단방조충(單房條蟲)의 형태(形態)와 생활사(生活史)에 관(關)한 연구(硏究)

오문유,김오남,한방근,김영홍,Oh, Moon You,Kim, O Nam,Han, Bang Keun,Kim, Young Hong 대한수의학회 1976 大韓獸醫學會誌 Vol.16 No.2

The purposes of this study were to define the life cycle of Echinococcus and its species and subspecies in Korea. The results were as followings: 1. The patent period day of Echinococcus granulosus was 50 days. 2. The life cycle of Echinococcus granulosus was dog-cattle-dog, as cattle-dog originating adult. 3. The sine of the adult worm was measured from 2.2mm to 6.2mm, but most of them was between 3.0mm and 4.0mm (40% of all). 4. Although the number of segments was from three to five proglottids, most of them was 3 proglottids(76.7%), as immature, mature and gravid, respectively. 5. The habitat of the adult worm was recognized as duodenum or pyloric region in the dog. 6. According to the results above, we could be proved that the hydatid cysts of he Echinococcus discovered in Jeju-do, was Echinococcus granulosus granulosus.

SSCP 분석을 이용한 제주도와 대마도 재래마의 미토콘드리아 DNA 다형현상

오문유(Moon You Oh),정용환(Yong Hwan Jung) 한국유전학회 2001 Genes & Genomics Vol.23 No.1

N/A The mitochondrial DNA (mtDNA) D-loop region was amplified form Cheju (Korea) Tsushima (Japan) native horses by polymerase chain reaction (PCR). The oligonucleotide primers used to amplify the equine mtDNA D-loop region was designed using tRNA-Thr and tRNA-Phe sequences in mtDNA regions highly conserved in many other animal species. There were 1,127 base pairs (bp) in the D-loop region. The middle of the region contained 20 tandem repeats of an 8-bp equine-specific sequence, TGTGCACC. We designed primers for PCR-mediated single-strand conformation polymorphism (SSCP) analysis that amplified a 322 bp fragment, which contained the most variable region according to our sequence data. SSCP analysis of denatured amplification products was carried out by polyacrylamide (8%) gel electrophoresis followed by silver staining. The SSCP analysis identified four different band patterns (A, B, C, and D) and comparison of these four nucleotide sequences identified 34 base substitutions. The A type (90%) was found in most of the Cheju native horses, while the D type (96%) in nearly all the Tsushima native horses. These results show that SSCP analysis of the D-loop region in useful for detecting polymorphism and verifying the lineage of Cheju and Tsushima native horses.

두릅나무과 식물의 SOD 활성과 광계 2 의 광화학적 효율에 미치는 온도 스트레스와 Paraquat 의 영향

오순자(Soon Ja Oh),고정군(Jung Goon Koh),김응식(Eung Sik Kim),오문유(Moon You Oh),고석찬(Suck Chan Koh) 한국환경생물학회 1999 환경생물 : 환경생물학회지 Vol.17 No.2

The effects of temperature stress and paraquat on the superoxide dismutase(SOD) activity and the photochemical efficiency of photosystem Ⅱ were studied in the leaves of Araliaceae plants. The SOD activity of Acanthopanax koreanum leaf discs increased at 4℃ and 28℃, and increased significantly at 4℃, 28℃ and 35℃ in the presence of paraquat. However, the SOD activity of Dendropanax morbifera leaf discs decreased at 4℃, 28℃ and 35℃ regardless of paraquat treatment. The photochemical efficiency of photosystem Ⅱ, Fv/Fm, of leaf discs of A. koreanum and D. morbifera fell remarkable at 35℃. IN the presence of paraquat, the Fv/Fm values fell slightly at 4℃ in A. koreanum leaf discs and at 35℃, in D. morbifera leaf discs. These results indicate that A. koreanum plants are more resistant to temperature stress or oxidative stress than D. morbifera plants although their photochemical efiiciency falls slightly at 4℃ in the presence of paraquat.

한라산 구상나무 잎의 엽록소형광의 일변화와 계절적 변화

오순자(Soon Ja Oh),고정군(Jung Gon Koh),김응식(Eung Sik Kim),오문유(Moon You Oh),고석찬(Suk Chan Koh) 한국환경생물학회 2001 환경생물 : 환경생물학회지 Vol.19 No.1

N/A Chlorophyll fluorescence of needles of Korean fir(Abies koreana) plants and environmental factors of their natural habitat were investigated in order to obtain the information for environmental adaptation and conservation of Korean fir plants. The photochemical efficiency of photosystem Ⅱ, Fv/Fm, of Korean fir needles was significantly low(0.19∼0.36) in the winter, whereas it was high(0.8∼0.86) in the summer. The Fv/Fm value of the winter was slightly higher at mid-day than at dawn. suggesting that mid-day environmental conditions of the winter were favorable on needles of Korean fir plants. In contrast, the mid-day Fv/Fm value of the summer maintained high(around 0.8). It indicates taht mid-day environmental conditions of the summer did not induce photodamage, although it caused a slight decrease in the Fv/Fm values. The non-photochemical fluorescence quenching(NPQ) of Korean fir needles was very low(0∼0.01) all through the day in the winter. However, it was high(0.76) at mid-day in the summer. These results suggest that Korean fir plants have a system for the protection of PS Ⅱ from mid-day environmental stresses of the summer. In the winter, the Fv/Fmvalues were positively correlated with temperature, light intensity and relative humidity, although NPQ values showed no correlation with any of them. In the summer, the Fv/Fm values were positively correlated with relative humidity but negatively correlated with temperature and light intensity. These results indicate that increase of tempera-ture, light intensity and relative humidity lead to promotion of the photochemical efficiency in the winter and high temperature and light intensity may cause photoinhibition in the summer.

국내 말로부터 비폐렴바이러스의 분리 및 면역원성에 관한 연구 II. 국내 분리 말 비폐렴바이러스의 특성 및 면역원성 조사

조길재,김봉환,이두식,오문유,고미희,Cho, Gil-jae,Kim, Bong-hwan,Lee, Du-sik,Oh, Moon-you,Ko, Mi-hee 대한수의학회 1995 大韓獸醫學會誌 Vol.35 No.4

The study was carried out to characterize the properties of Korean isolates of EHV from aborted fetuses and determine envelope protein profiles. The results obtained were summarized as follows; 1. Two strains of EHV was isolated from 2 liver samples among 10 aborted fetuses from which the virus isolation was attempted. 2. Morphological and some enzymatic properties of the Korean isolates of EHV which was designated as $LC_1$ and $LC_2$ was identical to those of a reference strain of Australia-N of EHV-1. The Korean isolates of EHV could be propagated on ED cell culture and they formed typical plaques 1 to 2 days after infection in the ED cells from which typical cuboidal particles of 150~170 nm diameter herpesvirus were observed. The virus could be detected specifically from neucleus and cytoplasm of infected cells by flourescent antibody technique using FITC labelled anti-Aust IV(EHV-1) antiserum. The Korean isolates, $LC_1$ and $LC_2$ were specifically neutralized by anti Aust IV antiserum and reacted positively to CELISA. 3. The structural polypeptides of purified enveloped virions of $LC_1$ and $LC_2$ isolates of EHV were determined by SDS-polyacrylamide gel electrophoresis to identify the envelope glycoproteins. $LC_1$ and $LC_2$ strains revealed 14 glycoproteins ranging in molecular weight from 190 kD to 31 kD while 17 structural proteins of Aust IV(EHV-1), of which 14 were identical to those of $LC_1$ and $LC_2$, were identified. Upon immunoblotting by rabbit antiserum against EHV isolates and EHV-1(Aust IV), 4 immunogenic proteins of $LC_1$ and $LC_2$ were 135 kD, 88 kD, 64 kD and 59 kD, of which 135 kD, 88 kD and 64 kD proteins were also found in Aust IV(EHV-1).

한국멧토끼 ZFX와 ZFY 유전자의 성별 이형성과 분자 성판별

한상현,조인철,이성수,오문유,오홍식,Han, Sang-Hyun,Cho, In-Cheol,Lee, Sung-Soo,Oh, Moon-You,Oh, Hong-Shik 한국생명과학회 2007 생명과학회지 Vol.17 No.3

우리나라에 분포하는 멧토끼 (Lepus coreanus)의 성판별을 위한 분자 표지자를 개발하기 위하여, X, Y 염색체간 상동인 ZFX와 ZFY 유전자들의 성별 이형성에 초점을 맞추어 본 연구를 수행하였다. ZFX와 ZFY 유전자의 인트론 7 영역은 멧토끼의 암수가 구분되는 증폭 양상을 나타내었다. 인트론 7의 길이는 각각 ZFX에서 538, ZFY에서 233-bp로 확인되었다. 특히, ZFX의 인트론 7에서는 RNA-매개성 전위인자 중 한 종이며 토끼의 유전체에서 빈번하게 관찰되는 CSINE2와 유사한 반복서열이 발견되었다. 반면, 반복서열은 ZFY의 인트론 7에서는 관찰되지 않았다. ZFX와 ZFY 유전자의 인트론 7에서 확인된 길이의 차이에 근거하여 중합효소연쇄반응 기법을 이용한 유전자 성판별을 수행하였다. 시험에 이용된 모든 DNA시료들은 ZFX에서 증폭된 공통의 밴드를 가지고 있었다. 이에 반해, 멧토끼 수컷 DNA들은 각각 ZFX와 ZFY에서 증폭된 두 개의 구분되는 밴드들을 나타내었다. ZFX-ZFY 유전자·성판별 결과는 표현형 성별 정보뿐만 아니라 수컷-특이적인 SRY 유전자의 증폭양상과도 일치한 결과와도 정확히 일치하였다. 이상의 결과들은 멧토끼에서 ZFX와 ZFY의 인트론 7 영역간의 성별 이형성은 유전자 성판별을 위한 유용한 유전자 표지자가 될 것으로 사료된다. This study was performed to develop the molecular marker for sex determination of hare (Lepus coreanus) distributed in Korea which focused on sexual dimorphism between X and Y chromosomal homologous genes, zinc finger-X (ZFX) and -Y (ZFY). The intron 7 regions of ZFX and ZFY genes exhibited differential amplification patterns between male and female hares. The lengths of intron 7 region of ZFX and ZFY genes were 538 and 233-bp, respectively. Especially, the ZFX intron 7 contained a repetitive sequence identified as member of RNA-mediated transposable elements which was similar to CSINE2 commonly found in the rabbit genome. However, it was not present in intron 7 of ZFY gene. The molecular sex typing by polymerase chain reaction (PCR) was also carried out to determine the sex of hare based on difference in lengths between the intron 7 regions of ZFX and ZFY genes. All DNA samples tested had common band amplified from ZFX. However, the male hare DNAs had two distinct bands which amplified from ZFX and ZFY genes, respectively. The results from ZFX-ZFY PCR sex typing were identical to those from phenotypic investigation and from amplification patterns using male-specific sex determining region Y (SRY) gene as well. Finally, this study suggested that the sexual dimorphism between intron 7 regions of ZFX and ZFY could be useful genetic marker to determine sex of hare.

흰쥐 중추신경계내 난소로 투사하는 미주신경로에 관한 연구

김명주(Myeong-Joo Kim),장명세(Myeong-Se Chang),고미희(Mi-Hee Ko),노해숙(Hae-Sook Noh),조해영(He-Young Cho),오문유(Moon-You Oh),이봉희(Bong-Hee Lee) 대한체질인류학회 2000 해부·생물인류학 (Anat Biol Anthropol) Vol.13 No.1

본 실험은 부교감신경의 하나인 미주신경이 난소를 지배하는 신경으로 관여하고 있는지를 pseudora. bies 바이러스를 이용하여 난소신경로와 난소주사후 미주신경정단을 통하여 조사한 연구 보고이다. 이를 위하여 Sprague Dawley계 암흰쥐를 대상으로 pseudorabies 바이러스를 난소에 주사한 무리와 난소 주사후 미주신경을 정단한 우리에서 뇌를 적출하여 pseudorabies 바이러스에 대한 연역조직화학 염색을 시행하여 비교하였다. 본 실험결과 미주신경 중추신경로내의 상위신경핵들이 pseudorabies 바이러스에 대하여 양성반응이 줄어들었거나 관창되지 않는 차이를 보였다 즉 적색핵, 종말판혈관기판, 줄무늬체, 침상핵과 이마엽겉질은 부분적으호 난소의 미주신경로에 관여하고 있으며 미주신경동쭉핵, 고립로핵, 최후영역, 청색반정, 팔옆핵, 코리케퓨즈핵 , 흑색질 및 시각교차위핵은 양성반응이 관찰되지 않아 미주신경으로 투사하는 부교감신경핵으로 조사되었다.

흰쥐 뇌에서 속후각겉질 손상 후 해마로 투사하는 콜린성 신경로의 변화에 관한 연구

고미희(Mi-Hee Ko),김대성(Dae-Sung Kim),변경희(Kyung-Hee Byun),김재우(Jae-Woo Kim),김명주(Myeong-Ju Kim),오문유(Moon-You Oh),이봉희(Bong-Hee Lee) 대한해부학회 2003 Anatomy & Cell Biology Vol.36 No.1

흰쥐 뇌에서 해마로 투사하는 신경로와 콜린성 신경로를 구축한 뒤 속후각겉질 손상 후에 해마로 투사되는 두 신경로 의 형태학적인 변화를 조사하기 위하여 pseudorabies 바이러스의 Bartha stain을 신경로 추적자로 이용하여 다음과 같은 결과를 얻었다. 안쪽격벽핵 (medial septal nucleus), 줄무늬침상핵 (bed nucleus stria terminalis) 등 10여 핵에서 양성반응이 나타나 해마로 투사하는 신경로를 구축하였으며 무과립 섬겉질 (agranular insular cortex), 중심편도핵 (central amygdala)등의 여러 신경핵들이 해마로 투사하는 콜린성 신경로를 구성하는 신경핵으로 조사되었다. 이 중 신경핵의 종류에 따라 이중양성반응 정도에는 차이를 보여 콜린성 신경로는 신경핵에 따른 차이를 보였다. 속후각겉질 손상 후 10일과 30일에 해마로 투사하는 신경로와 콜린성 신경로의 형태학적 변화를 조사한 결과 내측안쪽무릎핵 (medial geniculate complex, medial part)과 내측배쪽무릎핵 (medial geniculate complex, ventral part) 및 시상(thalamus)등 일부 신경핵에서 양성반응을 보이는 신경세포들의 수가 현저히 감소하였다. 이상의 결과를 종합해 볼 때 Alzheimer’s 질환 모델인 속후각겉질 손상 흰쥐에서 신경로 구성 세포, 콜린성 신경세포 및 신경핵들이 감소되어 치매시의 신경손상이 신경로를 따라 일어 나며 신경세포 수의 감소와 신경전달물질의 감소가 동반 될 것으로 사료된다. The hippocampus is known as involved in learning and memory functions and the entorhinal cortex plays a crucial role as a gateway connecting the several areas and hippocampal formation. Entorhinal cortex lesions have been employed in numerous studies as the Alzheimer’s disease model. The purpose of this study were to identify the CNS hippocampal and cholinergic pathway and to investigate the morphological changes of the hippocampal cholinergic innervations by using the Pseudorabies virus injection into the hippocampus after entorhinal cortex lesions. The pseudorabies virus and double labelled neurons (ChAT and PRV) were distributed at several different nuclei including agranular insular cortex, bed nucleus of stria terminalis, central amygdala, globus pallidus, lateral segment, lateral hypothalamic area, laterodorsal tegmental nucleus, medial septal nucleus, mesencephalic reticular nucleus, periaqueductal gray matter and substantia innominata The morphological changes were observed in the hippocampal cholinergic innervation after entorhinal cortex lesions. These data suggested that the hippocampal cholinergic innervation showed morphological changes throughout the whole brain areas after entorhinal cortex lesion.

濟州島人의 遺傳學的 硏究Ⅱ : 色感異商者의 頻度에 대하여 On the Frequency of Color Defects

吳文儒 제주대학교 1979 논문집 Vol.11 No.-

I studied on the frequency of color defects in children of some primary schools with Ishihara plates, as one of the follow-up studies on the Jejudonian population genetics. The results are as follows: 1. The frequency of color defects in male was 3.417, and female 0.116. The color defects in Jeju-do showed lower than that in Seoul. 2. The color defects being classified into two groups, protanoids and deuetranoids, the frequency of male protanoids was 0.788, and that of deuteranoids was 2.628%. The frequency of female protanoids was nothing, and 0.116% was the frequency of frmale deuteranoids. The deuteranoids showed higher than the protanoids in both male and female. 3. The frequency of color defects in Jeju-do was the lowest among Seoul and Japan.

濟州道 雜犬의 繁殖生理에 關한 硏究 : 發情 持續期間에 對하여

吳文儒,金永洪,玄寬宗 제주대학교 1976 논문집 Vol.8 No.-

An observation was made on the duration of estrus and the blood values during the estrous cycles in 15 mongrel bitches. The results obtianed were as follows ; 1. The length of proestrus was reckoned up the time from first appearance of blood in the vaginal smears to first acceptance of the male; varied 4 to 12 days (mean±S.D.=7.8±2.6 days). 2. The duration of estrus was determined by the acceptance test following the days between first and last acceptance; varied from 4 to 13 days (8.1±2.6 days). 3. The duration of visible bieeding out of vigina varied from 12 to 23 days (16.9±2.6days). 4. The seasonal occurrence of estrus did not exhibit. 5. During proestrus and estrus, the erythrocyte numbers increased 5-6% above those of established for beagle in anestrus. No significant variation in total leucocyte numbers was noted during the estrous cycles.