loading on the occlusal surface between buccal and lingual cusp. And also, three-dimensional finite element model was used to measure the stress distribution with two types of the finish lines(1.0mm rounded shoulder, 0.5mm chamfer) and two loading conditions(both buccal and lingual cusp inclination, lingual cusp inclination only). The result of the this study were as follows. In the fracture resistance experiment according to the finish line, the mean fracture strength of rounded shoulder(842N) showed higher value than that of the chamfer(590N) (p<0.05). In the three dimensional finite element analysis of all ceramic crown, metal die and natural teeth model did not show any differences in stress distribution between finish lines. Generally, when force was loaded on the occlusal inclination of buccal and lingual cusp, the stress was concentrated on the loading point and the central groove of occlusal surface. When force was loaded only on the occlusal inclination of lingual cusp, the stress was concentrated on the lingual finish line and loading point.

MZ세대의 윤리적 소비성향이 사회적 기업의 패션상품 구매의도에 미치는 영향

이상권,정인희,조윤진,Lee, Sang Kwon,Chung, Ihn Hee,Cho, Yun Jin 대한가정학회 2022 Human Ecology Research(HER) Vol.60 No.3

Generation MZ, a generic term for millennials and members of Generation Z, is an innovation-leading consumer group in the current market. This study aimed to examine the impact of the ethical consumption tendencies of millennials and Generation Z on the purchase intention of social enterprise fashion products. Data were collected from Generation MZ (15-40 years old) by quota sampling based on the demographic variables of gender, age, and region, respectively. Eight hundred responses were analyzed by descriptive statistics, factor analysis, t-test, and regressions using SPSS software. Three value factors (egoistic, altruistic, and biospheric) and three ethical consumption tendency factors (resource saving tendency, eco-friendly tendency, and consciousness of corporate responsibility) were determined by factor analyses. The results confirmed that women, who showed a higher ethical consumption tendency in all three factors, were more altruistic and biospheric than men. Furthermore, women portrayed a more favorable attitude and purchase intention towards fashion products of social enterprises. The results demonstrated that members of Generation Z were more altruistic and biospheric than millennials. Additionally, the attitude, purchase intention, and willingness to pay premium prices were higher in Generation Z members. It was confirmed that higher biospheric values correlated with greater values for the three ethical consumption tendency factors. The altruistic value affected both the resource saving tendency and eco-friendly trend, while the egoistic value had an effect on the resource saving tendency. Both attitude and purchase intention were positively influenced by all three of the ethical consumption tendency factors, and affected the willingness to pay premium prices. This was subsequently influenced by the resource saving tendency and consciousness of corporate responsibility. The results of this study contribute to understanding Generation MZ as a consumer group, and the establishment of marketing strategies for fashion companies.

한국산 거머리로부터 항혈전단백질의 검색과 분리.정제

이상권,손정훈,최의성,이상기,Lee, Sang-Kwon,Sohn, Jung-Hoon,Choi, Eui-Sung,Rhee, Sang-Ki 생화학분자생물학회 1993 한국생화학회지 Vol.26 No.3

한국산 거머리인 Hirudo nipponia와 Whitmania sp.의 추출액으로 trypsin, factor Xa, thrombin에 대한 저해활성을 조사하였다. Hirudo nipponia로부터 얻은 추출액은 $20{\mu}g/ml$ 농도에서 이 효소들의 활성을 완전히 저해하는 반면 Whitmania sp.의 추출액은 동일조건하에서 trypsin만을 저해하였다. 이 Hirudo nipponia의 추출물로부터 항thrombin 인자를 분리 정제하기 위하여 anion exchange chromatography, size exclusion chromatography 및 HPLC를 이용하였고 최종적으로 세 종류의 단백질(I-A,I-B,I-C)을 얻었다. 이 중 두 종류 단백질(I-A, I-B)의 아미노말단 서열을 부분적으로 분석한 결과 이 단백질이 주로 산성 아미노산잔기로 구성되어 있으며 heparin cofactor II의 일부분과 유사성이 있음이 발견되었다. Two Korean leech species, Hirudo nipponia and Whitmania sp., were examined for the presence of anticoagulant activity. Crude extracts of the leech head parts were fractionated by ethanol and the anticoagulant activity of these extracts was assessed with respect to the inhibition of thrombin, factor Xa and trypsin using the chromogenic substrate assay. While the extract from Hirudo nipponia inhibited all three serine proteases employed in the assay, that of Whitmania sp. inhibited only trypsin, but not thrombin and factor Xa. Thus, the extract from Hirudo nipponia which shows antithrombin activity was subjected to further purification. Three steps of column chromatography including gel filtration on Superose 12 column, anion exchange on Mono-Q and reverse phase HPLC on C-8 column could resolve 3 antithrombin activity peaks from anti-trypsin and anti-factor Xa activities, indicating the presence of multiple forms of thrombin-specific inhibitors in Hirudo nipponia. N-terminal amino acid sequences of the two of these forms showed a limited degree of homology with a portion of heparin cofactor II.

KDSM(KAIST Distributed Shared Memory) 시스템의 설계 및 구현

이상권,윤희철,이준원,맹승렬,Lee, Sang-Kwon,Yun, Hee-Chul,Lee, Joon-Won,Maeng, Seung-Ryoul 한국정보과학회 2002 정보과학회논문지 : 시스템 및 이론 Vol.29 No.5

본 논문에서는 KDSM(KAIST Distributed Shared Memory) 시스템의 설계 및 구현에 관해서 설명한다. KDSM은 Linux 2.2.13 상에서 실행되는 사용자 수준 라이브러리로 구현되었고, TCP/IP를 기반 통신 구조로 사용한다. KDSM은 페이지 기반 무효화 프로토콜(page-based invalidation protocol)과 다중 쓰기 프로토콜(multiple writer protocol)을 기반으로 하고, HLRC(Home-barred Lazy Release Consistency) 메모리 일관성 모델을 사용한다. KDSM의 성능을 측정하기 위해서 4개의 과학계산용 응용 프로그램을 실행하여 JIAJIA와 성능 비교를 하였다. 그 결과, 2개의 응용은 같은 결과를 냈고, 나머지 2개는 KDSM의 성능이 우수하였다. In this paper, we give a detailed description of KDSM(KAIST Distributed Shared Memory) system. KDSM is implemented as a user-level library running on Linux 2.2.13, and TCP/IP is used for communication. KDSM uses page-based invalidation protocol, multiple-writer protocol, and supports HLRC(Home-based Lazy Release Consistency) memory consistency model. To evaluate performance of KDSM, we executed 4 scientific applications and compared the result to JLAJLA. The results showed that performance of KDSM almost equal to JIAJIA for 2 applications and performance of KDSM is better than JIAJIA for 2 applications.

전기차 능동소리설계 및 제어

이상권(Sang-Kwon Lee),이승민(Seungmin Lee),강인득(Indeuk Kang),이반(Li Pan) 한국소음진동공학회 2015 한국소음진동공학회 학술대회논문집 Vol.2015 No.4

Virtual Motion 을 이용한 자동차용 혼의 동적해석 및 사운드 예측

이상권(Sang-Kwon Lee),배인식(In-Sik Bae),이인석(In-Seok Lee) 한국소음진동공학회 2015 한국소음진동공학회 학술대회논문집 Vol.2015 No.10