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가축에서 테트라사이클린 내성 장구균 조사 및 분자생물학적 특성규명
김철민 ( Chul Min Kim ),강수진 ( Su Jin Kang ),이병종 ( Beyong Jong Lee ),이성재 ( Sung Jae Lee ),육대수 ( Dae Su Yuk ) 한국가축위생학회 2010 韓國家畜衛生學會誌 Vol.33 No.2
BIn the present study, Enterococcus isolates originating from livestock were studied for the phenotypic and genotypic assessment of tetracycline resistance. A total of 74 isolates encompassing the species Enterococcus faecalis (n=12) and E. faecium (n=62) displayed phenotypic resistance to tetracycline. Tetracycline resistance gene [tet (M), 1,886bp] were sequenced by dye terminator cycle sequencing method and compared with tet (M) sequences available from the GenBank database. Sequencing analysis of PCR amplicons showed high homology to the reference strains ranging 97.2~100%. The tet (M) genes were divided into three major subgroups according to phylogenetic analysis. The genetic information obtained from this study could be useful for the molecular study of enterococci.