연구전산망(KREONet) 구축현황 및 고도화 발전방향

이명선,Lee, Myeong-Seon 한국과학기술정보연구원 2001 지식정보인프라 Vol.4 No.-

연구전산망은 연구개발에 필요한 자료의 교환 결과로써 생성된 지식과 정보를 저장, 가공, 확산시키기 위한 정보망이라고 할 수 있으며, 연구전산망 사업은 국내 연구개발 정보를 필요로 하는 수요자에게 효과적으로 제공하고자 하는 목적에서 추진되었다.

유기 게르마늄 농축수로 재배한 농산물의 항염 및 함암효과

이명선,Lee, Myeong-Seon 한국식생활문화학회 2021 韓國食生活文化學會誌 Vol.36 No.1

The study was conducted to identify the anti-inflammatory and anti-cancer effects in sprouts of mouse-eyed bean (Rhynchosia nulubilis), ginseng (Panax ginseng), perilla (Perilla frutescens), broccoli (Brassica oleracea var. italica), and lettuce (Lactuca sativa) grown with organic germanium concentrate. Western blot analysis was performed to assess the anti-inflammatory activity of the extract. All extracts exhibited noticeable anti-oxidant activity, indicating a significant correlation between the germanium content and anti-oxidant activity (p<0.05). In particular, rat-eyed bean sprouts with the highest germanium content showed significant anti-inflammatory activity (p<0.05) by significantly inhibiting the expression of the inflammatory complexes, NLRP3, cytokines IL-1β and caspase-1. Ginseng and broccoli sprouts showed strong anti-cancer properties and had high anti-oxidant effects (p<0.001). Germanium-concentrated water allows the mass production of agricultural products containing high concentrations of organic germanium. Agricultural produce grown with germanium concentrate add organic germanium to various physiological active ingredients, increasing the anti-oxidant and anti-cancer effects. These results strongly suggest that agricultural products containing high germanium concentrations can be used as novel health supplements to improve health.

인체 자궁암세포에서 cisplatin과 산삼배양근추출물에 의한 apoptosis유도

이명선,Lee, Myeong-Seon 한국현미경학회 2010 Applied microscopy Vol.40 No.3

인체 자궁암 세포 HeLa에 항암제 cisplatin (CDDP)과 산삼배양근 추출물(ETCMG)을 투여하여 세포 성장률, 형태, 전기영동에 의한 DNA fragmentation, 세포주기 분석을 통하여 apoptosis 유도를 확인하였다. ETCMG 2, 4, 6mg/mL, CDDP $4{\mu}g/mL$의 농도로 24시간 투여한 후 세포의 성장에 미치는 영향을 분석하고, ETCMG를 항암제로서 효과가 입증되어있는 CDDP와 복합투여하여 비교한 결과 apoptosis비율은 대조군에 비하여 ETCMG의 농도가 증가할수록 농도에 비례하여 현저히 증가하였고 (p<0.05), CDDP와 ETCMG를 복합투여한 경우에 ETCMG를 단독으로 투여한 경우 보다 apoptosis비율이 매우 높은것으로 나타났다(p<0.05). 세포의 형태를 도립현미경과 투과전자현미경으로 관찰한 결과, 대조군은 세포의 정상적인 형태를 유지하고 있는 반면에 CDDP와 ETCMG를 각각 처리한 암세포는 세포의 성장이 현저히 억제되었고, 염색질의 응축과 apoptotic body가 관찰되었다. 세포의 성장억제가 apoptosis에 의한 것인지를 확인하고자 DNA를 분리하여 전기영동한 결과, HeLa 세포에서 ETCMG의 농도가 증가할수록 ladder가 뚜렷이 관찰되었고, CDDP를 복합 처리한 것 역시 ETCMG의 농도에 비례하여 ladder가 선명하게 나타났다. Flow cytometry (FC)에 의한 세포주기 분석 결과, ETCMG를 농도별로 처리한 경우에 apoptosis를 나타내는 Sub-$G_1$기의 양이 농도에 비례하여 증가하였고, 항암제인 CDDP와 복합 투여한 경우에 Sub-$G_1$기 DNA양이 눈에 띠게 증가한 것으로 나타났다. 이상의 실험 결과를 종합하면, ETCMG는 인체 자궁암에서 항암효과를 가지고 있으며, 항암제 CDDP의 단독 투여보다는 ETCMG와 함께 사용하는 경우에 암 치료제로서의 상승효과가 있는 것으로 사료된다. Mountain ginseng is a perennial crop rarely found in the deep mountains of Korea. The medicinal effect of the mountain ginseng is well known as a panacea in traditional Chinese medicine for a long time. But scientific studies to elucidate the medicinal effect of the mountain ginseng have never been made on account of lack of sample. Recently an improved method of adventitious root culture system through the use of bioreactor has been developed in Panax ginseng that seems to be a reliable way of commercialization of root derived secondary metabolites. This experiment was conducted to evaluated chemotherapeutic effect against human cervical cancer cells by cisplatin (CDDP) and extract of tissue cultured mountain ginseng (ETCMG). CDDP and ETCMG-induced apoptotic cell death in human cervical cancer cell line, HeLa was confirmed by the analysis of cell growth, morphological changes, DNA fragmentation, flow cytometry showed that ETCMG is an inducer of apoptosis and synergizes with CDDP. These results suggest that ETCMG present evidence of anticancer effect and could have a possibly natural therapeutic potential in cervical cancer patients.

가성소다, 액체암모니아 처리한 텐셀의 염색성

이명선,배소영,Lee, Myeong-Seon,Bae, So-Yeong 한국섬유공학회 1997 한국섬유공학회지 Vol.34 No.8

The purified tencel fabrics were treated with NaOH, liquid ammonia (NH$_3$), NaOH and subsequently NH$_3$, and also market product tencel (MPT) treated arith NH$_3$. Finally the pretreated tencel fabrics were processed with enzyme (cellulase). The crystalline structure, SEM, moisture regain, water absorbency, tensile strength, and dyeing behavior of the specimens were studied. The degree of crystallinities decreased in the order of MPT-NH$_3$ > NaOH/MH$_3$ ) MH$_3$ > NaOH > MPT > untreated tencel. However moisture regain and water absorbency of the NH$_3$-treated tencel were lower than those of the untreated because of swelling characteristic of MH$_3$. The NH$_3$ treated-tencel fabric increased in the dry strength compared to untreated tencel. The increase in rate of dyeing was in the order of NaOH > NaOH > NH$_3$ > MPT-NH$_3$ > MPT > untreated tencel fabrics. The equilibrium exhaustion appeared In the order of NaOH > NaOH/NH$_3$ > MPT-NH$_3$ > untreated > MPT The fiber surface of the NH$_3$-pretreated tencel fabrics was remarkably fibrillated by cellulase treatment. The weight loss by cellulase treatment increased in the order of NaOH/NH$_3$> NH > NaOH-treated tencel fabrics and equilibrium exhaustion decreased by cellulase treatment.

신생아 집중치료실 신생아에게 적용된 간호진단분석: NANDA 간호진단분류체계 이용

이명선(Lee Myeong-Seon),최순희(Choi Soon-Hee) 전남대학교 간호과학연구소 2016 Nursing and Health Issues(NHI) Vol.21 No.1

I. 서 론 Ⅱ. 연구 방법 Ⅲ. 연구 결과 Ⅳ. 논 의 Ⅴ. 결론 및 제언

Effects of Antioxidant and Anticancer by Phytoestrogen in Human Breast Cancer Cells

Myeong-Seon Lee(이명선),Kwang-Seok Oh(오광석) 한국엔터테인먼트산업학회 2017 한국엔터테인먼트산업학회논문지 Vol.11 No.2

세계적으로 유방암은 여성에서 가장 많이 진단되는 암으로 외과적 수술, 표적, 항암치료, 호르몬요법, 방사선치료와 이들의 복합치료 등 새로운 치료방법의 개발에도 불구하고 여전히 재발이나 전이, 약물의 저항성과 같은 문제를 가지고 있다. 암의 원인중에 80-90%가 환경적 요인이며 이중에 50-70%가 음식물섭취와 관련이 있다. 최근 야채와 과일섭취가 암이나 심혈관질환 및 만성질환 예방과 관련이 있다고 알려지면서 이에 대한 관심이 높아지고 있다. 역학적 연구는 식물호르몬을 포함하여 야채와 과일에 들어있는 파이토케미컬이 건강과 밀접한 관계가 있음을 시사하고 있다. 본 연구는 식물성 호르몬으로 알려져 있는 콩과 홍삼, 칡(갈근)이 유방암세포에 미치는 영향을 알아보고자 항산화 및 항암효과를 분석하였다. 3가지 종류의 콩, DPPH분석결과 서리태, 노란콩, 쥐눈이콩과 홍삼, 갈근 모두에서 항산화효과를 나타내었으며 FACS 분석결과 bisphenol A는 유방암세포의 성장을 촉진하였고, 홍삼과 갈근추출물은 유방암세포에서 각각 99.3±6.38과 98.9±4.87의 사멸율을 나타내어 bisphenol A에 의한 암세포의 증식을 유의적으로 억제하는 것으로 나타났다(p<0.05>. 이러한 결과는 홍삼과 갈근이 유방암 치료제 개발을 주도해 나가는 새로운 성분을 제공할 것으로 기대된다. Breast cancer is the most common form of cancer diagnosed in women worldwide. Despite the development of newer diagnostic methods, selective as well as targeted chemotherapies and their combinations, surgery, hormonal therapy, radiotherapy, breast cancer recurrence, metastasis and drug resistance are still the major problems for breast cancer. There are growing interests in the health benefits associated with consumption of fruits and vegetables, especially for the prevention of cancer, cardiovascular or other chronic diseases. Antioxidants and phytochemicals in vegetables are known to provide health benefits. Strategies that enhance these properties are expected to increase the nutritional values of vegetables. Epidemiological studies and clinical trials suggest that these health benefits are strongly associated with phytochemicals found in fruits and vegetables including phytoestrogen. However the (anti)estrogenic properties of phytoestrogens have also raised concerns since they might act as endocrine disruptors, indicating a potential to cause adverse health effects. The objective of this research is to assess the effects of antioxidant and anticancer by phytoestrogen as three different kinds of soybean, red ginseng(RG), and kudzu. Extract of these was determined to scavenge free radicals. All five extracts showed antioxidant activity at 50mg/ml of concentration. In flow cytometry assay, RG and kudzu exhibited the highest apoptotic activity(IC50: 99.3 ±6.38 and 98.9 ±4.87) in MDA-MB-231 respectively. Bisphenol A(BA) alone promoted the cell viability of MDA-MB-231 cells. On the other hand, RG and kudzu significantly suppressed BA-induced cell growth. Statistical analyses revealed RG and kudzu exhibited significantly higher (P < 0.05) cytotoxicity in MDA-MB-231 cells. These findings indicate that RG and kudzu may serve as novel therapeutic agents for breast cancer treatment and future leads for drug development.

Effects of Kadzu and Moringa on Hormone-Sensitive Human Prostate Cancer

Myeong-Seon Lee(이명선) 한국엔터테인먼트산업학회 2019 한국엔터테인먼트산업학회논문지 Vol.13 No.2

본 연구는 식물성 모링가와 칡이 호르몬-민감성 전립선암에 어떤 효능을 나타내는지를 확인하고자 전립선 특이항원 및 항산화 활성과 항암활성을 분석하였다. 각각 에탄올로 추출하여 control, 0.1, 1.0, 10.0, 50.0㎎/ml의 농도에서 DPPH 분석한 결과 칡은 50㎎/ml에서 42.1%, 과라나는 73.9%의 소거율을 보여 유의적인 항산화 활성이 있음을 확인하였다(p<0.05). 호르몬에 민감한 인체 전립선암 세포주인 LNCaP에서 항암효과를 분석한 결과, 10㎎/ml의 농도에서 모링가는 22±4.24%, 칡은 39.9±3.53%의 성장률을 나타냄으로써 분석한 두 가지 추출물 모두에서 뚜렷한 항암 효과가 있었고, 모링가 추출물이 칡 추출물보다 다소 높은 항산화 활성과 항암효과를 나타내었다. 이와 같은 결과는 천연 식물성 성분인 칡과 모링가가 호르몬에 민감한 전립선암의 예방과 치료에 새로운 항암제로서의 기초자료를 제공하는 것이며, 향후 이들 추출물 중에 어떤 특정성분이 이러한 역할을 하는지와 이에 대한 작용기작 및 안전성연구가 뒷받침되어야 할 것으로 사료된다. Prostate cancer(PrCa) is a leading cause of cancer-related death in man. Medicinal plants are exploited for many drugs to treat various ailments. The drugs derived from the plants promote health, augmented the resistance of the body against disease. Pueraia lobata(wild) Ohwi(P. Lobata), kudzu, which is a twining perennial woody herb native to China, Korea, Japan, India, and the United States. Plants such as Moringa oleifera, have hypoglycemic properties and other beneficial properties. The objective of the study was to analyze the effects of kadzu and moringa, natural plant products on antioxidant activity and proliferation of the hormone-sensitive prostate cancer LNCaP cells. MTT assay, flow cytometry analysis were employed to investigate the anticancer mechanism and DPPH assay was determined to the antioxidant activity to scavenge free radicals in extract of these. All two extracts showed significantly antioxidant activity at 10 and 50㎎/ml of concentration. kadzu and moringa reduced LNCaP cell viability in a dose dependent manner. Specially moringa extract was more potent cytotoxic than kadzu extract. Statistical analyses revealed kadzu and moringa exhibited significantly higher (P < 0.05) cytotoxicity and antioxidant activity in LNCaP. The finding of this study provides a scientific basis for using kadzu and moringa in future development of chemotherapeutic drugs against hormone-sensitive prostate cancer.

동애등에와 게르마늄수를 활용한 라이프 케어 기능성 건강소재 개발

이명선(Myeong-Seon Lee),오광석(Kwang-Seok Oh),오윤진(Yun-Jin Oh) 한국엔터테인먼트산업학회 2018 한국엔터테인먼트산업학회논문지 Vol.12 No.2

동애등에는 음식물 쓰레기를 처리하는 환경정화용곤충으로 알려져 있으나 양질의 아미노산, 지방산을 함유하고 있고, 항생물질을 분비하여 화장품, 의약품, 건강 기능식품의 소재로서 산업적으로 이용될 가능성을 보이고 있다. 본 연구에서 게르마늄수로 수경재배한 미나리를 사료에 배합하여 사육한 동애등에가게르마늄을 함유하는 유충과 분변토를 생산하는데 성공하였다. 유충과 분변토의 건조분말에서 각각 0.42ppm, 5.84ppm의 게르마늄이 검출되었고, 일반유충과 분변토는 각각 0.01ppm, 0.04ppm 의 게르마늄을 함유하고 있었다. 이들의 추출물에서 DPPH분석 및 암세포의 성장분석 결과 일반유충에 비하여 게르마늄을 다량 함유하고 있는 유충에서 탁월한 항산화 및 항암 효과를 나타내었다(p<0.05). 현재 분변토는 비료로 시판되고 있다. 본 연구에서 생산한 다량의 게르마늄을 함유하고 있는 분변토는 동애등애 유충의 부산물로서 기능성 버섯재배, 과채류 재배 등 에 차별화된 비료로 유용하게 이용할 수 있어서 부가가치를 창출하는 데 크게 기여할 것으로 사료된다. 이러한 결과는 동애등에 유충이 화장품, 의약품, 건강기능식품과 같은 라이프 케어 기능성 건강 소재로 활용 가능성이 있음을 강력하게 시사하는 것이다. The black soldier fly(BSF), Hermetia illucens, is a generalist detritivore that is usually exploited for recycling environmental wastes. BSF larvae are converters of organic waste into edible biomass, of which the composition may depend on the substrate. These insects can also be used to produce antibiotics for medical and cosmetical and functional food applications. In this study, it was the first success to produce BSF larvae containing high dose of germanium utilizing dropwort which is cultivated from germanium concentrated water in the world. The growth inhibitory effect of BSF larvae and feces soil extract containing germanium was determined in cultured prostate cancer cell line LNCaP. The growth of LNCaP was significantly inhibited by the addition of 25-100mg/ml of BST larva powder extract(LPE) and feces soil extract(FSE) containing germanium. Its germanium contents in LPE and FSE were 0.42ppm and 5.84ppm, respectively. LPE displayed more potent anticancer activity than PSE. Also, LPE showed a little more potent antioxidative activity than FSE by DPPH assay. FSE containing high dose of germanium can utilize as valuable feedstuff in functional food production. These results suggest that the effectiveness of antioxidant and anticancer in BSH larvae containing germanium may be useful as materials of cosmeceutical and therapeutical agent and functional health food in life care.

곤충자원을 활용한 라이프케어 소재 개발

이명선(Lee-Myeong Seon),오광석(Oh-Kwang Seok) 한국엔터테인먼트산업학회 2017 한국엔터테인먼트산업학회 학술대회 논문집 Vol.2017 No.11

증기발생기 세관파단사고 모의 시험설비 내 에어로졸 손실량 예측을 위한 PLC 모델링

이명선(Myeong-Seon Lee),이효진(Hyo-Jin Lee),하광순(Kwang-Soon Ha),김성일(Sung-Il Kim) 대한설비공학회 2022 설비공학 논문집 Vol.34 No.2

The amount of aerosol retention in the pipe of the test facility was analytically evaluated in the steam generator tube rupture experimental facility installed in the Korea Atomic Energy Research Institute (KAERI). The amount of aerosol removal in the pipe can be varied greatly depending on the thermo-hydraulic conditions (gas velocity, pressure, temperature, etc.) of the gas containing the aerosol. It is also known that the geometry of the pipes, such as length, curvature, and diameter can be important factors in determining the amount of aerosol retention inside the pipe. Thus, sensitivity calculations were performed on various uncertain variables to identify a decontamination factor of the test facility. In this study, the PLC (Particle Loss Calculator) program was used to evaluate the decontamination factor of aerosol inside the pipes. The calculation results were also compared to the experimental data obtained from the KAERI. It was found that the analysis results underestimate the decontamination factor due to the limitation of the models considered in the PLC program.