세파졸린의 산-염기의 거동

이동선,김수현,이윤중 서울여자대학교 자연과학연구소 1990 자연과학연구논문집 Vol.1 No.-

항생물질 cefazolin(CZH)의 산-염기 해리반응에 관해 실험하여 다음과 같은 결론을 얻었다. 1. 전위차법으로 25℃에서 측정한 CZH의 pKa 값은 3.78(±0.05) 이었다. 전도도법으로 측정한 경우 pKa=3.75였으며, [CZNa]/[CZH] = 1.00의 완충용액으로 측정하였을 때에는 pKa = 3.41이었다. 2. CZNa+CZH 혼합용액의 전기 전도도는 [CZNa] 농도가 증가될수록 공통이온외 영향으로 약간씩 감소되었다. 3. CZH의 용해도는 92.8mg/100mL이고, 해리도는 0.4650으로 산출되었다. 4. CZH의 pKa 값은 5~50℃ 범위에서 커다란 변화가 없었다. 5. CZH 산해리반응을 열역학적으로 고찰할 때, ΔG°=+19.995kJ/mol이었다. 따라서 CZH 외 난용성을 증명할 수 있었다. 6. 산-염기 분율조성을 고찰한 결과 pH<3.78의 수용액중에서는 산성형 CZH가 주된 화학종이고 pH>3.78인 용액중에서는 염기형인 CZ^(-)가 주된 화학종임을 알았다. 7. 5% 포도당 주사액중에서는 CZH가, Hartman용액중에서는 CZ^(-)가 주된 화학종이며, 생리식염 주사액중에서는 두 화학종이 비숫하게 분포하였다. 8. 생체액중에서의 CZH거동을 고찰한 결과 동맥혈, 정맥혈, 안방수, 누액, 십이지장, 회장, 소장 등에서는 99%이상 CZ^(-)형태로 분포하고, 위장에서는 약 99%가 CZH형태로 분포하리라 추정되었다. 9. UV 및 IR 흡광특성은 CZNa가 CZH에 비하여 장파장 이동을 나타내었다. The purpose of this study was to determine the acid dissociation constant of cefazolin antibiotics potentiometrically and conductometricaliy, and to identify the principal species in parenteral infusions and body fluids with different pH values. The pKa of cefazolin was 3.78(±0.05) at 25℃, and the pKa value of cefazolin was almost constant at temperature range of 5~50℃. As the concentration of basic form in the mixture of cefazolin(CZH) and cefazolin sodium(CZNa) increased the conductance decreased still further because of the common ion effect. The computed solubility of CZH was 92.8mg/100mL, the fractrion of dissociation a was found 0.4650. The low solubility was demonstrated thermodynamically. The Gibb's energy change for the dissociation was +19.995kJ/mol, reaction is disfavored. The acidic species(CZH) was the predominant form at pH<3.78, the basic species(CZ^(-)) was the principal form at pH>3.78. The results of pH measurement it was assumed that CZH was major in 5% dextrose inj., CZ^(-) in Hartman's solution, and CZH and CZ^(-) existed almost equally in saline solution. It was also assumed that the major form was basic in aqueous humor, blood venous, duodenum, ileum distal, lacrimal fluids, and intestine microsurface, while acidic in the stomch. UV and IR spectra of the basic form, CZ was showed bathochromic shift.

방선균주 7489가 생산하는 DNA Topoisomerase 1 저해제에 관한 연구

이동선,하상철,이상용,김종국,홍순덕 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.1

미생물로 부터 topoisomerase Ⅰ저해물질을 탐색하던 중 Streptomyces melanosporofaciens 7489 균주가 topoisomerase 활성을 저해하는 것을 발견하였다. 이에 균주가 생산하는 활성물질을 분리정제한 후 구조를 결정하였고 정제된 물질에 대한 활성을 조사하였다. 균체로 부터 acetone 추출, ethyl acetate 추출, silica gel column chromatography, preparative TLC 및 HPLC등을 통하여 topoisomerase 저해물질 7489-1을 정제한 후 UV, ^1H-NMR, ^13C-NMR, DEPT and EI-MS등의 기기분석을 한 결과 저해물질은 dibutyl phthalate로 결정되었다. 789-1의 topoisomerase Ⅰ 저해활성은 1μM에서 10μM까지 점진적으로 감소하였으며 25μM에서 완전히 저해되었다. During the screening of inhibitor of DNA topoisomerase I from microbial secondary metabolites, Streptomyces melanosporofaciens 7489 which was capable of producing high level of inhibitor was selected from soil. The active compound (7489-1) which was capable of producing high level of inhibitor was selected from soil. The active compound (7489-1) was purified from the culture broth by solvent extraction, silica gel column chromatography and HPLC. The inhibitor was identified as dibutyl phthalate by spectroscopic methods of UV, ^1H-NMR, ^13C-NMR, DEPT and EI-MS. 7489-1 showed a strong inhibitory activity against topoisomerase I with 10 μM of IC_50 value.

쇼트피닝가공한 평기어의 최적 피닝강도 탐색에 관한 연구

이동선,이국진,김태형,정성균 한국공작기계학회 2005 한국공작기계학회 춘계학술대회논문집 Vol.2005 No.-

The shot peening process is often used to improve fatigue properties of metal parts. Among them, It is the most use in an auto-component. In order to achieve optimum, repeatable and reliable fatigue enhancement from the shot peening process, the important shot peening parameters must be controlled. In this paper, the optimum peening intensity (Almen intensity) condition is investigated by experiment..The Spur Gear steel was used to investigate shot peening effects. The fatigue life at σ_(a)=1,050 and σ_(a)=1,250MPa first gently increases, then drops gently as peening intensity increases compared with unpeened specimen. Experimental results show that the optimum peening intensity range is 0.391~0.434mmA..So the fatigue strength and fatigue life have been tremendously increased by optimum-peening treatment. However, the fatigue strength and fatigue life have been decreased by over peeing.

Lumogallion에 依한 Prifinium Bromide의 螢光定量

安文圭,李東宣 慶星大學校 1986 부산수산대학 논문집 Vol.7 No.2

Lumogallion is used as coloring and fluorescent reagent for Ga, In, Mo, Nb, Sc, Sn. Prifinium bromide could be extracted as ion association with lumogallion from aqueous acidic solution, and then determined fluorometrically after additon of aluminium(Ⅲ) ion. The analysis was carried out as follows; To a 1ml portion of prifinium bromide(0.1∼10㎍/ml), 1ml of 0.01 W/V% lumogallion solution, and 10ml of choroform are added. The mixture is stirred for 1 minute. After standing for a few minutes, the upper aqueous phase is removed, and organic phase is transferred to 1ml of 0.1% Al(NO₃)₃ethanol solution. After mixing, and then standing for 30minutes at room temperature, the fluorescence intensity is measured with excitation maximum at 469nm and with emission maximum at 573nm. The reagent blank is run through the whole procedure. The calibration curve showed a good linearity over the range of 0.1∼10㎍/ml sample. The coefficients of variation for the present method were 2.3 to 2.6%. This method is useful for the determination of micro amounts of prifinium bromide contained in pharmaceutical preparations and applied to the pyrrolidinium salts.

이온 이동도 분석법의 원리와 응용

이동선 서울여자대학교 자연과학연구소 1997 자연과학연구논문집 Vol.9 No.-

Ion mobility spectrometry is discussed in this review paper. Included are sections dealing with instrumentation, basic theory of ion mobility in gas phase. Analytical applications of ion mobility spectrometer as a stand-alone spectrometer or as a detector following gas, liquid, and supercritical fluid chromatography are described.

우태아혈청 및 성인뇨중 β-에스트라디올, 에스트론, 에스트리올의 역상 HPLC

신희수,이동선 서울여자대학교 자연과학연구소 1997 자연과학연구논문집 Vol.8 No.-

A method for reversed phase high performance liquid chromatographic separation of estriol, estradiol, and estrone in biological samples such as human urine, fetal bovine serum is described. Estrogens were determined by HPLC following solid phase extraction using Sepak C18 cartridge for sample clean up. Chromatographic separation is completed in 10 min using mobile phase of 60v/v% acetonitrile in water and Spheri-5 RP-18 column (4.6mm*22cm, 5micron) at flowarte of 0.5ml/min. UV-detection of eluted compounds was accomplshed at 280nm. Retention time of triol was shorter than those of diol and monool. These result suggests than triol is polar than diol and monool.

천연 아로마 자원, 추출 및 분석기술

이동선 江原大學校 附設 環境硏究所 2005 環境硏究 Vol.22 No.-

癩患者의 下肢 切斷術 後 合倂症에 關한 臨床的 觀察

李東善 全南大醫大 1981 전남의대학술지 Vol.18 No.1

식품 살균공정의 에너지 분석

변유량,이동선 慶南大學校 附設 工業技術硏究所 1986 硏究論文集 Vol.4 No.-

저산성 식품의 회분식 고온 스팀살균시 에너지소비를 요인별로 분석하였다. 물질수지 및 에너지수지 방정식으로부터 각 항목별 열손실 및 열사용을 예측하고 전체 에너지소비를 구하였다. 전도열전달 모델식품으로서 5% 벤토나이트용액과 대류열전달 모델식품으로서 물을 사용하여 301-7호관에 포장하여 열침투특성을 구하고 110℃, 121℃, 130℃에서 살균하는 조건에 대하여 분석하였다. 에너지 소비는 110℃에서가 121℃와 130℃에 비해서 현저히 많았고 요인별로 볼 때 이는 bleeding과 대류 및 복사열손실에 의한 것으로 나타났다. 또한 조건별 가열효율에 대하여 검토하였다. A procedure was developed for predicting energy consumption of batch type thermal processing of food. From mass and energy balance equations various energy usages or losses were estimated for steam sterilization of model food system in No. 301-7 can(Ø74.1×113.0㎜) at three different temperatures. Selected models were 5% bentonite solution for conductive food and tap water for convective food. Total steam or energy consumption was higher at 110℃ than at two other higher temperatures(121℃ and 130℃). High energy consumption at low sterilization temperature was mainly due to high bleeding steam energy and convective and radiative heat losses. Thermal energy efficiency was also disscussed.

抗히스타민劑의 吸光度比法에 의한 定量

李允中,李康春,李東宣,李振九 成均館大學校 1980 論文集 Vol.28 No.-

The absorbance ratio method was applied to the quantitative determination of antihistamines, such as chlorpheniramine maleate(CPM), carbinoxamine maleate(CBM) and diphenhydramine hydrochloride. The influences of indicator and solvent concentration on the precision and accutacy of determination were investigated. And it was found that the effects of temperature and wavelength was negligible in case of averaging conditions. The standard deviations for three antihistamines tested were within 0.13%. As the results, the determination of antihistamines could be carried out easily, rapidly and accurately by the absorbance ratio method.