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Porcine circovirus 2 국내 분리주의 유전적 특성
박최규,이경기,김현수,Park, Choi-Gyu,Lee, Kyoung-Ki,Kim, Hyun-Soo 대한수의학회 2004 大韓獸醫學會誌 Vol.44 No.4
In order to obtain the genetic informations of the Korean isolates of porcine circovirus 2 (PCV2), nucleotide sequences of total genome of three isolates and open reading frame 2 (ORF2) of four isolates were determined and compared with those of other reference PCV2 isolates. Nucleotide sequences of 3 isolates showed over 99% homology with those of reference strain (GenBank accession no. AF027217). Point mutations were mainly determined on ORF2 regions but little on ORF1 regions. The patterns of pointmutated sites and nucleotide substitution on ORF2 regions were generally consistent between Korean isolates, and these mutated sites observed in Korean isolates were also relatively similar to those of foreign isolates. Phylogenetic analysis of nucleotide or amino acid sequences showed that there were minor branches consisting of three clusters; cluster of Korea, Canada and America, cluster of Spain and Taiwan, and the last cluster of French and China isolates. These results suggested that Korean PCV2s were probably originated from North America such as Canada or USA. The genetic informations obtained from this study could be useful for the research of diagnosis and pathogenecity of PCV2.
이승희 ( Seunghee Lee ),정지열 ( Ji-youl Jung ),정재훈 ( Jae-hoon Jeong ),이현경 ( Hyunkyoung Lee ),박중원 ( Jung-won Park ),이경기 ( Kyoung-ki Lee ),김종완 ( Jong-hwan Kim ),소병재 ( Byungjae So ),윤순식 ( Soon-seek Yoon ) 한국동물위생학회(구 한국가축위생학회) 2018 韓國家畜衛生學會誌 Vol.41 No.3
Heat stroke in a dog is a life-threatening syndrome characterized by a high body temperature over 41°C, by the central nervous system dysfunction, and by multiple organ dysfunction. A 11-month-old male American Pit Bull Terrier was presented to clarify the cause of death to diagnostic laboratory. This dog showed clinical signs such as high body temperature (42°C), severe tachypnea, hematochezia, epistaxis and hemoptysis after transportation at hot summer time. At necropsy, there were hemorrhages in skin, serosa of stomach and small intestine, and also dark red fluid in lumen of intestine. Histopathologic examination revealed extensive hemorrhages in stomach, muscle, skin, and tongue. In microbiology, pathologic bacteria such as Pasteurella, Boedetella, Salmonella, Clostridium, and circulating virus in Korea such as CDV, CIV, CAV, CHV, CCV, CPIV were negative except CPV. There were many gross and microscopic lesions suggesting hest stroke while pathologic agents and related lesions were not detected. Therefore we diagnosed this case as heatstroke.
확률모형을 이용한 오제스키병 혈청학적 모니터링 프로그램 평가
장기윤,박선일,박최규,이경기,주이석,Chang, Ki-Yoon,Pak, Son-Il,Park, Choi-Kyu,Lee, Kyoung-Ki,Joo, Yi-Seok 대한수의학회 2009 大韓獸醫學會誌 Vol.49 No.2
The objective of this study was to analyze data from the planned national serological monitoring program for Aujeszky's disease (AD) using a simulation model to evaluate probable outcomes expected in the sample derived from the simulated herds at predefined within-herd prevalence and herd prevalence. Additionally, prevalence at animal- and herd-level estimated by the stochastic simulation model based on the distributions of the proportion of infected herds and test-positive animals was compared with those of data from a national serological survey in 2006, in which 106,762 fattening pigs from 5,325 herds were tested for AD using a commercial ELISA kit. A fixed value of 95% was used for test sensitivity, and the specificity was modeled with a minimum, most likely and maximum of 95, 97 and 99%, respectively. The within-herd prevalence and herd prevalence was modeled using Pert and Triang distributions, respectively with a minimum, most likely and maximum point values. In all calculations, population size of 1,000 was used due to lack of representative information. The mean number of infected herds and true test-positives was estimated to be 27 herds (median = 25; 95% percentile 44) and 214 pigs (median = 196; 95% percentile 423), respectively. When testing 20 pigs (mean of 2006 survey) in each herd, there was a 3.3% probability that the potential for false-positive reactions due to less than 100% specificity of the ELISA test would be detected. It was found that the model showed prevalence of 0.21% (99% percentile 0.50%) and 0.5% (99% percentile 0.99%) at animal- and herd-level, respectively. These rates were much similar to data from the 2006 survey (0.62% versus 0.83%). The overall mean herd-level sensitivity of the 2006 survey for fattening pigs was 99.9%, with only a 0.2% probability of failing to detect at least one infected herd.
혈청학적 분석을 통한 돼지 생식기호흡기증후군의 농장단위 감염유형
박최규,윤하정,이창희,정병열,이경기,김현수,Park, Choi-Kyu,Yoon, Ha-Chung,Lee, Chang-Hee,Jung, Byeong-Yeal,Lee, Kyoung-Ki,Kim, Hyun-Soo 대한수의학회 2008 大韓獸醫學會誌 Vol.48 No.1
Porcine reproductive and respiratory syndrome (PRRS) is the most economically important viral infectious disease in pig populations worldwide. This study was conducted to better understand the epidemic and dynamics of PRRS virus (PRRSV) on each farm and to evaluate the risk of PRRSV infection in Korea. Interviews with pig farmers were carried out to obtain PRRS vaccination programmes in 60 pig farms throughout Korea. Blood samples were also collected from the 59 pig farms to investigate outbreak patterns of each farm. Vaccination against PRRS was performed in 16.7% farms for breeding pigs and 8.3% of farms for nursery pigs. According to the seroepidemiological analysis, 56 (94.9%) out of 59 farms were considered to be affected by PRRSV infection. The results revealed that 68.9% of sows tested were seroconverted and interestingly, gilt herds had the highest seropositive rate (73%), suggesting that gilts may play a key role in PRRSV transmission in sow herds. Among the PRRS-affected piglet herds, 33 (55.9%), 14 (23.7%) and 6 (10.2%) farms were initially infected with PRRSV during the weaning, suckling and nursery period, respectively. It seems likely, therefore, that PRRSV infection predominantly occurs around the weaning period in piglet herds. Based on antibody seroprevalence levels in both sow and piglet groups, we were able to classify patterns of PRRSV infection per farm unit into 4 categories; category 1 (stable sow groups and non-infected piglet groups), category 2 (unstable sow groups and non-infected piglet groups), category 3 (stable sow groups and infected piglet groups), and category 4 (unstable sow groups and infected piglet groups). Our data suggested that 43 (72.9%) farms were analysed to belong to category 4, which is considered to be at high-risk for PRRS outbreak. Taken together, our information from this study will provide insight into the establishment of an effective control strategy for PRRS on the field.
이승희,정지열,정재훈,이현경,박중원,이경기,김종완,소병재,윤순식,Lee, Seunghee,Jung, Ji-Youl,Jeong, Jae-Hoon,Lee, Hyunkyoung,Park, Jung-Won,Lee, Kyoung-Ki,Kim, Jong-Hwan,So, ByungJae,Yoon, Soon-Seek 한국동물위생학회 2018 韓國家畜衛生學會誌 Vol.41 No.3
Heat stroke in a dog is a life-threatening syndrome characterized by a high body temperature over $41^{\circ}C$, by the central nervous system dysfunction, and by multiple organ dysfunction. A 11-month-old male American Pit Bull Terrier was presented to clarify the cause of death to diagnostic laboratory. This dog showed clinical signs such as high body temperature ($42^{\circ}C$), severe tachypnea, hematochezia, epistaxis and hemoptysis after transportation at hot summer time. At necropsy, there were hemorrhages in skin, serosa of stomach and small intestine, and also dark red fluid in lumen of intestine. Histopathologic examination revealed extensive hemorrhages in stomach, muscle, skin, and tongue. In microbiology, pathologic bacteria such as Pasteurella, Boedetella, Salmonella, Clostridium, and circulating virus in Korea such as CDV, CIV, CAV, CHV, CCV, CPIV were negative except CPV. There were many gross and microscopic lesions suggesting hest stroke while pathologic agents and related lesions were not detected. Therefore we diagnosed this case as heatstroke.
DNA 교차 오염 방지 기능을 가진 돼지 인플루엔자바이러스 감별진단용 one-step multiplex RT-PCR 진단법
김희정 ( Hee Jung Kim ),김은미 ( Eun Mi Kim ),신연경 ( Yeun Kyung Shin ),송재영 ( Jae Young Song ),김성희 ( Seong Hee Kim ),이경기 ( Kyoung Ki Lee ),이명헌 ( Myoung Heon Lee ),김영화 ( Young Hwa Kim ),박준철 ( Jun Cheol Park ) 한국동물위생학회 2014 韓國家畜衛生學會誌 Vol.37 No.4
In this study, we developed a cost and time saving one-step multiplex RT-PCR for the simultaneous detection and differentiation of swine influenza viruses (SIV) and 2009 pandemic influenza H1N1 virus (pH1N1). The one-step multiplex RT-PCR using four sets of primer was confirmed to be capable of detection of all SIV subtypes and differential diagnosis of major SIV subtype H1, H3 and pH1N1 on individual or mixed viral culture samples. The sensitivity of the multiplex RT-PCR was determined to be at least 2-6 HA/25 μL of the presented SIVs, providing sufficient efficacy for a routine SIV monitoring in diagnostic laboratories. In addition, compared with the conventional RT-PCR methods that cannot avoid the carryover DNA contamination, the developed RT-PCR applied with the uracil DNA glycosylase (UNG) system was proven to prevent a false positive reaction by carryover contamination of the pre-amplified DNA. In conclusion, the one-step RT-PCR with UNG system could be applicable to detect and differentiate of SIV from the viral cultures without worry of carryover DNA contamination in clinical laboratories.
정필수 ( Pil Soo Jeong ),박수진 ( Su Jin Park ),김은미 ( Eun Mi Kim ),박지영 ( Ji Young Park ),박유리 ( Yu Ri Park ),강대영 ( Dae Young Kang ),차현욱 ( Hyun Ouk Cha ),이경기 ( Kyoung Ki Lee ),김성희 ( Seong Hee Kim ),박최규 ( Choi 한국가축위생학회 2016 韓國家畜衛生學會誌 Vol.39 No.2
In the study, we developed and evaluated a uracil N-glycosylase (UNG)-supplemented single-tube nested reverse transcription-polymerase chain reaction (UsnRT-PCR) assay that can carried out first-round RT-PCR and second-round nested PCR in a reaction tube without reaction tube opening and can simultaneously detect EU- and NA-PRRSV. The UsnRT-PCR confirmed to have a preventing ability of mis-amplification by contamination of pre-amplified PRRSV DNA from previous UsnRT-PCR. Primer specificities were evaluated with RNAs extracted from 8 viral strains and our results revealed that the primers had a high specificity for both genotypes of PRRSV. The sensitivity of the UsnRT-PCR was 0.1 TCID50/0.1 mL for EU- or NA-PRRSV, respectively, which is comparable to that of previously reported real time RT-PCR (RRT-PCR). Clinical evaluation on 110 field samples (60 sera and 50 lung tissues) by the UsnRT-PCR and the RRT-PCR showed that detection rates of the UsnRT-PCR was 70% (77/110), and was relatively higher than that of the RRT-PCR (69.1%, 76/110). The percent positive or negative agreement of the UsnRT-PCR compared to RRT-PCR was 96.1% (73/76) or 90.9% (30/33), showing that the test results of both assays may be different for some clinical samples. Therefore, it is recommend that diagnostic laboratory workers use the two diagnostic assays for the correct diagnosis for the relevant samples in the swine disease diagnostic laboratories. In conclusion, the UsnRT-PCR assay can be applied for the rapid, and reliable diagnosis of PRRSV without concerns about preamplified DNA carryover contamination that can occurred in PCR process in the swine disease diagnostic laboratories.
이경기 대한감염학회 2010 감염과 화학요법 Vol.42 No.1
Since rabies virus infection is fatal for both human and animals, the protective immunization by vaccines against animals is a critical importance for disease prevention and control. In Korea, recurrence of sylvatic rabies was reported in Gangwon province, which is the northern border area in 1993. During the years of 1994-2009, 417 animal rabies cases were registered in Gangwon and Gyeonggi provinces. The national animal rabies eradication program has performed to reduce rabies outbreaks by annual vaccination of dogs and cattle since 1995, and the oral vaccination of raccoon dogs with annual vaccinia-rabies glycoprotein (V-RG) bait vaccine. Additionally, serological surveillance on domestic animals has been executed since 1999 to monitor the anti-rabies antibody titers from Gangwon and Gyeonggi provinces where the vaccination campaign has been implemented.
소병재,진영화,이상직,이은동,이경기,황의경,이상경,김재훈,박최규,윤순식,김기석,문운경,신명균,이성식 한국수의공중보건학회 2002 예방수의학회지 Vol.26 No.2
This study was conducted to investigate the current status of rabies in wild animals and to elucidate the wild animal transmitting rabies to domestic animals in the Republic of Korea. Through epidemiological survey of rabies near the Demilitarized Zone(DMZ) in Kyonggi and Kangwon Provinces, we found that raccoon dogs( Nyctereutes procyonoides) transmitted rabies to domestic animals in the infected area.To do this we tested the sera or/and brain tissue collected from 8 kinds of wild animals( Nyctereutes procyonoides, Meles meles, Lepus coreanus, Apodemus agrarius, Eothenomys regulus, Felis catus, Mutela sibirica coreana, Martes flavigula aterrima). Only raccoon dogs showed positive reaction in fluorescent antibody test. Naturally acquired antibodies against rabies virus could not be detected in other animals as well as raccoon dogs captured in the rabies infected and non-infected area in Korea. Antibodies against rabies virus could not be detected even raccoon dogs diagnosed with rabies. Therefore, it is considered that raccoon dogs would die before forming antibodies against rabies virus, if infected.