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B16/F10 흑색종양세포에서 삼내자 메탄올 추출물의 멜라닌 생성에 미치는 억제효과
윤정원,한정민,윤화정,고우신,Yoon, Jung-Won,Han, Jung-Min,Yoon, Hwa-Jung,Ko, Woo-Shin 대한한방안이비인후피부과학회 2013 한방안이비인후피부과학회지 Vol.26 No.1
Objective: Recently the demands for the effective and safe depigmentative and anti-aging agents of the skin have increased due to the medical, pharmaceutical and cosmetic reasons. The purpose of this study is to investigate the MKG(Methanol Extract of Kaempferia galanga) and their dermal bioactivity properties related to cosmeceuticals such as depigmentation. Methods: We assessed inhibitory effects of MKG on melanin production in B16/F10 melanoma cells, on mushroom tyrosinase activity, effects of MKG on the expression tyrosinase, TRP-1, TRP-2, GSK-$3{\beta}$, CREB, MITF in B16/F10 melanoma cells without cytotoxicity range. Cell viability was measured by MTT assay and tyrosinase activity was assessed using by DOPA staining, western-blot analysis. We measured inhibition of melanin synthesis and tyrosinase activity by down-regulation of melanogenic enzyme expressions in ${\alpha}$-MSH induced melanogenesis B16/F10 melanoma cells. Results: MKG inhibited tyrosinase-activity, total melanin contents and dendrite out-growth. MKG inhibited melanogenesis by down-regulation of tyorsinase, TRP-1, TRP-2, CREB, and MITF in B16/F10 cells. The treatment with MKG at the 12.5, $25{\mu}g/ml$ level significantly inhibited the melanin synthesis induced ${\alpha}$-MSH in B16/F10 melanoma cells compared with untreated control. Conclusion: These results suggest that MKG inhibit melanin biosynthesis which is involved in hyper-pigmentation. So MKG is considered to be used as a whitening components reducing cytotoxicity.
윤정원,방정환,고용호,유세훈,김준기,이창우,Yoon, Jeong-Won,Bang, Jung-Hwan,Ko, Yong-Ho,Yoo, Se-Hoon,Kim, Jun-Ki,Lee, Chang-Woo 한국마이크로전자및패키징학회 2014 마이크로전자 및 패키징학회지 Vol.21 No.4
The paper gives an overview of the concepts, basic requirements, and trends regarding packaging technologies of power modules in hybrid (HEV) and electric vehicles (EV). Power electronics is gaining more and more importance in the automotive sector due to the slow but steady progress of introducing partially or even fully electric powered vehicles. The demands for power electronic devices and systems are manifold, and concerns besides aspects such as energy efficiency, cooling and costs especially robustness and lifetime issues. Higher operation temperatures and the current density increase of new IGBT (Insulated Gate Bipolar Transistor) generations make it more and more complicated to meet the quality requirements for power electronic modules. Especially the increasing heat dissipation inside the silicon (Si) leads to maximum operation temperatures of nearly $200^{\circ}C$. As a result new packaging technologies are needed to face the demands of power modules in the future. Wide-band gap (WBG) semiconductors such as silicon carbide (SiC) or gallium nitride (GaN) have the potential to considerably enhance the energy efficiency and to reduce the weight of power electronic systems in EVs due to their improved electrical and thermal properties in comparison to Si based solutions. In this paper, we will introduce various package materials, advanced packaging technologies, heat dissipation and thermal management of advanced power modules with extended reliability for EV applications. In addition, SiC and GaN based WBG power modules will be introduced.
윤화정,윤정원,윤소원,고우신,우원홍,Yoon, Hwa-Jung,Yoon, Jung-Won,Yoon, So-Won,Ko, Woo-Shin,Woo, Won-Hong 대한한방안이비인후피부과학회 2003 한방안이비인후피부과학회지 Vol.16 No.1
Recently many efforts were focused to understand the mechanical insights of melanogenesis to develop the agents for hyper-pigmentation and hypo-pigmentation. In the melanin biosynthetic pathway, tyrosinase is the rate limiting enzyme, and ${\alpha}$-melanocyte stimulating hormone(MSH) or cAMP-elevating agents stimulate melanogenesis and enhance the melanin synthesis and the tyrosinase activity. The author has analyzed the effects of Rhizoma Bletillae on the basal melanogenic activities of B16 mouse melanoma cells. Rhizoma Bletillae alone markedly suppressed melanin content and tyrosinase activity in a dose-dependent manner. Pretreatment of the cells with Rhizoma Bletillae. The decrease in the tyrosinase activity was paralled by a decrease in the abundance of tyrosinase protein and tyrosinase promoter activity. These results suggest that Rhizoma Bletillae inhibits melanogenesis of B16 melanoma cells via suppression of tyrosinase activity.
白급이 B16 흑색종세포의 멜라닌 형성 억제에 미치는 영향
윤화정,윤정원,윤소원,고우신,우원홍,Yoon, Hwa-jung,Yoon, Jung-won,Yoon, So-won,Ko, Woo-shin,Woo, Won-hong 대한한방안이비인후피부과학회 2003 한방안이비인후피부과학회지 Vol.16 No.3
Recently many efforts were focused to understand the mechanical insights of melanogenesis to develop the agents for hyper-pigmentation and hypo-pigmentation. In the melanin biosynthetic pathway, tyrosinase is the rate limiting enzyme, and ${\alpha}$-melanocyte stimulating hormone(MSH) or cAMP-elevating agents stimulate melanogenesis and enhance the melanin synthesis and the tyrosinase activity. The author has analyzed the effects of Rhizoma Bletillae on the basal melanogenic activities of B16/F10 mouse melanoma cells, and on the ${\alpha}$-MSH or forskolin-induced melanogenesis. Rhizoma Bletillae alone markedly suppressed melanin content and tyrosinase activity in a dose-dependent manner. Pretreatment of the cells with Rhizoma Bletillae also suppressed the increase of ${\alpha}$-MSH (100 nM) or forskolin (20 ${\mu}M$)-induced melanin content and tyrosinase activity. The decrease in the tyrosinase activity was paralled by a decrease in the abundance of tyrosinase protein and tyrosinase promoter activity. Pretreatment of the cells with Rhizoma Bletillae also inhibited the increase of forskolin(20${\mu}M$) induced the amount of tyrosinase protein and tyrosinase promoter activity. The results of DOPA staining revealed that pretreatment of the cells with Rhizoma Bletillae showed less intensity than B16 melanoma cells stimulated with ${\alpha}$-MSH or forskolin. These results suggest that Rhizoma Bletillae inhibits melanogenesis and abrogates ${\alpha}$-MSH and cAMP-induced melanogenesis in B16 melanoma cells.
論文(논문) : 항공안전인증을 고려한 소형제트항공기 플랩 재설계
윤정원 ( Jung Won Yoon ),이효진 ( Hyo Jin Lee ),이재우 ( Jae Woo Lee ),김상호 ( Sang Ho Kim ),변영환 ( Yung Hwan Byun ),김임권 ( Im Gun Kim ) 한국항공운항학회 2011 한국항공운항학회지 Vol.19 No.3
In this paper, a conceptual design process for Very Light Jet aircraft has been proposed incorporating aircraft safety certification. During the proposed design process, satisfaction of the airworthiness certification for an intermediate resulting aircraft configuration is evaluated and then redesigns are carried out if necessary and until the designed aircraft configuration satisfies the airworthiness requirements. Certification database has been developed using FAR 23, AC 23, KAS 23, and CS 23 as the airworthiness certification. Based on the developed certification database Design Certifcation Related Table has been produced to use the airworthiness requirements as design constraints in the propsed design process. Using Quality Function Deployment the design variables for a redesign are carefully selected and a design optimization is performed. To demonstrate the feasibility and effectiveness of rapid aircraft conceptual design using the proposed approach, a Very Light Jet design optimization including a redesign of wing flap has been performed and the design results have been presented.
매선요법(埋線療法)을 이용한 중증 구안괘사(口眼?斜) 초기치료에 대한 임상적 고찰
한정민,윤정원,강나루,고우신,윤화정,Han, Jung-Min,Yoon, Jung-Won,Kang, Na-Ru,Ko, Woo-Shin,Yoon, Hwa-Jung 대한한방안이비인후피부과학회 2012 한방안이비인후피부과학회지 Vol.25 No.3
Objectives : This study was performed to investigate the effect of Needle-Embedding Therapy in early stage of intractable peripheral facial paralysis. Methods : 46 patients with severe peripheral facial paralysis were treated with oriental medicine therapy including Needle-Embedding Therapy in their early stage. We evaluated the effect of Needle-Embedding Therapy by House-Brackmann Grading system, decrease of subjective symptoms and satisfaction measurement. Results : After treatment, HB-Scale grade was significantly decreased(p-value<0.001). More than 50% of subjective symptoms were disappeared in 82.61% of patients. 80.48% of the patients was satisfied with the Needle-Embedding Therapy. Conclusion : Needle-Embedding Therapy could be effective to improve symptoms of severe peripheral facial paralysis in early stage. Further studies will be required to identify the beneficial effect of Needle-Embedding Therapy in early stage of peripheral facial paralysis.
방정환(Jung-Hwan Bang),유동열(Dong-Yurl Yu),고용호(Young-Ho Ko),윤정원(Jeong-Won Yoon),이창우(Chang-Woo Lee) 대한용접·접합학회 2016 대한용접·접합학회지 Vol.34 No.1
Automotive today has been transforming to an electronic product by adopting a lot of convenience and safety features, suggesting that joining materials and their mechanical reliabilities are getting more important. In this study, a Sn-Cu-Cr-Ca solder composition having a high melting temperature (>230℃) was fabricated and its joint properties and reliability was investigated with an aim to evaluate the suitability as a joining material for electronics of engine room. Furthermore, mechanical properties change under complex environment were compared with several existing solder compositions. As a result of contact angle measurement, favorable spreadability of 84% was shown and the average shear strength manufactured with corresponding composition solder paste was 1.9kg/mm². Also, thermo-mechanical reliability by thermal shock and vibration test was compared with that of the representative high temperature solder materials such as Sn-3.5Ag, Sn-0.7Cu, and Sn-5.0Sb. In order to fabricate the test module, solder balls were made in joints with ENIG-finished BGA and then the BGA chip was reflowed on the OPS-finished PCB pattern. During the environmental tests, resistance change was continuously monitored and the joint strength was examined after tests. Sn-3.5Ag alloy exhibited the biggest degradation rate in resistance and shear stress and Sn-0.7Cu resulted in a relatively stable reliability against thermo-mechanical stress coming from thermal shock and vibration.