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U2OS 골육종 세포주의 세포자멸사에서 MMP억제제와 Doxorubicin 작용의 비교연구
문정석,염범우,Moon, Jeong-Seok,Yeom, Bum-Woo 대한근골격종양학회 2007 대한골관절종양학회지 Vol.13 No.2
목적: wild-type p53 단백질을 가지고 있는 U2OS 인간 골육종 세포주의 세포자멸사에서 MMP억제제와 doxorubicin의 작용을 비교하고자 하였다. 대상 및 방법: 배양한U2OS 세포주에 Doxorubicin, MMP억제제(MMPI III)를 따로 투여한 것과 두 약물을 동시에 투여한 것을 비교하였다. 또한 Doxorubicin의 작용이 Fas/FasL 경로를 통해 유발되는지 알아보기 위해 Fas 중화항체를 doxorubicin과 함께 투여하였다. 약물을 처리하여 배양한 세포에서 발생한 세포자멸사와 세포괴사를 확인하기 위해서 유세포 검사를 시행하였다. 결과: Doxorubicin을 처리한 세포가 처리하지 않은 세포보다 약물 농도에서 더 많은 세포괴사(p=0.000)를 보였다. 이에 반해 MMPI III를 처리한 세포는 약물 농도와 반응시간에 따른 세포자멸사 및 세포괴사에서 차이를 보이지 않았다. 두 약물을 동시에 투여한 군은 그렇지 않은 군에 비해 세포 자멸사 및 세포괴사에서 차이가 없었다. Doxorubicin에 Fas 중화항체를 추가한 군과 doxorubicin 단독 투여 군 사이에 세포자멸사 및 세포괴사에서 차이가 없었다. 결론: Doxorubicin과 같이 MMP 억제제를 골육종 치료에 사용하려면 wild-type p53 뿐만 아니라 wild-type p14를 가지고 있는 골육종 세포에 대한 검사가 필요할 것으로 사료된다. Purpose: The purpose of this study was to compare the proapoptotic effects of matrix metal-loproteinase inhibitor (MMPI) and doxorubicin on wild-type p53 osteosarcoma cell line, socalled U2OS cell line. Materials and Methods: U2OS cells were treated with MMP inhibitor III (MMPI III) and doxorubicin, either respectively or simultaneously. In cells treated with doxorubicin, Fas-neutralizing antibody so called ZB4 was additionally treated to examine whether the doxorubicin played a role through the Fas/FasL pathway. Cells were analysed regarding to apoptosis and cell death by flow cytometry. Results: U2OS cells incubated with doxorubicin showed significant amount of cell death in dose-dependent manner. However, those incubated with MMPI III mostly remained viable state. In addition, there is no relationship between two drugs. Cells treated with doxorubicin and ZB4 at the same time did not show down regulation of apoptosis through inhibition of Fas/FasL pathway. Conclusion: It is important to re-examine MMP inhibitor's effect on other osteosarcoma cell line with wild-type p14 as well as wild-type p53 to evaluate its proapoptotic effect.
난소의 성끈 종양을 닮은 자궁종양 -세포학적 소견 1예 보고-
김인선,한은미,정운용,이주한,염범우,Kim, In-Sun,Han, Eun-Mee,Jung, Woon-Yong,Lee, Ju-Han,Yeom, Bum-Woo The Korean Society for Cytopathology 2003 대한세포병리학회지 Vol.14 No.2
Uterine stromal tumors with features of ovarian sex-cord differentiation are relatively rare. The neoplasms composed of sex cord-like components in more than 50% of the tumor are classified as group II. We report the cytologic findings of a case of uterine tumor resembling ovarian sex-cord tumor. The cervical smears of a 62-year-old woman with submucosal tumor showed loose aggregates of spindle cells as well as glandular or tubular structures of round cells with a distinct ceil membrane and a prominent small nucleolus. Because uterine stromal tumor can have sex cord differentiation, its possibility should be considered in the interpretation of cervical smears.
羅泳燦,廉範愚,金世民 고려대학교 의과대학 1984 고려대 의대 잡지 Vol.21 No.1
To evaluate the binding reactions of various phytohemagglutinins (PHAs) on the gastric carcinoma cells, the author had undertaken an experiment using purified PHAs, such as Concanavalin A(Con-A), wheat germ agglutinin(WGA), soybean agglutinin(SBA), Dolichos biflorus agglutinin(DBA), Ulex europaeus agglutinin I (UEA I), peanut agglutinin(PNA), Ricinus communis agglutinin I (RCA I), and Pinellia ternata agglutinin(PTA). The tissue samples used were gastric tissue from subtotal gastrectomy or fiberoptic biopsy, diagnosed by pathologists as well differentiated adenocarcinoma and poorly differentiated adenocarcinoma signet-ring cell type adenocarcinoma, and dysplasia. The results obtained are as follows; 1. On the well-differentiated adenocarcinoma, all the PHAs except UEA I showed cytoplasmic granular reactions of the tumor cells and linear reactions along the intraluminal borders of the tumor glands. 2. On the poorly differentiated adenocarcinoma, RCA I and UEA I showed cytoplasmic reactions, and other PHAs showed variable reactions from positive to focal positive. 3. In signet-ring cell carcinoma, PNA, SBA, RCA I and UEA I showed weak reactions in the cytoplasm. 4. On the dysplastic cells, Con-A, WGA, PNA, RCA I and PTA showed cytoplasmic reactions and SBA, UEA I and DBA showed no reaction. With the above results, the binding of PHAs on the tumor cells of the stomach reveled variable patterns, depending on the degree of differentiation and the kinds of PHAs used.
타액선 암종세포의 식물응집소 수용체 분포에 관한 면역조직화학적 연구
채양석,염범우,이대일 고려대학교 의과대학 1987 고려대 의대 잡지 Vol.24 No.1
Malignant tumors of the salivary glands are variable in their histologic pictures, and their histochemical changes in membrane carbohydrate composition have not been fully understood. The author selected formalin fixed, paraffin embedded normal tissue and malignant tumors of salivary gland, and performed immunohistochemical study on the selected tissue materials by avidin biotin peroxidase complex method using lectins such as Concanavalin A (Con A), Soybean agglutinin (SBA), Wheat germ agglutinin (WGA), Dolichos biflorus agglutinin (DBA), Ulex europaeus agglutinin I (UEA I), Peanut agglutinin (PNA), Ricinus communis agglutinin I (RCA I), and Pinellia ternata agglutinin (PTA). The results are summarized as follows: 1. Lectins showing different reaction pattern between normal and malignant tumor cells of the salivary glands were DBA, UEA-1, SBA and PNA. 2. DBA and SBA showed positive reaction with normal duct epithelium, but they did'nt react with malignant turner cells. 3. UEA-1 revealed positive reaction with normal duct epithelium and cancer cells of the mucopeidermoid carcinoma. But it did'nt react with adenoid cystic carcinoma cell and acinic cell carcinoma cell. 4. PNA showed positive reaction with mucoepidermoid carcinoma cell and negative reaction with nor-mal duct epithelium, adenoid cystic carcinoma and acinic cell carcinoma cell. Above findings suggest loss of N-acetyl-galactosaminyl moiety in malignant tumors of salivary gland, loss of L-fucosyl in adeniod cystic cacinoma and acinic cell carcinoma, which are present on normal duct epithelium, and appearance ofβ-D-galactosyl moiety in mucoepidermoid carcinoma which is absent on normal duct epithelium.