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양식동남아산 뱀장어(Anguilla bicolor pacifica)의 Heterosporis anguillarum 감염
김진도 ( Jin-do Kim ),도정완 ( Jeong-wan Do ),최혜승 ( Hye-sung Choi ),조혜인 ( Hyae-in Jo ),이남실 ( Nam-sil Lee ),김영대 ( Young-dae Kim ) 한국환경생물학회 2014 환경생물 : 환경생물학회지 Vol.32 No.4
Shortfin eel (Anguilla bicolor pacifica) is a species of commercial importance and its production is greatly affected due to the infection by Heterosporis anguillarum. In this study, we evaluated the effect of H. anguillarum infection on the growth of Shortfin eel. A disease that trunk muscle of cultured shortfin eel, Anguilla bicolor pacifica, were irregular and resulted in death, breakout of the commercial eel culture farm. We observed that the trunk muscle of infected eels were irregular and represented white or yellowish externally. Histopathologically, a great numbers of large or small spores and sporophorocysts were also observed in degenerated muscle layer. The cloning of specific gene of H. anguillarum, encoding small subunit ribosomal RNA (SSU-rRNA)was amplified by the polymerase chain reaction(PCR) from the muscle lesion of diseased eel. The size of clone gene is well matched with the size of small subunit ribosomal RNA of H. anguillarum and thus confirming the infection by H. anguillarum.
Aeromonas veronii의 실험적 감염에 의한 양식 메기, Silurus asotus의 병리학적 변화
김진도 ( Jin Do Kim ),도정완 ( Jeong Wan Do ),최혜승 ( Hye Sung Choi ),서정수 ( Jung Soo Seo ),정승희 ( Sung Hee Jung ),조혜인 ( Hyae In Jo ),박명애 ( Myung Ae Park ),이남실 ( Nam Sil Lee ),박성우 ( Sung Woo Park ) 한국환경생물학회 2013 환경생물 : 환경생물학회지 Vol.31 No.4
Recently, a new disease showing symptoms such as epidermal exfoliation and muscular necrosis occurred in cultured Korean catfish. Although the mortality of fishes was low but the economic damages owing to loss of commercial value were severe. The authors isolated the causative agent from diseased fish and observed pathological changes both in naturally and artificially infected fish. The causative bacteria was identified as Aeromonas veronii. Subsequently we observed the daily death and pathological symptoms of artificially infected fish with Aeromonas veronii. Symptoms of artificially infected fish were similar to those of naturally infected fish and all fish died within 7 days after infection. Histopathological changes on the naturally infected fish revealed severe congestion and necrotic degeneration in the liver, spleen and kidney. Some bacterial aggregates with inflammatory degeneration were observed in the heart, and congestion and fibrosis in the lamina propria of digestive tube were predominant. In artificially infected fish, skin erosion and necrotic degeneration of muscle tissue around injected region were particularly manifested. Degeneration of hepatocytes in liver and hyalic degeneration around ellipsoids in spleen were partially observed. However, there were no predominant signs in digestive tube in artificially infected fish.
In-situ hybridization 법을 사용한 양식 넙치, Paralichthys olivaceus의 바이러스 감염 질병 특성 고찰
도정완 ( Jeong Wan Do ),이남실 ( Nam Sil Lee ),정승희 ( Sung Hee Jung ),김경길 ( Kyung Kil Kim ),최혜승 ( Hye Sung Choi ),박정우 ( Jeong Woo Park ),김이청 ( Yi Cheong Kim ) 한국어병학회 2013 한국어병학회지 Vol.26 No.3
PCR (polymerase chain reaction) 법은 신속하고 정확하여 바이러스성 질병진단을 위해 널리 사용되지만 조직병리학적인 정보를 제공하지 못한다. 반면에 in-situ hybridization (ISH) 법을 사용하면 바이러스를 빠르게 검출할 수 있을 뿐 아니라 조직에서의 분포도알 수 있다. 본 연구에서는 RSIV, VHSV, 그리고 VNN바이러스들의 조직내 분포 및 조직 병리학적인 특성을 확인하기 위하여 이 바이러스들에 감염된 에 감염된 양식 넙치의 어류의 다양한 조직들을 대상으로 ISH 법을 적용하였다. 그 결과 이들 세 종류의 바이러스가 각각 다른 조직 및 세포들에 감염함을 확인 할 수 있었다. 본 연구 결과는 ISH법이 어류 병원성 바이러스의 신속 검출 뿐 아니라 조직 병리학적인 특성 확인에도 유용함을 제시한다. Polymerase chain reaction (PCR) is the most rapid and widely used method to detect viral pathogens. However, this method does not provide histopathologic nature of the virus. In situ hybridization (ISH) with oligonucleotide probes is attractive because it is a rapid method for detection and identification of viral pathogens at sites of tissue infection. In order to understand the histopathologic characterictics of Red sea bream iridovirus (RSIV), viral-hemorrhagic septicemia (VHS) virus and viral nervous necrosis (VNN) virus to cultured olive flounder, we her applied ISH method to various kinds of olive flounder tissues with PCR-positive for these three viruses. We found that these viruses showed different tissue tropism and were detected from different cell types. Our results suggest that ISH is useful not only in rapid detection of viral pathogens but also in understanding the histopathologic characters of specific viral pathogens.
김종구(Jong-Gu Kim),장효상(Hyo-Sang Jang),강훈(Hoon Kang),이남도(Nam-do Lee),권민선(Min-sun Kwoun) 한국해양환경·에너지학회 2011 한국해양환경·에너지학회 학술대회논문집 Vol.2011 No.11
육상기원 오염물질의 관리를 위해서는 육역에서 발생되는 오염부하 부하량을 정확하게 산정하여야 하며, 이러한 자료를 바탕으로 해역에서의 오염물질의 영향을 평가하고 관리하기 위한 모델작업이 필요하게 된다. 해역의 오염물질 영향을 평가하는 모델에는 수치생태모델과 박스모델 등이 개발되어 있다. 육상기인 오염원 관리 유형중 비점오원형인 함평연안을 대상으로 물질순환의 특성과 영양염의 양적인 거동을 밝히기 위하여, 선진외국에서 연안해역의 해양환경 관리를 위하여 육상과 해양의 물질순환 알고리즘을 정량화한 LOICZ(Land Ocean Interaction Coastal Zone)을 이용하였다. 물질수지 산정을 위한 대상물질은 보존성물질(염분)과 비보존성물질(DIP, DIN)로서 BOX내에서 오염물질의 유입과 유출 특성을 분석하였다. 물질수지 결과를 보면, 함평만의 담수 체류시간은 Box1에서 동계 19.97일, 춘계 2.71일로 나타났고, Box2에서 0.22일 이하였다. DIP 경우 동계에는 DIP 방출이 일어났고, 춘계에는 DIP흡수가 일어났으나, DIN의 경우 동계탈질이 일어났고, 춘계에는 질소 고정이 일어났다. The estimation of material cycle of pollutants is necessary for the environmental management in coastal zone. Model for material budgets are useful tools to understand the phenomena of natural system and to provide an insight into the complex process including physical, chemical and biological processes occuring in natural system. The goal of this study is survey to characteristics of pollutant loads and estimation of material budgets in Hampyeong Bay which is nonpoint source type. The residence time of fresh water was calculated to be 2.71∼19.97day in Box 1 and 0.22day in Box 2. The outflow mass of DIP and DIN was larger than the inflow mass during winter. But, to spring season it appeared to opposition.