http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
B형 간염 간세포암 환자에서의 간절제 후 혈청 HBV DNA 역가의 변화
이해원(Hae Won Lee),서경석(Kyung-Suk Suh),김주현(Joohyun Kim),신우영(Woo Young Shin),이남준(Nam-Joon Yi),이건욱(Kuhn Uk Lee) 한국간담췌외과학회 2010 한국간담췌외과학회지 Vol.14 No.1
Purpose: Reactivation of hepatitis B virus (HBV) replication after hepatic resection might be a significant risk factor for prognosis in patients with chronic hepatitis B. The purpose of the present study was to investigate the changing pattern of serum HBV DNA titer after hepatic resection and to assess the incidence of reactivation of HBV replication. Methods: Among HBV-positive patients who underwent hepatic resection for hepatocellular carcinoma, thirty-six patients with preoperative serum HBV DNA titer ≥3 log10copies/mL were enrolled. Serum DNA titers were examined before the operation, on the second and seventh postoperative days, and one month after the operation. Results: The serum DNA titer decreased on the second postoperative day (p=0.078). The DNA level, however, had substantially returned to preoperative values by the seventh postoperative day (p<0.001). For most patients, the postoperative DNA titer reached its zenith on the seventh postoperative day or one month after the operation. The zenith level was higher (by 0.49±0.25 log10copies/mL) than preoperative levels although this difference just missed significance (p=0.068). Although postoperative reactivation of HBV replication emerged in 6 patients, only one of those patients developed postoperative hepatitis. Overall, four patients developed postoperative hepatitis and all of them had high postoperative HBV DNA levels (over 6 log10copies/mL). Conclusion: Although serum HBV DNA titers tended to increase postoperatively, routine antiviral therapy might be unnecessary because of the low incidence of postoperative hepatitis. High postoperative DNA levels, however, might be a risk factor for hepatitis, and postoperative follow-up of serum HBV DNA levels might be necessary in HBV-positive patients with hepatic resection.
Outcomes for patients with HCV after liver transplantation in Korea: a multicenter study
김종만,이광웅,송기원,Bo-Hyun Jung,Hae Won Lee,이남준,Choon Hyuck David Kwon,Shin Hwang,서경석,Jae-Won Joh,Suk-Koo Lee,이승규 대한외과학회 2016 Annals of Surgical Treatment and Research(ASRT) Vol.90 No.1
Purpose: HCV-related liver disease is the most common indication for liver transplantation (LT) in Western countries, whereas HCV LT is rare in Korea. We conducted a survey of HCV RNA-positive patients who underwent LT and investigated the prognostic factors for patient survival and the effects of immunosuppression. Methods: We retrospectively reviewed the multicenter records of 192 HCV RNA-positive patients who underwent LT. Results: The 1-, 3-, and 5-year overall survival rates were 78.8%, 75.3%, and 73.1%, respectively. Excluding the cases of hospital mortality (n = 23), 169 patients were evaluated for patient survival. Most patients were genotype 1 (n = 111, 65.7%) or genotype 2 (n = 42, 24.9%). The proportion of living donors for LT (n = 135, 79.9%) was higher than that of deceased donors (deceased donor liver transplantation [DDLT], n = 34, 20.1%). The median donor and recipient ages were 32 years and 56 years, respectively. Twenty-eight patients (16.6%) died during the observation period. Seventy-five patients underwent universal prophylaxis and 15 received preemptive therapy. HCV recurrence was detected in 97 patients. Recipients who were older than 60, received DDLT, used cyclosporine, or suffered acute rejection had lower rates of survival. Conclusion: Patent survival rates of HCV patients after LT in Korea were comparable with other countries
최영록 ( Youngrok Choi ),이광웅 ( Kwang-woong Lee ),이해원 ( Nam-joon Yi ),이남준 ( Hae Won Lee ),서경석 ( Kyung-suk Suh ) 대한간암학회 2013 대한간암학회지 Vol.13 No.1
Malignant portal vein thrombosis is a contraindication to liver transplantation for hepatocellular carcinoma because of the high risk of its recurrence and the poor patient survival. With a newly developed immunosuppressant and a chemotherapeutic agent, however, living donor liver transplantation can be considered for a patient of hepatocellular carcinoma, showing a slow growth rate and good response for transarterial chemoembolization. We report a HBV related liver cirrhosis patient with HCC and portal vein tumor thrombus who underwent living donor liver transplantation and survived without recurrence of hepatocellular carcinoma for 18 months in our center.
Cytodex 3 미립담체를 이용한 간세포의 구상체 배양에서 간세포와 미립담체 간의 최적 비율
신우영(Woo Young Shin),이건욱(Kuhn Uk Lee),이해원(Hae Won Lee),조응호(Eung-Ho Cho),이남준(Nam-Joon Yi),서경석(Kyung-Suk Suh) 대한외과학회 2007 Annals of Surgical Treatment and Research(ASRT) Vol.73 No.3
Purpose: The mass cultivation of functional hepatocytes is a key factor of a bioartificial liver. Combining spheroid and microcarrier cultures has been applied for enhancing the cell viability and metabolic activities. Hence, the optimal number of hepatocytes per microcarrier was investigated. Methods: Firstly, spheroid cultures were carried out with 1 g Cytodex 3 microcarrier plus 2×10?, 4×10? and ?×10? viable hepatocytes per flask. The numbers of hepatocytes per microcarrier were approximately 666.7, 133.3 and 26.7, respectively. The control group consisted of a spheroid culture of 4×10? hepatocytes without any microcarrier. According to the primary experimental results, spheroid cultures with 1×10? of hepatocytes plus 1 g, 2 g and 3 g of the Cytodex 3 microcarrier were performed. The numbers of hepatocytes per microcarrier were approximately 33.3, 16.7 and 11.1, respectively. The control group consisted of a spheroid culture of 1×10? hepatocytes. The cell viabilities were assayed using a Cell Counting Kit-8; with the albumin production assayed using ELISA. Results: According to the primary experiment, the group consisting of 26.7 hepatocytes per microcarrier showed the highest viability (P<0.01). However, there was no statistical difference in the albumin production between the groups (P=0.744). The second Experiment showed the groups consisting of 11.1 and 16.7 hepatocytes per microcarrier had higher viabilities than the other hepatocyte and control groups (P<0.01). The albumin production was similar for each group (P=0.187). Conclusion: With respect to their application to a bioartificial liver, about 130 hepatocytes per microcarrier was appeared to be good for the mass cultivation of a hepatocytes spheroid culture using the Cytodex 3 microcarrier.
70% 간절제술을 받은 쥐에서 피브린젤을 이용한 중간엽줄기세포의 생체내 간세포 분화
신우영(Woo Young Shin),정성은(Sung Eun Jung),민혜숙(Hye Sook Min),이해원(Hae Won Lee),조응호(Eung-Ho Cho),이남준(Nam-Joon Yi),서경석(Kyung-Suk Suh),이건욱(Kuhn Uk Lee) 한국간담췌외과학회 2010 한국간담췌외과학회지 Vol.14 No.2
Purpose: To investigate the differentiation of rat bone marrow-derived mesenchymal stem cells (MSCs) into hepatocytes by cell transplantation using fibrin gels in a 70% hepatectomized rat model. Methods: MSCs were isolated from Sprague-Dawley rats. MSCs (1.5×107 cells) were mixed with fibrin gels and injected immediately into the abdominal cavity of 70% hepatectomized rats. Fibrin-gels consisted of 500 IU/ml of thrombin and 90 mg/ml of fibrinogen. Transplanted MSCs in the fibrin scaffold were retrieved from surgically opened peritoneal cavities of rats on days 5, 10, 15, and 21 after the operation. The specimens were analyzed histologically and immunohistochemically. Results: On H&E staining, MSCs from hepatectomized rats had changed to a round shape, while MSCs of the control group kept their spindle shape. When the fibrin matrix was biodegraded at day 15, the morphology of the MSCs had changed to hepatocyte-like cells without sinusoids and the hepatocyte-like cells had formed a three-dimensional tissue permitting cell-to-cell contacts within the matrix. On immunohistochemistry, MSCs expressed the hepatocyte markers cytokeratin 18, albumin, and alpha-fetoprotein, after 15 days of transplantation. Conclusion: When bone marrow-derived MSCs are transplanted using fibrin gels in the 70% hepatectomized rat, MSCs differentiate into hepatocyte-like cells and are conglomerated so that they form three-dimensional tissue-like hepatocytes without sinusoids.