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젖소 목장에서 분리된 황색포도상구균의 아형 분포와 특성
유종현,박희명,오태호,손대호,한홍율,Yoo, Jong-hyun,Park, Hee-myung,Oh, Tae-ho,Sohn, Dae-ho,Han, Hong-ryul 대한수의학회 1999 大韓獸醫學會誌 Vol.39 No.5
Staphylococcus aureus is one of most prevalent intramammary pathogens and have characteristics which are not easily eradicated. Recently, to understand the sources and transmission of S aureus, many studies have focused on the subtyping of field isolate. This study was preformed to investigate the distribution pattern and characteristics of the isolates using phenotyping and genotyping. Samples were collected from milk of each udder, cow bodies (perianal region, vagina, tail, udder skin, sole) and environment (floor, liner, milker's hands, water, towel, insect) from 6 herds located in Kyung-gi province. Forty five strains of S aureus were isolated from 3 dairy herds (A, B, C) and were typed by hemolytic pattern, antibiotic resistant pattern, enterotoxin typing and PCR-based DNA fingerprinting. Slime productivity was also compared by each subtype to examine potential infectiousness. Of 45 strains, 41 were isolated from milk samples and 4 were isolated from liners. No strains isolated in the bodies and environment. Forty five strains isolated were classified as 18 subtypes by phenotyping and genotyping. There was common subtype between A and B herd, but the subtype of C herd showed different pattern. Among predominant subtypes, 60% of S aureus strain isolated from A and B herd showed subtype I and 50% of S aureus strain isolated from C herd belonged to subtype VI and X II. Neither somatic cell count (SCC) nor slime production was significantly different between predominant and minor subtypes. In summary, the study revealed that liners play more important roles in the mode of transmission than environmental sources. Several subtypes can be found in a herd, only a few subtype, however, was largely associated with the majority of infection.
초미세 유체 제어 시스템 구현을 위한 마이크로 펌프와 밸브의 집적
유종철(Jong-Chul Yoo),허현정(Hyun-Jung Her),최영진(Y.J. Choi),강치중(C. J. Kang),김한수(Han-Soo Kim),이경일(Kyoung-Il Lee),신진국(Jin Koog Shin),김용상(Yong-Sang Kim) 대한전기학회 2006 대한전기학회 학술대회 논문집 Vol.2006 No.7
Micro ElectroMechanical Systems (MEMS) 기술을 이용한 초미세 유체 제어 시스템 (마이크로 펌프, 마이크로 밸브, 마이크로 채널, 마이크로 믹서 등)은 화학, 생명분야의 DNA 분석, 항원-항체 분석, 질병의 진단 등에 사용되는 lab-on-a-chip, micro total analysis system (μ-TAS) 등에서 화학 및 바이오 유체를 제어하는 분석 시스템의 일부분으로서 사용 되며 필수적으로 요구된다. 본 논문에서는 이러한 microchip을 구현하기 위해 초미세 유체 제어 소자인 마이크로 펌프와 밸브를 같은 기판 위에 polydimethylsiloxane (PDMS)와 indium tin oxide (ITO-)-Glass를 사용하여 통일한 구조로 집적 하였다. 마이크로 펌프의 pumping rate은 인가 직류 펄스 전력의 주파수와 duty 비를 변화시켜 최적화 하였다. 직류 펄스 전력 때 500 ㎽를 인가하였을 때 주파수 2 ㎐, duty 비 7%에서 약 1.05 μl/min의 최대 유량이 측정되었다. 마이크로 밸브는 ITO 히터에 전력을 인가함으로서 유량의 on/off 제어가 잘 됨을 확인할 수 있었고 유체를 closing하기 위해 필요한 전력은 약 300 ㎽이다.
유종석,최진호,한규승,한양수,이창교,이낭호,Yoo Jong-Seok,Choy Jin-Ho,Han Kyoo-Seung,Han Yang-Su,Lee Chang-Kyo,Lee Nang-Ho 대한화학회 1991 대한화학회지 Vol.35 No.4
천연규산 알루미늄광인 힐로이사이트 광물로부터 고순도 ${\alpha}-Al_2O_3$를 합성하기 위해 황산용액을 사용한 습식합성법을 이용하였다. 추출된 황산 알루미늄 용액내에 존재하는 각종 불순물을 제거하기 위해 각 금속이온의 수산화물과 탄산화물의 형성을 고려, pH에 따른 용해도곡선을 도시하였다. 이를 기초로 ${\alpha}-Al_2O_3$의 전구물질인 순수한 암모니움 명반은 pH = 1.5∼2.5에서, 수산화 알루미늄은 pH = 6∼8에서 각각 합성하였으며, 이로부터 얻어진 ${\alpha}-Al_2O_3$의 순도는99.7${\%}$와 99.0${\%}$였다. 여기서 불순물에 대한 정제효과는 암모니움 명반으로부터 합성된 경우(Na = 0.047${\%}$, Si = 0.092${\%}$)가 수산화 알루미늄으로부터 합성된 경우Na = 0.29${\%}$, Si = 0.12${\%}$)보다 매우 높은 결과를 보였다. High-purity alumina powder was prepared by extracting the natural alumino-silicate mineral (halloysite) in H$_2$SO$_4$ solution. For the selective precipitation of alum and aluminum hydroxide, the solubility diagram was prior calculated by also considering the formation of hydroxides and carbonates for all the metal ions in an aqueous solution, which allow us to control the contamination of impurities envolved in the natural minerals. Ammonium aluminum sulfate (alum) and alumium hydroxide could be successfully prepared at pH = 1.5∼2.5 and pH = 6∼8, respectively according to our solubility diagrams. The purity of alum-and hydroxide-derived ${\alpha}-Al_2O_3$ was determined to be 99.7${\%}$ and 99.0${\%}$, respectively, which indicates the former route would be more desirable for the large scale application. It is also worthy to note that the impurities like Na and Si were strongly reduced in the former (Na = 0.05${\%}$, Si = 0.09${\%}$) compared to the latter (Na = 0.29${\%}$, Si = 0.12${\%}$).
재활용 잔골재 콘크리트의 물리적 특성에 관한 섬유보강 효과
김호동,유종성,김무한,김규용,김영덕,Kim, Ho-Dong,Yoo, Jong-Seong,Kim, Moo-Han,Kim, Gyu-Yong,Kim, Young-Duck 한국섬유공학회 2008 한국섬유공학회지 Vol.45 No.1
In order to improve the physical properties of recycled fine aggregate concrete, fiber reinforced concrete composites were prepared with synthetic fibers such as polyvinylalcohol(PVA), nylon, polypropylene(PP), kevlar, and carbon fiber and were investigated for their effects on the physical properties of these concrete composites. The compressive strength of these fiber reinforced concretes using hydrophilic fibers such as PVA and nylon was explicitly improved compared to that of recycled fine aggregate concrete without fiber. On the other hand, the flexural strength did not indicate the substantial changes even though high performance fiber was used. In addition to the strength reinforcing effect, the porosity of recycled fine aggregate concrete evidently decreased with fiber reinforcement, thus, making the concrete denser.
직장암에서 복강경 수술과 개복 수술 후 단기간의 종양학적 결과에 대한 비교
최낙준(Nak Jun Choi),유종한(Jong Han Yoo),이홍태(Hong Tae Lee),신재호(Jae Ho Shin),박하경(Ha Kyoung Park),안민성(Min Sung An),하태권(Tae Kwun Ha),김광희(Kwang Hee Kim),배기범(Ki Beom Bae),김태현(Tae Hyun Kim),최창수(Chang Soo Choi),오 대한종양외과학회 2013 Korean Journal of Clinical Oncology Vol.9 No.1
Purpose: The aim of this study was to assess the impact of laparoscopic vs. conventional open surgery for rectal cancer on clinical and oncologic outcome in a multi-modal setting. Methods: In total, 940 patients who underwent conventional open surgery between February 1995 and October 2007, and 311 patients who underwent laparoscopic surgery between December 2006 and May 2011 were enrolled retrospectively. Oncologic outcomes included 3-year overall survival (OS) and disease free survival, factors affecting them, and risk factors for local and systemic recurrence. Results: No difference was found between laparoscopic surgery and conventional open surgery in terms of OS, disease free survival rate at stages I and II, but the 3-year OS for stage III was different between the groups (70.26% for conventional open surgery vs. 90.14% for laparoscopic surgery; P=0.0002). The 3-year disease free survival rate for stage III was 56.59% for conventional open surgery vs. 56.65% for laparoscopic surgery (P=0.6578). The local recurrence rates were 6.81% vs. 8.47% (conventional open surgery vs. laparoscopic surgery; P=0.4960). The systemic recurrence rates were 15.64% vs. 15.71% (conventional open surgery vs. laparoscopic surgery; P=0.9713). Conclusion: Laparoscopic surgery for rectal cancer showed a similar short-term oncologic outcome to conventional open surgery. This suggests that laparoscopic surgery is an acceptable alternative to conventional open surgery for selected patients with rectal cancer.
류하나(Ha-Na Lyu),박미현(Mi-Hyun Park),홍성길(Seong-Gil Hong),이대영(Dae-Young Lee),한경민(Kyung-Min Han),유종수(Jong-Su Yoo),김세영(Se-Young Kim),노영덕(Young-Duk Rho),백남인(Nam-In Baek) 한국식품과학회 2007 한국식품과학회지 Vol.39 No.6
식품 소재의 국내산 약용식물인 163가지 천연물에서 메탄올로 추출한 시료를 이용하여 in vitro에서 10, 100, 500, 1000 μg/mL의 4가지 농도에서 대식세포 면역 활성을 측정하였다. 그 결과, 42개의 시료에서 면역증진반응을 보였으며, 그 중 20개의 시료는 음성대조군에 대하여 20% 이상 면역 활성을 증진시키는 것으로 측정되었다. 이중 2가지 농도에서 면역증진반응을 보이는 시료는 총 19개, 3가지 농도에서 면역증진효과를 나타내는 시료는 총 3개[골파(Allium schoenoprasmum), 두릅(Aralia elata), 매생이(Capsosiphon fulvescens)]였으며, 특히 마(Dioscorea batatas)는 각 농도에서 활성을 나타내었을 뿐 아니라 양성대조군과 비슷하거나 높은 활성을 나타내어 면역증진활성이 매우 우수한 것으로 나타났다. In this study, the 163 edible plants that are permitted as foods by the Korea Food and Drug Administration, were extracted in methanol. Following extration, their macrophage immunostimulating effects (MIE) were examined using a macrophage from BALB/c mice at four different concentrations of plant extract, such as 10 μg/mL, 100 μg/mL, 500 μg/ mL, and 1,000 μg/mL, in vitro. Forty-two samples significantly showed MIEs. Among them, 20 samples had increased MIEs at higher than 10%, as compared to the negative control group. Nineteen samples had increased MIEs at two of the plant extract concentrations, and 3 samples [Allium schoenoprasmum (chive), Aralia elata (fatsia), Capsosiphon fulvescens (seaweed fulvescens)] had increased MIEs at three of the concentration conditions. In particular, Dioscorea batatas (yam) showed MIEs at all concentrations, as well as slightly higher MIEs as compared to the positive control group. Therefore, Dioscorea batatas was evaluated to be an excellent MIE.
강화도 약쑥(Artemisia princeps Pampanini) Callus로부터 Sterols의 생산
한민우,방면호,유종수,백남인,김세영 경희대학교식량자원개발연구소 2007 硏究論文集 Vol.26 No.1
강화약쑥의 기내배양을 통하여 유효활성물질의 대량생산 가능성을 확인하고자 하였다. Callus 유도는 auxin계의 대표적인 물질인 2mg/ℓ, 2,4-D가 첨가된 고체배지에서 17일 정도에 유도되어 가장 빠른 것으로 나타났으며, 생육도 양호하였다. 또한, 2mg/ℓ, 2,4-D와 sucrose 5g/ℓ 을 첨가한 MS 배지에서 callus의 생육이 가장 좋은 것으로 나타났으며, 배양 10일부터 20일 까지는 완만한 생장을 보였고, 배양 20일후부터 30일 까지 왕성한 생장을 하는 것으로 나타났다. 강화약쑥의 callus 추출물을 TLC분석한 결과 강화약쑥의 전초에서 발견된 steroid 화합물인 β-sitosterol과 daucosterol이 존재한다는 것을 알 수 있었다. 그러나, 식물체와 기내 배양한 세포사이에는 많은 대사물질의 차이를 나타내었고 callus에 존재하는 유용하고 다양한 대사물질을 기내배양을 통하여 대량생산 할 수 있는 가능성을 제시하고 있다. The study was conducted to examine the mass production potential of activie compounds by in vitro culture of Artemisia princeps Pampanini. The plant has been known as a crude medicine and used for treatment of colic pain, cardiotonic and antiphlogostic. The callus was induced and cultured in a agar medium containing 2 mg/ℓ 2,4-D, 5g/ℓ sucrose. TLC analyses of the callus extract showed that it contained β-sitosterol and daucosterol, which were reportedly detected in the leaves of the plant. TLC analyses suggested that there were some differences in metabolites between the callus in vitro and intact plant, yet in vitro culture had a potential for a mass production of secondary metabolites from Artemisia princeps Pampanini.
Canine juvenile cellulites의 진단과 치료 증례
황철용,유종현,강형석,윤화영,한홍율 한국임상수의학회 2002 한국임상수의학회지 Vol.19 No.4
Canine Juvenile cellulitis was diagnosed in 2 puppies hospitalized in Veterinary Medical Teaching Hospital of Seoul National University. Characterized dermatological problems were presents of scale, crust, purlent exudation and alopecia limited on their face. On cytologic examinations of direct impression smears for the lesions, numerous neutrophils and macrophagies were observed. No bacteria and fungus were noted. Treatments with administration of predinisolone and antibiotic orally combined with topical shampoo treatment had good results for the two puppies.