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단보 : 저온 보존 기간에 따른 담자균류의 생존율 비교
유성열 ( Sung Ryul Ryu ),가강현 ( Kang Hyeon Ka ),이봉훈 ( Bong Hun Lee ),박현 ( Hyun Park ),박원철 ( Won Chull Bak ) 한국균학회 2011 韓國菌學會誌 Vol.39 No.2
Short-preservation of basidiomycetes is generally being conducted in slant tubes containing solid medium based on agar. In this study, we investigated the vitality of 28 species and 76 strains preserved on potato dextrose agar (PDA) at 4℃ for 2~7 years. The survival rates of the fungi were 82%, 86%, 94%, 96%, 94%, and 94% for seven, six, five, four, three, and two years old preservation, respectively. The volume of medium in Lentinula edodes showed decrease after 2 years preserved. The pH of preserved medium was 5.42 in 2007 (two years old), but it became nearly neutral as increasing preservation term.
낙엽송 톱밥배지 밀도 및 입자크기에 따른 꽃송이버섯의 재배특성
박현 ( Hyun Park ),유성열 ( Sung Ryul Ryu ),가강현 ( Kang Hyeon Ka ) 한국목재공학회 2011 목재공학 Vol.39 No.1
꽃송이버섯은 다량의 β-glucan을 함유하고 있어서 기능성 버섯으로 재배가 확산되고 있다. 본 연구는 재배공정의 표준화를 위한 기초연구로서, 낙엽송 톱밥배지를 대상으로 밀도와 입자 크기에 따른 균사 생장 및 생산량 차이를 검정하였다. 저밀도(0.68~0.72g/cm3)의 톱밥배지에서 균사 생장의 변이계수는 초기에 40%를 초과하는 높은 값을 나타내었다가 7주차 이후에는 10% 이내로 줄어들었다. 반면, 고밀도(0.80g/cm3)의 경우에는 초기에도 상대적으로 낮은 30% 수준의 변이계수를 나타내며 안정적인 모습을 보였다. 생산량에서는 상품성이 높은 꽃 부분의 회수율을 측정한 결과 0.80g/cm3은 12.2%, 0.76g/cm3은 13.6%, 0.72g/cm3은 13,l%, 그리고 0.68g/cm3은 12.0%로 환산되어 0.76g/cm3가 가장 유리한 것으로 판단되었다. 한편, 1mm 이하의 톱밥을 배제한 경우에는 원기 형성 후 자실체 수확까지 40일 정도 소요되지만, lmm 이하의 톱밥이 섞일 경우는 70일이 소요되었다. 또한 생산량에 있어서도, 꽃 부분만을 고려하면 lmm 이하의 입자를 포함한 경우에는 다른 처리에 비하여 생산량이 적은 것을 확인할 수 있었다. 따라서 낙엽송 톱밥을 이용하여 꽃송이버섯을 재배하기 위해서는 lmm 이하의 톱밥은 제외하고. 0.76g/cm3의 밀도로 배지를 조제하여 사용하는 것을 권장한다. Cauliflower mushroom (Sparassis crispa) is being cultivated as a functional mushroom since the mushroom contains larger amount of β-glucan than other edible or medicinal mushrooms In this study, as a fundamental study for the cultivation process of cauliflower mushroom, we assayed the mycelial growth and the productivity of the mushroom cultivated on the sawdust-based medium made of larch (Larix kaempferi) by 4 kinds of medium densities and 6 kinds of particle sizes. Low densities of sawdust-based media (0.68-0.72g/cm3) showed high variations in mycelial growth at early stage on sawdust-based medium. The coefficient of variance for the mycelial growth decreased from higher than 40% at the 3rd week to lower than 10% at the 7th week. High density of sawdust-based medium (0.80g/cm3) showed relatively lower variation in the mycelial growth at early stage with less than 30% of coefficient of variance, the high density of sawdust-based medium was thought to be quite stable compared to the lower densities of sawdust-based medium. From the viewpoint of mushroom productivity especially for the goods (excluding bottom of fruiting body), 0.76g/cm3 was better than any other densities; the return rates of fruiting body from each medium were 12.2, 13.6, 13.1, and 12.0% for 0.80, 0.76, 0.72, and 0.68g/cm3, respectively. By the way, it took about 40 days for harvesting from primordium formation with the sawdust-based medium excluded the particles less than 1mm, while it took about 70 days with the medium including the particles less than 1mm. The yield from the sawdust-based medium with the particles less than 1mm was also quite less than any other sawdust-based medium especially for the goods Therefore, we recommend that the sawdust-based medium with larch for the cultivation of cauliflower mushroom be prepared as 0.76g/cm3 in medium density with excluding the particles less than 1mm.
자작나무시루뻔버섯(차가버섯)을 접종한 자작나무로부터 버섯의 생장
가강현 ( Kang-hyeon Ka ),전성민 ( Sung-min Jeon ),박현 ( Hyun Park ),이봉훈 ( Bong-hun Lee ),유성열 ( Sung-ryul Ryu ) 한국균학회 2017 韓國菌學會誌 Vol.45 No.3
Chaga mushroom (Inonotus obliquus), which has invaluable medicinal uses, grows only on living trees. To date, it is still harvested from its natural habitat and is not cultivated artificially. We artificially cultivated chaga mushrooms by inoculating its sawdust spawns on Betula platyphylla var. japonica in 2007, and monitored mushroom growth on the inoculated trees for 9 years. The mushrooms grew less than 1 cm per year, with the largest mushroom growing up to 9 cm in the 9 years of study. There was no difference in the growth (diameter at breast height) of trees with viable and non-viable I. obliquus. In conclusion, artificial cultivation of chaga mushroom was successful. Our findings suggest that selection of large B. platyphylla var. japonica as host tree could lead to better I. obliquus productivity. Further improvements of the method are needed to increase the success rate of I. obliquus inoculation.