B16/F10 흑색종양세포에서 삼내자 메탄올 추출물의 멜라닌 생성에 미치는 억제효과

윤정원,한정민,윤화정,고우신,Yoon, Jung-Won,Han, Jung-Min,Yoon, Hwa-Jung,Ko, Woo-Shin 대한한방안이비인후피부과학회 2013 한방안이비인후피부과학회지 Vol.26 No.1

Objective: Recently the demands for the effective and safe depigmentative and anti-aging agents of the skin have increased due to the medical, pharmaceutical and cosmetic reasons. The purpose of this study is to investigate the MKG(Methanol Extract of Kaempferia galanga) and their dermal bioactivity properties related to cosmeceuticals such as depigmentation. Methods: We assessed inhibitory effects of MKG on melanin production in B16/F10 melanoma cells, on mushroom tyrosinase activity, effects of MKG on the expression tyrosinase, TRP-1, TRP-2, GSK-$3{\beta}$, CREB, MITF in B16/F10 melanoma cells without cytotoxicity range. Cell viability was measured by MTT assay and tyrosinase activity was assessed using by DOPA staining, western-blot analysis. We measured inhibition of melanin synthesis and tyrosinase activity by down-regulation of melanogenic enzyme expressions in ${\alpha}$-MSH induced melanogenesis B16/F10 melanoma cells. Results: MKG inhibited tyrosinase-activity, total melanin contents and dendrite out-growth. MKG inhibited melanogenesis by down-regulation of tyorsinase, TRP-1, TRP-2, CREB, and MITF in B16/F10 cells. The treatment with MKG at the 12.5, $25{\mu}g/ml$ level significantly inhibited the melanin synthesis induced ${\alpha}$-MSH in B16/F10 melanoma cells compared with untreated control. Conclusion: These results suggest that MKG inhibit melanin biosynthesis which is involved in hyper-pigmentation. So MKG is considered to be used as a whitening components reducing cytotoxicity.

전기자동차용 고신뢰성 파워모듈 패키징 기술

윤정원,방정환,고용호,유세훈,김준기,이창우,Yoon, Jeong-Won,Bang, Jung-Hwan,Ko, Yong-Ho,Yoo, Se-Hoon,Kim, Jun-Ki,Lee, Chang-Woo 한국마이크로전자및패키징학회 2014 마이크로전자 및 패키징학회지 Vol.21 No.4

The paper gives an overview of the concepts, basic requirements, and trends regarding packaging technologies of power modules in hybrid (HEV) and electric vehicles (EV). Power electronics is gaining more and more importance in the automotive sector due to the slow but steady progress of introducing partially or even fully electric powered vehicles. The demands for power electronic devices and systems are manifold, and concerns besides aspects such as energy efficiency, cooling and costs especially robustness and lifetime issues. Higher operation temperatures and the current density increase of new IGBT (Insulated Gate Bipolar Transistor) generations make it more and more complicated to meet the quality requirements for power electronic modules. Especially the increasing heat dissipation inside the silicon (Si) leads to maximum operation temperatures of nearly $200^{\circ}C$. As a result new packaging technologies are needed to face the demands of power modules in the future. Wide-band gap (WBG) semiconductors such as silicon carbide (SiC) or gallium nitride (GaN) have the potential to considerably enhance the energy efficiency and to reduce the weight of power electronic systems in EVs due to their improved electrical and thermal properties in comparison to Si based solutions. In this paper, we will introduce various package materials, advanced packaging technologies, heat dissipation and thermal management of advanced power modules with extended reliability for EV applications. In addition, SiC and GaN based WBG power modules will be introduced.

Optoelectronic 패키징을 위한 Au-Sn 플립칩 범핑 기술과 신뢰성

윤정원,김종웅,구자명,노보인,정승부,Yoon, Jeong-Won,Kim, Jong-Woong,Koo, Ja-Myeong,Noh, Bo-In,Jung, Seung-Boo 대한용접접합학회 2008 대한용접·접합학회지 Vol.26 No.1

전자 패키징의 플립칩 본딩 기술과 신뢰성

윤정원,김종웅,구자명,하상수,노보인,문원철,문정훈,정승부,Yoon, Jeong-Won,Kim, Jong-Woong,Koo, Ja-Myeong,Ha, Sang-Su,Noh, Bo-In,Moon, Won-Chul,Moon, Jeong-Hoon,Jung, Seung-Boo 대한용접접합학회 2007 대한용접·접합학회지 Vol.25 No.2

BALB/c 마우스에서 동종 지방유래 기질세포가 창상치유에 미치는 영향

윤정원,임진수,김정남,유결,Yoon, Jeong-Won,Lim, Jin-Soo,Kim, Jung-Nam,Yoo, Gyeol 대한성형외과학회 2010 Archives of Plastic Surgery Vol.37 No.4

Purpose: Adipose-derived stromal cells (ADSCs) are multipotent cells that have been found to promote wound healing through the process of angiogenesis and reepithelialization. Generally, it is well known that the antigenicity of ADSCs doesn't affect stem cell therapy. In this study, we investigated the effect of allogeneic ADSCs in the wound healing process by applying allogeneic ADSCs on the wound healing splint model of mice. Methods: Adipose tissue was harvested from the epididymal fat pads of BALB/c and C57BL/6 mice. Twenty four mice BALB/c were divided into three groups; control, isogeneic, and allogeneic groups. Two full thickness defects with 6 mm diameters were created on the back of BALB/c mice. $1{\times}10^6$ ADSCs from BALB/c mice were applied on the isogeneic group. In the allogeneic group, ADSCs from the C57BL/6 mice were applied. No cells were applied to the control group. The sizes of the wounds were evaluated in 3, 5, 7, 10, and 14 days after the wounds were applied, and tissues were harvested in 7 and 14 days for histological analysis. Results: Wound healing rates had showed significant increase in 10, and 14 days when the isogeneic group was compared to the control group, but the allogeneic group showed significantly decrease compared to the isogeneic group (p<0.05). Histological scores in the isogeneic group were significantly high, but significantly lower in the allogeneic group when compared to the isogeneic group in 2 weeks (p<0.05). In the isogeneic group, thick inflammatory cell infiltration with abundant capillaries were observed in 1 week, and thick epithelium with many large capillaries were observed in 2 weeks. Conclusion: When isogeneic ADSCs were applied to wounds, they presented a faster wound healing rate compared to controls and the allogeneic group. Unlike general stem cell therapy, these findings suggest that cell therapy targeted at enhancing wound healing may benefit from the use of ADSCs with identical antigenicity, as opposed to allogeneic or xenogenic ADSCs.

전자 패키징의 요소기술

윤정원,문원철,정승부,Yoon Jeong-Won,Moon Won-Chul,Jung Seung-Boo 대한용접접합학회 2005 대한용접·접합학회지 Vol.23 No.2

임상간호사의 주관적 삶의 질 예측모형

윤정원(Yoon, Jung Won),박영숙(Park, Yeong Sook) 계명대학교 간호과학연구소 2013 계명간호과학 Vol.17 No.1

Purpose: The purpose of this study was to test the direct or indirect effect of valuables that could explain the subjective quality of life of clinical nurses and to suggest a proedictive model. Method: Data was collected through the self-reporting questionnaires from 437 clinical nurses working in the general hospitals located in D city. Result: The result showed that family income level, support of boss and co-workers, work stress, self-esteem, and job satisfaction were found to have significant direct effect on subjective quality of life. Indirect factors were age, shift work, and health status. The modified model was concise and extensive in predicting subjective quality of life of the participants. The final modified model yielded GFI=.99, AGFI=.96, RMR=.04, NF.98, TLI=.97, and CFI=.99 and exhibited good fit indices. Conclusion: These findings of this study may contribute to development of a program for better understanding and further improvement of the subjective quality of life of clinical nurses.

경주국립공원 문화재지정구역의 귀화식물 현황 및 관리방안

윤정원 ( Jung Won Yoon ),신현탁 ( Hyun Tak Shin ),이명훈 ( Myung Hoon Yi ),김기송 ( Gi Song Kim ),성정원 ( Jung Won Sung ),박기환 ( Ki Hwan Park ),이창현 ( Chang Hyun Lee ),김용식 ( Yong Shik Kim ) 한국환경생태학회 2012 한국환경생태학회지 Vol.26 No.4

경주국립공원 문화재지정구역에 분포하는 귀화식물은 총 14과 40속 45종 1변종 등 총 46분류군을 확인하였다. 귀화식물을 유형별로 구별해보면, 생활형은 1년생 초본이 17분류군(37%)으로 가장 많았으며, 다년생 초본이 16분류군 (34.8%), 2년생 초본이 11분류군(23.9%), 목본이 2분류군(4.3%)로 나타났다. 원산지 별로는 유럽이 17분류군(37%)으로 가장 많았으며, 그 다음으로 북아메리카에서 이입된 종이 16분류군(34.8%)으로 나타났다. 또한 귀화도는 3등급이 18분류군(40.9%)으로 가장 높다. 귀화식물 321분류군에 대한 본 연구대상지의 도시화 지수는 14.3%이며, 지구별 도시화 지수는 남산지구가 8.1%로 가장 높다. 경주국립공원은 사적형 공원으로 문화재가 매우 중요하나, 문화재 주변의식재 및 관리 또한 중요하다. 따라서 귀화식물의 경우 장기적인 모니터링과 생태적인 특성을 파악하여 체계적인 관리방안이 요구된다. The naturalized plant species in Gyeongju National Park was summarized as 46taxa including 14 families 40 genus 45 species 1 varieties. Among them, annual herbaceous species was 17taxa (37%) which is grouped as the biggest proportion and 16taxa (34.8%) for perennial herbaceous species and 11taxa (23.9%) for biannual herbaceous species and 2taxa (4.3%) for tree species. European origin was the biggest proportion as 17taxa (37%), and 16% (34.8%) from North America. The degree of naturalization (DoN) was the biggest for Degree III, V as 18taxa (39.1%). Regarding 321 naturalized plant species, the Urbanization Index in the studied area was 14.3%. The Namsam District showed 8.1% which is the highest DoN in the studied districts within the Gyeongju National Park. As the cultural properties is important in the Gyeongju National Park as a representatives for historical sites in Korea, planting and its management in the cultural properties is important tasks. The naturalized plant species in the studied area should be reasonably management.

일정 관리에서 캘린더 사용 특성에 관한 연구

윤정원(Jung-Won Yoon) 한국HCI학회 2016 한국HCI학회 학술대회 Vol.2016 No.1

모바일 미디어는 시공간성에 큰 변화를 불러오고 있다. 예를 들어, 스마트폰의 LBS, GPS 는 물리적으로 길을 찾는 과정없이도 공간을 경험하게 했으며, 하이퍼미디어를 통한 이동 또한 우리의 공간 인식을 변화시켰다. 그렇다면, 모바일이 우리의 시간성에는 어떠한 변화를 가져왔을까? 모바일 미디어에서는 ‘과거’로의 접근과 현재성(Now-ness)을 강화시키는 타임라인 양식이 지배적인 인터페이스 형식으로 자리잡았지만, 예측불가능한 미래를 표현하는 인터페이스는 통용되는 양식이 없어, 모바일 캘린더는 전통적인 양식을 그대로 사용하고 있다. 한편, 모바일은 개인의 시간 주도권을 회복시켰고, 개인화된 시간을 만든다. 그러나 이는 협업시, 개인화된 시간을 사회적 시간과 동기화하거나 일정을 맞추는 것을 더욱 어렵게 만들었다. 그러므로, 개인이 주도권을 갖는 시간에서 사회적 시간성을 증가시킬 수 있는 대안이 필요하다. 본 연구는 모바일에서 ‘미래’를 나타낼 수 있는 양식과 일정의 사회성을 관리하는 방안을 모색하고자, 모바일 캘린더의 사용 행태를 알아볼 것이다. 이를 위해, 사용하는 캘린더를 수집하여, 사람들의 일정 관리 및 사회성 관리 전략을 도출하고자 한다. Mobile media brings about a great change to spatio-temporal. In the situation that LBS and GPS in Smartphone caused the change about our ‘spatial perception’, how did Smartphone cause ‘temporal perception’? In mobile media, the timeline style that strengthens the approach to the past and ‘now-ness’ settled as a dominant interface type. However, since the interface that represents the unpredictable future does not have current form in mobile calendar, it could not help using traditional form until now. In the case of Smartphone, while it recovered the initiative for individual time management, it made people harder about synchronizing personalized and socializing time. So, mobile calendar needs an alternative that can increase socializing time. Thus, in this study, we will collect various occupied mobile calendars and derive the scheduling strategy, in order to seek the style that represents the future and how to manage the sociality of schedule.

하늘 · 우주목의 상징성을 지닌 꽃의 현대적 변용

윤정원(Yoon, jung-Won) 동서미술문화학회 2017 미술문화연구 Vol.11 No.11

고대로부터 하늘은 초월적, 신적 존재로 여겨졌으며, 꽃과 나무는 신이 내려오는 통로이자 도구인 우주목, 신수목의 상징의미를 지니고 제천의식에서 사용되었다. 이러한 제의적 상징의미를 지닌 꽃과 나무는 한국인의 세계관과 관념에서뿐 아니라 역사와 문화, 예술 전반에 걸쳐 그 흔적을 찾을 수 있으며 주요 도상으로써 전통회화에서 자주 그려졌다. 연구자 역시 하늘의 새와 별, 그리고 꽃을 소재로 하여 작품화하고 있다. 이에 하늘과 꽃이 한국 전통문화에서 어떠한 의미와 연관을 가지고 있는지를 알아보고자 본 연구를 하게 되었다. 먼저 고대 한국인의 천(天)사상에 대해 간략히 살펴봄으로써 고대로부터 하늘은 숭배의 대상이었으며 제천의식에서 꽃과 나무는 우주목의 상징의미를 지니고 사용되었음을 알 수 있었다. 또한 한국의 신화와 무속에 나타난 꽃의 주술적 상징의미, 전통회화장르인 화조화의 기복 길상적인 성격은 종교적 하늘, 우주목의 상징의미와 연관될 수 있음을 유추해볼 수 있었다. 한편 모란은 하늘의 꽃, 서상화의 상징의미를 지니고 있으며 조선시대 궁중회화인 모란병의 수직적 구도를 비롯한 여러 조형적 특징을 보았을 때 모란병은 단순 감상용 그림이기보다는 의례를 위한 그림으로 제의적 상징의미를 지니고 있음을 알 수 있었다. 연구자는 하늘과 비상(飛翔)의 장면을 추상적으로 표현하는 것에서 시작하여 점차 시간이 지남에 따라 새와 별, 그리고 수직성이 강조된 파란 꽃 작업을 하고 있다. 앞서 행한 이론적 연구를 바탕으로 하여 연구자의 작품에 대한 분석을 한 결과 자신과 주변인들뿐 아니라 생명을 가진 모든 존재들의 안녕과 행복을 기원하는 염원 즉 공화(空花)의 의미를 담고 있는 것으로 우주목과 같이 수직적 표현을 함으로써 그 주제의식을 더욱 강조함을 알 수 있었다. 한편 주변인들의 안녕과 행복을 기원함과 동시에 인간 존재와 사회에 대한 복합적인 감정과 비판적 시각을 표현하고자 완성된 그림을 불에 태우거나 또는 마치 불에 탄 듯이 보이도록 묘사하였는데 이는 양가적이고 복층적인 불의 상징의미를 작품에 담은 것이다. 또한 연구자의 이러한 인간내면의 본성에 대한 고민과 사유는 우리에게 가치 있는 삶이란 무엇인가와 같은 질문으로 연결되었다. 그리하여 불탄 흔적에서 돋아나는 새싹과 꽃을 통해 마치 통과의례와 같은 장면을 연출하였다. 이는 불로 상징되는 상처를 그대로 표출하는 것에서 그치는 것이 아닌 삶에 대한 희망과 치유, 교감, 사랑과 같은 긍정의 메시지를 전달하고자 한 것이다. 연구자는 한국전통문화에서 하늘 우주목의 상징성을 지니는 꽃의 다양한 표현을 통해 사랑과 공감, 치유와 같은 근본적이고 보편적인 삶의 가치와 그 의미를 말하고자 하였다. 이러한 일련의 이론적 연구와 작품 분석을 통하여 한국화를 전공으로 하고 있는 연구자의 작품이 현대화단에서 어떤 위치와 맥락을 갖고 있는지 살펴보는 소중한 계기가 되었다. The sky has been deemed a transcendental, divine being since ancient times. A flower or a tree was used as a metaphor for a cosmic tree that was either a passage through which a god descends or a tool in a harvest ritual. Flowers and trees with such symbolic meaning are discovered not only in the Korean people’s worldview but also in their history, culture, and art. I take the motifs of my work from birds and stars in the sky and flowers as well. Through my theoretical studies I analyze and inquire into what meaning and association the sky and flower have in traditional Korean culture. The fact that flowers and trees were used as a metaphor for cosmic trees in a harvest ritual can be known by briefly reviewing the ancient Korean’s thought on the heavens. The symbolic meaning of flowers in ancient Korean mythology and shamanism and flower-and-bird painting’s auspicious traits can be inferred to have been associated with the symbolic meaning of religious heavens and cosmic trees. As the peony has been known as a symbol of a heavenly flower or an auspicious flower and the modeling features of the peony screen, a court painting of the Joseon Dynasty are considered, the peony screen has a symbolic meaning as a painting for a ritual, not merely as a painting for appreciation. My work started from depicting heavenly, soaring scenes abstractly and then has gradually included birds, stars and blue flowers in which verticality is underlined. As a result of analyzing my work based on theoretical studies, such thematic consciousness could be further reinforced through a vertical representation of cosmic trees that are a symbol of praying for peace and happiness of all sentient beings including myself and my surrounding people. I burned completed paintings or depicted images as if to be burned in order to pray for peace and happiness of my surrounding persons and represent my mixed emotions and critical view toward human existence and society. This was to signify fire’s ambivalent, multipronged symbolic meaning. My serious consideration and thought on the nature of humans are inextricably bound up with the question, what a worthwhile life is. A scene pertaining to a rite of passage is represented through sprouts and flowers springing from burned traces. This rendition is to convey positive messages of hope, cure, sympathy and love, moving beyond a mere representation of wounds symbolized by fire. This paper is intended to denote the underlying, universal value of life and its significance such as love, sympathy, and healing through its inquiries into diverse expressions of flowers as a symbol of a heavenly, cosmic tree in traditional Korean culture. A series of theoretical studies and work analyses serve as an invaluable opportunity to examine where my work as a Korean-style painting major is located in the context of contemporary Korean painting.