자궁근종과 자궁평활근 세포분열에 있어 Interferon-${\alpha}$ 및 basic Fibroblast Growth Factor (bFGF)의 효과

이병석,박정식,김진영,배상욱,박기현,조동제,이국,김재욱,송찬호,Lee, B.S.,Park, J.S.,Kim, J.Y.,Bae, S.W.,Park, K.H.,Cho, D.J.,Lee, K.d,Kim, J.W.,Song, C.H. 대한생식의학회 1997 Clinical and Experimental Reproductive Medicine Vol.24 No.3

Leiomyomas, which are the commonest pelvic tumors in women, are originated from myometrial cells. Although the exact initial pathophysiologic event of the leiomyoma is not known, recent evidences suggested that the effects of sex steroid hormones in the process of tumor growth are mediated by local production of growth factors including epidermal growth factor (EGF), insulin-like growth factor-I (IGF-I) and insulin-like growth factor-II (IGF-II). If we look at the effects of other cytokines, it was suggested that basic fibroblast growth factor (bFGF) may stimulate the proliferation of myometrial and leiomyomas cells. And it was reported that interferon-${\alpha}$ inhibit the action of bFGF. Therefore, we examined the effect of bFGF and interferon-${\alpha}$ on the proliferation of leiomyoma and myometrial cells. bFGF stimulated the myometrial and leiomyoma cells significantly at the concentration of 1ng/ml (p<0.05) and 5ng/ml (p<0.05). However, Interferon-${\alpha}$ inhibited the cell proliferation of myometrial and leiomyoma cells significantly at the concentration of 100U/ml (p<0.05) and 1000U/ml (p<0.05). And the stimulated effects of bFGF with the various concentration on the myometrial and leiomyoma cells ware inhibited by interferon-${\alpha}$ with 100U/ml. Therefore, we concluded that bFGF may stimulate the myometrial and leiomyoma cell proliferation and interferon-${\alpha}$ may inhibit the myometrial and leiomyoma cell proliferation through blocking the effect of basic fibroblast growth factor.

황체화된 인간 과립세포에서 Apoptosis 관련 유전자인 bcl-2와 TRPM-2의 발현

이병석,최은아,장경환,김진영,배상욱,박기현,조동제,이국,김재욱,송찬호,Lee, B.S.,Choi, E.A.,Chang, K.H.,Kim, J.Y.,Bae, S.W.,Park, K.H.,Cho, D.J.,Lee, K.,Kim, J.W.,Song, C.H. 대한생식의학회 1997 Clinical and Experimental Reproductive Medicine Vol.24 No.2

Apoptosis, programmed cell death, is posulated to occur in granulosa cells in ovarian follicular atresia. bcl-2 gene serves as protector from apoptosis and, thus, is associated with increased cell survival. TRPM-2 gene expression has been implicated as a trigger of apoptosis in rat prostate, uterus and mammary gland. Our objective was to determine if bcl-2 and TRPM-2 are expressed in luteinized human GC and, therefore, have regulatory functions for apoptosis in GC. Human GC were obtained via oocyte retrival from the infertile patients stimulated with exogeneous gonadotropins while undergoing IVF. GC were isolated from follicular fluid using Percoll gradient centrifugation. The GC were further purified with anti-CD45 magnetic beads to remove contaminating WBC's. RT-PCR were performed to analyze the mRNA expression of bcl-2 and TRPM-2 in the GC. The PCR primers were designed to amplify a 195 bp fragment of bcl-2 and a 174 bp fragment of TRPM-2. The PCR products were electrophoresed on 4% agarose gel. Three separate experiments indicated that both bcl-2 and TRPM-2 are concurrently expressed in human GC. We cultured granulosa cells with FSH (1 ng/ml) for 1 day to investigate the relative changes of TRPM-2 mRNA level with RNAse protection assay. When we cultured GC with serum free medium for 1 day TRPM-2 mRNA level increased with 1.3 fold, however it was decreased 0.64 fold with FSH. Therefore we conclude that bcl-2 and TRPM-2 are concurrently expressed and that the interaction of their products may be involved in GC apoptosis. And TRPM-2 may be regulated with FSH.

목건초와 농후사료의 급여비율이 면양에 미치는 영향 (2) 목건초와 농후사료의 급여비율이 면양의 반추위내 발효성상 , TURNOVER RATE 및 RUMEN VOLUME 에 미치는 영향

이병석(B . S . Lee),장문백(M . B . Chang),김동식(D . S . Kim),맹원재(W . J . Maeng),정천용(C . Y . Jung) 한국영양사료학회 1995 韓國營養飼料學會誌 Vol.19 No.6

담근먹이 급여가 거세한우의 도체특성에 미치는 효과

홍성구,이병석,강희설,조원모,이종문,백봉현,김내수,송만강 ( S . K . Hong,B . S . Lee,H . S . Kang,W . M . Cho,J . M . Lee,B . H . Baek,N . S . Kim,M . K . Song ) 한국축산학회 1996 한국축산학회지 Vol.38 No.1

Isoflavone 강화 기능성 계란 생산 : 난황내 Genistein 전이 효율 개선

황보종,이병석,이현정,정완태,조성백,홍의철,배해득,장종수,HwangBo, J.,Lee, B.S.,Lee, H.J.,Chung, W.T.,Cho, S.B.,Hong, E.C.,Bae, H.D.,Chang, J.S. 한국가금학회 2006 韓國家禽學會誌 Vol.33 No.3

Genistein의 전이율을 조사하기 위하여 순도 97% 이상의 genistein 250 mg을 펠렛으로 성형한 후 500 mg및 1 g의 용량으로 각각 2, 4개씩 산란계의 어깨 부위 피하에 이식하여 계란의 난황 내에서 검출되는 genistein의 함량을 HPLC 및 HPLC-MS를 이용하여 분석하였다. Genistein 500 mg 처리구에서, 12, 18, 30일에 각각 218, 395 ng/g, 500 ng/g의 genistein 이 전이되어 59일까지 계속적으로 유지되었다. 또한 genistein 1,000 mg 처리구에서는 12일 후에는 240 ng/g, 18일 후에는 406.5 ng/g, 30일 후에는 514 ng/g의 genistein이 전이되어 59일까지 계속적으로 유지되었다. 본 연구를 통하여, 500 ng/g 이상의 genistein이 함유된 계란을 59일 이상 지속적으로 생산할 수 있어 피하이식을 통한 비급이 방식은 기존의 급이 방식에 비해 20배 이상 효과적이었다. This study was carried out to determine whether genistein implants in laying hens could be transferred into their eggs. 250 mg genistein pellet was implanted two or four subcutaneously in the neck of laying hens. The contents of genistein in egg yolk transferred were analyzed with HPLC-MS. In 500 mg genistein pellets, it was detected as 395 ng/egg yolk on the day 18 after implanting and maintained as 546 ng/egg yolk after the day 59. In 1,000 mg, genistein was detected as 240 ng/egg yolk on the day 13, as 514 ng/egg yolk on the day 30 and maintained over 59 days. In conclusion, the direct genistein implants could be more twenty times efficiently transferred to egg yolks than dietary supplement.

산란계에 있어 남은 음식물의 첨가 수준이 생산성과 난질에 미치는 영향

황보종,이성재,이병석,이현정,조성백,김학규,이선업,홍의철,HwangBo, J.,Lee, S.J.,Lee, B.S.,Lee, H.J.,Cho, S.B.,Kim, H.K.,Lee, S.U.,Hong, E.C. 한국가금학회 2006 韓國家禽學會誌 Vol.33 No.3

본 시험은 남은 음식물을 산란계 사료에 1, 3, 5, 10 및 20% 첨가하여, 산란계의 사료 섭취량, 산란율 및 계란 품질에 미치는 영향을 구명하고자 실시하였다. 시험축은 50주령 산란계(White Leghorn종) 162수를 이용하였으며, 처리구는 남은 음식물을 0, 1, 3, 5, 10, 20% 첨가하여 4주간 급여하였다. 시험기간 중 사료 섭취량, 난중은 첨가수준별과 주령별 처리구 사이에 차이가 없었다(p>0.05). 그러나 산란율은 FW 20% 처리구에서 1, 2, 3 주째 산란율의 감소가 있었으나, 4주째 회복하였다(p<0.05). 난각 두께와 난황 계수는 전 처리구에서 차이가 없었으나(p>0.05), 난각 강도는 3주째와 4주째에 10% FW 처리구에서 각각 $4.13kg/cm^2$와 $4.04kg/cm^2$로 나타나 첨가 수준에 따른 차이가 있었다(p<0.05). 호우 유니트와 난황색은 20% FW 처리구에서 4주째에 각각 92.3과 9.4로 남은 음식물 사료의 첨가 수준에 따라 주령별 뚜렷한 차이를 보였다(p<0.05). 본 연구 결과 FW를 20% 이상 첨가시에 산란율이 감소했지만, 5% 첨가시에 산란율이 유지되었으며, 난질이 개선되어 남은 음식물 사료의 영양소 요구량 수준 개선으로 사료 자원으로서 가치가 증진될 것으로 사료된다. This study was carried out to investigate the effect of food-waste(FW) between weeks(0, 1, 2, 3, 4 week) and additive levels(0, 1, 3, 5, 10, 20%) on egg production and egg qualify in laying hens. One hundred sixty two White Leghorn laying hens at fifty weeks of age were used for this work. There was no significant difference on feed intake, egg production, and egg weight among all treatments. But, 20% FW group was decreased at 1, 2, and 3 week on egg production and it was recovered at 4 weeks(p<0.05). There was no difference on the eggshell thickness and egg yolk index among all treatments. However, eggshell breaking strength were $4.13kg/cm^2\;and\;4.04kg/cm^2$ at 3 week and 4 week, individually, and there was difference on eggshell breaking strength(p<0.05). Haugh unit and egg yolk color were high at 20% FW group as 92.3 and 9.4, individually, and there was shown the significant increasing in weeks and additive levels(p<0.05). Conclusionally, 20% FW group was decreased on egg production, while 5% FW group was maintained on egg production, and haugh unit and egg yolk color were cleary developed. Therefore, if the nutrients balance of FW diets were controlled, FW diets will be used with one of the valuable feed sources.

무창계사의 환기 시스템이 케이지 사육 육계의 초기 생산성에 미치는 영향

황보종,홍의철,이병석,배해득,이성재,조성백,김학규,김재황,박병성,송준익,HwangBo J.,Hong E.C.,Lee B.S.,Bae H.D.,Lee S.J.,Cho S.B.,Kim H.K.,Kim J.H.,Park B.S.,Song J.I. 한국가금학회 2006 韓國家禽學會誌 Vol.33 No.1

본 연구는 신축한 무창 실험 계사의 환기 시스템이 육계의 생산성에 미치는 영향을 조사하고자 하였다. 본 실험은 신축 실험계사에서 무창 육계사의 환기 시스템이 케이지 사육 육계의 성장에 미치는 영향을 구명하고자 계사의 내부 환경을 외부와 완전히 차단하여 각종 질병 요인으로부터 계사의 내부 환경을 보호할 수 있는 무창 계사에서 실시하였다. 환경요인 중에서 제일 중요한 온도, 습도, 공기 속도가 육계의 성장에 미치는 영향에 대하여 조사하고자 2주간 실행하였으며, 실험 결과는 다음과 같다. 1. 암모니아 가스 발생량은 케이지 2단(13.7 ppm), 3단(13.5 ppm)에서는 케이지 1단(14.6 ppm)에 비하여 낮게 측정되었다. 오후에 측정한 결과 오전과 비슷한 경향이었다. 이산화탄소는 오전과 오후 케이지간 큰 차이는 없었지만 신선한 공기가 유입되는 4단 높이에서 낮은 경향이었다. 2. 공기 풍속에 있어서는 무창 계사는 상부 0.57 m/sec, 중앙 0.22 m/sec, 하부 0.04 m/sec로 각각 나타나, 계사내 적정 풍속 유지를 위한 덕트 입기구의 천공으로 인하여 계사내 전체 공간에서 공기의 유속 흐름 분포가 고르게 나타났다. 3. 증체량은 시험 전 기간동안 케이지 1에서 케이지 2, 3, 4단에서 보다 높게 나타났지만 유의적인 차이는 없었다(p>0.05). 섭취량은 시험기간 동안 전 처리구에서 유의적인 차이가 없었다(p>0.05). 사료 효율은 증체량과 마찬가지로 케이지 1에서 다른 케이지에 비해 높았으나 유의성은 나타나지 않았다. 이상의 실험 결과를 종합해 볼 때 조사대상이 된 무창 실험 계사의 온도 및 습도 환경 조절이 우수하게 나타났으며 케이지간 공기의 흐름은 원활하였다. This study was conducted to investigate the effect of ventilating system of environmental controlled broiler house on broiler production. Three hundred eighty four broiler chicks (Hubbard, mean live weight of $44.5{\pm}2.0g/bird$) were reared for 2 wk. The results of the present study were summarized as follows : 1. Ammonia gas production was lower in the second and the third floors of cages compared with first floor of cages 13.5 and 13.5 vs 14.6 ppm, respectively). The observations of ammonia production in the morning and afternoon were similar. The production of carbon dioxide was not different between morning and afternoon, but it was tended to decrease in the forth floor of cages due to a fresh air 2. A wind velocity in the enclosed house was similar across lower, middle and upper section (0.57m/sec, 0.22m/sec and 0.04msec, respectively). In order to maintain an optimal air flow velocity in the cages, the duct entrance was punched, and then the air flow was full-round in the overall space in the cage. 3. Daily liveweight gain, feed intakes, and feed efficiency were not significant differences among treatments during whole experimental period (P>0.05). Therefore, the present results showed that temperature, moisture and atmosphere controlling were desirable, and air flew evenly in the cage.

불임환자의 세척정자에 의한 자궁내 인공수정의 임상적 평가

이승헌,윤영욱,이보연,이병석,박기현,황동훈,Lee, S.H.,Yoon, Y.W.,Lee, B.Y.,Lee, B.S.,Park, K.H.,Hwang, D.H. 대한생식의학회 1992 Clinical and Experimental Reproductive Medicine Vol.19 No.1

The sucess of intrauterine inseminations with washed human spermatozoa was evaluated in 92 patients. In according to indication of insemination, intrauterine inseminations of women with hostile cervical mucus yield a 35.3% and 12.8% with male factors and 27.3% with unexplained infertility. In ovulation induction group with variable agents including clomiphen, human menopausal gonadotropin (HMG) and GnRH analog, the pregnancy rate was 27.8% (22/79) and in natural cycle group, 15.4% (2/13). The fetal loss rate in insemination group was 12.5% (3/24). Multiple pregnancies were 7 cases and ovulation induction were performed in 6 cases among them. Intrauterine insemination with washed human spermatozoa therefore represents an effective and safe procedure selected infertile couples.

불임여성에서 자궁근종절제술 후 임신율에 관한 연구

원종건,배상욱,김진영,이지원,이병석,김정수,이경술,박기현,조동제,송찬호,Won, J.G.,Bai, S.W.,Kim, J.Y.,Yi, J.W.,Lee, B.S.,Kim, J.S.,Lee, K.S.,Park, K.H.,Cho, D.J.,Song, C.H. 대한생식의학회 1997 Clinical and Experimental Reproductive Medicine Vol.24 No.2

To evaluate the efficacy of transabdominal myomectomy in the management of infertile patients, and to analyze on the results of abdominal myomectomy in 38 infertile patients with no other detectable cause except myomas were undertaken at the Department of Obstetrics and Gynecology in Yonsei University Hospital from 1990 to 1996. The results are as follows; 1. Average age of patients was 31.1 years. The infertility duration ranged 12 months to 144 months, and average infertility period of patients was 29.4 months. 2. Fourteen of the 38 patients (8 of 23 patients with primary infertility, 6 of 15 patients with secondary infertility) conceived following myomectomy, with a pregnancy rate of 36.8%. 3. Patients with less than 4 years of infertility showed a higher pregnancy rate after myomectomy than those with more than 4 years of infertility (42.4% vs 0%, p<0.05). 4. Patients younger than 35 years showed significantly higher pregnancy rate than those older than 35 years (46.4% vs 9.0%, p<0.05). 5. The removal of a solitary myoma produced a significantly higher pregnancy rate than that of multiple myomas (47.8% vs 20.0%, p<0.05), and the size of the myomas did not influenced the pregnancy rate after myomectomy (p>0.1). 6. The average time period from operation to conception was 12.1 months. Eight of the 14 patients (57.1%) conceived in the first year after operation and 12 patients (85.7%) condeived within two years. In conclusion myomas are a possible cause of infertility and myomectomy can be strongly recommended with good success expectation for the infertile women if uterine myoma be considered as the main cause of infertility. And factors affecting the pregnancy rate after myomectomy in these patients are the age of the patient, the duration of infertiluty, and the number of myoma.

산란계 사료내 CLA 함유 Oil (CLAzen 80) 첨가가 난황내 지방산 조성에 미치는 영향

황보종,장종수,정일병,이병석,김동운,조성백,김희도,배해득,손진혁,홍의철,최낙진,Hwangbo J.,Chang J. S.,Chung I. B.,Lee B. S.,Kim D. U.,Cho S. B.,Kim H. D.,Bae H. D.,Son J. H.,Hong U. C.,Choi N. J. 한국가금학회 2005 韓國家禽學會誌 Vol.32 No.1

본 연구는 oil 형태의 conjugated linoleic acid(CLAzen 80)를 산란계 사료에 수준별로 첨가 급여하였을 때 산란율과 난황내 지방산 조성의 변화를 조사하기 위하여 수행하였다. 59주령의 산란계 72수를 완전임의배치법으로 4개 처리구에 6주간 공시하였다. 처리구는 CLAzen 80를 첨가하지 않은 대조구와 각각 1, 2 및 $3\%$를 첨가구를 두었다. 연구 결과를 살펴보면 산란율은 처리구별 통계적 유의차가 없었지만, 난황내 지방산 조성은 CLAzen 80 첨가에 의하게 크게 영향을 받았다. 난황내 C16:0과 C18:0과 같은 포화 지방산 함량은 CLAzen 80 첨가에 의하여 증가하였으나, 일가불포화지방산인 C18:1 함량은 오히려 감소하였다. 한편, 난황내 C18:2와 C18:3와 같은 다가불포화지방산은 CLAzen 80 급여 2$\~$4주사이에는 모든처리구들에 있어서 그 함량이 일정하게 유지되었다. 그러나, 대조구와 비교하여 CLAzen 80 급여 6주 째에는 난황내 C18:2 함량이 감소하였다. 불포화지방산:포화지방산 비율과 n-6:n-3 불포화지방산 비율은 2$\~$4주 사이에는 처리구별간에 통계적 유의차가 없었고, 6주째 불포화지방산:포화지방산 비율이 CLAzen 80 첨가에 의하여 감소하였다. 한편, 난황내 CLA 함량은 CLAzen 80 첨가수준에 비례하여 증가하였다. 따라서, 산란계 사료 내 CLAzen 80 첨가는 난황내 CLA 함량을 증진시키는 것으로 요약할 수 있다. The objectives of the present study were to investigate the effects of varying levels of dietary oil containing conjugated linoleic acid (CLA) on the egg production and fatty acid composition of egg yolk. Seventy-two 59-wk-old ISA Brown laying hens were randomly allotted to four dietary treatments, each consisting of three replicates with six birds per replicate. There were four treatments that consist of diets containing 0, 1, 2, or $3\%$ commercial CLA-containing oil. Egg production was not significantly different among the dietary treatments at 0, 2, 4, and 6 week. The proportion of saturated fatty acids such as C16:0 and C18:0 in egg yolk were increased, but that of monounsaturated fatty acid C18:1 was decreased by feeding CLA-containing oil supplementation. However, the proportion of polyunsaturated fatty acids such as C18:2 and C18:3 in egg yolk were not different among dietary treatments at 2 and 4 wk of the experiment. At 6 week, the proportion of C18:2 in egg yolk was decreased by feeding CLA-containing oil compared with the control. Polyunsaturated fatty acid:saturated fatty acid (P:S) ratio and n-6:n-3 polyunsaturated fatty acid ratio were similar across the treatments between 2 and 4 week. The P:S ratio was decreased by dietary CLA-containing oil supplementation at 6 week. The proportion of CLA in egg yolk was linearly increased with increasing levels of CLA-containing oil supplementation. In conclusion, dietary supplementation of CLA-containing oil to laying hens increased beneficially increased CLA content in their egg yolk.