1.5 T 자기공명영상기기에서 수소 자기공명분광법을 이용한 모델용액 내 포도당의 정량분석

이경희,이정희,조순구,김용성,김형진,서창해,Lee, Kyung-Hee,Lee, Jung-Hee,Cho, Soon-Gu,Kim, Yong-Seong,Kim, Hyung-Jin,Suh, Chang-Hae 대한영상의학회 2002 대한영상의학회지 Vol.46 No.1

목적: 1.5T 생체용 자기공명영상기기를 이용한 수소자기공명분광법으로 용액 내 물질의 정량분석에 대한 가능성을 알아보고자 하였다. 대상과 방법: 0.01%에서 50%까지의 여러 농도를 갖는 포도당+증류수 혼합액과 0.01%에서 20%까지의 여러 농도를 갖는 포도당+증류수+에탄올 혼합액의 모델용액을 만들어 생체용 자기공명영상기기와 시험관 nuclear magnetic resonance(NMR)분광기에서 각각 수소자기공명분광법을 시행하여 스펙트럼을 얻었다.스펙트럼 상에서 포도당 농도에 따른 포도당/물, (포도당+에탄올)/물, (포도당+에탄올)/에탄올 피크의 면적 비의 변화를 구하였고, 통계처리는 상관분석과 단순선형회귀분석을 시행하였고 회귀식을 산출 하였다.또한 생체용 자기공명영상기기를 이용하여 얻은 결과가 객관적인지 알아보기 위해 시험관 NMR 분광기에서 얻은 결과와의 상관관계를 분석하였다. 결과: 생체용 자기공명영상기기를 이용한 스펙트럼상 포도당/물, (포도당+에탄올)/물, (포도당+에탄올)/에탄올 피크의 면적 비는 포도당 농도변화에 대하에 일정하게 증가하는 통계적으로 의미 있는 변화를 보였고 그 결과에 따라 회귀식을 구할 수 있었다. 생체용 자기공명영상기기와 시험관 NMR 분광기를 이용하여 얻은 모든 결과에 대한 상관분석에서 통계적으로 의미 있는 상관관계를 보여 생체용 자기공명영상기기에서 얻은 수소자기공명분광 소견을 객관화 할 수 있었다. 따라서 생체용 자기공명영상기기를 이용하여 얻은 스펙트럼에서 용액 내 물질의 피크면적비를 구함으로써 농도를 정량할 수 있는 결과를 얻을 수 있었다. 결론: 생체용 자기공명영상기기를 이용하여 얻은 수소자기공명분광 스펙트럼에서도 시험관 NMR분광기로 얻은 스펙트럼과 마찬가지로 단순 포도당 용액, 포도당에 에탄올을 혼합한 용액에서 포도당/물, (포도당+에탄올)/물, (포도당+에탄올)/에탄올 피크의 면적 비를 측정함으로써 당의 정량분석이 가능하였다. 본 연구의 결과는 생체수소자기공명분광법이 환자의 체액에 대한 정량적 분석에 이용될 수 있다는 가능성을 시사한다고 생각한다. Purpose: To evaluate the feasibility of proton magnetic resonance spectroscopy ($^1H-MRS$) using a 1.5T magnetic resonance (MR) imager for quantification of the contents of model solutions. Materials and Methods: We prepared model solutions of dextrose+water and dextrose+water+ethanol at dextrose concentrations of 0.01% to 50% and 0.01% to 20%, respectively. Using these solutions and a 1.5T MR imager together with a high-resolution nuclear magnetic resonance (NMR) spectroscope, we calculated the ratios of dextrose to water peak, (dextrose+ethanol) to water peak, and (dextrose+ethanol) to ethanol peak, as seen on MR and NMR spectra, analysing the relationships between dextrose concentration and the ratios of peaks, and between the ratios of the peaks seen on MR spectra and those seen on NMR spectra. Results: Changes in the ratios between dextrose concentration and dextrose to water peak, (dextrose+ethanol) to water peak and (dextrose+ethanol) to ethanol peak, as seen on MR spectra, were statistically significant, and there was good linear regression. There was also close correlation between the ratios of the observed on MR and NMR spectra. The results depict the quantification of dextrose concentration according to the ratios of spectral peaks obtained by proton MRS at 1.5T. Conclusion: Using proton MRS at 1.5T, and on the basis of the ratios of spectcal peaks, it was possible to quantify the concentration of dextrose in model solutions of dextrose+water and dextrose+water+ethanol. The results of this study suggest that for quantifying the contents of biofluids, the use of low-tesla $^1H-MRS$ is feasible.

LPS로 유발된 대식세포의 염증반응에 대한 청상보하탕(淸上補下湯)의 효과

이경희,김홍렬,정희재,이형구,Lee, Kyung-Hee,Kim, Hong-yeoul,Jung, Hee-Jae,Lee, Hyung-Koo 대한한방내과학회 2008 大韓韓方內科學會誌 Vol.29 No.1

Background and Objective : Chungsangboha-tang (CSBHT) has analgesic, sedative, anti-convulsive and anti-histamine effects, so it alleviates the symptoms of asthma. For the comparison of anti-inflammatory effect(s) on CSBHT, PD098059 was used as a negative control. Materials and Methods : This study emphasized THP-1 cells, which had been well characterized as a human monocytic leukemic cell line. The cells resemble monocytes with respect to several criteria and can be differentiated into macrophage-like cells by treatment with PMA. By using the MTS assay, it was possible to prove the safety of CSBHT. Results : Results shows that the CSBHT did not affected cell survival within $10^{1}$ ng/ml to $10^{5}$ ng/ml. Especially, $10^{5}$ ng/ml CSBHT treated cells show 70% deduction of $TNF-{\alpha}$ gene expression against that of LPS treated group. Furthermore, $IL-1{\beta}$, IL-6, IL-8, IL-10 and $TNF-{\alpha}$ levels are down-regulated when treated with CSBHT with concentrations up to 100 ug/ml on monocyte-derived macrophages. Interestingly, CSBHT-treated samples showed that overall transcriptional activities were down-regulated to 20% of that of PD098059 ($TNF-{\alpha}$ inhibitor). At protein level, the expression of $TNF-{\alpha}$ showed similar results as that of transcriptional activity. Results show that the protein level decreased more in the CSBHT-treated group (487 ${\pm}$ 87 pg/ml) than in the LPS-treated group (703 ${\pm}$ 103 pg/ml). In addition, the protein level of IL-8 in the CSBHT treated-group (9.84 ${\pm}$ 3.28 ng/ml) decreased similar as the expression of the control and PD098059-treated groups. Conclusion : CSBHT affects immune response, especially allergic responses and suppression of inflammatory reaction. The results provide us an alternative way to care for clinical inflammatory diseases, not only asthma but also the other possible general inflammatory and allergic diseases.

논문편 : 식품표시에 대한 소비자 행동 연구

이경희,여정성 ( Kyung Hee Lee,Jung Sung Yeo ) 대한가정학회 1996 Human Ecology Research(HER) Vol.34 No.5

실측을 통한 신축공동주택의 실내공기질에 관한 연구

이경희,배종수,조성우,박민용,박창섭,최정민,Lee Kyung-Hee,Bae Jong-Soo,Cho Sung-Woo,Park Min-Yong,Park Chang-Sub,Choi Jeong-Min 한국주거학회 2006 한국주거학회 논문집 Vol.17 No.4

The harmfulness of HCHO and VOCs from construction material and furniture has been increased gradually. It must be need to prevent pollution materials' accumulation indoor effectively and to remove very small amount of harmful pollution materials in various plans, because these kinds of pollution materials greatly affect human body, Therefore, this study is focused to find out the improvements of Indoor Air Quality in execution of natural ventilation and bakeout to reduce indoor chemical pollution materials or not. After effects of indoor air quality by natural ventilation and bake-out being examined, it is follows the conclusion. As for the density change of TVOC and Toluene according to time lapse, in case the middle and high-story areas have bake-out, the density increased once, but it showed the gradual decrease after bake-out was stopped, and it was shown that it exceeded the standards recommended for newly built apartment. The bake-out is effective to discharge the HCHO and TVOC from the construction material and the furniture, and the natural ventilation is effective remove the indoor pollution materials.

윈도우 커버링용 PET 난연 직물의 마모 및 역학특성

이경희,전연희,안승국,Lee, Kyung Hee,Jeon, Youn Hee,An, Seung Kook 한국섬유공학회 2013 한국섬유공학회지 Vol.50 No.2

This study examined the abrasion and mechanical properties of poly(ethylene terephthalate) (PET) flameretardant fabrics used for window coverings. The tensile linearity, tensile resilience, bending rigidity, bending hysteresis, shearing stiffness, shearing hysteresis, compression energy, and surface roughness all increased with increasing fabric density and cover factor ratio. Five samples were subjected to more than 16,000 cycles for the abrasion resistance test and to grade 4 for the snagging test. The drape test showed that the flexibility of the samples was affected by fabric density and cover factor ratio.

물리치료학에서의 문제중심학습(Problem Based Learning)

이경희,김철용,김성학,Lee, Kyung-Hee,Kim, Chul-Yong,Kim, Seong-Hak 대한물리치료과학회 2002 대한물리치료과학회지 Vol.9 No.4

Problem based learning(PBL) is one of the learning strategies from the constructivism. It is a learning centered students. The tutors are facillitators as activators, helpers and cooperators not organizer in the classrooms. PBL makes that students learn creativity, independence, reasoning skits, communication and collaboration for problem solving. As the PBL process, students get the problems that are in real situation, discussed with others for brain storming, self directed study and revisited to the situation. They think critically and apply to the real situation. When students are to be physical therapists, they are easy to adopt their job and efficient to manage well. But inspite of a lot of advantages to them, there are much conflict to use as the learning strategies. Students perceived one of best learning method that they have experienced, but there are stress, burden, anxiety, timeless to prepare, lack of information and so on. PBL is effective to learning health oriented subjects, problem solving, even a lot preparation and processing for learning. It is reduced the differences between theories in colleges and practices in the fields. In processing of PBL, students get more many skills than the conventional learning. As trying many times to the classrooms, we can fixed to PBL with mistakes and conflict for better the development of the teaching and learning.

올란자핀의 체중증가 부작용 발생율 및 체중변화량

이경희,스리니바산샨무감,렌가라잔바스카란,산토쉬쿠마르나가야스리라만,용철순,최한곤,우종수,유봉규,Lee, Kyung-Hee,Shanmugam, Srinivasan,Baskaran, Rengarajan,Nagayya-Sriraman, Santhoshkumar,Yong, Chul-Soon,Choi, Han-Gon,Woo, Jong-Soo,Yoo, Bong-Kyu 대한약학회 2008 약학회지 Vol.52 No.4

Olanzapine, an atypical antipsychotic, has been widely used for the treatment of schizophrenia and bipolar disease. Although olanzapine is less associated with extrapyramidal symptoms and neuroleptic malignant syndrome compared to existing typical antipsychotics, the use of this drug has a problematic side effect of weight gain, which may cause metabolic syndrome such as type 2 diabetes. However, there are few hospitals practicing body weight monitoring of the patients on olanzapine or other atypical antipsychotics. The goal of this study was to identify the incidence and severity of weight gain associated with the use of the drug in Korea. We performed body weight monitoring of the patients who were on the drug in a hospital setting. Mean of the weight gain (as of one-month-transformation) was 4.33 and 3.39 kg for the male and female patients, respectively. The incidence in the young patients was higher than that observed in the old patients, and the severity was the highest in patients in their thirties followed by twenties or younger. This result suggests that the pattern of the weight gain associated with the use of olanzapine in Korea is similar to the reports performed and documented in US and European countries. Therefore, it appears that healthcare professionals in Korea should also watch on the weight gain issue in patients who are on olanzapine or other atypical antipsychotics.

중풍(中風) 후유증(後遺症)으로 발생된 배뇨장애(排尿障碍)에 대(對)한 구료법(灸療法)의 임상적(臨床的) 연구(硏究)

이경희,고형균,김창환,Lee, Kyung-Hee,Koh, Hyung-Kyun,Kim, Chang-Hwan 대한한의학회 1996 대한한의학회지 Vol.17 No.1

To study the clinical effect of moxibustion on the urinary disturbance, which is one of the most common complications of CVA, 60 patients with urinary disturbance due to CVA, who had been hospitalized in Hespital of Oriental Medicine, KyungHee University Medical Cental from May 1st, 1995, to September 20th, 1995, had been selected, and moxibustion treatment had been done. The results were as follows ; 1. In the relation between the radiological size of the lesion and recovery, the real moxibustion group showed significant recovery compared to sham moxibustion group and non moxibustion group in the middle size lesion patients. 2. In the relation between the anatomical lesion and recovery, there was no significant difference among real moxibustion group, sham moxibustion group and non moxibustion group. 3. In the relation between the initial state of urinary disturbance and recovery, the real moxibustion group showed significant recovery compared to sham moxibustion group and non moxibustion group in Gr 3 patients. 4. In the relation between the type of urinary disturbance and recovery, there was no significant difference among real moxibustion group, sham moxibustion group and non moxibustion group.

교통사고(交通事故) 환자(患者)의 한방치료(韓方治療)와 한양방협진치료(韓洋方協診治療) 비교(比較) 연구(硏究)

이경희,김정은,윤현민,고우신,송춘호,장경전,안창범,김철홍,Lee, Kyoung-Hee,Kim, Jung-Eun,Youn, Hyoun-Min,Ko, Woo-Shin,Song, Choon-Ho,Jang, Kyung-Jeon,Ahn, Chang-Beohm,Kim, Cheol-Hong 대한약침학회 2007 Journal of pharmacopuncture Vol.10 No.3

Objective The purpose of this study is to investigate the difference of treatment effect between Oriental Medicine therapy and Oriental and Western Medicine combination therapy on traffic accident patients. Methods Sixty one traffic accident patients were randomly assigned to the Oriental Medicine therapy group(group I)and Oriental and Western Medicine combination therapy group(group II). Evaluations were made before treatment, after one week treatment and after two weeks treatment using Visual Analog Scale(VAS), Oswestry disability Index(ODI), Neck Disability Index(NDI), Roland Morris Disability Scale(RMDS). The obtained data were analyzed and compared. Results The group I showed significant improvement(p<0.05) according to the VAS, NDI. But that showed insignificant improvement according to the ODI, RMDS. The group II showed significant improvement(p<0.05) according to the VAS, NDI. But that showed insignificant improvement according to the ODI, RMDS. And the difference between the two groups were insignificant according to VAS, NDI, ODI and RMDS. Conclusion There is no significant difference between the two groups after each therapy on traffic accident patients. Further studies are needed for the comparison of the Oriental Medicine therapy and Western Medicine combination therapy.

Some Properties of an RNA Ligase from Rhodosporidium toruloides

이경희,정연희,남정이,박인원,Lee, Kyung-Hee,Joung, Yean-Hee,NamShin, Jeong-E.,Park, In-Won Korean Society for Biochemistry and Molecular Biol 1987 한국생화학회지 Vol.20 No.2

Rhodosporidium toruloides의 무세포 추출물에서 새로운 RNA 연결효소의 활동성을 검출하였다. 이 효소계는 합성 UG의 5'-인산에 2'-, 3'-, 또는 고리형 2' : 3'-누클레오티드를 잇따라 결합시킴으로써 $N_nU_{16}G$(n= 1,2,3,...)을 합성시키는 것을 관찰하였다. 합성 올리고누클레오티드와의 연결반응은 2'-포스포모노에스테르, 3',5'-포스포디에스테르 중간체 결합의 형성에 의해서 진행한다. 이 RNA 연결효소는 그의 최대 활동성을 위해서 ATP와 $Mg^{2+}$을 필요로 한다. 흥미있는 것은, 무세포 추출물을 Micrococcus 에서 얻은 핵산가수분해효소로 처리하면 효소 활동성이 없어지지만, 데옥시리보 핵산가수분해효소로 처리한 경우에는 활동성에 영향을 미치지 않는다. 이것은 이 RNA 연결효소가 그의 연결작용에 RNA 성분을 필요로 함을 가리킨다. A novel RNA ligase activity has been detected in a cell-free extract from Rhodosporidium toruloides. The enzyme system was found to successively join 2'-, 3'-, or cyclic 2' : 3'-nucleotide to the 5'-phosphate of synthetic $U_{16}G$, leading to the synthesis of $N_{n}U_{16}G$(n=1,2,3...). The ligation of the nucleotides with synthetic oligonucleotide was found to proceed by forming 2'-phosphomonoester, 3',5'-phospho diester intermediate linkage. The RNA ligase requires ATP and $Mg^{2+}$ for its maximum activity. Interestingly, treatment of the cell- free extract with micrococcal nuclease but not with deoxyribonuclease abolishes the enzyme activity, indicating that the RNA ligase requires an RNA component(s) for its ligation activity.