보문 : 샌달우드 오일이 탈모 모델 C57BL/6 마우스의모발성장 양상에 미치는 영향

이복순 ( Bok Soon Lee ) 대한미용학회(구 대한미용과학회) 2014 대한미용학회지 Vol.10 No.4

The purpose of the current study was to observe the effects of sandalwood oil (SO) on hair growth status changes asassessed by gross and histological analyses in a C57BL/6 mouse model of alopecia. C57BL/6 mice were randomized intoeither a normal group (N: saline), a positive control group (PC: 3% minoxidil), or an experimental group (E: 3% SO). SO(100 꺤L) was applied topically to the backs of mice once daily for 5 days a week over a 4-week period, and hair growthstatus changes were observed during 16 weeks. Study results revealed that the hair growth effect in the PC and E groupswas significantly greater as compared to the N group. At week 10, mice in the N group showed 13% hair loss as comparedto week 7, while mice in the both the PC and E groups showed 23% hair growth (both p.0.001). Hematoxylin and eosinstains were used for the histological analyses of skin tissues. During 16 weeks, hair follicle (HF) number, HF depth, anddermal thickness in the PC and E groups were significantly increased as compared to the N group. Additionally, thenumbers of mast cells in the PC and E groups at weeks 4 and 16 were significantly decreased as compared to the N group. In conclusion, SO, like 3% minoxidil, promoted hair growth, maintained an anagen phase, and delayed the transition tothe catagen phase in a C57BL/6 mouse model of alopecia.

보문 : C57BL/6 마우스에 샌달우드 오일 도포에 의한 모발성장 관련 효소 활성과 사이토카인의 발현 변화

이복순 ( Bok Soon Lee ),홍성희 ( Sung Hee Hong ),김영철 ( Young Chul Kim ) 대한미용학회(구 대한미용과학회) 2015 대한미용학회지 Vol.11 No.1

This study was conducted to examine changes in hair growth-relevant factors including enzyme activity and cytokine expression induced by topical application of sandalwood oil (SO) to C57BL/6 mice. Mice were randomized into three groups: a normal group (N: saline), a positive control group (PC: 3% minoxidil), and an experimental group (E: 3% SO). SO (100 μL) was applied topically to the backs of mice once a day, five days a week, for four weeks, and changes in hair growth-relevant factors in the skin were observed for 16 weeks. The alkaline phosphatase (ALP) activity in the PC and E groups was significantly higher (p/0.001) than that of the N group. The mRNA expression of hair growth promoting factors including prolactin (PRL) and vascular endothelial growth factor (VEGF) in the PC and E groups increased significantly (p/0.001) as compared to the N group. The mRNA expression of a known hair growth inhibitory factor, transforming growth factor-β1 (TGF-β1), in the PC and E groups was significantly decreased (p/0.001) as compared to the N group. These results suggested that changes in the values of growth-relevant factors including ALP activity and PRL, VEGF, and TGF-β1 mRNA expression were useful as representative markers of the hair growth cycle.

독서교육 프로그램이 초등학생의 공감 능력과 장애아동에 대한 또래 수용태도에 미치는 효과

이복순 ( Bok Soon Lee ),강영하 ( Young Ha Kang ),남윤석 ( Yun Sug Nam ) 한국특수교육문제연구소 2010 특수교육저널 : 이론과 실천 Vol.11 No.2

이 연구는 초등학생이 장애아동의 특성을 이해하고 장애아동과의 상호작용을 돕는 독서교육 프로그램을 구안하여 적용해 보고, 이러한 독서교육 프로그램이 초등학생의 공감 능력과장애아동에 대한 또래 수용태도에 미치는 효과를 밝히기 위한 것이다. 초등학교 5학년 2개반을 실험 및 통제 집단으로 설정하고 사전검사를 실시한 후 실험집단에게는 주 2회씩 총 12회기 동안 프로그램을 적용하였다. 실험집단과 통제집단의 동질성 확보를 위해 사전검사 점수를 t 검증하였으며, 프로그램의 효과를 분석하기 위해 실험집단과 통제집단의 차이에 대해 사전검사를 공변인으로 하고 사후검사를 종속변인으로 하는 공분산분석을 실시하였다. 이 연구에서 얻은 결과는 첫째, 독서교육 프로그램은 초등학생의 공감 능력을 향상시키는데 긍정적인 영향을 끼쳤다. 따라서 독서교육 프로그램은 초등학생의 공감 능력 증진에 효과적인 방법이라고 할 수 있다. 둘째, 독서교육 프로그램은 초등학생의 장애아동에 대한 또래수용태도 변화에 긍정적인 영향을 끼쳤다. 따라서 독서교육 프로그램은 장애아동에 대한 초등학생의 또래 수용태도를 긍정적으로 변화시킬 수 있는 방법이라고 할 수 있다. 결론적으로 독서교육 프로그램이 초등학생의 공감 능력과 장애아동에 대한 또래 수용태도 향상에 긍정적 효과가 있으므로, 통합교육이 이루어지는 교육 현장에서는 아동들에게 공감 능력을 향상시키고 더불어 장애아동에 대한 또래 수용태도를 향상시키기 위한 교육 프로그램으로 적극 활용할 필요가 있겠다. The purpose of this study was to examine the effects of reading education program on the student’s empathic ability and peer receptive attitude to children with disabilities. The subjects of this study is composed of 60 students in 2 classes of the 5th grade at elementary school in Daejeon. After the pre-test has been administered, the reading education program has been treated twice a week, 12 times in total for the experimental group, but not for control group. After the program was finished, the post-test has been administered simultaneously to both groups. The results were as follows: First, the reading education program had a positive effect on the improvement of empathic ability. Second, the reading education program had a positive effect on the improvement of the peer receptive attitude to children with disabilities. In conclusion, the reading education program has the positive effect on the improvement of empathic ability and the peer receptive attitude to children with disabilities.

보문 : 대두 메탄올추출물의 멜라닌 합성 및 티로시나제 활성 증진 효과

이재순 ( Jae Soon Lee ),이복순 ( Bok Soon Lee ),김영철 ( Young Chul Kim ) 대한미용학회(구 대한미용과학회) 2013 대한미용학회지 Vol.9 No.4

The promoting effect of soybean (Glicine maxim) methanol extract (GMME) on the melanogenesis and tyrosinase activity was investigated in melan-a cells, and 3-isobuty-1-methylxanthine (IBMX) was used as positive control (PC). In antioxidative ability analysis, GMME was found to contain 53.7 mg/g of polyphenol and 62.7 mg/g of flavonoid in total. Electron-donating abilities of BHT and GMME at 1,000 μg/mL were 68.4% and 14.4%, respectively. In morphological observation of melan-a cells, as the treatment concentration increased, more dendritic development and melanin accumulation observed. Melanin contents of GMME and IBMX treatment groups were significantly (p/0.001) higher by 51.2% and 31.6% at the concentration of 12.5 μg/mL, respectively, compared to the control group. Intra-cellular tyrosinase activities of GMME and PC treatment groups were significantly (p/0.001) higher by 90.0% and 54.1% at the concentration of 12.5 μg/mL, respectively, compared to the control group. Cell-extracted tyrosinase activities of GMME and PC treatment groups were higher by 12.5% (p/0.01) and 27.2% (p/0.001) at the concentration of 12.5 μg/mL, respectively, compared to the control group. GMME showed a promoting effect on melanogenesis and tyrosinase activity. From these results, it can be speculated that GMME could be applied to improve hair graying.

Melan-a 세포에서 쥐눈이콩과 여우콩 에탄올추출물의 멜라닌생성 관련 유전자 발현 상향 조절 작용

홍성희 ( Seong Hee Hong ),이복순 ( Bok Soon Lee ),김영철 ( Young Chul Kim ) 대한미용학회(구 대한미용과학회) 2016 대한미용학회지 Vol.12 No.2

To determine the molecular mechanisms underlying the melanogenic effects of black soy bean ethanol extracts, including Rhynchosia nulubilis bean ethanol extract (RNBEE), R. nulubilis leaf ethanol extract (RNLEE), R. volubilis bean ethanol extract (RVBEE), and R. volubilis leaf ethanol extract (RVLEE), we evaluated their effects on tyrosinase, TRP-1, TRP- 2, and MITF-M gene expression at mRNA and protein levels. Compared to the control group at 50 μg/mL, tyrosinase and TRP-1 mRNA expression was significantly increased by RNLEE, RVBEE, and RVLEE treatment, whereas RNBEE significantly increased TRP-1 mRNA expression but did not affect tyrosinase mRNA expression. RNLEE significantly increased TRP-2 and MITF-M mRNA expression; RVLEE significantly increased MITF-M mRNA expression. Compared to the control group at 50 μg/mL, RNBEE, RNLEE, and RVLEE significantly increased the protein levels of tyrosinase, TRP-1, TRP-2, and MITF-M. RVBEE significantly increased the protein levels of tyrosinase, TRP-1, and MITF-M, but did not affect that of TRP-2 protein. We conclude that the potential mechanism underlying the melanogenic effects of black soybean ethanol extracts is upregulation of tyrosinase mRNA and protein expression along with an increase in tyrosinase activity. The increase in mRNA and protein levels of TRP-1, TRP-2, or MITF-M by RNEE and RVEE might also be contribute to the increase in melanin synthesis.

보문 : 페파민트 오일의 모발성장 촉진효과 및 항비듬균 활성

오지영 ( Ji Young Oh ),이복순 ( Bok Soon Lee ),김영철 ( Young Chul Kim ) 대한미용학회(구 대한미용과학회) 2014 대한미용학회지 Vol.10 No.4

This study was conducted in order to investigate the hair growth promotion effect and anti-bacterial activity againstPityrosporum ovale of peppermint oil (PEO). Five-week-old male mice were acclimated for one week under 22±1□Croom temperature, 50±5% relative humidity, and 12 hours of a light/dark cycle before experiments commenced. Theanimals were divided into either a normal group (saline, N), a vehicle control group (jojoba oil, VC), a positive controlgroup (3% minoxidil, PC), or an experimental group (3% PEO, E). Test compounds (100 μL) were applied topically oncea day, 6 days a week, for 4 weeks. Hair regrowth was evaluated by gross and histological observation, as well as byimmunolocalization of stem cell factor (SCF) antigen from the skin of the back. Additionally, the antibacterial activityagainst P. ovale of PEO was measured using the Halo and minimal inhibitory concentration (MIC) tests. Study resultsrevealed that topical treatment with 3% PEO promoted hair regrowth faster than with 3% minoxidil. The number of mastcells in the skin of mice in the E and PC groups was lower than in mice in the N and VC groups. Immunolocalization ofSCF antigens was also observed, with high-density staining found in the bulge and sebaceous gland of mice in the E andPC groups. Mild-density staining was also observed in the dermal papilla, sebaceous gland, and epidermis of mice in theN and VC groups. Results from the Halo test demonstrated an inhibition zone against P. ovale of 34.0 mm in 3% PEOtreatment, and the MIC test results revealed that 3% PEO treatment showed stronger antibacterial activity (0.046%) thanthose treated with 3% minoxidil. The absolute weight of the spleen in the PC group was significantly increased, and thenumber of platelets in the PC group was the most significantly decreased. These results indicate that 3% PEO effectivelypromoted hair regrowth in an animal model, and that it also had potent anti-bacterial activity against P. ovale.

여대생들의 헤어 아이롱 웨이브에 따른 형태학적 선호도에 관한 연구 -대구,대전,서울 지역을 중심으로-

박경옥 ( Kyung Ok Park ),이복순 ( Bok Soon Lee ) 대한미용문화예술학회 2013 대한미용문화예술학회지 Vol.2 No.2

The hair beauty culture has been also rapidly and exceptionally changed and the various hair styling accessaries and tools have been marketed to the customers need. Specially, hair iron wave is preferred and used by female college students for time and cost saving. Secondly, the preference for hair iron wave is ranked orderly by short style, medium style, and long style. For short style, thick wave is most preferred by 49.54%, nextly out-curl by 18.35%, C-curl by 14.22%, ripple wave by 10.09%, and strong wave by 7.80%. In medium style, 35.48% for ripple wave which can present purity and most preferred, 26.27% for C-curl, 17.51% for out-curl, 16.59% for thick wave, and 4.15% for string wave. For long style, 51.63% for thick wave, 18.14% for ripple wave, 14.42% for out-curl, 13.95% for C-curl, and 1.86% for strong curl. In this study, the hair style trend, preference of hair iron wave of female uuniversity niversity students by age, present hair style, and living region is surveyed and analyzed, which gives information what they are and ask on hair beauty. The extension of survey region and insufficient the association analysis of demographical and/or socio-economical status with main questionnaires may be necessary in the future study.

기초 : 뇌하수체 선종에서 Met Tyrosine Kinase 수용체의 발현과 의미

박정언 ( Jung Eon Park ),김화정 ( Hwa Jung Kim ),이복순 ( Bok Soon Lee ),이재호 ( Jae Ho Lee ),김세혁 ( Se Hyuk Kim ) 대한뇌종양학회 2008 대한뇌종양학회지 Vol.7 No.2

Objective:Met tyrosine kinase receptor is known as the receptor of hepatocyte growth factor(HGF) and met-HGF signaling has been shown to affect a wide range of biological activities, including angiogenesis, cellular motility, growth, and morphogenetic differentiation. However, little is known about its significance in pituitary adenomas(PAs). The goal of this study is to verify Met-expression and its implication in PAs. Material & Methods:We collected fresh specimens from 29 PAs(11 functioning, 18 nonfunctioning) during surgery. The samples were subjected to RT-PCR using human wild type Met(wtMet) primer to assess Met expression in RNA level, and western blotting to assess its expression in protein level. We analyzed the relationship between Met expression in mRNA or protein level and clinico-radiological factors. Results:wtMet mRNA was expressed in 65.5%(19/29) and Met protein in 17.9%(5/28). Among 29 PAs, the incidence of wtMet mRNA expression was significantly higher in functioning PAs(90.9%) compared to nonfunctioning PAs (50%). And patients` age was significantly younger in wtMet expressed group(mean:42.8 years) compared to nonexpressed group(mean:55.1 years). The expression of wtMet mRNA or Met protein was not correlated with the other clinico-radiological factors including the invasiveness. Conclusion:Our results showed a positive relationship between wild type Met mRNA expression and endocrine activity. We suggest that wild type Met(wtMet) mRNA expression might be one of the markers of functioning adenomas and its clinical implication should be studied further.