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간암 세포주 HepG2에 대한 맥문동탕(麥門冬湯)추출물의 항암 및 항전이 효능
전명숙 ( Myeong Sook Cheon ),천진미 ( Jin Mi Chun ),윤태숙 ( Tae Sook Yoon ),이아영 ( A Yeong Lee ),문병철 ( Byeong Cheol Moon ),추병길 ( Byung Kil Choo ),김성환 ( Seong Hwan Kim ),김호경 ( Ho Kyoung Kim ) 대한본초학회 2009 大韓本草學會誌 Vol.24 No.3
Objectives: Maekmoondong-tang (MMDT), a Korean herbal medicine, has been used to treat severe dry cough in patients with bronchitis and pharyngitis. MMDT has been reported to have anti-inflammatory, anti-allergic, immunomodulatory, secretory-modulating, and metabolic regulatory actions. However, there are no evidence in regard to the effects of MMDT on carcinogenesis and metastasis. Here, we investigated the effects of 70% ethanol extract of MMDT on cell viability, apoptosis, and motility in human hepatocarcinoma HepG2 cells. Methods: Cell viability was measured using the CCK-8 assay, and the apoptosis induction was evaluated by caspase-3 activity. To detect apoptotic features, the cells treated with MMDT were stained with 4`-6-diamidino-2-phenylindole (DAPI). Cell motility was examined by Boyden chamber assay and Real-time Cell Index of Migration assay. Gelatin zymography also performed to measure matrix metalloproteinase (MMP)-2/9 activity. Results: We found that MMDT significantly inhibited cell proliferation and increased caspase-3 activity in a dose-dependent manner in HepG2 cells. Apoptotic features such as chromatin condensation and apoptotic bodies were observed in MMDT-treated cells by DAPI staining. MMDT also suppressed PMA-induced cell motility and activities of MMP-2/9. Conclusions: Our results exhibited that MMDT possess the anti-carcinogenetic and anti-metastatic activities via caspase-3 activation and down-regulation of cell motility and invasion in HepG2 cells. Therefore, these findings suggest that MMDT could be potentially applied to the prevention and treatment of cancer.
이도연 ( Do Yeon Lee ),최고야 ( Go Ya Choi ),윤태숙 ( Tae Sook Yoon ),전명숙 ( Myeong Sook Cheon ),김승주 ( Seung Ju Kim ),야스카와켄 ( Ken Yasukawa ),유소연 ( So Yeon Yu ),김호경 ( Ho Kyoung Kim ) 대한본초학회 2008 大韓本草學會誌 Vol.23 No.4
Objectives: Notopterygium incisum (N. incisum) and Saposhnikovia divaricata (S. divaricata) have been clinically used in traditional oriental medicine for treatment of inflammatory diseases. Also, a herbal mixture prepared with N. incisum and S. divaricata has been strongly linked to the anti-inflammatory effect. In this study, we evaluate the synergistic anti-inflammatory effect of N. incisum and S. divaricata. Methods: For evaluating the anti-inflammatory activity of a herbal mixture of N. in incisum and S. divaricata in vivo, we measured the changed ear thickness in 12-O-tetradecanoyl-phorbol-13-acetate (TPA)-induced mouse ear edema model after topical application of herbal mixture. In addition, the levels of markers for inflammation, such as tumore necrosis factor (TNF)-α, interleukin (IL)-1β, and nitric oxide (NO), were determined by ELISA assay in lipopolysaccharide-stimulated Raw 264.7 cells. Results: We reported that water extracts of N. incisum and S. divaricata combination significantly inhibited the mouse ear edema induced by TPA. Moreover, the water extracts of N. incisum and S. divaricata combination exhibited synergistic effects in down-regulating IL-1β level, but not TNF-α and NO. Conclusions: These results suggest that combined treatment of N. incisum and S. divaricata, based on seven methods in prescription compatibility, has a synergistic effect in down-regulating inflammatory response both in vivo and in vitro models. Especially, it seems that IL-1β is a one of main target of the mixture of N. incisum and S. divaricata on anti-inflammatory activity.
rDNA-ITS 염기서열 분석을 통한 시호 종 감별용 유전자 마커 개발 및 유연관계 분석
문병철 ( Byeong Cheol Moon ),추병길 ( Byeong Kil Choo ),지윤의 ( Yun I Ji ),윤태숙 ( Tae Sook Yoon ),이아영 ( A Young Lee ),전명숙 ( Myeong Sook Cheon ),김보배 ( Bo Bae Kim ),김호경 ( Ho Kyoung Kim ) 대한본초학회 2009 大韓本草學會誌 Vol.24 No.3
Objectives: Bupleuri Radix (Siho) is prescribed as the root of different Bupleurum species on the pharmarcopoeia in Korea and China. Moreover, other species and varieties of the genus Bupleurum have been also distributed on the herbal market as Bupleuri Radix. However, due to the morphological similarity and frequent occurrence of intermediate forms, the correct identification of this radix is very difficult. To develop a reliable method for correct identification and improving the quality standards of official Bupleuri Radix, we analyzed sequences of the ribosomal RNA gene and internal transcribed spacer (rDNA-ITS) region. Methods: PCR amplification of rDNA-ITS region was performed using ITS1 and ITS4 primer from 6 Bupleurum species and 1 variety, B. falcatum L. (Siho), an improved breed of B. falcatum L. (Samdo-Siho), B. chinense DC. (Buk-Siho), B. scorzonerifolium Willd. (Nam-Siho), B. longiadiatum Turcz. (Gae-Siho), B. euphorbiodes Nakai (Deungdae-Siho) and B. latissimum Nakai (Seom-Siho), and nucleotide sequence was determined after sub-cloning into the pGEM-Teasy vector. Authentic marker nucleotides were estimated by the analysis of ClastalW using entire rDNA-ITS sequence of three samples per species. Results: In comparative analysis of the rDNA-ITS sequences, we found specific nucleotides to distinguish Korean (B. falcatum L. and its variety) and Chinese official species (B. chinense DC. and B. scorzonerifolium Willd.) from others at positions 411 and 447, and positions 89, 101, 415 and 599, respectively. Futhermore, we also found nucleotide indels (insertion and/or deletion) and substitutions to identify each of different Bupleurum species, 2 positions for B. falcatum L. and its variety, 6 positions for B. chinense DC., 49 positions for B. scorzonerifolium Willd., 8 positions for B. euphorbioides Nakai, 7 positions for B. longiradiatum Nakai and 9 positions for B. latissimum Nakai. These sequence differences at corresponding positions are avaliable nucleotide markers to determine the botanical origins of Bupleuri Radix. Moreover, we confirmed the phylogenetic relationship of B. latissimum Nakai, a Korean endemic speices, among Bupleurum species based on the rDNA-ITS sequence. Conclusions: These marker nucleotides would be useful to identify the official herbal medicines by the providing of definitive information that can identify each plant species and distinguish it from unauthentic adulterant Bupleurum species.
양원경 ( Won Kyung Yang ),김호경 ( Ho Kyoung Kim ),성윤영 ( Yoon Young Sung ),전명숙 ( Myeong Sook Cheon ),윤태숙 ( Tae Sook Yoon ) 대한본초학회 2010 大韓本草學會誌 Vol.25 No.4
Objective: The purpose of this study is to compare anti-thrombotic activities of 4 Rhubarb species. Methods: Rhubarb has been used as medical and edible resources worldwide for the therapy of cancer, constipation and inflammation, etc. To compare the anti-thrombotic activities among the species within rhubarb, we investigated and compared the inhibitory properties of water extracts from R. palmatum(RPE), R. officinale(ROE), R. nobile(RNE), and R. franzenbachii (RFE) on the fibrinolysis, blood coagulation and platelet aggregation. APTT and PT, blood coagulation time, were measured by apparatus of auto blood coagulation analysis that called ACL-7000. The measurement of fibrinolysis was measured and compares with the width of clear zone from melted fibrin plate. The platelet aggregation was measured and compared with inhibition rate of aggregation. Results: We found out the best species from rhubarb showing the significant fibrinolytic, anti-coagulant and anti-platelet aggregation activity. Our results show that Rheum nobile (RNE) has the most effective anti-thrombotic activity among 4 tested rhubarb. Conclusions: This study, therefore, RNE will be able to be used the development of drug which is thrombosis treatment.
외대잔대(Adenophora raoemosa J.Lee & S.Lee)개체군의 classification과 ordination 분석
지윤의 ( Yun Ui Ji ),문병철 ( Byeong Cheol Moon ),이야영 ( A Yeong Lee ),윤태숙 ( Tae Sook Yoon ),송호경 ( Ho Kyung Song ),추병길 ( Byung Kil Choo ),김호경 ( Ho Kyoung Kim ) 한국환경보건기술학회(구 한국환경복원녹화기술학회) 2009 한국환경복원기술학회지 Vol.12 No.6
This study was carried out to investigate vegetation structure and soil properties of Adenophora racemosa population distributed in Jeombongsan, Seoraksan, Odaesan and Dutasan in Gangwon-do, Korea. From August 2007 until September 2009, 2 m × 2 m quadrate was established in native area of Adenophora racemosa in order to record a dominants and coverage, and soil factors at 22 sites. It was found that the altitude in the distributed areas for Angelica gigas population was 800 m or more. Adenophora racemosa population was classified into Rhododendron mucronulatum dominant population, Geranium var. hirsutum dominant population, Carex siderosticta dominant population and Aruncus dioicus var. kamtschaticus dominant population. In the site of study, soil pH, electrical conductivity, soil organic matter, available phosphorous, and exchangeable potassimn, exchangeable calcimn, exchangeable magnesium, exchangeable sodium concentration and total nitrogen were ranged from 5.0~7.1, 0.06~0.65dS/m, 0.96~8.94%, 12.3~32.8mg/kg, 0.12~0.89cmol+/kg, 0.34~10.08cmol+/kg, 0.1~l.4cmol+/kg, 0.02~0.29cmol+/kg and 0~8.4% respectively. According to the results of DCCA, Adenophora racemosa population were distributed in the high available phosphorous and exchangeable potassium, and Rhododendron mucronulatum dominant population was situated on low available potassium and high slope degree, Geranium var. hirsutum dominant population was high altitude, electrical conductivity and exchange magnesium, and Carex siderosticta dominant population and Aruncus dioicus var. kamtschaticus were distributed on high total nitrogen, organic matter, available phosphorous.