형질전환 벼 이용 품종 조기개발 체계 확립

원용재,이기환,조준현,고종민,박향미,한창덕,양세준,김순철,남민희,Won, Yong-Jae,Yi, Gi-Hwan,Cho, Jun-Hyeon,Ko, Jong-Min,Park, Hyang-Mi,Han, Chang-Deok,Yang, Sae-Jun,Kim, Soon-Chul,Nam, Min-Hee 한국식물생명공학회 2004 식물생명공학회지 Vol.31 No.1

This study was carried out to establish a new breeding scheme which is connected with conventional breeding method and anther culture method. To develop a herbicide resistant and direct seeding rice, $F_1$ plants were subjected to anther culture and regenerated plants from 5 crosses were studied to confirm the introduction of bar gene. After PCR analysis, we selected 227 plants which were carrying herbicide resistance gene (bar) out of 1,508 regenerated plants from anther culture. Among 169 $A_2$ lines carrying herbicide resistant gene from 5 crosses including YR23235 (Dongjin Ds3(Ba $r^{R}$)/ Milyang165), 42 lines that had superior agronomic characters were selected for further research. Among them, YR23235Acp79 which showed herbicide resistance, direct seeding adaptability and superiority in major agronomic characters was named Milyang 204. This breeding scheme proved that the anther culture of $F_1$ plants crossed between transformant and cultivar or transform ant alone could be utilized in breeding programs for a rapid progeny fixation and development of a variety.y. 형질전환 식물체를 직접 이용하기에는 후대에 유전적인 고정 및 포장검정이라는 문제가 대두되는데, 이를 해결하기 위하여 본 연구는 바스타저항성 유전자가 도입된 벼와 직파적 응성 계통을 교잡한 후 약배양에 의한 형질전환체 고정 등 기존 육종체계와 연계하여 제초제 저항성 직파적응 계통을 조기에 육성할 수 있는 체계를 확립하고자 수행하였다. 제초제 저항성 직파적응 계통을 조기에 육성하고자 1999년 동계에 교배된 동진벼 Ds15(Ba $r^{R}$)/주남벼 등 5조합으로부터 2000년 $F_1$을 양성하고, 이들을 PCR분석으로 제초제 저항성 유전자 bar도입을 확인한 후 약배양을 실시하여 얻어진 1,508개의 재분화 식물체 중 제초제 저항성 유전자가 도입된 169개체 ( $A_2$)에서 채종하였다. 이들을 2001년 포장에 공시하여 42계통을 선발하고,2002년에 동진벼 Ds15 (Ba $r^{R}$)/주남벼 조합에서 수량 등 주요 농업형질이 우수한 7계통을 생산력 검정시험에 공시한 결과 제초제 저항성이면서 직파적응성 등 기타 농업형질이 우수한 YR23235Acp79를 선발하여 밀양 204호로 계통명을 부여하였다. 결과적으로 형질전환체를 이용하여 교배부터 계통선발까지 3년 만에 목적하는 계통을 조기에 육성할 수 있는 생력육종체계가 확립되었다.

중부지역 적응 조생 복합내병성 고품질 벼 ‘중모1023’

원용재(Yong-Jae Won),조영찬(Young-Chan Cho),최임수(Im-Soo Choi),이점호(Jeom-Ho Lee),이상복(Sang-Bok Lee),이정희(Jeong-Heui Lee),오명규(Myung-Kyu Oh),김정주(Jeong-Ju Kim),양창인(Chang-Ihn Yang),안억근(Eok-Keun Ahn),장재기(Jae-Ki Chang 한국육종학회 2015 한국육종학회지 Vol.47 No.3

The ‘Jungmo1023’ is the japonica rice variety developed from a cross between Yeongdeog34 and F1 of Iksan456 and SR21097-B-B-19-2-2 by the rice breeding team at NICS in 2012. The heading date of ‘Jungmo1023’ is August 3 in middle plain area and it is six days earlier than ‘Hwaseong’. ‘Jungmo1023’ has 80 cm of culm length which is 4 cm shorter than those of ‘Hwaseong’ and 107 spikelet per panicle. The premature heading rate of ‘Jungmo1023’ was 3.3%. It showed resistance to blast, bacterial blight (K1, K2, K3 race) and stripe virus, but susceptibility to K3a race of bacterial blight, dwarf and black streak dwarf viruses and planthoppers. The milled rice of this variety exhibits translucent and medium short grain shape. It has better palatability index of cooked rice (0.24) and lower amylose content (18.0%) than that of ‘Hwaseong’. The characteristics related to grain milling were better than those of ‘Hwaseong’, especially head rice milling recovery ratio and head rice ratio (96.2%). ‘Jungmo1023’ showed 4.56 MT/ha of milled rice productivity at 7 sites in ordinary cultivation. However, ‘Jungmo1023’ showed early cultivation culture adaptability because of 98% of its yield potential (4.99 MT/ha) compared to high yielding early-maturing variety ‘Jopyeong’. ‘Jungmo1023’ could be adaptable to the middle plain, mid-mountainous and north-east coastal area in Korea (Registration No. 5115).

복합내병성 가공밥 적성 벼 ‘미소미’의 농업적 특성

원용재(Yong-Jae Won),양창인(Chang-Ihn Yang),조영찬(Young-Chan Cho),최임수(Im-Soo Choi),김명기(Myeong-Ki Kim),정응기(Eung-Gi Jeong),이점호(Jeom-Ho Lee),이상복(Sang-Bok Lee),백만기(Man-Kee Baek),홍하철(Ha-Cheol Hong),안억근(Eok-Keun Ah 한국육종학회 2016 한국육종학회지 Vol.48 No.3

The Misomi is a rice variety derived from a cross between ‘Sobi’ which has adaptability of low fertilizer cultivation and SR21110 (Ilpum/Jinbu19) by the rice breeding team at National Institute of Crop Science, RDA in 2013. The heading date of ‘Misomi’ is August 14 and six days later than check variety, ‘Hwaseong’. It has 83 cm of culm length and 121 spikelets per panicle. It showed weaker cold tolerance than that of ‘Hwaseong’ during exposed to cold stress. ‘Misomi’ showed resistance to blast disease, K1, K2 and K3 race of bacterial blight and stripe virus, but susceptibility to K3a race, other viruses and planthoppers. The milled rice of this variety exhibits translucent, clear non-glutinous endosperm and short grain shape. It has low protein content (6.2%) and similar amylose content (19.4%) to that of ‘Hwaseong’. ‘Misomi’ showed better palatability index of cooked rice than that of ‘Hwaseong’. Particulary, ‘Misomi’ showed excellent processing adaptability than ‘Chucheong’ and ‘Hwaseong’ on palatability index of cold and aseptic cooked rice. Its milled rice recovery rate is similar to those of ‘Hwaseong’. However, whole grain rate of milled rice is higher than that of ‘Hwaseong’. Misomi has 5.68 MT/ha of milled rice productivity in ordinary cultivation, which was 12% higher than that of ‘Hwaseong’. Misomi could be adaptable to the middle plain area and Midwest coast area in Korea (Grant No. 5635).

가공용 쌀 품종 및 가공밥 이용기술 개발

원용재(Yong-Jae Won),오세관(Sea-Kwan Oh) 한국식품과학회 2014 식품과학과 산업 Vol.47 No.2

노래방 리모콘 인터페이스 개선방안에 대하여

원용재(Yongjae Won),김형식(Hyungsik Kim),임성범(Sungbum Lim),김신우(ShinWoo Kim) 한국HCI학회 2014 한국HCI학회 학술대회 Vol.2014 No.2

1991년 한국에 처음 노래방이 등장한 이후 수많은 노래방이 생겨나기 시작했고, 노래방 문화는 한국 사회의 유흥문화에 핵심적인 역할을 담당하고 있다. 하지만 노래방이 대중적인 인기를 얻고 다양하게 변신을 하고 있는 반면 노래방 기계를 조작 하는 리모컨의 인터페이스는 여전히 복잡하고 사용하기 불편하다. 특히 자주 사용하는 [취소]버튼은 그 기능이 모호한 반면 자주 사용하지 않는 다양한 버튼들로 인해 많은 사용자들은 혼란을 겪게 된다. 본 연구는 설문조사 및 면접을 통해 노래방 리모컨 사용 시 발생하는 어려움에 대해 조사하였다. 그 결과 사용자들이 주로 겪고 있는 문제는 [취소]버튼의 모호성과 잘 사용하지 않는 다수의 기능 버튼 때문이라는 예측을 확인하였다. 설문결과를 바탕으로 [현재곡 취소]와 [예약곡 취소]를 하나의 버튼으로 통합하여 사용자들의 빈번한 오류인‘예약곡"을 취소하려다 현재곡을 취소하는 실수’를 줄일 수 있는 대안을 제시하였다. 더불어 undo 기능의 추가와 자주 사용하지 않는 기능들의 재분류에 대해 논의하였다. Since its initial introduction of karaoke to Korea in 1991, a large number of karaoke sprang up many places and karaoke culture has become one of core components in Korean entertainment night life. However whereas karaoke gained mass popularity and changed into different forms, UI of remote controller that operates karaoke machine is still complex and confusing. In particular, the function of frequently used ‘cancel’ button is at times ambiguous and users are confused by a number of rarely used buttons. This research investigated difficulties involved in the use of karaoke remote controller using questionnaire and interview. The results confirmed our prediction that the main UI problem was ambiguity of cancel button and a number of messy buttons rarely used. Based on survey results, alternative button layout was proposed which integrates current song and reserved song cancel buttons in order to reduce frequent error where users abort the current song when intended to cancel reserved ones. Furthermore, addition of ‘undo’ function and rearrangement of rarely used buttons are discussed.

강원 영서지역에서 겨울 및 여름 사료작물 이모작 시 생산성

손범영,원용재,김성국,김민태,Son, Beom Young,Won, Yong Jae,Kim, Sung Kook,Kim, Min Tae 한국초지조사료학회 2021 한국초지조사료학회지 Vol.41 No.2

본 시험은 강원 영서지역에서 겨울사료작물(IRG 및 트리티케일)과 여름사료작물(사료용 벼) 이모작 시 양질 조사료의 최대 수량 생산 작목조합을 알아보기 위해 수행하였다. 겨울사료작물의 건물률은 IRG '코윈어리'가 평균 29.6%로 트리티케일 '조성'의 평균 35.5%보다 낮았다. 평균 생체수량은 '코윈어리'가 2,662kg/10a, '조성'이 2,836kg/10a로 두 작목 간 통계적으로 유의적인 차이가 없었다. 평균 건물수량은 트리티케일 '조성(996kg/10a)'이 IRG '코윈어리'(696kg/10a)보다 많았다. 여름사료작물인 사료용 벼의 건물률은 '조농'이 평균 34.5%, '조우'가 평균 35.0%로 유의적인 차이가 없었다. 평균 생체수량은 '조우'가 5,367kg/10a로 '조농'의 3,966kg/10a보다 많았다. 또한 평균 건물수량도 '조우(1,936kg/10a)'가 '조농(1,433kg/10a)'보다 많았다. 겨울사료작물인 트리티케일 '조성'을 10월 중순에 파종하여 이듬해 5월 하순에 수확한 후 여름사료작물인 사료용 벼 '조우'를 6월 상순에 이앙하는 조합으로 2,982kg/10a의 최대 건물 생산량을 얻을 수 있었다. In order to establish optimal double cropping system for getting the maximum annual productivity, we investigated annual fresh and dry yields of winter forage crops (WFC), Italian ryegrass (IRG) 'Kowinearly', triticale 'Joseong' and summer forage crop (SFC), 'Jonong' and 'Jowoo' as whole crop silage rice in the paddy field of Yeongseo of Gangwon Province. The double cropping of each crops was applied with 2 standard cultivation method of WFC and SFC from 2018 to 2020. For the WFC, the average percentage of dry matter (29.6%) of IRG 'Kowinearly' was lower than that (35.5%) of triticale 'Joseong'. The average fresh matter yield of IR G 'Kowinearly' was 2,662kg/10a that there was no significant difference from the 2,836kg/10a of triticale 'Joseong'. The average dry matter yield (996kg/10a) of triticale 'Joseong' was more than that (696kg/10a) of IRG 'Kowinearly'. For the summer forage crops, the average percentage of dry matter of whole crop silage rice, 'Jonong' was 34.5% that there was no significant difference from the 35.0% of 'Jowoo'. The average fresh matter yield (5,367kg/10a) of 'Jowoo' was more than that (3,966kg/10a) of 'Jonong'. And the average dry matter yield (1,936kg/10a) of 'Jowoo' was more than that (1,433kg/10a) of 'Jonong'. The total maximum dry matter yield was 2,982kg/10a with the combination of the WFC triticale, 'Joseong' and the SFC whole crop silage rice, 'Jowoo'. In conclusion, the combination of crops that can obtain the maximum yield of high quality forage for double cropping is sowing the WFC triticale, 'Joseong' in mid-October, harvesting 'Joseong' around the end of May and then SFC whole crop silage rice, 'Jowoo', to be transplanted in early June.

벼 약배양에 효과적인 배지조성 및 저온처리 방법

이기환,원용재,고종민,박향미,조준현,오병근,양세준,김순철,남민희,Yi, Gi-Hwan,Won, Yong-Jae,Ko, Jong-Min,Park, Hyang-Mi,Cho, Jun-Hyeon,Oh, Byeong-Geun,Yang, Sae-Jun,Kim, Soon-Chul,Nam, Min-Hee 한국식물생명공학회 2003 식물생명공학회지 Vol.30 No.4

In spite of potential benefits of anther culture, low productivity of plant regeneration in some genotypes; e.g. tonsil and indica rice, is one of the major obstacles for practical use of anther culture. This study was conducted to improve cold shock method and carbohydrate source for increasing the efficiency of anther culture in rice. The most common carbon source, sucrose was replaced to maltose, which has two molecules of glucose. Maltose increased callus induction 1.4-to 1.8-fold higher in japonica rice, 3.2-to 11.6-fold in tongil types and 2.7-fold in indica rice IR50. Callus induction was increased from 0.2% to 12.5% in maltose medium compared to the medium supplemented with sucrose plus glucose in indica rice "Tetep". A simple procedure of vacuum packaging of panicles during cold shock treatment prolonged not only anther viability more than 15 days but also increased callus induction more than 2-fold compared to open-air storage (conventional method). Combining of above two methods, callus induction was increased 28 to 56% in japonica, 13 to 33% in tonsil type and 12 to 31% in indica rice. Plant regeneration was increased 14 to 35% in japonica, 10 to 20% in tonsil and 4 to 15% in indica rice, respectively.

형질전환 제초제저항성 벼 밀양 204호의 농업적 특성

정응기,이기환,원용재,박향미,전남수,최준호,구연충,한창덕,은무영,김태산,남민희,Jeong Eung-Gi,Yi Gi-Hwan,Won Yong-Jea,Park Hyang-Mi,Cheon Nam-Soo,Choi Jun-Ho,Ku Yeon-Chung,Han Chang-Deok,Eun Mu-Yeong,Kim Tae-Sas,Nam Min-Hee 한국식물생명공학회 2005 식물생명공학회지 Vol.32 No.2

밀양 204호는 작물과학원 영남농업연구소 생명공학연구팀이 직파적응 제초제저항성 벼 품종을 육성할 목적으로 '98년 bar 유전자를 동진벼에 아그로박테리움법으로 형질 전환하여 제초제저항성 벼를 양성하였다. '98/'99년 동계에 단간 내도복 양질인 주남벼를 인공교배하여 약배양 등 육종프로그램에 적용하여 우량계통을 선발하고 밀양 204호로 계통명을 부여하였다. GM 벼와 Non-GM 벼의 농업적 특성을 UPOV 및 국립종자관리소의 품종등록 기준에 따른 농업적특성 중 차이가 있었던 것은 엽색도, 지엽의 형태, 간장, 수당립수 등 이었다. This study was conducted to investigated the major characteristics of genetically modified rice of 'Milyang 204' originated from Dongjinbyeo compared to a non-transgenic rice varieties Dongjinbyeo and Jun-ambyeo. Basta resistant transgenic rice lines carrying bar gene produced by the Yeongnam Agricultural Research Institute were evaluated for their agronomic characters. The transgenic Japonica rice of 'Milyang 204' showed inferior phenotypic traits compared to a non-transgenic rice variety Dongjinbyeo and Junambyeo. On the basis of UPOV (Union Internationale Pour la Protaection des Obtentions Vegetables) and NSMO(National Seed Management Office) the transgenic 'Milyang 204' showed difference in some traits out of some agronomic traits, such as leaf color, angle of flag leaf, number of spikelets, culm length, white core and white belly compared to the non-transgenic varieties rice.

형질전환 제초제저항성 벼 밀양 204호의 농업적 특성

정응기,이기환,원용재,박향미,전남수,최준호,구연충,한창덕,은무영,김태산,남민희 Plant molecular biology and biotechnology research 2005 Plant molecular biology and biotechnology research Vol.2005 No.

This study was conducted to investigated the major characteristics of genetically modified rice of "Milyang 204" originated from Dongjinbyeo compared to a non-transgenic rice varieties Dongjinbyeo and Junambyeo. Basta resistant transgenic rice lines carrying bar gene produced by the Yeongnam Agricultural Research Institute were evaluated for their agronomic characters. The transgenic Japonica rice of "Milyang 204" showed inferior phenotypic traits compared to a non-transgenic rice variety Dongjinbyeo and Junambyeo. On the basis of UPOV (Union Internationale Pour la Protaection des Obtentions Vegetables) and NSMO(National Seed Management Office) the transgenic "Milyang 204" showed difference in some traits out of some agronomic traits, such as leaf color, angle of flag leaf, number of spikelets, culm length, white core and white belly compared to the nontransgenic varieties rice.

조생종 벼의 평야지 재배에 따른 등숙 온도 차이에 의한 전분구조 및 종자 저장단백질 특성 연구

곽지은,이점식,원용재,박향미,곽강수,김미정,이춘기,김선림,윤미라,Kwak, Jieun,Lee, Jeom-Sig,Won, Yong-Jae,Park, Hyang-Mee,Kwak, Kang-Su,Kim, Mi-Jung,Lee, Choon-Ki,Kim, Sun-Lim,Yoon, Mi-Ra 한국작물학회 2018 한국작물학회지 Vol.63 No.2

This study was performed to understand the effects of filling stage temperature on the characteristics of starch and storage protein and the quality of rice grains. Eight early maturing rice varieties were cultivated in Cheolweon (latitude $38^{\circ}15^{\prime}N$) and Suwon (latitude $37^{\circ}16^{\prime}N$) areas in Korea. Rice grown in Suwon, with relatively high ripening period temperatures, showed significantly reduced head rice ratio and eating qualities, higher protein and lower amylose contents than rice grown in Cheolweon. In rice that ripened under high temperature conditions, the starch contained significantly less short-chain amylopectin (DP < 12) but more intermediate- (DP 13-24) and long- (DP > 25) chain amlylopectin compared to rice that ripened under normal conditions. In addition, the electrophoretic pattern of rice storage protein under high- temperature conditions revealed decreased prolamin and increased glutelin contents.