『맥경』평기경팔맥병편과 『난경』 27, 28, 29난을 중심으로 한 기경팔맥에 관한 고찰

윤대환 ( Daehwan Youn ),신욱 ( Wook Shin ),신영일 ( Yeong Il Sheen ),이남구 ( Namgu Lee ),나창수 ( Changsu Na ) 경락경혈학회 2015 Korean Journal of Acupuncture Vol.32 No.2

Objectives : In this study we investigated characteristics of eight extra meridians based on the chapter of Pyoung Gi Kyoung Pal Maek Byoung(PGKPMB) of the Pulse Classic(Maijing; PC) and the 27, 28, and 29th question of the Classic of Difficult Issues(Nanjing; CDI). Methods : We searched out the contents of eight extra meridians in historical background, the principal and circulation of eight extra meridians, and the symptoms and pulse diagnosis of eight extra meridians on the PGKPMB of the PC and the 27, 28, and 29th question of the CDI. Results and Conclusions : About development of eight extra meridians, content which was scattered in the CDI was supplemented and developed in the CDI. Also taking over the content in the CDI, the meaning of the eight extra meridians was expanded and supplemented in the PC. In the PC, the mechanism about symptom of eight extra meridians especially has been described. Also materialization of symptom which linked to pathogenic pulse of eight extra meridians had a great impact on future generation.

효천증 침구치료 혈위 및 치법에 관한 고찰

윤대환 ( Daehwan Youn ),박상빈 ( Sangbin Park ),신영일 ( Yeong Il Sheen ),이남구 ( Namgu Lee ),나창수 ( Changsu Na ) 경락경혈학회 2015 Korean Journal of Acupuncture Vol.32 No.2

Objectives : The purpose of this study is to investigate acupoints that appear in ancient records and use with regard to treatment to the wheezing and dyspnea. Methods : We investigated 10 ancient records from Song Dynasty to the Qing Dynasty that had medical references to wheezing and dyspnea. Results and Conclusions : Out of acupoints that found out to be used for treatment of wheezing and dyspnea in this study, Conception and Governor channels account for 38.9%, the three yang meridians of the foot account for 22.2% and the three tin meridians of the Hand account for 13.9%. In 10 ancient records, CV22(Tian tu) appears 5 times, CV12(Zhongwan) and ST36(Zusanli) appear 4times. A total number of acupoints that appear in the ancient records were 35. In compendium of medicine, the number of acupoints was 21. In cmpendium of acupuncture and moxibustion, the number of acupoints was 10. In the treatment of wheezing and dyspnea, not only acupuncture but also moxibustion has a great importance.

환도·양릉천 레이저 및 전침이 관절염에 미치는 영향

김미래,이유미,최동희,윤대환,나창수,Kim, Mirae,Lee, Yumi,Choi, Donghee,Youn, Daehwan,Na, Changsu 경락경혈학회 2019 대한침구의학회지 Vol.36 No.4

Objectives : This study aimed to investigate the effects of electroacupuncture (EA), the laser acupuncture (LA) and the combination therapy (LA+EA) in a collagenase-induced osteoarthritis rat model. Methods : Osteoarthritis rat model was induced by injection of collagenase into left lower articular cavity (50 μl to knee and 10 μl to ankle). In order to assess the anti-osteoarthritic effects of EA, the 650 nm LA and 650 nm LA+EA, the histopathological findings and plantar withdrawal responses were analyzed. Results : All of the treatment methods used in this study were effective in reducing pain. All treatment groups were effective in decreasing inflammatory cytokines of TNF-a and IL-6; the 650 nm LA and 650 nm LA+EA groups significantly reduced IL-1β. Conclusions : 650 nm LA and EA inhibit the production of collagenase-induced inflammatory mediators of osteoarthritis.

태충 침자가 간 미토콘드리아 내 Complex IV에 미치는 영향

최동희,이유미,김미래,박정혜,김혜란,나창수,윤대환,Choi, Donghee,Lee, Yumi,Kim, Mirae,Park, Jeonghye,Kim, Hyeran,Na, Changsu,Youn, Daehwan 경락경혈학회 2019 대한침구의학회지 Vol.36 No.4

Objectives : The liver is rich in mitochondria and it plays a key role in whole-body energy homeostasis. Mitochondria is double membrane-bound organelle that supplies energy for intracellular metabolism including Krebs cycle and beta-oxidation. Acupuncture is known to stimulate and regulate the flow of energy. To explore the effect of acupuncture on the mitochondrial respiratory chain activity in the rats' livers, the activity of mitochondrial respiratory chain complexes I to IV was observed. Methods : The rats were divided into 4 groups; Normal 1 (no acupuncture treatment and anesthesia for 5 min), Normal 2 (no acupuncture treatment and anesthesia for 10 min), MA1 (acupuncture treatment at bilateral LR3 under anesthesia for 5 min), and MA2 (acupuncture treatment at bilateral LR3 under anesthesia for 10 min). All rats were sacrificed and the livers were examined for respiratory chain change. Results : There was no difference in ubiquinon oxidoreductase, succinate dehydrogenase, and ubiquinol cytochrome C oxidoreductase after acupuncture at LR3. Acupuncture at LR3 for 10 min increased the activity of cytochrome C oxidase compared with no acupuncture groups. Conclusions : Acupuncture at LR3 mediated mitochondrial respiratory chain activity via the cytochrome C oxidase signaling pathway in the livers of rats.

족삼음경의 락혈에 시술된 침 자극에 의한 NO, NOS, NE 발현 연구

이유미 ( Yumi Lee ),신욱 ( Wook Shin ),최동희 ( Donghee Choi ),김미래 ( Mirae Kim ),나창수 ( Changsu Na ),윤대환 ( Daehwan Youn ) 대한경락경혈학회 2017 Korean Journal of Acupuncture Vol.34 No.1

The acupuncture about acupoint affects the production of NO, NOS, and NE.Local action of acupuncture is important for acupuncture treatment. To prove this, the revelation degree of NO, NOS, and NE was observed by stimulating the acupuncture at the connecting point of SP4, KI4, and LR5 in the depths of Superficial layer, Middle layer and Deep layer. Methods : Needles were inserted into rats, on each right and left sides of the connecting point, SP4, KI4 and LR5 acupoints which are the stream points of the foot meridian. After insertion, needles were retained for three minutes. After the retention, rat was sacrificed via cardiac puncture, and tissues of each SP4, KI4 and LR5 point near meridian vessel was extracted to examine the changes in the expression of NO, NOS and NE. Results : In terms of the effect in NO production, there was significant increase in the Superficial layer, Middle layer and Deep layer at KI4. In terms of the effect in NE production, there was significant decrease in the Superficial layer at SP4 and increase in the Superficial layer, Middle layer and Deep layer at LR5. In terms of the effect in nNOS production, there was significant increase in the Superficial layer, Middle layer and Deep layer at SP4 also in the Superficial layer at KI4. In terms of the effect in eNOS production, there was a significant increase in the Superficial layer, Middle layer and Deep layer at SP4, KI4 and LR5. In terms of the effect in iNOS production, there was significant increase in the Superficial layer, Middle layer and Deep layer at SP4, KI4 and LR5. Conclusions : The effect of acupuncture applied at the connecting point of six meridians of the foot on the activities of NO, NOS and NE could be observed, and it can be induced from the effect of needle stimulation on disrupted local and systemic nervous responses.

수소음심경 오수혈 혈위별 레이저 침자가 고혈압 흰쥐의 혈압에 미치는 영향

신욱 ( Wook Shin ),이유미 ( Yumi Lee ),김왕인 ( Wangin Kim ),최동희 ( Donghee Choi ),김미래 ( Mirae Kim ),윤대환 ( Daehwan Youn ),나창수 ( Changsu Na ) 대한경락경혈학회 2017 Korean Journal of Acupuncture Vol.34 No.1

The purpose of this study is to compare the effects of laser acupuncture to the 532 nm on the five transport points with the heart meridian for treatment to hypertensive disease in rats with L-NAME induced hypertensive. Methods : Hypertensive was induced by L-NAME for 3 weeks. The laser acupuncture therapy on the five transport points of heart meridian(Laser Well Point-HT9, Laser Brook Point-HT8. Laser Stream Point-HT7, Laser River Point-HT4 and Laser Sea Point-HT3) was treated twice a week for 5 times. The hypertensive was measured using a cardiac hypertrophy, atherogenic index, TG/HDL-cholesterol ratio, TCHO, HDLC, TG, AST, ALT, antioxidative effectiveness and glutathione peroxidase quality of hypertensive rats induced by L-NAME. Results : Blood pressure were decreased significantly after the laser acupuncture of Well, Brook and Sea Point groups. Cardiac hypertrophy were decreased at the laser acupuncture of Brook and Stream Point groups. Athrogenic index was decreased at the laser acupuncture of Well, Stream, River and Sea Point groups. TG/HDL-cholesterol ratio was decreased at the laser acupuncture of all groups. Total cholesterol was decreased significantly at the laser acupuncture of Well Point group. High density lipoprotein cholesterol and total cholesterol were decreased significantly at the laser acupuncture of Well, Stream and River Point groups. Triglyceride was decreased significantly at the laser acupuncture of Stream Point group. Cu/Zn-Superoxide Dismutase (Cu/Zn-SOD) and glutathione peroxidase(GPX) were increased significantly at the laser acupuncture of Well Point group. Conclusions : The laser acupuncture treatment in five transport points of the Heart Meridian was effective for lowering blood pressure, cardiac hypertrophy, Atherogenic index and HTR, and for enhancing antioxidant activity.

심경, 신경의 오수혈 침자가 Mitochondrial Respiratory Chain Complexes에 미치는 영향

최동희 ( Donghee Choi ),이유미 ( Yumi Lee ),김미래 ( Mirae Kim ),박정혜 ( Jeonghye Park ),김혜란 ( Hyeran Kim ),나창수 ( Changsu Na ),윤대환 ( Daehwan Youn ) 경락경혈학회 2020 Korean Journal of Acupuncture Vol.37 No.1

Objectives : Mitochondria are typically known as intracellular double membrane-bound structures that supply energy for intracellular metabolism including Krebs cycle and beta-oxidation. Also, acupuncture has been known to stimulate the flow of energy. To explore the effect of acupuncture on the mitochondrial respiratory chain activities in rat’s heart and kidneys, the activities of mitochondrial respiratory chain complexes I to IV were observed. Methods : The rats were divided into 11 groups; Normal (no acupuncture treatment and under anesthesia for 10 min), heart meridian five-transport-points (acupuncture treatment at HT9, HT8, HT7, HT4 and HT3 under anesthesia for 10 min), and kidney meridian five-transport-points (acupuncture treatment at KI1, KI2, KI3, KI7 and KI10 under anesthesia for 10 min). All rats were sacrificed and the heart and kidneys were examined for the changes of respiratory chain activities. Results : Acupuncture at HT7 increased the activity of succinate dehydrogenase; acupuncture at KI2 increased the activity of ubiquinol cytochrome C oxidoreductase; and acupuncture at HT9, HT8, HT3 and KI1 increased activities of cytochrome C oxidase. Conclusions : Acupuncture assists mitochondrial repiratory chain activity via the Cytochrome C oxidase signaling pathway in heart and kidney of rats.

수삼음경의 락혈 침자가 백서의 혈위 조직내 nNOS. NO와 조직 및 혈장 Norepinephrine의 변화에 미치는 영향

신욱 ( Wook Shin ),이유미 ( Yumi Lee ),이경인 ( Kyoungin Lee ),최동희 ( Donghee Choi ),김미래 ( Mirae Kim ),나창수 ( Changsu Na ),김선민 ( Sunmin Kim ),표병식 ( Byoungsik Pyo ),윤대환 ( Daehwan Youn ) 대한경락경혈학회 2016 Korean Journal of Acupuncture Vol.33 No.2

Objectives : A previous study demonstrated that the connecting points of three yang meridians attenuated changes of nNOS, and Norepinephrine(NE) in rats. The current study investigated the changes in nNOS, NO and NE upon the needle insertion at varying depths at the connecting point of three yin meridians of the hand. Methods : Needles were inserted into rats, on both left and right sides of the connecting point, including the LU7, HT5 and PC6 acupoints which are three yin meridians of the hand. After insertion, needles were retained for five minutes. Each acupuncture groups were treated acupuncture at each acupoint and at the depths of superficial, middle and deep layer. After the retention, blood was drawn via cardiac puncture, and tissues of each point near meridian vessel was extracted to examine the changes in the expression of nNOS, NO and NE. Results : Compared with the superficial layer group, nNOS production significantly decreased in the middle and deep layer at LU7 acupoint group and in the deep layer at HT5, PC6 acupoint group. The tissue NE decreased in the deep layer on PC6 acupoint and the plasma NE increased at the middle layer at LU7 acupoint group but decreased at the deep layer on at LU7 acupoint group. Conclusions : Acupuncture at connecting points of three yin meridians of the hand can regulate the activities of nNOS, and NE.