兩義供辰丹을 倂用한 한방치료가 重症度의 통증 환자에 미치는 진통 효과 : 후향적 연구

이동혁,이영은,이보윤,김연진,조승연,박성욱,정우상,문상관,고창남,조기호,박정미,Lee, Dong-hyuk,Lee, Young-eun,Lee, Bo-yun,Kim, Yeon-jin,Cho, Seung-yeon,Park, Seong-uk,Jung, Woo-sang,Moon, Sang-kwan,Ko, Chang-nam,Cho, Ki-ho,Park, Jung-mi 대한한방내과학회 2015 大韓韓方內科學會誌 Vol.36 No.3

Objectives: The aim of this study was to investigate the analgesic effect of Yang Eui Gongjin-dan (YEGJD), a traditional Korean medicine, on severe pain or numbness, including acute and chronic non-cancer pain. Methods: Records of patients visiting the Department of Traditional Korean Internal Medicine, Stroke and Neurological Disorders Center, from March 2011 to January 2015, were searched. Their chief complaint was severe numbness or pain (NRS≥7). We compared the NRS of pain pre- and post-YEGJD treatment in 34 patients; and analyzed changes in pain severity pre- and post-YEGJD treatment in 17 chronic non-cancer pain patients. We also investigated other accompanying symptoms, such as insomnia, anorexia, dyspepsia, fatigue, coldness, and excessive sweating, related to the deficiency state (虛症) in traditional Korean medicine. Results: The average pain NRS for the 34 patients significantly decreased from 8.04±1.08 to 3.75±2.54 after YEGJD treatment. The average pain NRS score in chronic non-cancer pain patients also significantly declined from 7.91±0.97 to 3.29±2.29. Conclusions: The traditional Korean medicine Yang Eui Gongjin-dan has an analgesic effect and is useful for treating severe pain or numbness. We recommend a randomized controlled trial using objective assessment scales and a large patient sample to further test the effects of Yang Eui Gongjin-dan.

양측성 외전신경 마비 환자 치험 1례

이동혁,이영은,이보윤,두경희,김수경,조승연,박성욱,박정미,고창남,Lee, Dong Hyuk,Lee, Young Eun,Lee, Bo Yun,Doo, Kyung Hee,Kim, Soo Kyung,Cho, Seung Yeon,Park, Seong Uk,Park, Jung Mi,Ko, Chang Nam 대한중풍순환신경학회 2014 대한중풍.순환신경학회지 Vol.15 No.1

■ Objectives The purpose of this case report is to show an effect of korean medicine for patient with bilateral abducens nerve palsy. ■ Methods This patient was 58 year-old man who was diagnosed with bilateral abducens nerve palsy. The patient had Diabetes Mellitus, hypertension, benign prostatic hyperplasia and dyslipidemia as underlying diseases. The patient had symptoms of limited eye movement to lateral, diplopia, dizziness and mild headache. He was treated by korean medicine, containing electroacupuncture, pharmacoacupuncture, moxibustion and herb medicines during 13 days of hospitalization and following outpatient department. His eye movement was evaluated by the method of Scott and kraft and discomfort from his symptoms was scored by the Numerous Rating Scale. And other general conditions were measured. ■ Results After that period, patient's abduction deficit was improved and subjective discomfort from his symptoms was getting better. His other conditions were also better, compared with his admission. ■ Conclusion This study suggests that Korean medicine could have a therapeutic effect for bilateral abducens nerve palsy. So, It could help to relieve patient's symptoms and make general conditions better.

Bovine Herpesvirus Type 1 정량 검출을 위한 Real-Time PCR

이동혁,정효선,이정희,김태은,이정숙,김인섭,Lee, Dong-Hyuck,Jeong, Hyo-Sun,Lee, Jung-Hee,Kim, Tae-Eun,Lee, Jung-Suk,Kim, In-Seop 한국미생물학회 2008 미생물학회지 Vol.44 No.1

소의 혈액, 세포, 조직, 기관 등은 생물의약품과 조직공학제제, 세포치료제의 원료로 널리 사용되고 있다. 소유래 물질을 원료로 사용한 제제의 경우 소유래 원료 물질에 다양한 바이러스가 오염된 사례가 있기 때문에 바이러스 안전성 검증이 필수적이다. Bovine herpesvirus type 1 (BHV-1)은 소에게 가장 흔하게 감염되는 바이러스중의 하나이다. 소유래 물질을 원료로 하는 생물의약품, 조직공학제제, 세포치료제 등에서 BHV-1안전성을 확보하기 위해, 원료물질, 제조공정, 완제품에서 BHV-1을 정량적으로 검출하고, 제조공정에서 BHV-1 제거 검증을 위한 시험법으로 활용이 가능한 BHV-1 real-time PCR 시험법을 확립하였다. BHV-1에 특이적인 primer를 선별하였으며, 형광염료 SYBR Green I을 사용하여 BHV-1 DNA 정량 검출 시험법을 최적화하였다. 세포배양법에 의한 감염역가와 비교한 결과 real-time PCR 민감도는 $2\;TCID_{50}/ml$이었다. 확립된 시험법의 신뢰성(reliability)을 보증하기 위해 시험법 검증을 실시한 결과 특이성(specificity)과 재현성(reproducibility)이 우수함을 확인하였다. 확립된 real-time PCR을 생물의약품 제조공정 검증에 적용할 수 있는지 확인하기 위하여 인위적으로 BHV-1을 오염시킨 Chinese hamster ovary (CHO) 세포주와 소유래 콜라겐에서 BHV-1 검출 시험을 실시하였다. BHV-1을 감염시킨 CHO 세포에서 세포변병효과를 관찰할 수 없었지만, 세포와 세포배양 상청액에서 BHV-1을 정량적으로 검출할 수 있었다. 소유래 콜라겐에서도 $10\;TCID_{50}/ml$까지 정량적으로 검출할 수 있었다. 위와 같은 결과에서 확립된 BHV-1 real-time PCR 시험법은 생물의약품 안전성 보증을 위한 세포주 검증, 생물의약품생산공정 검증, 바이러스 제거 공정 검증 등에서 감염역가 시험법과 같은 생물학적 시험법을 대신할 수 있는 신속하고, 특이성과 민감성이 우수한 시험법임을 확인하였다. Bovine blood, cell, tissue, and organ are used as raw materials for manufacturing biopharmaceuticals, tissue engineered products, and cell therapy. Manufacturing processes for the biologicals using bovine materials have the risk of viral contamination. Therefore viral validation is, essential in ensuring the safety of the products. Bovine herpesvirus type 1 (BHV-1) is the most common bovine pathogen found in bovine blood, cell, tissue, and organ. In order to establish the validation system for the BHV-1 safety of the products, a real-time PCR method was developed for quantitative detection of BHV-1 in raw materials, manufacturing processes, and final products as well as BHV-1 clearance validation. Specific primers for amplification of BHV-1 DNA was selected, and BHV-1 DNA was quantified by use of SYBR Green I. The sensitivity of the assay was calculated to be $2\;TCID_{50}/ml$. The real-time PCR method was validated to be reproducible and very specific to BHV-1. The established real-time PCR assay was successfully applied to the validation of Chinese hamster ovary (CHO) cell artificially infected with BHV-1. BHV-1 DNA could be quantified in CHO cell as well as culture supernatant. Also the real-time PCR assay could detect $10\;TCID_{50}/ml$ of BHV-1 artificially contaminated in bovine collagen. The overall results indicated that this rapid, specific, sensitive, and robust assay can be reliably used for quantitative detection of BHV-1 contamination during the manufacture of biologics.

치매(痴呆)에 대한 임상 한의학 연구 경향 분석 : 지난 10년간의 국내 논문을 중심으로

이동혁,Lee, Dong Hyuk 대한예방한의학회 2020 대한예방한의학회지 Vol.24 No.1

Objective : In this article, we overviewed clinical Korean Medicine studies on dementia by analyzing papers published in Korea from 2010 to 2019. Methods : We explored research articles on dementia from 5 Korean research databases using the keyword 'dementia' and 'Korean Medicine'. We included original articles and reviews of clinical Korean Medicine and excluded single case study and experiment article of animal model. Then we analyzed them according to the published year, journal type, and research topics. Results & Conclusions : Research topics were classified into 'research trend', 'treatment', 'prevention', 'evaluation', 'medical expenses', 'collaborative practice' and 'others'. The largest number of articles were published in 2011(24.4%). 'Journal of Oriental Neuropsychiatry' was the journal with the most published articles(46.3%). In research topics, the analyses of research trend accounted for the largest portion(39.0%) and the articles about treatment for dementia or evaluation index in Korean medicine had the second largest proportion(14.6%). We hope that various researches for therapeutic effects, mechanisms and preventive effects of Korean Medicine in dementia would be conducted for future study.

고차원 데이터에서 공분산행렬의 추정에 대한 비교연구

이동혁,이재원,Lee, DongHyuk,Lee, Jae Won 한국통계학회 2013 응용통계연구 Vol.26 No.5

공분산 행렬은 다변량 통계분석에서 중요한 역할을 하고 있으며 전통적인 다변량 분석의 경우 표본 공분산 행렬이 참공분산 행렬의 추정량으로 주로 사용되었다. 하지만 변수의 수가 표본의 크기보다 훨씬 큰 고차원 데이터와 같은 경우에는 표본 공분산 행렬은 비정칙행렬이 되어 기존의 다변량 기법을 사용하는 데 적절하지 않을 수가 있다. 최근 이러한 문제점을 해결하기 위해 축소추정, 경계추정, 수정 콜레스키 분해 추정 등의 새로운 공분산 행렬의 추정량들이 제안되었다. 본 논문에서는 추정량들의 성능에 영향을 미칠 수 있는 여러 현실적인 상황들을 가정하여 모의실험을 통해 참공분산 행렬의 추정량들의 성능을 비교하였다. The covariance matrix is important in multivariate statistical analysis and a sample covariance matrix is used as an estimator of the covariance matrix. High dimensional data has a larger dimension than the sample size; therefore, the sample covariance matrix may not be suitable since it is known to perform poorly and event not invertible. A number of covariance matrix estimators have been recently proposed with three different approaches of shrinkage, thresholding, and modified Cholesky decomposition. We compare the performance of these newly proposed estimators in various situations.

경량합성벽체의 전단성능 및 압축내력 평가

이동혁,이상섭,배규웅,문태섭,Lee, Dong Hyuck,Lee, Sang Sup,Bae, Kyu Woong,Moon, Tae Sup 한국강구조학회 2005 韓國鋼構造學會 論文集 Vol.17 No.1

본 논문은 스틸스터드로 구성된 골조에 경량기포모르터를 타설 양생하여 제작한 경량합성벽체의 반복가력 전단실험에 의한 에너지 소산능력과 압축내력 실험을 통한 압축성능의 평가에 대한 연구이다. 경량기포모르터의 유무, 경량기포모르터의 비중(0.6, 0.8, 1.0, 1.2), 마감재(경량기포모르터, OSB, 석고보드) 및 브레이스의 유무, 벽체의 단위(1단위-$900mm{\times}2,400mm$, 2단위-$1,800mm{\times}2,400mm$)를 변수로 하여 실시하였던 경량합성벽체의 전단내력 평가 실험 가운데 반복 실험 결과를 동일 조건의 단조 실험과 비교하였다. 또한 기존 스틸하우스벽체와 경량합성벽체의 압축내력도 실험을 통해 조사하였다. 반복가력 실험결과, 단조가력 실험체의 거동과 약간의 차이를 보이고 있으며 이는 벽체에 채운 경량기포모르터의 비중에 따른 차이라고 생각된다. 압축내력 실험결과, 기존형 실험체에 비하여 경량기포모르터를 타설한 실험체의 최대내력은 2~2.5배, 초기강성은 2~3배 정도 증가하였다. This paper presents the test results and evaluations for the energy dissipation capacity and compressive performance of light-weight hybrid panels. A total of 26 full-scale specimens of light-weight hybrid panels were tested. The parameters include the presence of light-weight foamed mortar, the specific gravity of light-weight foamed mortar (0.6, 0.8, 1.0, 1.2), the finishing materials (light-weight foamed mortar, OSB [Oriented Strand Board], gypsum board), the shape of bracing (x, ~), and the size of panels (1P-900 mm 2,400 mm, 2P-1,800 mm 2,400 mm). The results of the cyclic tests are somewhat different from those of monotonic tests, due to the different specific gravity of light-weight foamed mortar. It was found from the compressive tests that the ultimate strength and initial stiffness are increased by means of light-weight foamed mortar (2~2.5 times in ultimate strength and 2~3 times in initial stiffness).

High-speed atomic force microscopy with phase-detection

이동혁,이현수,N.S. Lee,K.B.Kim,서용호 한국물리학회 2012 Current Applied Physics Vol.12 No.3

In order to improve the scanning speed of tapping mode AFM, we have studied the phase-detection mode AFM with a high frequency (1.5 MHz) cantilever. The phase shifts versus tip-sample distance with different types of samples including polymer, semiconductor, and graphite were measured and the interaction forces were analyzed. It was found that the phase shift in repulsive region is nearly linear as a function of distance, which can be used for feedback control in general, except that some blunt tips cause reversed polarity of phase shift due to excessive energy dissipation. High-speed image with scan rate of 100 Hz was obtained which were controlled with phase shift as a feedback signal.

디지털프린팅법을 이용한 미세 배선 기술의 특허 동향

이동혁,홍태환,Lee, Dong-Hyouk,Hong, Tae-Whan 대한용접접합학회 2008 대한용접·접합학회지 Vol.26 No.2

철심과 권선을 이용한 전류제한기에 적용시킨 안정화층이 다른 YBCO Coated Conductor의 전류제한 특성에 관한 연구

이동혁,두호익,김용진,한병성,임성우,한상철,이정필,Lee, Dong-Heok,Du, Ho-Ik,Kim, Yong-Jin,Han, Byoung-Sung,Yim, Seong-Woo,Han, Sang-Chul,Lee, Jeong-Phil 한국전기전자재료학회 2010 전기전자재료학회논문지 Vol.23 No.10

The yttrium-barium-copper-oxide (YBCO) coated conductor, which supplement the fault of the existing superconducting current-limit materials YBCO thin film, bismuth-strontium-calcium-copper-oxide(BSCCO) wire and bulk, has been improved its mechanical weakness and has high index; hence, after quench YBCO coated conductor could limit the fault current effectively because of fast resistance occurrence speed. Furthermore, it has wide applicable area as an current limit material because it shows different resistance occurrence tendency by the thickness and kind of stabilization material sputtered on the superconducting layer. Therefore, many researchers are carrying out the study of application of YBCO coated conductor to superconducting fault current limiter (SFCL) for making high quality current limit element, based on resistance type. On the other hand, the study for other type except resistance type has been rarely conducted for the application of YBCO coated conductor to SFCL as an current limit element. Consequently, in this study, YBCO coated conductor with different stabilization layer Cu and Stainless steel, is applied to SFCL using iron core and coil, and examine the many index points as an current limit element, such as current limit characteristic, the tendency of resistance occurrence, response time, the temperature trend for stability.

세포배양 유래 생물의약품 생산 공정에서 Reovirus Type 3 안전성 검증을 위한 Real-Time RT-PCR

이동혁,정효선,김태은,오선환,이정숙,김인섭,Lee, Dong-Hyuck,Jeong, Hyo-Sun,Kim, Tae-Eun,Oh, Seon-Hwan,Lee, Jung-Suk,Kim, In-Seop 한국미생물학회 2008 미생물학회지 Vol.44 No.3

세포배양 유래 생물의 약품 생산 공정에서 다양한 바이러스가 오염된 사례가 있기 때문에 바이러스 안전성 검증이 필수적이다. Reovirus type 3 (Reo-3)는 동물 세포주와 동물 세포 배양 공정에 오염되는 대표적인 바이러스이다. 세포배양 유래 생물의약품의 Reo-3 안전성을 확보하기 위해, 세포주, 원료물질, 제조공정, 완제품에서 Reo-3를 정략적으로 검출하고, 제조공정에서 Reo-3 제거 검증을 위한 시험법으로 활용이 가능한 real-time RT-PCR 시험법을 확립하였다. Reo-3에 특이적인 primer를 선별하였으며, 형광염료 SYBR Green I을 사용하여 Reo-3 RNA 정략 검출 시험법을 최적화하였다. 세포배양법에 의한 감염역가와 비교한 결과 real-time RT-PCR 민감도는 $3.2{\times}10^0\;TCID_{50}/ml$이었다. 확립된 시험법의 신뢰성(reliability)을 보증하기 위해 시험법 검증을 실시한 결과 특이성(specificity)과 재현성(reproducibility)이 우수함을 확인하였다. 확립된 real-time RT-PCR을 생물의약품 제조공정 검증에 적용할 수 있는지 확인하기 위하여 인위적으로 Reo-3를 오염시킨 CHO 세포에서 Reo-3 검출 시험을 실시한 결과 Reo-3를 감염시킨 CHO 세포와 세포배양 상청액에서 Reo-3를 정략적으로 검출할 수 있었다. 또한 바이러스필터 공정에서 Reo-3제거 효과를 감염역가 시험법과 비교 검증한 결과 더 빠른 시간에 동일한 결과를 얻을 수 있었다. 위와 같은 결과에서 확립된 Reo-3 real-time RT-PCR 시험법은 생물의약품 안전성 보증을 위한 세포주 검증, 생물의 약품 생산 공정 검증, 바이러스 제거 공정 검증 등에서 감염 역가 시험법을 대신할 수 있는 신속하고, 특이성과 민감성이 우수한 시험법임을 확인하였다. Validation of viral safety is essential in ensuring the safety of mammalian cell culture-derived biopharmaceuticals, because numerous adventitious viruses have been contaminated during the manufacture of the products. Mammalian cells are highly susceptible to Reovirus type 3 (Reo-3), and there are several reports of Reo-3 contamination during the manufacture of biopharmaceuticals. In order to establish the validation system for the Reo-3 safety, a real-time RT-PCR method was developed for quantitative detection of Reo-3 in cell lines, raw materials, manufacturing processes, and final products as well as Reo-3 clearance validation. Specific primers for amplification of Reo-3 RNA was selected, and Reo-3 RNA was quantified by use of SYBR Green I. The sensitivity of the assay was calculated to be $3.2{\times}10^0\;TCID_{50}/ml$. The real-time RT-PCR method was proven to be reproducible and very specific to Reo-3. The established real-time RT-PCR assay was successfully applied to the validation of Chinese hamster ovary (CHO) cell artificially infected with Reo-3. Reo-3 RNA could be quantified in CHO cell as well as culture supernatant. When the real-time RT-PCR assay was applied to the validation of virus removal during a virus filtration process, the result was similar to that of virus infectivity assay. Therefore, it was concluded that this rapid, specific, sensitive, and robust assay could replace infectivity assay for detection and clearance validation of Reo-3.