전라북도산 브랜드 쌀의 쌀알 품질 특성과 이의 계절적 변이

이정애 ( Chong Ae Lee ),윤성중 ( Song Joong Yun ) 전북대학교 농업과학기술연구소 2011 농업생명과학연구 Vol.42 No.2

Brand rice has become the major rice product for cooking circulated and sold in markets, and consumers` reputation and royalty to any brand render competitive edge in markets. Thus, this study was conducted to evaluate quality and uniformity of brand rice from Jeonbuk province and seek ways to improve their quality. A total of twenty-two brand rice from Jeonbuk and the two national power brands were evaluated for their visual and quality traits. Seasonal uniformity of the brand quality was estimated with brand products purchased three times at an interval of three or six months from November 2008. The standard of grade classification set by the Ministry of Food, Agriculture, Forestry, and Fisheries was used for grading of brand products. Quality grades of top five brand products from Jeonbuk were similar to or better than those of the national power brand products. However, many brand products showed regular grades of quality. Number of brands with the supreme grade for head rice ratio and protein content were two and eight out of 22 brand products, respectively. Many of the brand products were graded to the lowest class for content of broken, colored, and chalky kernels. Seasonal uniformity was higher for amylose content, head rice ratio, and water content. Protein showed about 12% seasonal variability. However, traits such as head rice ratio, and ratio of chalky, damaged and colored kernels showed variability of 32 to 104 percent. These results demonstrate that majority of brand products from Jeonbuk need to reduce nitrogen fertilization to meet protein content less than 6.6%. Multiple measures are also required to reduce anti-quality kernels by right-time harvest and drying of wet rice grains, slow drying at low temperature, storage in temperature- controlled facility, and improvement of milling equipments. In addition, it is recommended to reduce numbers of brand products by merging those from the same city or county areas into a few common brand products. Local governments` branding strategies are also needed to differentiate their brand products by integrating uniqueness of their variety and production environment into brand products.

토란 유전자원의 RAPD 분석 최적조건 규명

최경구(Kyeong Gu Choi),윤성중(Song Joong Yun),유남희(Nam Hee Yoo) 전북대학교 농업과학기술연구소 1997 농업생명과학연구 Vol.28 No.-

수종의 C₄C₃작물의 엽맥과 기공 분포 특성

황종규(Jong Kyu Hwang),윤성중(Song Joong Yun) 전북대학교 농업과학기술연구소 1988 농업생명과학연구 Vol.19 No.-

나물용 콩 품종의 종실 및 콩나물 함유 Isoflavone 변이

김영진,오영진,조상균,김정곤,박명렬,윤성중,Kim, Young-Jin,Oh, Young-Jin,Cho, Sang-Kyun,Kim, Jung-Gon,Park, Myoung-Ryoul,Yun, Song-Joong 한국작물학회 2006 Korean journal of crop science Vol.51 No.suppl1

우수한 기능성을 가진 나물콩을 생산하기 위하여 isoflavone함량이 높은 품종을 선발하고, 재배적 방법에 의한 원료콩 종실의 isoflavone함량의 증진 가능성을 조사하는 한편, 콩나물의 재배 일수에 따른 콩나물 부위별 isoflavone 함량의 경시적 변화 양상을 조사한 결과를 요약하면 다음과 같다. 1. 공시한 나물콩 21품종의 종실에 함유된 daidzein과 genistein의 총 함량은 $756{\sim}1,682{\mu}g/g$으로 품종간 차이가 컸으며, 특히 익산 13호$(1,682{\mu}g/g)$ 및 남원재래$(1,500{\mu}g/g)$에서 함량이 높았다. 2. Daidzein 함량은 익산 13호$(924{\mu}g/g)$ 및 IT 160996 $(828{\mu}g/g)$에서 높았으며, genistein 함량은 익산 13호$(758{\mu}g/g)$ 및 남원재래$(756{\mu}g/g)$에서 높았다. 3. 풍산나물콩의 영양생장기에 식물생장조절제인 안티폴$(2,472{\mu}g/g)$과 후라스타$(2,052{\mu}g/g)$를 처리하였을 때 daidzein과 genistein의 총 함량이 대조군$(1,800{\mu}g/g)$에 비하여 각각 37%와 14% 증가하였다. 4. 은하콩의 콩나물 재배일수 경과에 따른 isoflavone 함량은 자엽의 경우 변화가 미미하였으나, 배축의 경우 daidzein이 치상 후 3일까지 증가하다 4일째부터 급격히 감소하는 경향을 나타냈으며, 배축의 genistein함량은 콩나물 재배기간이 경과할수록 감소하였다. 5. 풍산나물콩의 콩나물 재배일수 경과에 따른 isoflavone 함량은 은하콩과 달리 자엽의 경우에는 변화가 미미하였으나, 배축의 경우 daidzein이 치상 후 1일에 $5,154{\mu}g/g$까지 증가했으나 재배일수가 경과함에 따라 급격히 감소하는 경향을 나타냈으며, 배축의 genistein함량은 큰 변동을 보이지 않았다. 6. 콩나물재배 6일째부터 발생한 잔뿌리에는 isoflavone 함량이 $4,416{\mu}g/g{\sim}5,232{\mu}g/g$으로 매우 높았다. 7. 고기능성 나물용 콩을 육성하기 위해서는 원료콩 종실의 isoflavone함량이 높고, 콩나물이 식용으로 이용되는 시기까지 isoflavone함량이 크게 감소하지 않는 특성을 가진 품종의 육성이 필요할 것으로 생각된다. Biofuctionality of soybean seeds and soy-bean products have been fortified by the uncovering of the multifuctional beneficial effects of isoflavones. As one way to fully utilize beneficial effects of isoflavones in soybean sprout is through the enhancement of isoflavone contents in soybean seeds, genetic selection for higher isoflavone and cultivational measures to increase isoflavone content in soybean seeds were attempted. Isoflavones (daidzein, gemstein) contents in soybean seeds and soybean sprouts were determined by high performance liquid chromatography. Total isoflavone contents in soybean seeds ranged from 756 to $1,682{\mu}g/g$ and Iksan #13 $(1,682{\mu}g/g)$ showed highest content among the 21 germplasms analyzed. Onetime treatment of soybean plants with Antipol or Piaster at the $V_4$ stage yielded seeds with higher isoflavones as $2,472{\mu}g/g\;or\;2,052{\mu}g/g$, respectively, which were higher by 37% and 14% than that of seeds in the control plants, respectively. In Eunhakong, Isoflavone contents of soybean sprout changed during sprouting. Daidzein content in hypocotyl increased to maximum on the third day of cultivation and decreased there-after, whereas the content changed little in cotyledon. In sprouts of Pungsannamulkong, daidzein content in hypocotyl showed a maximum level on the first day and decreased gradually thereafter but, the content changed little in cotyledon. Total isoflavone contents in lateral roots which developed on the 6th day after sprouting ranged from 4,416 to $5,232{\mu}g/g$ DW.

쌀보리 약배양을 위한 약치상 방법별 배양효율

박태일,정선옥,김영진,김현순,서재환,박기훈,김정곤,윤성중,Park, Tae-Il,Jeoung, Sun-Ok,Kim, Young-Jin,Kim, Hyun-Soon,Seo, Jae-Hwan,Park, Ki-Hun,Kim, Jung-Gon,Yun, Song-Joong 한국식물생명공학회 2007 식물생명공학회지 Vol.34 No.3

Barley anther culture is hard working to plating picking out anther from the glume and demand long time comparing to be short available development stage for effective culture. Also, it has been treatment massive materials due to low plantlet comparing to get desirable plants intensively. Consequently, this experiment was carried out trying to be more high barley anther culture effectively in terms of save plating effort. Plating materials and culture temperature affected anther culture efficiency are among the inoculation tissues or organs such as anthers, spikelets and whole panicles, culture efficiency was higher with spikelets in two-rowed than six-rowed barley due primarily to a lower contamination, and calli were induced within 30 to 50 days. Callus induction and plant regeneration rates were higher in cultures at $25^{\circ}C$ than at $15^{\circ}C$ and $20^{\circ}C$. Days to callus induction were 25 to 50 days at $25^{\circ}C$ and 50 to 60 days at $20^{\circ}C$.

AZCA 저항성 돌연변이 세포주로부터 선발 육성만 내염성 벼 돌연변이 계통의 특성 검정

송재영,김동섭,이긍주,이인석,강권규,윤성중,강시용,Song, Jae-Young,Kim, Dong-Sub,Lee, Geung-Joo,Lee, In-Sok,Kang, Kwon-Kyoo,Yun, Song-Joong,Kang, Si-Yong 한국식물생명공학회 2007 식물생명공학회지 Vol.34 No.1

본 연구는 벼 배 배양 캘러스에 방사선 조사와 AZCA 처리를 통해 AZCA저항성 세포주를 선발 육성하고 proline 함량이 증가된 선발 계통을 중심으로 염분 스트레스에 저항성을 갖는 벼 계통을 육성하고 그 기작을 밝히고자 하였다. 먼저, 1) AZCA 저항성 후대로부터 NaCl 저항성 식물체를 선발하고, 2) 선발된 저항성 계통의 생리적 생화학적 특성을 분석하였으며, 3) 분자적 특성을 RT-PCR을 통해 조사하고 유전적 변이를 탐색하였다. AZCA저항성 $M_{3}$ 3,000 계통으로부터 얻어진 약 20,000 종자에 염분 적정 선발 농도로 밝혀진 1.5%의 염분을 처리하여 내염성 (ST) 벼 116 개체를 선발하고, $M_{4}$ 후대 세대를 양성하였다. $ST\;M_{4}$ 세대에서 2차 내염성 계통 선발을 위해서 $M_{4}$ 세대계통을 1.2% NACl 에서 대조구와 생육 조사한 결과, 대조구 식물은 생육이 약하고 성장이 지연되는 것을 볼 수 있었다. 내염성 계통(ST-13, ST-16)으로부터 유도된 캘러스에 NaCl 처리한 결과, 대조구, ST-13, ST-16의 생존율은 9%, 16%, 20%로 나타났다. 또한, 필수 아미노산 함량을 잎, 종자 및 캘러스로 나누어 분석한 결과 ST-13와 ST-16는 대조구와 비교하여, 1) 잎에서는 약 1.24, 1.3배, 2) 종자에서는 1.49, 2.43배, 3) 캘러스에서는 1.32, 1.60배 증가하는 것이 확인되었다. 내염성 계통과 대조구에서 이온함량을 비교한 결과 잎과 뿌리에서 $K^{+},\;Na^{+}$ 및 $Na^{+}/K^{+}$ 비율을 보면 대조구보다 $Na^{+}/K^{+}$ 비율이 낮아진 것이 확인되었다. 내염성과 연관된 유전자 P5CS, NHXI를 이용하여 RT-PCR 실험을 수행한 결과, 돌연변이 계통에서 이들 유전자의 발현이 증가됨을 확인할 수 있었다. 본 연구에서 선발된 계통은 내염성 육종 및 기초 연구를 위한 재료로 이용될 수 있을 것으로 사료된다. To develop rice (Oryza sativa L.) cultivars to be planted on salt-affected sites, cell lines with enhanced proline content and resistance to growth inhibition by Azetidine-2-carboxylic acid (AZCA), a proline analogue, were screened out among calli irradiated with gamma ray of 50, 70, 90, and 120 Gy. The calli had been derived from embryo culture of the cultivar Donganbyeo. Selected AZCA resistant lines that had high proline accumulation were used as sources for selection of NaCl resistant lines. To determine an optimum concentration for selection of NaCl resistant lines, Donganbyeo seeds were initially cultured on the media containing various NaCl concentrations (0 to 2.5%) for 40 days, and 1.5% NaCl concentration was determined as the optimum concentration. One hundred sixteen salt-tolerant (ST) lines were selected from bulked 20,000 seeds of the AZCA resistant $M_{3}$ seeds in the medium containing 1.5% NaCl. The putative 33 lines ($M_{4}$ generation) considered with salt-tolerance were further analyzed for salt tolerance, amino acid and ion contents, and expression patterns of the salt tolerance-related genes. Out of the 33 lines, 7 lines were confirmed to have superior salt tolerance. Based on growth comparison of the entries, the selected mutant lines exhibited greater shoot length with average 1.5 times, root length with 1.3 times, root numbers with 1.1 times, and fresh weight with 1.5 times than control. Proline contents were increased maximum 20%, 100% and 20% in the leaf, seed and callus, respectively, of the selected lines. Compared to control, amino acid contents of the mutants were 24 to 29%, 49 to 143%, 32 to 60% higher in the leaf, seed and callus, respectively. The ratio of $Na^{+}/K^{+}$ for most of the ST-lines were lower than that of control, ranging from 1.0 to 3.8 for the leaf and 11.5 to 28.5 for the root, while the control had 3.5 and 32.9 in the leaf and root, respectively. The transcription patterns for the P5CS and NHXI genes observed by RT-PCR analysis indicated that these genes were actively expressed under salt stress. The selected mutants will be useful for the development of rice cultivar resistant to salt stress.

RNA-Sequencing을 이용한 벼 품종간 수발아 차이 분석

최명구,이현석,황운하,양서영,이윤호,이충근,윤성중,정재혁,Choi, Myoung-Goo,Lee, Hyen-Seok,Hwang, Woon-Ha,Yang, Seo-Yeong,Lee, Yun-Ho,Lee, Chung-gun,Yun, Song Joong,Jeong, Jae-Hyeok 한국작물학회 2020 한국작물학회지 Vol.65 No.4

Seed dormancy is an adaptive trait in which seeds do not germinate under unfavorable environmental conditions. Low dormancy seeds are easily germinated under optimal environmental conditions, and these characteristics greatly reduce the yield and quality of crops. In the present study, we compared the pre-harvest sprouting (PHS) rate of two cultivars, Joun and Jopyeong, using the Winkler scale after heading day and temperature of the test. The PHS rate increased as the Winkler scale after heading day increased from 700℃ to 1100℃ and the temperature of the test increased. In all conditions, the PHS rate of Jopyeong was higher than that of Joun. RNA-sequencing was used to analyze the cause of the high PHS rate. We analyzed the biological metabolic processes related to the abscisic acid (ABA) metabolite pathway using the KEGG mapper with selected differentially expressed genes in PHS seeds. We found that the expression of ABA biosynthesis genes (OsNCEDs) was down-regulated and that ABA catabolic genes (OsCYP707As) was up-regulated in PHS seeds. However, the quantitative real-time PCR results showed that Joun had a higher expression of OsNCEDs than that of Jopyeong, but OsCYP707As did not yield a significant result. Joun displayed higher ABA content than that of Jopyeong not only during ripeness time but also during PHS treatment. Taken together, we provided evidence that the ABA content remaining in the seed is important to the PHS rate, which is determined by the expression level of the ABA biosynthesis gene OsNCEDs.

불가사리를 이용한 액제비료가 고추의 수량에 미치는 영향

유남희(Nam Hee Yoo),정현석(Hyun Suk Jeong),장준영(Jun Young Chang),윤성중(Song Joong Yun),김진기(Jin Key Kim),최경구(Kyeong Gu Choi),나의식(Eui Shik Rha) 전북대학교 농업과학기술연구소 2002 농업생명과학연구 Vol.33 No.-

Analysis of Plants Response to Music Signals

Keun Young Lee(이근영),Tae Won Hur(허태원),Won Chu Lee(이완주),Song Joong Yun(윤성중) 한국원예학회 2002 Horticulture, Environment, and Biotechnology Vol.43 No.1

몇가지 양분 결핍에 대한 벼 유묘의 생육반응

조중식 ( Jung Sik Jo ),박명렬 ( Myoung Ryoul Park ),윤성중 ( Song Joong Yun ) 전북대학교 농업과학기술연구소 2009 농업생명과학연구 Vol.40 No.2

건전한 어린모 육묘를 위한 유묘 초기의 무기양분 공급 필요성을 평가하기 위하여 발아 및 유묘 생육초기의 양분 결핍이 유묘 생육에 미치는 영향을 다산벼를 이용하여 조사하였다. 양분 결핍처리는 Yoshida액을 사용하여 질소, 인산, 칼륨, 칼슘, 철 등의 단일성분 결핍처리와 증류수를 이용한 모든 양분 결핍처리를 실시하였다. 양분 결핍처리를 발아시 또는 발아 5일후부터 5-10일동안 처리하여 유묘의 주요 생육형질을 조사 분석하였다. 양분 결핍에 의해 유묘의 초장, 생체중, 지상부생체중, 근장, 근생체중 등이 현저히 감소하였다. 유묘생육 저해정도는 질소 결핍구, 철분 결핍구, 모든 양분 결핍구에서 더욱 컸다. 유묘의 생육은 발아개시기보다는 유묘초기의 양분 결핍에 의해 더 감소되었다. 이상의 결과는 주요 무기 양분이 발아과정에는 미미한 영향을 주나, 유묘의 생육에는 크게 영향을 미치며, 특히 질소와 철분의 영향이 큰 것을 의미한다. 그러므로 건묘육성을 위해서는 유묘종자의 배유양분이 소진되기 이전의 유묘초기에 질소 및 철분 등 무기양분의 공급이 필요한 것으로 판단된다. Infant seedling transplanting outweighs direct seeding in stability, productivity, and product quality of rice cultivation though it requires intensive management in seedling nursery. This study was conducted to identify key mineral nutrients for the production of quality infant seedlings by analyzing the effects of mineral nutrients deficiency on early seedling growth. Deficiency of N, P, K, Ca, Fe, or all nutrients was induced by using Yoshida`s nutrient solution for up to 10 days from germination or 5 days after germination in Dasanbyeo. Seedling height and fresh weight, shoot fresh weight, root number, root length, and root fresh weight were decreased by deficiency of the nutrients, but most severely by N or Fe deficiency. This result demonstrates the relatively greater importance of N and Fe than other nutrients in early seedling growth. Seed germination was little affected by any nutrient. The results indicate that additional supply of nutrients should be considered based on the nutrients levels of nursery soil for quality seedling production.