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저온 프린팅 공정을 통한 콜라겐/실크-피브로인/세포외기질이 포함된 조직재생용 세포담체 개발
허준태(JunTae Huh),이형진(Hyeongjin Lee),여명구(MyungGu Yeo),김용복(YongBok Kim),양지훈(Gi-Hoon Yang),구영원(YoungWon Koo),여미지(Miji Yeo),임정형(JungHyung Lim),김근형(GeunHyung Kim) 대한기계학회 2016 대한기계학회 춘추학술대회 Vol.2016 No.12
In tissue engineering, biomaterials must be biocompatible, biodegradable, and mechanically stable to be used for biomedical scaffold. Among various biomaterials, natural polymers are widely used for great biocompatibility. However, due to their low mechanical properties, it has been a challenge to build desired 2D/3D structure using natural polymers. To overcome this problem, in this study, we used low temperature 3D printing to create a scaffold with collagen/silk-fibroin(SF)/extracellular matrix(ECM). Then, fibroblast cells were grown on the fabricated scaffold. Various in vitro cellular activities (cell-viability, MTT assay) for pure collagen, pure SF, collagen/SF, and collagen/SF/ECM scaffolds were compared. The collagen/SF/ECM scaffold showed 30% higher cell metabolic activities than pure collagen scaffold.
플라즈마 애칭을 이용한 나노 표면 구조를 가지는 마이크로 파이버 제작
전호준(HoJun Jeon),안승현(SeungHyun Ahn),이형진(Hyeongjin Lee),여명구(MyungGu Yeo),김용복(YongBok Kim),김민성(Min Seong Kim),이경호(KyoungHo Lee),김근형(GeunHyung Kim) 대한기계학회 2014 대한기계학회 춘추학술대회 Vol.2014 No.11
In a recent study, a hierarchical structure of coexistent nano- and microstructures induced high cellular activities, such as proliferation and differentiation. In this study, an easy and versatile method of generating a nano-sized patterned surface on a nonwoven mat of electrospun poly(e-caprolactone) (PCL) fibers is described. A hierarchical structured pattern could be achieved on the surface of the PCL fiber coated with collagen solution using a modified plasma-treatment method with nano-sized template (800 nm). This new type of plasma-treated PCL fiber mat has high potential as a biomaterial, and can be employed for various tissue engineering applications.
에어로졸 세포 프린팅 기법을 이용한 세포가 포함된 콜라겐/알지네이트 세포담체 개발
이형진(Hyeongjin Lee),김용복(YongBok Kim),안승현(SeungHyun Ahn),전호준(HoJun Jeon),여명구(MyungGu Yeo),김민성(Minseong Kim),이경호(KyoungHo Lee),김근형(GeunHyung Kim) 대한기계학회 2014 대한기계학회 춘추학술대회 Vol.2014 No.11
A modified dispensing method for fabricating three-dimensional (3D) cell-laden alginate/collagen scaffolds is presented that utilizes aerosolized calcium chloride solutions for crosslinking. To evaluate the process, preosteoblasts (MC3T3-E1) mixed with 3.5 wt% alginate and 2 wt% collagen(pH 7.5) were fabricated into a 3D scaffold (15 x 15 x 1.2 mm3). The fabricated cell-laden structure was highly porous and had uniformly designed pore size and shape. The scaffold-embedded cells were 87% viable compared to the initial cell viability, and the cell distribution was homogeneous. This innovative cell dispensing technique is likely to be a promising fabrication tool for obtaining functional 3D scaffolds for tissue regeneration.
가교 공정을 통한 세포 함유 알지네이트 세포담채 제작 기법
안승현(SeungHyun Ahn),전호준(HoJun Jeon),이형진(Hyeongjin Lee),김용복(YongBok Kim),여명구(MyungGu Yeo),김민성(Minseong Kim),이경호(KyoungHo Lee),김근형(GeunHyung Kim) 대한기계학회 2014 대한기계학회 춘추학술대회 Vol.2014 No.11
In this study, we propose a new cell-laden process consisting of a dispensing method and a new cross-linking method. A new cell-printed scaffold consisting of cell-embedded alginate struts is designed. The alginate struts are stacked in an interdigitated pattern in successive layers to acquire a three-dimensional (3D) shape. To show the feasibility of the method, we used osteoblasts-like cells. Based on these optimized process conditions, we successfully fabricated a threedimensional, pore structured, cell-laden alginate scaffold. During long cell culture periods, the osteoblasts-like cells in the alginate scaffold survived and proliferated well.
골 조직 재생을 위한 전기수력학적 공정을 이용한 섬유구조의 PCL/ceramic 세포담체 제작
김민성(Minseong Kim),이형진(Hyeongjin Lee),전호준(HoJun Jeon),여명구(MyungGu Yeo),김용복(YongBok Kim),양지훈(Gi-Hoon Yang),김원진(WonJin Kim),김근형(GeunHyung Kim) 대한기계학회 2016 대한기계학회 춘추학술대회 Vol.2016 No.12
In this study, we fabricated a new ceramic fibrous scaffold, using the initial jet of an electrospinning process and ethanol media as a target. The fabricated three-dimensional (3D) fibrous ceramic structure was configured with multilayered micro-sized struts consisting of randomly entangled micro/nanofibrous architecture, similar to that of native extracellular matrixes (ECMs). The fabrication of the ceramic structure was highly dependent on various processing parameters, such as the surface tension of the target media, and the flow rate and weight fraction of the polymer solution. As a tissue regenerative material, the 3D fibrous ceramic scaffold was cultured with pre-osteoblasts to observe the initial cellular activities in comparison with a solid freeform fabricated 3D scaffold sharing a similar structural geometry. The micro/nanofibrous 3D fibrous ceramic strut scaffold exhibited significantly high initial cell attachment, proliferation, and viability compared to solid freeform fabricated 3D scaffold.
폴리카프로락톤/알지네이트로 구성된 바이오 용액코팅 공정의 하이브리드 세포담체 제작
김용복(YongBok Kim),이형진(Hyeongjin Lee),안승현(SeungHyun Ahn,),전호준(HoJun Jeon),여명구(MyungGu Yeo),이경호(KyoungHo Lee),김민성(Minseong Kim),김근형(GeunHyung Kim) 대한기계학회 2014 대한기계학회 춘추학술대회 Vol.2014 No.11
Alginate has been widely used in various tissue regenerations, and it has also recently been applied to bone tissue regeneration due to its ability to promote cell growth and bone tissue development. However, although alginate has excellent biocompatibility for the tissue regeneration, it suffers from low mechanical properties and low processability to obtain artificially controlled internal microstructures. In this study, to overcome the limitations, we propose a new cell-coating process supplemented with cells in alginate. The cell-coated PCL/alginate scaffold consisted of alginate with a mixture of cells coated on the PCL struts. The fabricated scaffold showed highly cellular behavior and uniform cell density.