루푸스신염에서 인터루킨 - 6 의 발현

윤성철(Sung Chul Yun),윤윤수(Yun Soo Yun),빈기태(Khi Tai Bhin),신영태(Young Tai Shin) 대한내과학회 1996 대한내과학회지 Vol.51 No.5

N/A Objectives: Interleukin-6 (IL-6) is a pleiotrophic cytokines which is related to immune system activation, acute phase reactants, hematopoietic regulatory function, and cell growth as well as the first cytokine that may be directly related to pathogenesis of autoimmune disease, as systemic lupus erythematosus. The production site of IL-6 has been confined to T cell, B cell, macrophage, fibroblast, myeloma cell, myxoma cell, endothelial cell, especially mesangial cell and other glomerular cell in the kidney until now. However, recently uroepithelial cell stimulated by the bacteria and renal tublar cell damaged by immunologic injury were reported to be a another possible production site of IL-6. Therefore, Authors chose cases of lupus nephritis that was confirmed by renal biopsy. Methods: We observed the expression of IL-6 by using the immunohistochemical staining and tried to estimate the role of IL-6. The patients classified as class 4, 5 that had a strong proliferative change histologically were mainly selected as specimens for a research. The specimens were studied immunohistologically and its clinical data were compared in various ways. Results: 1) Positive IL-6 expression was observed in 16 cases (80%) of total 20 cases. IL-6 positive cases of glomerulus were accompanied with the strong IL-6 expression of renal tubules all the time. 2) The stronger IL-6 expression more than (+ +) was confirmed in 7 cases of 12 class 4 cases (87.5%). 3) The incidence of nephrotic syndrome and the case of decreased complement (C3, C4) were more prevalent in the group of positive IL-6 when compared to the group of negative IL-6. Conclusions: IL-6 has a strong association with renal tubular cells and play an important role in cellular proliferation of glomerulus as well as in disease activity of lupus nephritis.

제48차 대한내과학회 추계학술대회초록집 : 심포지움 ; 당뇨병성 신증 : 당뇨병성 신증의 임상상

윤성철 ( Sung Chul Yun ) 대한내과학회 1996 대한내과학회지 Vol.51 No.2S

제48차 대한내과학회 추계학술대회초록집 : 심포지움 ; 당뇨병성 신증 : 당뇨병성 신증의 임상상

윤성철 ( Sung Chul Yun ) 대한내과학회 1996 대한내과학회지 Vol.51 No.-

원발성 간암환자에서 관찰된 99mTc - DISIDA의 비장 섭취

윤성철(Sung Chul Yun),이예봉(Ye Bong Lee),김성진(Sung Jin Kim),박언휘(Eun Hwi Park),방인숙(In Sook Bang),김홍주(Heung Joo Kim),이원재(Won Jae Lee) 대한핵의학회 1993 핵의학 분자영상 Vol.27 No.1

We report a case of hepatorna which had a suggested splenic metastasis in a 64 year-old male patient. Hepatoma proved by abdominal CT (Cornputed Tomography) and considerable accumulation of 99mTc-DISIDA was noted in a enlarged sp]een. We suggests the splenic uptake of the radionuclide corresponded to the diffuse metasfasis of hepatorna to spleen.

낭창성신염 신조직 검색상 관찰된 아포프토시스 소견

윤성철(Sung Chul Yun),조종태(Jong Tae Cho),이재균(Jae Gyun Lee),김용진(Yong Jin Kim) 대한내과학회 1998 대한내과학회지 Vol.55 No.3

N/A Background: A few researches on apoptosis in proliferative glomerulonephritis including lupus nephritis, have been reported on the basis of animal experimental models and human renal biopsy tissues. Systemic lupus erythematosus has been reported as o disease with an apoptosis defect of lymphocyte, so that it leads to the decreased immunologic tolerance and activated autoimmunity. The lupus nephritis is frequently accompanied with a interstitial fibrosis and tubuloepithelial change, as compared to other nephitis. In addition to that, the pathologic evolution from one class to other class is also feasible and these things make us to think that the lupus nephritis is tightly linked to the apoptosis. Method: We evaluated the relationship between the apoptosis and pathologic characteristics, which was focused on class IV, in 22 patients with clinically and biopsy proven lupus nephritis by comparing with control specimens. The study on the apoptosis was observed through in situ DNA nick end-labeling method and the review on the medical history. Results: 1) An apoptosis in glomerulus was observed in only five patients (zero in Class II, 50% in Class IV) of proliferative glomerulonephritis. But 14 patients (50% in Class II, 75% in Class IV) were detected on the site of tubulo-interstitium. 2) The apoptosis detected in tubulo-interstitial area was significantly correlated with nephrotie syndrome (P<0.025), but correlations with the complement serum level, renal function and the used amount of steroid was not determined. Conclusion: The apoptosis of glomerulus in the lupus nephritis was observed in 50% of proliferative cases (especially, in class IV), but in the area of tubulointerstitium, the higher rate (more than 50%) of apoptotic expressions were observed in class II and class IV. The observation on apaptosis in renal tissue could be another prognostic factor.

비후된 심근에서의 유세포 분석을 통한 Nuclear DNA 의 관찰

김성진(Sung Jin Kim),방인숙(In Sook Bang),박언휘(Eun Hwi Park),김홍주(Heung Joo Kim),김도헌(De Heon Kim),이예봉(Ye Bong Lee),윤성철(Sung Chul Yun),한동선(Dong Sun Han),김성숙(Sung Sook Kim) 대한내과학회 1993 대한내과학회지 Vol.45 No.2

N/A Background: During the development of the cardiac hypertrophy, cytoplasmic contents of cardiac myocyte usually increase, but the changes in the nucleus of myocytes are not well understood. Therefore, we used flow cytometry to study the changes of nuclear DNA in cardiac hypertrophy from human autopsy heart tissue. Besides, we also tried to see the DNA ploidy of developing heart of fetus. Method: Nine hypertrophied human hearts ranging from 350-620 g (Group II: 350-500 g, Group III: > 500 g) and 9 control hearts (Group I: 250-350 g) which we could obtain after the legal autopsy were studied with flow cytometric analysis. And 3 fetal hearts of 20-24 gestational weeks (Group IV) were also studied. Nuclear DNA was analyzed with FACScan (Becton-Dickinson Co.) after tissue preparation using Modified Hedley and Vindelov Method and staining with Krishan staining buffer. Results: In control hearts (Group I), Flow cytometric analysis showed normal Diploidy pattern in all tissue. However, in cardiac hypertrophy, Non-Diploidy pattern was predominant (3 out of 5 specimen in Group II, and all 4 specimen in Group III) and Tetraploidy was usually observed in this abnormal ploidy pattern, but Aneuploidy was also seen in 2 cases of severe hypertrophy of Group III. Proliferative indexes increased progressively in Group I, II, and III (17.8±5.55, 24.8±7.4%, and 36.4±5.2%, respectively) (p<0.05 between Group I and II, and p<0.01 between Group I k III). Normal growing heart tissue of fetus showed normal Diploidy pattern. Conclusions: In cardiac hypertrophy, abnormal nuclear DNA ploidy pattern can be obseved. This finding might say that, during the genesis of cardiac hyper- trophy, not only cytoplasmic change but also DNA synthesis in the nucleus occurs, but G2 phase arrest happens without further mitosis.

증례 : 혈청학적으로 증명된 렙토스피라병 - 1981 년도 증례

이정상 ( Jung Sang Lee ),김성권 ( Suhng Gwon Kim ),지제근 ( Je Geun Chi ),김용훈 ( Yong Hoon Kim ),윤성철 ( Sung Chul Yun ),안규남 ( Gu Rie Ahn ) 대한내과학회 1985 대한내과학회지 Vol.28 No.5

우리나라 신고배 화상병 꽃감염 확산 가능성 및 미래 감염위험 예측을 위한 MARYBLYT 연구

김민선(Min-Sun Kim),윤성철(Sung-Chul Yun) 한국식물병리학회 2018 식물병연구 Vol.24 No.3

화상병은 우리나라에서 그동안 존재하지 않았던 금지병으로서 2015년 최초로 발병한 후 아직 박멸되지 않아, 국내 확산 연구가 필요하다. 병 삼각형 원리를 바탕으로 화상병 발병에 적합한 환경 요인을 추론하기 위해서, 최근 4년(2014-2017년) 우리나라 기상관측 자료 및 미래(2020-2100년) 기후변화 시나리오 RCP8.5 예측 기상자료를 입력하여 화상병 발병 예측프로그램인 MARYBLYT로 꽃감염을 예측하였다. 이 예측 프로그램은 신고배 개화기간을 먼저 계산한 후, 개화기간 중 매일매일의 화상병 꽃감염 위험 경보를 출력하였다. 최근 4년 동안 꽃감염 결과 화상병 발생지인 경기 안성과 천안뿐만 아니라 인근 주산지인 평택, 아산 모두 2014년과 2015년에 “Infection” 경보가 나타났다. 또한 배 주산지인 전남 나주는 2016-2017년 “Infection” 경보가 나타났다. 최근 4년간 방제 권고 수준인 “Infection” 또는 “High” 경보 발령은 우리나라 전체 중 80% 지역에서 나타나 우리나라는 화상병 발병에 적합한 환경이었다. 80년간 미래 기후변화 기상자료로 개화기간 동안 화상병 꽃감염을 예측한 결과 해마다 감염위험 지역이 급변하였다. 하루 이상 “Infection” 경보가 발령되는 지역이 전국의 50%가 넘을 정도로 광범위할 것으로 예측되는 해는 총 20년이었던 반면 “Infection” 경보가 전국의 10% 미만 지역일 것으로 예측된 해는 8년이었다. 이들 두 그룹의 환경요인 비교분석 결과, 개화기간 중 평균온도와 누적 강수량이 유의하게 달랐다. 본 연구 결과는 외래 침입균인 화상병 관리를 담당하는 정책 당국에 적절한 정보를 제공할 것이다. Since fire blight (Erwinia amylovora) firstly broke out at mid-Korea in 2015, it is necessary to investigate potential spread of the invasive pathogen. To speculate environmental factors of fireblight epidemic based on disease triangle, a fire blight predicting program, MARYBLYT, was run with the measured meteorological data in 2014-2017 and the projecting future data under RCP8.5 scenario for 2020-2100. After calculating blossom period of Singo pear from phenology, MARYBLYT was run for blossom blight during the blossom period. MARYBLYT warned “Infection” blossom blight in 2014-15 at Anseong and Cheonan as well as Pyungtak and Asan. In addition, it warned “Infection” in 2016-17 at Naju. More than 80% of Korean areas were covered “Infection” or “High”, therefore Korea was suitable for fire blight recently. Blossom blight for 2020-2100 was predicted to be highly fluctuate depending on the year. For 80 years of the future, 20 years were serious with “Infection” covered more than 50% of areas in Korea, whereas 8 years were not serious covered less than 10%. By comparisons between 50% and 10% of the year, temperature and amount of precipitation were significantly different. The results of this study are informative for policy makers to manage the alien pathogen.

자연발증 고혈압쥐의 신장과 심장에서의 아포프토시스에 대한 비교 연구

김용진(Yong Jin Kim),윤성철(Sung Chul Yun),김영권(Young Kwon Kim) 대한신장학회 2000 Kidney Research and Clinical Practice Vol.19 No.3

N/A Backgronnd: The terminal features of hypertensive target organ damage include decrease in the kidney size and increase in the heart size and wall thickness. Increased apoptosis has been known in the hypertensive nephrosclerosis and in the hypertensive heart failure. We hypothesized that apoptosis may progress by different degrees in the kidneys and heart with hypertension being sustained. To test this hypothesis we examined apoptosis in the kidneys and hearts of spontaneously hypertensive rats(SHR) of various ages. In addition, we examined histopathology of the kidneys. Methods: The kidneys and hearts of 19 SHR were excised at the age of 16 weeks(n=4), 20 weeks (n=6), and 32 weeks(n=9). Sprague-Dawley rats(SDR, n=6) were also sacrificed at the age of 16-24 weeks. Degree of apoptosis was evaluated semi-quantitati-vely by counting the number of apoptotic nuclei, stained by TUNEL method, per high power field(x400). Light microscopic and electron microscopic examination of the kidneys were performed. Results: 1) In SHR kidneys, the number of apoptotic nuclei at the age of 16 weeks was similar to that in SDR kidneys(9.3±0.5 vs. 10.2±2.2, p=NS). However, the number was significantly, p<0.05, increased at the ages of 20 weeks and 32 weeks(31.5±4.4 and 34.1±4.0, respectively) as compared with that in SHR kidneys at the age of 16 weeks and that in SDH kidneys. 2) In SHR hearts, the number of apoptotic nuclei at the ages of 16, 20, and 32 weeks(4.0±1,2, 2.0±0.7, 1.9±0.4, respectively) was neither changed nor different significantly from that in SDR hearts(0.70.5, p=N5), although the heart of SHR was hypertrophied at the age of 32 weeks. 3) Apoptosis was detected most frequently in the outer medulla of the kidneys in SHB. Histopathologic findings were the segmented sclerosis of glomeruli (1/25-50 glomeruli), edema, vacuolization and decreased villi of tubular epithelial cells. The older the age of SHR was, the more severe histopathologic changes were found. Conclusion : The sustained hypertansion caused increased apoptosis in the kidneys but no increased apoptosis in the heart of SHR during the specified ages of our study. The longer the duration of hyper- tension was, the more apoptotic cells and the more severe histopathologic changes, mainly in the tubulo-interstitial area, were found in the kidneys. The most frequent site of apoptosis was the outer medulla. It is suggested that apoptosis in the kidneys begin earlier than that in the heart in hypertensive target organ damage.

Botrytis cinerea와 Colletotrichum acutatum에 항균활성을 갖는 점액세균 Sorangium cellulosum에 대한 아종 분류 및 길항 특성 연구

구태훈(Tae-Hoon Koo),윤성철(Sung-Chul Yun) 한국식물병리학회 2018 식물병연구 Vol.24 No.3

섬유소 분해 점액세균인 Sorangium cellulosum 균주들 중 Botrytis cinerea에 길항력을 갖는다고 보고한 4균주(Antagonistic to Botrytis, AB 계열)와 Colletotrichum acutatum에 길항력을 갖는다고 보고한 2균주(Antagonistic to Colletotrichum, AC 계열)를 5가지(A-E)의 S. cellulosum 아종(subspecies)으로 분류하였다. 분류 기준 유전자인 xynB1, bglA2, groEL1 세 유전자의 유전자 유무 및 염기서열 분석을 통해 AB 계열은 아종 C, AC 계열은 아종 D로 분류하였다. 또한, 배양추출물을 고효율 액체크로마토그래피(HPLC)로 분석한 아종 분류 결과 AB 계열 4균주는 머무름시간 20-22.5분에 유사한 특징 피크가 나타난 반면, AC 계열인 2균주는 특징 피크가 없으므로 AB 계열은 아종 C, AC 계열은 아종 D임을 재확인하였다. AB 계열 4균주들의 배양여액을 사용하여 방울토마토에 전처리 후 B. cinerea를 접종한 생물검정으로 방제가와 크로마토그램 특징 피크의 상대 면적값과의 상관분석 결과, 피크 면적이 큰 균주일수록 방제가도 높은 양의 상관관계(R²=0.9652)를 확인하였다. 아종 분류 결과 AB 계열은 아종 C의 대표 생리활성 물질의 파생물인 epothilone D로 추정되어, 이를 표준시료로 HPLC 분석한 결과 epothilone D의 머무름시간은 9.9분이었던 반면, KYC 3270 특성 피크의 머무름시간은 11.581분으로 달랐다. 따라서 우리는 AB 계열이 분비하는 생리활성 물질은 epothilone의 파생물인 7-ketone epothilone D로 추정하였다. We classified the previously reported antagonistic strains of Sorangium cellulosum into 5 subspecies (A-E). Four strains were antagonistic to Botrytis cinerea (AB group) and two strains were antagonistic to Colletotrichum acutatum (AC group). According to the genetic and sequential analyses with standard genes, xynB1, bglA2, groEL1 for grouping, all strains of AB group were belonged to subspecies C and all strains of AC group were belonged to subspecies D. In addition, high pressure liquid chromatography with the culture filtrates confirmed the genetic results, because AB group had peaks with retention time at 20-22.5 minutes, whereas AC group had no peak. There was positive relationship (R²=0.9652) between the control values of infecting B. cinerea on cherry tomatoes and the main peak areas of chromatograms among the four isolates of AB group. From the subspecies results of AB group, the main peak of KYC 3270 was expected to be epothilone D. However the retention times of the standard of commercial epothilone D and the main peak of KYC 3270 culture filtrate were different as 9.9 and 11.581 min., respectively. Finally, the antagonistic metabolite of AB group was inferred as 7-ketone epothilone D.