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미꾸라지로부터 $\beta$-Mannosidase를 생산하는 Chryseobacterium meningosepticum의 분리
김현숙,윤기홍,Kim, Hyeon-Suk,Yun, Gi-Hong 한국미생물·생명공학회 2004 한국미생물·생명공학회지 Vol.32 No.4
A bacterium producing the $\beta$-mannosidase was isolated from intestine of fresh fish. The isolate YB-29 has been identified as Chryseomeningosepticum on the basis on its 16S rRNA sequence, morphology and biochemical proper The $\beta$-mannosdiase activity was detected in both the culture filtrate and the cell extract of C. meningosepticum YB-29. The $\beta$-mannosidase of culture filtrate showed the maximum activity for hydrolysis of para-nitrophenyl-$\beta$-D-mannopyranoside at pH 5.0-6.0 and 55-$60^{\circ}C$. The enzyme was able to hydrolyze the oligomeric substrates such as mannobiose and mannotriose with higher activity for mannotriose than mannobiose.