Comparative Genomic Hybridization (CGH) of epithelioid sarcoma

이미우 ( Mi Woo Lee ),고경엽 ( Gyung Yub Gong ),지관자 ( Kowan Ja Jee ),최지호 ( Jee Ho Choi ),문기찬 ( Kee Chan Moon ),고재경 ( Jai Kyoung Koh ) 대한피부과학회 2003 초록집 Vol.41 No.10

한국인에서의 피부 림프종의 임상 및 병리조직학적 연구 - 대한피부과학회 피부병리연구분과위원회 공동연구 -

이미우 ( Mi-Woo Lee ),고재경 ( Jai-Kyoung Koh ),권경술 ( Kyung-Sool Kwon ),김낙인 ( Nack-In Kim ),김상원 ( Sang-Won Kim ),김수남 ( Soo-Nam Kim ),김방순 ( Bang-Soon Kim ),김유찬 ( You-Chan Kim ),김종민 ( Jong-Min Kim ),명기범 ( Ki- 대한피부과학회 2003 대한피부과학회지 Vol.41 No.1

Background: The relative frequency and clinicopathologic characteristics of lymphomas vary according to geography and race. Data on the features of cutaneous lymphoma in Korea are limited. Objective . The aim of this study was to document the relative occ

Propionibacterium acnes Peptidoglycan Stimulates Cytokine Production from Monocytes Via Toll-Like Receptor 2

이미우 ( Mi Woo Lee ),( Jenny Kim ) 대한피부과학회 2006 초록집 Vol.44 No.10

Sponsored Lecture 2 : Global aspects of acne treatment: current & future benefits

이미우 ( Mi Woo Lee ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.1

Acne vulgaris is a complex skin disease involving multiple abnormalities of the piloseboceous unit and heyperkeratinization, increasing sebum production, bacterial proliferation and inflammation The pathogenesis of acne is unknown. However several recent studies report an inflammation precedes the occurrence of skin lesion. Besides the currently known TLR, Th17 and inflammasome are also receiving attentions. In case of Th17, the number of Th17 lymphocytes at the acne lesion was increased and P. acnes induced Th17 and Th1 reactions was upregulated in vitro. These reactions were decreased by retinoic acid. Inflammasome is a kind mediator of innate immunity. A study reported that, when monocytes were stimulated by P. acnes, inflammasome such as IL-1 manifests through Nod-like receptor or caspase-1 and IL-1 induces a hyperkeratinization of hair follicle, contributing to comedogenesis of acne. The host response to P. acnes is different between the patient groups with acnes and the normal group without acnes. The monocytes of acne patients showed severe P. acnes-induced immune responses such as IFN-r, IL12P40, IL-8, which may cause more severe inflammatory reaction of acnes. Adapalene 0.1%/ BPO 2.5% (Epiduo®) provides patients with 3 essential actions for acne treatment. It is a unique agent that addresses three of the causative factors of acne as it is anti-comedogenic/ comedolytic, anti-inflammatory and anti-bacterial. This combination is endorsed by the global alliance algorithm which was further endorsed by the AAD in 2013 and more recently by the AAD guideline for pediatric acne for first-line use for the majority of acne patients. However, despite these global recommendations many clinicians still widely use topical antibiotics as first line therapy for acne treatment. They use antibiotics because of their many years of experience and the belief that they work. Physicians believe topical antibiotics are an effective topical therapy. Over time there has been a gradual increase in the rate of antimicrobial resistant P.acnes strains, with one study in Europe reporting up to 63% of P.acnes resistant to both topical erythromycin and clindamycin. Clinicoepidemiological data from Europe suggest that 50% of patients with acne are colonized by clindamycin resistant- P.acnes bacteria; in addition erythromycin resistant P.acnes strains were found in 60% of acne patients and 50% of close contacts. In addition to mounting P.acnes resistance, the use of topical antibiotics has been linked to increased presence of more serious pathogens such as resistant S.aureus in the nose. Margolis et al found that acne patients treated with antibiotics (topical or oral) had 2.15 times greater risk of developing an upper respiratory tract infection compared with acne patients who were not treated with antibiotics. Clearly there are clinical consequences to the misuse of oral and topical antibiotics for acne patients. Oral antibiotics are an important treatment option and are recommended for patients who have moderate to severe form of acne. Unfortunately at this time, there are no other treatment alternatives available to oral antibiotics. This highlights the need for continued investigation for new agents that can address the anti-inflammatory pathway of acne without leading to resistance, such as sub-antimicrobial oral antibiotics. However until new products are available, there is a need to preserve the efficacy of the current agents. One important way to achieve this goal is to ensure that when using oral antibiotics, the treatment therapy is for a limited time and is not used in conjunction with a topical antibiotic. Unlike oral antibiotics, there are highly effective alternatives to topical antibiotics. These alternatives include the use of a topical retinoid and BPO in combination. This combination of Adapalene 0.1%/ BPO 2.5% (Epiduo®) is the first-line recommendation from the Global Alliance and in the recent Acne Guidelines: Acne Treatment Reimbursement Algorithm from the American Academy of Dermatology. It is an effective combination of these two agents that has been proven in over 15 clinical trials with over 5000 patients. In addition, Epiduo® has been proven to effectively inhibited antibiotic-susceptible and antibiotic-resistant P. acnes with eradication rates of 97% for erythromycin resistant; 92% for clindamycin resistant, 92% for tetracycline resistant, 97% for minocycline resistant, and 88% for doxycycline resistant at 4 weeks. Adapalene 0.1%/ BPO 2.5% (Epiduo®) is the antibiotic free alternative that clinicians should consider first when prescribing a topical treatment for acne Recently an ex vivo immunohistochemistry study, it’s investigated the potential synergistic modulator effect of adapalene associated with BPO on keratinocytes proliferation / differentiation and innate immunity in inflammatory acne lesions. It demonstrated that proliferation (Ki-67), adhesion / differentiation (integrin a2, a3 and a6) and innate immunity (TLR-2, b-defensin 4, IL-8) markers areoverexpressed in inflammatory acne skin compared with uninvolved acne skin. Association of adapalene and BPO significantly decreased expression of Ki67, a2 and a6 integrins, TLR-2, b-defensin 4 and IL-8 in inflammatory acne skin, whereas single treatments with adapalene or BPO alone were less effective. These results contribute to explain the comedolytic and anti-inflammatory activities of this combined therapy observed in recent clinical trials.1 Additionally adapalene 0.1%/ BPO 2.5% (Epiduo®) and doxycycline is reported that they aren’t inferior to efficacy compare with oral isotrentinoin in severe acne with nodular. Hence it could be alternative treatment option targeted on contraction of oral isotrentinoin and side effect.

소아 전신성 홍반성 루푸스의 피부 소견에 대한 연구

이미우(Mi Woo Lee),최지호(Jee Ho Choi),성경제(Kyung Jeh Sung),문기찬(Kee Chan Moon),고재경(Jai Kyoung Koh) 대한피부과학회 2000 대한피부과학회지 Vol.38 No.5

N/A Background:SLE is a multisystem disease. Skin manifestations are some of the most common symptoms. Objective:Our purpose was to study in detail the cutaneous lesions in childhood SLE. Methods:We assessed the mucocutaneous signs in 19 children with SLE seen during a 10 years period. Results:1. The female:male ratio was 3.8:1. 2. Cutaneous manifestation(79%) was the third common finding, next to ANA positivity and fever. 3. The skin changes noted were malar rash(58%), oral ulcer(32%), alopecia(26%), photosensitivity(21%). 4.There was no case of subacute cutaneous LE and bullous LE. Conclusion:Skin changes play a prominent part in SLE and may provide helpful diagnostic information. In this study, malar rash was the most common manifestation. (Korean J Dermatol 2000;38(5):607~611)

Erythematous Mass on the Pubic Area

이미우 ( Mi Woo Lee ),노재윤 ( Jae Yoon Ro ),최지호 ( Jee Ho Choi ),문기찬 ( Kee Chan Moon ),고재경 ( Jai Kyoung Koh ) 대한피부과학회 2003 초록집 Vol.41 No.10

Pathology ABC 3 (P 3) : Special staining for dermatopathology beginners

이미우 ( Mi Woo Lee ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.1

contents as glycogen, mucosubstances, melanin, iron, calcium, collagen, fat, neural elements, microorganisms, and others. A comprehensive discussion of the specific reactions involved, and all the circumstances in which they might be applied is covered in the lecture. General principles and pertinent brief summaries of selected histochemical methods are provided in our discussion.

Symposium 3-4 (SYP 3-4) : Management of facial erythema of rosacea

이미우 ( Mi Woo Lee ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.2

Tetracyclines have the anti-inflammatory effects, such as inhibition of metalloproteinases, inhibition of collagenase (MMP1, MMP8, MMP13), inhibition of gelatinase (MMP-2, MMP-9), increase of TIMP, anti-angiogenesis, decreased expression of proinflammatory cytokines, inhibition of neutrophil chemotaxis and ROS, and decreased expression of nitric oxide. Recently a study reported doxycycline indirectly inhibits proteolytic activation of tryptic kallikrein-related peptidase and activation of cathelicidin. Topical calcineurin inhibitors selectively target T cells and mast cell, preventing the production of cytokine and inflammatory mediators. Side effects of TCI are immediate aggravation (1-3d), burning sensation, and calcineurin inhibitor induced rosacea. Azelaic acid showed decreased expression of TLR2, cathelicidin, and KLK-5 in vitro murine skin. Flushing can be induced by humural or neural stimuli, therefore a- adrenergic receptor agonist, such as oral clonidine and topical brimonidine tartrate and oxymetazoline can be used for treatment of rosacea. The use of β- adrenergic receptor blocker, such as oral nadolol, propranolol hydrochloride, and carvedilol were reported.

경화부종의 임상 및 병리조직학적 연구

이미우(Mi Woo Lee),최지호(Jee Ho Choi),성경제(Kyung Jeh Sung),문기찬(Kee Chan Moon),고재경(Jai Kyoung Koh) 대한피부과학회 2002 大韓皮膚科學會誌 Vol.40 No.2

N/A Background : Scleredema is a rare connective disorder with unknown etiology. There were no comprehensive studies about the clinical and histopathological features of scleredema in Korea. Objective : Our purpose was to find the clinical and pathological features of scleredema. Methods : The 31 patients with scleredema at our institution over ten years were retrieved. We reviewed medical records, clinical photographs, and histopathology slides of these patients, retrospectively. Results 1. The male to female ratio was 23:8 (2.9:1). 2. Most of the patients had the insidious onset and chronic localized lesion on the nape and upper back. 3. In sixteen patients (16/31, 51.6%), the diabetes mellitus was accompanied. In these patients, the male predominance (14/16, 87.5%) was significant. 4. The positive staining was in 75%(15/20) of the tissues with alcian blue or mucicarmine staining. 5. In four patients, localized electron beam therapy markedly improved the skin lesion without serious adverse effects. Conclusion : A preceding respiratory infection was uncommon. Almost all patients had insidious onset and chronic course. Diabetes mellitus was commonly accompanied, especially in male patients. Electron beam therapy appears to be effective in the treatment of scleredema.

막낭성 변화를 보이는 지방층염에 대한 임상 및 병리조직학적 연구

이미우(Mi Woo Lee),최지호(Jee Ho Choi),성경제(Kyung Jeh Sung),문기찬(Kee Chan Moon),고재경(Jai Kyoung Koh) 대한피부과학회 1999 大韓皮膚科學會誌 Vol.37 No.10

N/A Background : Membranocystic change represents a distinctive from of pathology in adipose tissue. It has been oberved in a variety of inflammatory and noninflammatory dermatoses. Objective : Our purpose was to find the clinical and histopathological features of membranocystic change in the panniculitis and we attempted to review the pathogenesis. Methods : We reviewed medical records, clinical photographs, and histopatoligic finding of 14 patients having membranocystic changes in biopsy specimen. Results : ① There was a female predominance (79%). ② The most common primary lesions were the subcutaneous nodules and the most predilection sites were the lower legs. ③ The underlying diseases were varible such as erythema nodosum, lipoatrophy, sparganosis, sportrichosis, accelerated nodulosis, necrobiosis lipoidica, periarteritis nodosa, artery stenosis, dermatomyositis, lupus erythematosus profundus, oil granuloma and nodulocystic fat necrosis. ④ The membranes of cyst were positive with periodic acid-Schiff stain with diastase and sudan black B. Fluorescence examination gave a yellow autofluorescence. These findings indicated the lining materials were ceroid. Conclusion : Membranocystic change appears to be entirely nonspecific and may be seen in many types of the subcutaneous inflammatory process. Although ischemic insult has received the most attention in dermatololgic literature as the primary cause, it is likely that this change results from various insults to adipose tissue, including infectious, autoimmune, and physical processes. (Korean J Dermatol 1999;37(10) : 1407∼1412)