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유현준,배창득,김현철,윤영진,김명준,신현정,Yoo, Hyun-Jun,Bae, Chang-Deuck,Kim, Hyun-Chul,Yoon, Young-Jin,Kim, Myung-Jun,Shin, Hyun-Jung 한국진공학회 2010 Applied Science and Convergence Technology Vol.19 No.2
One-dimensional nanostructures have been researched widely because of its unique physical properties such as optical, electrical, mechanical, and chemical properties in comparison with bulk structures. Especially nanotubular structures are able to provide larger surface area, capability to load purposeful materials, and unique mechanical modulus. We reviewed the oxide nanotube technology with focusing on the method of template-directed fabrication. We can easily control of physical dimensions of nanotubes by control of nanotemplate and fabrication condition. and template-directed fabrication is ideal tool to fabricate the amount of monodisperse nanotubes. They have potentials for application in solar cell, drug-delivery, Li-ion batteries and photocatalyst. We discussed these potential applications and research trends. 일차원 나노튜브는 구조는 높은 비표면적, 내부의 빈 공간 및 특유의 물리적 특징을 제공한다. 1차원 산화물 나노튜브 구조물의 합성 방법에 따라 나누어 정리하여 논의하였다. 나노 기판을 이용한 나노튜브 합성은 고른 물리적 구조를 가지는 나노튜브를 대량으로 합성하는데 있어서 이상적인 방법으로서 기판의 형태를 상대적으로 용의하게 조절함으로써 1차원 나노튜브 구조물의 특성을 극대화하였다. 극대화된 특성을 이용한 여러 응용 분야에 대한 연구를 정리하여 제시하였다.
근접장 마이크로파 현미경을 이용한 Copper(II)-phthalocyanine의 Phase Transition 연구
박미화,유현준,윤순일,임은주,이기진,차덕준,이용산,Park, Mie-Hwa,Yoo, Hyun-Jun,Yun, Soon-Il,Lim, Eun-Ju,Lee, Kie-Jin,Cha, Deok-Joon,Lee, Young-San 한국전기전자재료학회 2004 전기전자재료학회논문지 Vol.17 No.6
We report the changes of the microwave reflection coefficients S$_{11}$ of copper(II)-phthalocyanine (CuPc) thin films by using a near-field microwave microscope(NSMM) in order to understand the phase transition of CuPc. For a NSMM system, a high-quality microstrip resonator coupled with a dielectric resonator was used. CuPc thin films were prepared on the pre-heated glass substrates using a thermal evaporation method. The reflection coefficients S$_{11}$ of CuPc thin films were changed by the dependence on the substrate pre-heating temperatures. By comparing reflection coefficient S$_{11}$ and crystal structures, we found the phase transition of CuPc thin films from $\alpha$-phase to $\beta$-phase at the substrate heating temperature 200 $^{\circ}C$..
금속/copper(Ⅱ)-phthalocyanine 계면에서의 Space Charge 연구
박미화,유현준,유형근,나승욱,김송희,이기진,Park, Mie-Hwa,Yoo, Hyun-Jun,Yoo, HyungKun,Na, Seunguk,Kim, Sonshui,Lee, Kie-Jin 한국전기전자재료학회 2005 전기전자재료학회논문지 Vol.18 No.4
We report the space charge and the surface potential of the interface between metal and copper(Ⅱ)-phthalocyanine(CuPc) thin films by measuring the microwave reflection coefficients S/sub 11/ of thin films using a near-field scanning microwave microscope(NSMM). CuPc thin films were prepared on Au and Al thin films using a thermal evaporation method. Two kinds of CuPc thin films were prepared by different substrate heating conditions; one was deposited on preheated substrate at 150。C and the other was annealed after deposition. The microwave reflection coefficients S/sub 11/ of CuPc thin films were changed by the dependence on grain alignment due to heat treatment conditions and depended on thickness of CuPc thin films. Electrical conductivity of interface between metal and organic CuPc was changed by the space charge of the interface. By comparing reflection coefficient S/sub 11/ we observed the electrical conductivity changes of CuPc thin films by the changes of surface potential and space charge at the interface.
Real-time PCR과 Colony forming unit법을 이용한 타액 내 2종의 구강미생물 총량분석
유수민 ( Su-min Yoo ),정성국 ( Seong-kug Jeong ),유현준 ( Hyun-jun Yoo ),장종화 ( Jong-hwa Jang ) 한국치위생학회(구 한국치위생교육학회) 2017 한국치위생학회지 Vol.17 No.1
Objectives: The purpose of this study was to compare colony forming unit (CFU) method and multiplex real-time polymerase chain reaction (MRT-PCR) method for accurate quantitative analysis of bacteria. Methods: We compared the CFU method and the MRT-PCR method, which are still used in Korea, for Prevotella intermedius (P. intermedius), a periodontal disease pathogen selected by MRT-PCR, and Streptococcus mutans (S. mutans), a dental caries causative organism. The subjects of this study were 30 patients who visited the C dental hospital. Results: Total microorganisms in MRT-PCR method were significantly higher in both types of bacteria (p<0.05), since DNA of dead bacteria was also analyzed. This was because the periodontal dise(-) anaerobes, and even dead bacteria contain large amounts of toxic substances called LPS in the extracellular membrane, and fimbriae and pili, which are motility structures, still remain as a strong toxic substance in periodontal tissue. Conclusions: Therefore, in terms of the total amount of bacteria found, the MRT-PCR method will be a useful technique for searching all the bacteria in the oral cavity including live bacteria, as well as sterilization.
치아우식증 유발 세균에 대한 CPC 함유 구강청정제의 항균효과
조원호 ( Won-ho Cho ),조자원 ( Ja-won Cho ),유현준 ( Hyun-jun Yoo ),신경훈 ( Kyong-hoon Shin ),신기해 ( Gi-hae Shin ),전열매 ( Yeol-mae Jeon ),이종천 ( Jong-cheon Lee ) 대한예방치과·구강보건학회 2021 大韓口腔保健學會誌 Vol.45 No.2
Objectives: The purpose of this study was to evaluate and report the antibacterial efficacy in relation to oral disease-causing bacteria using a mouthwash containing 0.05% CPC in an in vitro test. Methods: The sterilization test and susceptibility assay of mouthwash containing 0.05% CPC were investigated against Streptococcus mutans, Streptococcus sobrinus, and Lactobacillus acidophilus; Streptococcus sanguinis as oral bacteria related to dental caries; Enterococcus faecalis as apical periodontitis-related bacteria; and Actinomyces israelii, Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, Prevotella intermedia, Prevotella nigrescence, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, and Filifactor alocis as periodontal disease-related bacteria. Results: In the sterilization test, most of the bacteria had more than 99.99% sterilizing power for all samples but compared to other bacteria, the sterilizing power of these samples was not successful for L. acidophilus and E. faecalis bacteria. When comparing the sterilization power between the samples, sample 3 (0.05% CPC+20% ethanol) was the strongest. Conclusions: In the antimicrobial activity test, sample 3 inhibited growth at the lowest concentration overall.
이채영 ( Chae-young Rhee ),조자원 ( Ja-won Cho ),유현준 ( Hyun-jun Yoo ),닛타삭폼마봉싸 ( Nitthasack Phommavongsa ),안용수 ( Yong-su Ahn ),오형석 ( Hyung-suk Oh ) 대한예방치과·구강보건학회 2021 大韓口腔保健學會誌 Vol.45 No.2
Objectives: The aim of this study was to determine the dental caries experience of children in Laos. Methods: Oral examinations were performed on a total of 1,540 students in 513 primary school students, 537 middle school students, and 490 high school students and the results analyzed. Results: The dft index (decayed-filled primary teeth index) of 6-year-old primary school children was 6.04. The DMFT index (decayed-filled-missing permanent teeth index) was 1.59 in 12-year-old middle school children and 2.04 in 15-year-old middle school children. Conclusions: Caries experience in most of the age groups was on the high side. It is considered that in Laos, a treatment project to stop the progression of caries is necessary in parallel with a prevention project to lower the caries fatality rate.
김민정 ( Min-jeong Kim ),이명구 ( Myung-gu Lee ),이준행 ( Joon-haeng Lee ),전열매 ( Yeol-mae Jeon ),유현준 ( Hyun-jun Yoo ) 대한예방치과·구강보건학회 2021 大韓口腔保健學會誌 Vol.45 No.4
Objectives: This study aimed to evaluate the antibacterial activity of phytoncide on oral biofilm. Methods: Antibacterial activity of phytoncide was examined on the bacteria Streptococcus mutans, Aggregatibacter actinomycetemcomitans, Enterococcus faecalis, Porphyromonas gingivalis, and oral biofilm. After mixing the phytoncide, S. mutans, A. actinomycetemcomitans, E. faecalis, and P. gingivalis with a culture alone turbid and mixed, and they were then incubated at 37°C under anaerobic conditions and cultured. Following incubation, a microplate reader was used to measure the absorbance and observe the amount of bacteria. In addition, non-polarized saliva containing bacteria was grown for 72 h under anaerobic conditions for the determination of biofilm biomass and bacterial numbers. Various concentrations of phytoncide were added to the saliva biofilm. Statistical significance tests were conducted using the Mann-Whitney test and SPSS 24.0. Results: S. mutans, A. actinomycetemcomitans, E. faecalis, P. gingivalis, and antibacterial effects were shown as phytoncide concentrations increased in oral biofilm. Observing the growth of bacteria using phase difference microscopes showed that the number of bacteria decreases as the phytoncide concentration increased. Also, the formation of biofilm in the saliva decreased compared to the control group. Conclusions: Based on the experimental results of phytoncide on oral biofilms causing oral cavity formation in the saliva decreased compared to that in the control group. Phytoncide showed antibiosis against oral biofilms when it remained inside the mouth for above certain concentrations. Accordingly, using phytoncide as a clinical method for preventing oral disease is deemed to be effective.
니코틴 농도에 따른 치은 섬유아세포의 증식 억제에 대한 연구
한강욱 ( Kang-uk Han ),이천희 ( Cheon-hee Lee ),이준행 ( Joon-haeng Lee ),전열매 ( Yeol-mae Jeon ),유현준 ( Hyun-jun Yoo ) 대한예방치과·구강보건학회 2020 大韓口腔保健學會誌 Vol.44 No.4
Objectives: To investigate the effect of nicotine on the healing of an oral cavity wound, high and low concentrations of nicotine were administered on human gingival fibroblasts. Methods: Nicotine at concentrations of 0.1, 1, 5, and 10 mM were administered to gingival fibroblasts to evaluate the survival capability of the cells. Nicotine at 0.1 mM, a nonapoptotic concentration, was administered to evaluate apoptosis using Annexin V-FITC/Propidium Iodide cell staining. Nicotine at 1, 10, and 100 mM were administered to measure the expression of inflammatory cytokines, which was measured by RT-PCR and ELISA. FGF was treated with an additional 1, 10, or 100 mM of nicotine to evaluate cell proliferation and wound healing. Results: As the concentration of nicotine increased (0.1, 1, 5, and 10 mM), the survival capability of the cells reduced. When cells were exposed to low nicotine concentration (0.1 mM) for 24 h, apoptosis occurred. Moreover, if the cell was exposed for 48 h, cell apoptosis occurred with necrosis. As the concentration of nicotine increased (1, 10, and 100 mM), more inflammatory cytokines were expressed. When EC LPS and TF LPS were combined with a low concentration of nicotine (1 and 10 mM), the expression of inflammatory cytokines was suppressed. The FGF level decreased as the nicotine concentration increased (1, 10, and 100 mM). Conclusions: Nicotine interferes with the wound healing process of gingival fibroblasts. To maintain the wound healing process after a surgery or dental procedure, cessation of smoking is recommended.