환경호르몬의 영향과 규제방안에 관한 연구

여민경(Yeo Min-Kyeong) 한국형사법무정책연구원 2002 형사정책연구원 연구총서 Vol.- No.-

수열합성법으로 제조된 Zn-TiO₂ 나노입자와 TiO₂ 나노입자가 zebrafish 배발생에 미치는 영향

여민경(Min-Kyeong Yeo),김효은(Hyo-Eun Kim) 환경독성보건학회 2009 환경독성보건학회지 Vol.24 No.4

In this study, we investigated the biological toxicity of nano-scale Zn (0.1, 0.5, and 1 ㏖%)-doped TiO₂ and pure TiO₂ nanoparticles using zebrafish embryogenesis as our model organism. Zn-doped TiO2 nanoparticles were prepared using a conventional hydrothermal method for the insertion of zinc into the TiO₂ framework. The characters of Zn-doped TiO₂ (0.1%, 0.5%, 1%Zn) and pure TiO₂ were about 7~8 ㎚. These sizes were smaller than 100~200 ㎚ of TiO₂ was prepared using the sol-gel method. Particularly, in this study, we found no significant biological toxicity in the hatching rate and abnormal rate under expose pureTiO₂ and Zn-doped TiO₂ nanoparticles were prepared using a conventional hydrothermal method of zebrafish. It was different from the biological damage under TiO₂ nanoparticles were prepared using sol-gel method. We assessed that the damage was not linked to the particle’s nanometer size, but rather due to the prepare method. Moreover, TiO₂ nanoparticles were prepared using a hydrothermal method were not shown to cause cytotoxic effects, like apoptosis and necrosis, that are the major markers of toxicity in organisms exposed to nanomaterials. Therefore, there is some relationship with biological toxicity of nanoparticles and the prepare method of nanometer size particles.

The Influence of Bisphenol A on Selenoprotein N Expression Genes during Zebrafish Embryogenesis

Young-Gook Gwak(곽영국),Min-Kyeong Yeo(여민경) 환경독성보건학회 2008 환경독성보건학회지 Vol.23 No.3

내분비계 장애물질 중 에스트로겐성 특성을 지닌 것으로 알려진 bisphenol A에 폭로되었을 때에 나타나는 이상 증상과 selenoprotein N 결핍 증상은 유사성이 있는 것으로 관찰되었다. 이에 본 연구에서는 bisphenol A에 폭로된 생물체의 selenoprotein N 유전자 발현에 영향을 미치는지에 관해 연구 하였다. 실험동물은 zebrafish (Danio rerio, wild type)의 수정란을 사용하였다. Zebrafish의 수정란은 서로 다른 농도의 bisphenol A (0.1, 1, 10 ppm)에 노출하였다. 각각의 폭로 환경에서 부화된 치어를 시료로 selenoprotein N 유전자 발현을 RT-PCR 방법에 의해 알아보았다. 그 결과, bisphenol A는 척추이상과 심장이상이 나타나 selenoprotein N 이상시 나타나는 현상과 유사성을 보였다. Selenoprotein N 유전자 발현은 bisphenol A 폭로 농도가 높아짐에 따라 유전자 발현 농도가 낮아지는 것으로 관찰되었다. 특히 1 ppm 농도에서는 대조군이나 0.1 ppm 농도보다 catalase의 활성이 높게 나타나 특정 농도에서 bisphenol A에 의한 영향이 나타나는 것을 알 수 있었다.

Environmental fate and bioaccumulation of nano TiO₂ in paddy microcosm

JungIn Kim(김정인),Hyung-Geun Park(박형근),Min-Kyeong Yeo(여민경) 환경독성보건학회 2014 한국독성학회 심포지움 및 학술발표회 Vol.2014 No.11

Somite stage에 노출된 은나노 입자가 zebrafish 발생에 미치는 영향

홍석호(Suk ho Hong),송형귀(Hyung Gwi Song),정경준(Kyeong-jun Jeong),고광일(Kwang Il Ko),여민경(Min-Kyeong Yeo) 환경독성보건학회 2009 환경독성보건학회지 Vol.24 No.1

Nanotechnology, one of the technologies that forms the core of the recent scientific innovation. is used much in our real lives. Especially products that use nano silver are being sold, with its positive characteristics resulting from the antibacterial effects of both nano materials and silver. But critiques have pointed out that nano silver diffused into everyday life too quickly as we do not have done any comprehensive research about the material, and worry that nano silver will affect the ecology adversely. Therefore, this research focuses on investigating the toxicity of silver nanoparticles first. To compare the effects of exposure to silver nanoparticles at pre-somite stage and somite stage (10 hours after fertilization), we exposed zebrafish embryos to silver nanoparticles(15, 30 ppt) during embryogenesis, and then checked the details of catalase enzyme activity. The hatch rate decreased in the silver nanoparticles exposed groups (15 and 30 ppt); furthermore, the hatched fishes had an abnormal notochord, damaged eyes and curved tail. The catalase activities of the 15 ppt exposed group at somite stage increased relative to those in the control group. Therefore, the silver nanoparticles could seriously damage the development of zebrafish embryos. Especially, exposure to silver nanoparticles at somite stage did severer damage than exposure since pre-somite stage did.

유,무기성 악취제거용 미생물 포괄고정담체 제작을 위한 PEGDA 및 TMEDA 최적 주입조건 도출[2]

김선진 ( Sun Jin Kim ),이명주 ( Myoung Joo Lee ),김태형 ( Tae Hyeong Kim ),김선응 ( Seon Ung Kim ),여민경 ( Min Kyeong Yeo ),황선진 ( Sun Jin Hwang ) 한국물환경학회 2010 공동 추계학술발표회 Vol.2010 No.-

해파리의 특성을 활용한 하수처리장 처리수 내 미량오염물질 제거 기술

박혁진 ( Hyeok Jin Park ),김은진 ( Eun Jin Kim ),추경실 ( Kyung Sil Choo ),심주은 ( Joo Eun Shim ),여민경 ( Min-kyeong Yeo ) 한국공업화학회 2024 공업화학 Vol.35 No.1

본 연구에서는 자포동물인 보름달물해파리의 면역반응을 유도하여 추출한 점액물질(jellyfish extract at immunity reaction, JEI)을 활용하여 하수처리장 처리수 내 잔존하는 미량오염물질(중금속과 미세플라스틱)의 제거 가능성에 대해 평가하였다. 실험은 자-테스터(jar-tester)를 이용한 lab 규모와 자체 개발한 관접속형 혼화반응 시스템과 농축일체형 분리장치를 연계한 pilot system 규모의 두 방법으로 수행하였다. 모든 실험 조건에서 JEI는 기존 음이온 고분자에 비해 미량오염물질 제거효율이 유사하거나 높은 것으로 분석되었다. 특히 pilot system 규모로 JEI를 하수처리장의 방류수와 혼합하여 사용하면 두 번의 혼합과정에 의해 JEI와 미량오염물질의 결합이 극대화되고 이로 인해 미량오염물질 제거율이 매우 향상됨을 확인하였다. 이러한 실험 결과를 토대로 본 기술을 기존의 방식에서 벗어나 생태계 위해성이 적고 무기성 폐기물 발생을 최소화할 수 있는 미량오염물질 제거 방법으로 제안하는 바이다. The present study was aimed to evaluate the removal of the trace pollutants (heavy metals and microplastics) in the sewage treatment plant by using the jellyfish Extract at Immunity reaction (JEI) of Aurelia coerulea. The experiment was conducted on two different scales: the lab scale using a Jar-tester and the Pilot system scale equipped with two newly developed devices in the laboratory, the active tube connection mixed system and the concentration integrated separation device. Compared to anionic polymers currently used in the field, JEI showed similar or higher efficiency to remove the trace pollutants. When JEI was added to the effluent through the Pilot system, the combination of JEI and the trace pollutants was maximized through two mixing processes, and as a result, the removal rate of the trace pollutants was greatly improved. Based on these results, we propose the present technology as an alternative to removing trace pollutants that can reduce ecosystem risk and minimize the generation of inorganic waste, away from the existing method.

에스트로겐성 환경물질이 zebrafish의 배발생 단계에 미치는 영향

여민경 경희대학교 2004 環境硏究 論文集 Vol.13 No.-

The effects of bisphenol A and nonylphenol on the embryogenesis and the hatching rate of zebrafish embryo were investigated. also, tee changer of tee catalyse activity's for A week, A week, and 4 week port hatching fry's exposed to bisphenol A(1000ng/1) and nonylphenol(1000ng/1) during embryogenesis were investigated. Development stage ways rapid in tee group exposed to bisphenol A of 1000ng/l at 10.75 hpf(hour port- fertilized) than the groups exposed during early embryogenesis period. Stacking rate war increased in the group opposed to nonylphenol than control, but lower for 8.75 hpf compared with tee other groups. Catalyse activites for zebrafish fries exposed to bisphenol A of 1000ng/l during 1 week post-hatching were significantly lower, compared to tee control. catalyse activities for 2 week, 4 week post hatching zebrafish fries exposed to nonylphenol(1000ng/1) during embryogenesis were significantly increased, compared to tee control. In conclusion, the damages of an endocrine disrupter were highest in the group exposed a the earlier development stage. And the damage have on effect on embryogenesis and growth period.

그람 음성균에 미치는 TiO₂ 광살균 효과

여민경 경희대학교 2006 環境硏究 論文集 Vol.15 No.-

In order to investigate the effect of TiO₂ photo killing on gram negative bacteria during UV-A light illumination. The survival of E. Cold bacteria were counted as a function of photo-illumination time. During the photoreaction, there was the effect of photo killing on E. Cool. Also, catalyse activity was changed at that time. These results suggest that the photo killing reaction is cause by a partial decomposition of the outer membrane. It seems like the toxic of other living things include human. Because it produced free radical during the TiO₂ photoreaction. It is need to use safely the TiO₂ coating method unattached to living things.

수용액에 용출된 에폭시수지 화합물의 TiO₂광분해효과와 생물독성에 미치는 영향

여민경,조은정 한국환경독성학회 2004 환경독성보건학회지 Vol.19 No.3

Epoxy resins are mostly used as a molding material for drinking water tank Bisphenol A is used as a constituent material for epoxy resins and is widely suspected to act as an endocrine disruptor In this study, we investigated embryo hatching in zebrafish reared to water undergone leaching process of expoxy resin and found a decreased survival rate Bisphenol A eluted from epoxy resin to drinking water tank was completely degraded by T₁O₂, photocatalysis We detected 7 8 ng/ml of bisphenol A in epoxy resin tank and observed that the concentration was undetectable after 48h photocatalysis over T₁O₂ There was no toxicity in hatching rates in zebrafish and morphugenesis after photocatalysis The effect of T₁O₂, photocatalysis reactions on the catalase activities to the fly stage of zebrafish was also examined At 1 week post hatching, catalase activities were higher both in the group of epoxy resin with 48 h T₁O₂ photocatalysis and to the T₁O₂ photocatalysis for 48 hours were higher than control group However catalase activities of the treatment group of epoxy resin by T₁O₂ photocatalysis for 48 hours were similar to control to 5 weeks post hatching tries In conclusion, the toxicity of T₁O₂ photocatalysis was not observed to this zebrafish.