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조현주 ( Hyun Joo Cho ),이현경 ( Hyun Kyung Lee ),김동석 ( Dong Seok Kim ),박경찬 ( Kyoung Chan Park ),장민열 ( Min Youl Chang ),김진준 ( Jin Jun Kim ),이천구 ( Cheon Koo Lee ),오달균 ( Dahl Kyun Oh ) 한국조직공학·재생의학회 2007 조직공학과 재생의학 Vol.4 No.3
In this study, the effects of hGH were analyzed by using living skin equivalents(LSEs) models. In monolayer culture of keratinocytes, hGH was not cytotoxic to cells in the range of 0.0005~0.5%. However, hGH inhibit keratinocytes proliferation in all tested concentrations. In fibroblasts, hGH was slightly toxic to fibroblasts at 0.5% concentration. However, hGH stimulate fibroblasts proliferation at the concentrations between 0.005~0.05%. These results showed that hGH is not cytotoxic to epidermal cells up to 0.5% concentration. But, it can be said that different pathways are mediated by hGH in keratinocytes and fibroblasts. Then, the effects of hGH were tested using LSE models. For this study, 0.05% concentration of hGH was used. It is thought that epidermis in LSE is hyperproliferative. Thus, parakeratotic epidermis is observed and granular layer is not well formed. To suppress the hyperproliferative potential of LSEs, high concentration of rh-EGF(10 ng/ml) was usually added in the preparation of LSEs. In this study, 0.05% hGH was added and epidermal thickness of hGH treated model looked slightly thinner than that of rh-EGF models. Interestingly, parakeratosis was not observed in both rh-EGF and hGH models. Thus, it can be said that rh-EGF and hGH can control the hyper-proliferation of keratinocytes in the reconstruction of SEs. In the reconstruction of epidermis, basement membrane formation is also very important. Results showed that α6 integrin and ß1 integrin expression is elevated in hGH models. Furthermore, involucrin expression was evident at upper epidermis. However, expression of PCNA and p63 was not different compared to other models. Results suggest that hGH promote the formation of basement membrane and differentiation.
혈액응고 제8인자 유전자 내부의 BclI, Xbal RFLP 와 St14 VNTR 에 나타나는 다형인자의 빈도추정과 A 형 혈우병 8가계의 연관 분석
문홍모,김장성,오달균,강신혜 한국유전학회 1993 Genes & Genomics Vol.15 No.4
With the acid of PCR, we used two intragenic factor VIII gene RFLP markers(BclI-intron 18 and XbaI-intron 22) and one extragenic marker(St 14 VNTR) to estimate allele frequencies of these markers from 100 Korean individuals and to analyze eight Korean hemophilia A families. Allele frequencies for the presence of the enzyme sites and heterozygosity expectations were 86 and 24% for BclI-intron 18 RFLP marker, and 62% and 47% for XbaI-intron 22 RFLP marker, respectively. When BclI-intron 18 was homozygous, 31.5% of women was predicted to be heterozygous for XbaI-intron 22. Number of repeats in St14 VNTR loci from Korean males differed from those previously reported for Caucasian males. 68% of Korean women was estimated to be heterozygous for St14 VNTR marker. Analysis of hemophilia A families with BclI-RFLP and St14 VNTR proved to be informative for six out of eight families tested.