당근 ( Daucus carota L. ) 배양세포의 DNase 활성에 미치는 Polyamines 의 영향

윤미정(Mi Chung Yoon),강영희(Young Hee Kang) 한국식물학회 1986 Journal of Plant Biology Vol.29 No.4

The present study was attempted to investigate the effects of polyamines such as putrescine, spermidine and spermine on protein content and DNase activity in vivo and in vitro in carrot embryos. It was also investigated whether polyamines could replace role of cations required for DNase activity in vitro. The results obtained are as follows. Putrescine, spermidine and spermine increased protein content, although response to spermine reached plateau at the concentration of 0.1 mM. DNase activity was inhibited by polyamines, the inhibition being concentration-dependent and the highest at the concentration of 10 mM. The inhibition of DNase activity was the most prominent with spermine. Similar inhibitory effect of polyamines which was concentration-dependent was found in DNase activity but no change was shown on time-course in vitro. Putrescine and spermidine enhanced the DNase activity at low Mg^2+ and Mn^2+ concentrations, suggesting thet the role of Mg^2+ and Mn^2+ for DNase activity could be, in part, replaced by these polyamines. These results, therefore, suggest that polyamines can modulate DNase activity through binding to DNA rather than direct effect on DNase activity.

흰쥐의 착상기간중 Estradiol이 자궁의 Phospholipase A₂ 활성도에 미치는 영향

윤미정(Mi-chung Yoon),김창미(Chang-mee Kim),최임순(Rim-Soon Choe),유경자(Kyung-za Ryu) 대한약리학회 1991 대한약리학잡지 Vol.27 No.2

본 연구에서는 흰쥐의 수정란 착상시기에 estradiol이 prostaglandins(PGs) 합성의 전구체인 arachidonic acid를 생성하는데 관여하는 phospholipase A₂(PLA₂)의 활성도를 조절하므로써 PGs의 합성을 촉진하는가를 조사하여 다음과 같은 결과를 얻었다. 자궁의 PLA₂ 활성도는 수정란이 착상하는 시기인 임신 제 5일에 증가되었으며, 비착상부위에서보다는 착상부위에서 더 높은 것으로 나타났다. Delayed implantation model에서, PLA₂ 활성도는 estradiol을 투여한 지 11시간후에 증가되었으며, dbcAMP를 투여한지 8시간후에 증가되었다. 또한 estradiol을 투여하기 2시간전에 phosphodiesterase inhibitor인 theophylline을 투여하면 estradiol만 투여한것에 비하여 PLA₂ 활성도가 증가되었다. Estradiol 또는 dbcAMP와 함께 indomethacin을 투여하면 자궁의 PGs합성은 억제되었으나 PLA₂ 활성도에는 영향을 주지않았다. 이상의 결과로 보아 흰쥐의 착상시기에 estradiol은 cAMP를 매개로하여 자궁의 PLA₂ 활성도를 촉진하므로써 PGs의 합성을 증가시키는 것으로 생각된다. The present study was performed to determine whether estradiol, via cAMP mediation, induces prostaglandin synthesis by modulating phospholipase A₂ activity which hydrolyzes phospholipids into arachidonic acids, a precursor for prostaglandin synthesis, during the implantation process in rats. Uterine phospholipase A₂ activity was elevated on day 5 of pregnancy when implantation normally occurs in rats. Moreover, phospholipase A₂ activity was higher in the implant sites than in the non-implant sites of uterus on day 6. In delayed implantation model, phospholipase A₂ activity was increased at 12 hrs after estradiol administration and at 8 hrs after dbcAMP administration. In addition, higher activity of phospholipase A₂ was induced by the treatment of estradiol plus theophylline, compared with estradiol-only treated group. The simultaneous treatment of indomethacin with estradiol or dbcAMP did not alter phospholipase A₂ activity compared with estradiol or dbcAMP-only treated group although significant suppression was observed in uterine PGE and PGE_2α concentrations. These results suggest that estradiol or cAMP stimulates uterine phospholipase A₂ activity, thereby increasing prostaglandin synthesis during the implantation process in rats.

통합 미술교육프로그램의 효과에 관한 메타분석-유아의 창의성에 미치는 효과를 중심으로-

최미정 ( Choi Mi-jung ),정정희 ( Chung Chung-hee ),윤미승 ( Yoon Mi-seung ) 한국어린이문학교육학회 2017 어린이문학교육연구 Vol.18 No.1

본 연구는 통합 미술교육프로그램이 유아의 창의성에 미치는 효과를 메타분석하기 위한 목적으로 실시되었다. 이를 위해 2006년부터 2016년까지 발표된 일반대학원 석사 및 박사학위 논문, 학술지게재 논문 총 35편을 분석대상으로 하여 통합 미술교육프로그램이 유아의 창의성에 미치는 효과를 종합적으로 분석하고, 효과크기에 영향을 미치는 조절변인들을 검토하였다. 연구결과는 다음과 같다. 첫째, 통합 미술교육프로그램이 유아의 창의성에 미치는 전체 효과크기는 1.53으로 Cohen의 효과크기 기준으로 볼 때 매우 크다. 둘째, 통합 미술교육프로그램이 유아의 창의성에 미치는 효과크기는 프로그램 유형에 따라 유의한 차이가 나타났고, 연령, 집단구성 유형, 처치횟수에 따른 유의한 차이는 나타나지 않았다. 본 연구의 결과를 바탕으로 통합 미술활동의 효과성과 교육적 당위성을 뒷받침하는 근거가 제시되었으며 향후 미술교육이 나아가야 할 방향성을 제언하였다. The purpose of this study was to analyze the effect of early childhood integrated art education programs on the creativity of young children. A meta-analysis of related domestic Master`s and Doctor`s dissertations and journal papers from 2006 to 2016 was conducted, and 38 samples of effect size from 35 dissertations were collected for this study. The findings of this study are as follows. First, the overall effect size for integrated art education programs designed for improving the creativity of young children was 1.53, which confirmed that they produced large effects. Second, there was a significant difference in the effect size. The type of program in experimental groups proved to be the moderating variables which explain the difference in the effect size for the influence of the early childhood integrated art education programs on the creativity of young children. As a result, integrated art education programs are educationally significant in improving the creativity of young children.

흰쥐 수정란 착상시기에 있어서의 호르몬 농도의 변화와 자궁내막의 구조에 관한 연구

윤미정,손성향,김창미,최임순,Yoon, Mi-Chung,Sohn, Seong-Hyang,Kim, Chang-Mee,Choe, Rim-Soon 한국현미경학회 1993 Applied microscopy Vol.23 No.1

The mechanism by which blastocysts implant to uterine endometrium has not been clearly understood. In the present study, the following question was investigated: how are hormonal levels changed and how is uterine endometrium morphologically changed? Results obtained are as follows: Concentrations of serum estradiol and progesterone were significantly increased on day 4 and 5 of pregnancy. Uterine concentrations of PGE and $PGE_{2a}$ were sharply increased on day 1 and maintained similar concentrations thereafter, reaching the maximum on day 5. Both prostaglandins (PGs) concentrations were gradually decreased thereafter. Furthermore, concentrations of PGs in implant sites were greater than those in non-implant sites. PBR (pontamine blue reaction) in uterine endometrium was positive on day 6 of pregnancy, indicating that vascular permeability was increased. Edema and changes in structure of cell components were pronounced in stroma where PBR was developed. Moreover, these were more prominent in implant sites than non-implant sites. These results suggest that uterine PGs as well as steroid hormones increase during implantation in rats and these hormones might be involved in the process of implantation by modulating vascular permeability and the fine structures of uterine endometrial cells.

cAMP Mediation in Estradiol-induced Uterine Prostaglandin Synthesis During the Delayed Implantation Process in Rats

윤미정,김창미,최임순,유경자,Yoon, Mi-Chung,Kim, Chang-Mee,Choe, Rim-Soon,Ryu, Kyung-Za The Korean Society of Pharmacology 1991 대한약리학잡지 Vol.27 No.2

본 연구에서는 흰쥐의 착상지연을 유도하여 착상기간동안 자궁조직내 prostaglandin (PG) 생합성이 어떠한 인자에 의해서 조절되는가를 관찰하여 다음과 같은 결과를 얻었다. 흰쥐의 착상지연과정동안 estradiol을 처리하면 처리후 4시간만에 자궁조직내의 cAMP의 농도가 급격하게 증가하였다. PGE와 $PGF_2{\alpha}$의 농도는 estradiol을 처리한 후 12시간이 경과하였을때 증가하였으나 $PGF_2{\alpha}$의 증가는 통계적으로 유의하지는 않았다. 또한 indomethacin을 estradiol과 동시에 처리하면 estradiol 처리로 인한 PGE와 $PGF_2{\alpha}$의 농도 증가는 나타나지 않았으나 cAMP 농도는 증가하였다. dbcAMP를 처리하면 자궁내 PGE 및 $PGF_2{\alpha}$의 농도가 증가하기 시작하여 estradiol이 투여시에 비하여 4시간 빨리 8시간후에 최고치에 도달하였으며 phosphodiesterase inhibitor인 theophylline을 전처치하면 estradiol만 투여한 것에 비하여 자궁조직내 PGE 및 $PGF_2{\alpha}$의 농도가 유의하게 증가하였다. 이상의 결과로 보아 흰쥐의 착상지연과정동안 estradiol이 자궁의 prostaglandin 합성을 증가시키며 이러한 증가는 cAMP의 증가를 매개하는 것으로 생각된다. The present study was performed to elucidate the factors which modulate uterine prostaglandin synthesis during the implantation period in rats, by employing delayed implantation model. Administration of estradiol sharply increased uterine cAMP concentration 4 hrs later during the delayed implantation process. Concentrations of uterine PGE and $PGF_2{\alpha}$ were increased at 12 hrs after the estradiol treatment although an increase in $PGF_2{\alpha}$ was not statistically significant. The concomitant treatment of indomethacin with estradiol significantly suppressed estradiol-induced PGE and $PGF_2{\alpha}$ at 12 hrs, while uterine cAMP concentration was not suppressed. The treatment of dbcAMP without estradiol gradually increased uterine PGE and $PGF_2{\alpha}$ showing the maximum 8 hrs later, suggesting that cAMP minics estradiol effect on uterine prostaglandin synthesis during the implantation process. Furthermore, the pretreatment of theophylline, phosphodiesterase inhibitor, induced significantly greater concentrations of uterine PGE and $PGF_2{\alpha}$, compared with estradiol-only treated group. These results suggest that estradiol stimulates uterine prostaglandin synthesis and this process may be mediated by an elevation of cAMP during the delayed implantation process in rats.

Modulation of Uterine Phospholipase $A_2$ Activity by Estradiol During the Delayed Implantation Process in Rats

윤미정,김창미,최임순,유경자,Yoon, Mi-Chung,Kim, Chang-Mee,Choe, Rim-Soon,Ryu, Kyung-Za The Korean Society of Pharmacology 1991 대한약리학잡지 Vol.27 No.2

본 연구에서는 흰쥐의 수정란 착상시기에 estradiol이 prostaglandins(PGs) 합성의 전구체인 arachidonic acid를 생성하는데 관여하는 phospholipase $A_2(PLA_2)$의 활성도를 조절하므로써 PGs의 합성을 촉진하는가를 조사하여 다음과 같은 결과를 얻었다. 자궁의 $PLA_2$ 활성도는 수정란이 착상하는 시기인 임신 제 5일에 증가되었으며, 비착상부위에서보다는 착상부위에서 더 높은 것으로 나타났다. Delayed implantation model에서, $PLA_2$ 활성도는 estradiol을 투여한 지 11시간후에 증가되었으며, dbcAMP를 투여한지 8시간후에 증가되었다. 또한 estradiol을 투여하기 2시간전에 phosphodiesterase inhibitor인 theophylline을 투여하면 estradiol만 투여한것에 비하여 $PLA_2$ 활성도가 증가되었다. Estradiol 또는 dbcAMP와 함께 indomethacin을 투여하면 자궁의 PGs합성은 억제되었으나 $PLA_2$ 활성도에는 영향을 주지않았다. 이상의 결과로 보아 흰쥐의 착상시기에 estradiol은 cAMP를 매개로하여 자궁의 $PLA_2$ 활성도를 촉진하므로써 PGs의 합성을 증가시키는 것으로 생각된다. The present study was performed to determine whether estradiol, via cAMP mediation, induces prostaglandin synthesis by modulating phospholipase $A_2$ activity which hydrolyzes phospholipids into arachidonic acids, a precursor for prostaglandin synthesis, during the implantation process in rats. Uterine phospholipase $A_2$ activity was elevated on day 5 of pregnancy when implantation normally occurs in rats. Moreover, phospholipase $A_2$ activity was higher in the implant sites than in the non-implant sites of uterus on day 6. In delayed implantation model, phospholipase $A_2$ activity was increased at 12 hrs after estradiol administration and at 8 hrs after dbcAMP administration. In addition, higher activity of phospholipase $A_2$ was induced by the treatment of estradiol plus theophylline, compared with estradiol-only treated group. The simultaneous treatment of indomethacin with estradiol or dbcAMP did not alter phospholipase $A_2$ activity compared with estradiol or dbcAMP-only treated group although significant suppression was observed in uterine PGE and $PGF_{2{\alpha}}$ concentrations. These results suggest that estradiol or cAMP stimulates uterine phospholipase $A_2$ activity, thereby increasing prostaglandin synthesis during the implantation process in rats.

Action Mechanism of Antiestrogens on Uterine Growth in Immature Rats

이중빈,윤미정,김창미,홍사석,유경자,Lee, Jung-Bin,Yoon, Mi-Chung,Kim, Chang-Mee,Hong, Sa-Suk,Ryu, Kyung-Za The Korean Society of Pharmacology 1990 대한약리학잡지 Vol.26 No.2

비스테로이드성 항에스트로젠제는 표적기관에서 estrogen 수용체와 상경적으로 결합하므로써 estrogen의 작용을 억제하는 것으로 알려져 있다. 비스테로이드성 항에스트로젠제는 대체로 triphenylethylene계로서 tamoxifen, clomiphene, LYl17018등이 있으며 표적기관에서 estrogen의 작용을 억제하기 때문에 estrogen과 관련된 질환을 치료하는데 이용되어 오고 있다. 본 연구에서는 생후 21-23일된 미성숙 흰쥐를 재료로 항에스트로젠제중 tamoxifen과 LY117018이 자궁세포 성장에 어떠한 영향을 미치며 어떠한 기전으로 estrogen의 작용을 길항하는지를 규명하고자, 항에스트로젠제가 estrogen작용의 중요 지포에 미치는 영향을 비교 관찰하여 다음과 같은 결과를 얻었다. Tamoxifen과 LY117018은 자궁세포에서 estrogen의 영향이 없는 경우에는 estrogen agonist로, estrogen작용하에서는 estrogen antagonist로서 작용하였다. Estrogen 작용의 여러 가지 지표에 대해 tamoxifen이 LY117018보다 agonistic effect는 더 컸으나, antagonistic effect는 LY117018이 더 큰 것으로 나타났다. Estrogen 수용체에 대한 결합능은 LY117018이 estradiol보다는 약간 낮았으나 용량에 비례하여 estrogen 수용체와 결합하였다. 그러나 tamoxifen은 estrogen 수용체에 대한 결합이 아주 낮았다. Estrogen 수용체에 대한 binding affinity는 estradiol(100%), LY117018(77%), tamoxifen(6.3%) 순으로 나타났다. 항에스트로젠제의 생체내 투여는 estrogen 존재 유무에 따라 estrogen 수용체 농도에 agonist 또는 antagonist로 작용하였다. 항에스트로젠제의 단독투여는 progesterone 수용체 생성을 증가시키나, estrogen에 의하여 유도된 progesterone 수용체 생성을 억제하였다. 이상의 결과로 보아, tamoxifen과 LY117018은 estrogen유무에 따라 흰쥐 자궁세포에서 estrogen antagonist로서 뿐만 아니라 agonist로서도 작용함을 알 수 있다. 그러나 estrogen수용체와의 결합능력이 아주 낮은 tamoxifen은, 용량에 비례하여 estrogen수용체에 결합하므로써 작용하는 LY117018과는 다른 기전으로 작용하는 것으로 생각된다. In the present study, we examined the effects of tamoxifen and LY117018 on various parameters for the estrogenic actions in order to understand the mechanism by which tamoxifen and LY117018 act on the uterine cells in 21-23 day old immature rats. Tamoxifen and LY117018 stimulated uterine weight and uterine contents of DNA, protein, and peroxidase activity in the absence of estradiol while inhibited above parameters in the presence of estradiol. Both cytosolic and nuclear progesterone receptors were increased by the treatment of tamoxifen and LY117018 as well as estradiol, but estradiol-induced increase in the progesterone receptors were reduced by the treatment of antiestrogens. These effects were enhanced by the multiple injections of antiestrogens. It seemed that tamoxifen was more agonistic than LY117018 but less antagonistic than LY117018, judged by their effects on various parameters for the estrogenic action. The affinities of estradiol, tamoxifen, and LY117018 for the estrogen receptor were $0.17{\pm}0.01nM(100%)$, $1.10{\pm}0.01nM(6.3%)$, and $0.23{\pm}0.01nM(77%)$, respectively. Furthermore, LY117018 was the competitive ligand for the estrogen receptor in dose-related manner but tamoxifen was not. Following estradiol treatment, nuclear estrogen receptor was sharply increased by 1 h, reaching the maximum by 16 h, while tamoxifen and LY117018 slightly increased nuclear estrogen receptor by 1 h and then decreased thereafter. It is therefore concluded that LY117018 is a competitive antagonist for the estrogen receptor with less estrogenic activity, compared to tamoxifen with low affinity to the estrogen receptor, and tamoxifen may act through other binding site than the estrogen receptor.

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박충화(Chung Hwa Park),김정민(Jeong Min Kim),장보국(Bo-Kook Jang),윤미정(Mi Jeong Yoon),임연진(Yeon Jin Lim) 한국원예학회 2022 한국원예학회 학술발표요지 Vol.2022 No.11

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박충화(Chung Hwa Park),김정민(Jeong Min Kim),장보국(Bo-Kook Jang),윤미정(Mi Jeong Yoon),임연진(Yeon Jin Lim) 한국원예학회 2022 한국원예학회 학술발표요지 Vol.2022 No.11

충청북도 농업용 지하수 수질평가

김충우(Chung-Woo Kim),윤미정(Mi-Joung Yun),유지윤(Ji-Yoon Yoo),김민자(Min-Ja Kim),김영호(Young Ho Kim),홍성택(Seong-Taek Hong) 한국토양비료학회 2019 한국토양비료학회 학술발표회 초록집 Vol.2019 No.8