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솜양지꽃(Potentilla discolor Bunge)의 초저온동결보존을 위한 최적 조건 탐색
양우형 ( Woo Hyeong Yang ),용성현 ( Seong Hyeon Yong ),박동진 ( Dongjin Park ),설유원 ( Yuwon Seol ),최은지 ( Eunji Choi ),정미진 ( Mi Jin Jeong ),최명석 ( Myung Suk Choi ) 한국산림과학회(구 한국임학회) 2018 한국산림과학회지 Vol.107 No.3
본 연구는 희귀자생식물인 솜양지꽃의 효율적인 초저온 보존 조건을 탐색하고자 행하였다. 종자의 활력은 PVS2와 PVS3 용액 처리구에서는 약 80% 이상으로 대조구보다 훨씬 높은 활력을 보였다. 종자의 활력은 PVS3가 PVS2 처리보다 높게 나타났다. 종자의 활력은 sucrose처리에도 불구하고 대조구보다 낮았다. PVS2의 60분 처리구와 PVS3의 30분 처리구에서 95%의 발아율을 보였으나 그 외 처리구에서는 발아율이 낮았다. PVS2와 PVS3 처리구에서 배양된 유묘의 생장은 PVS2와 PVS3 용액 30분 처리구를 제외하고는 대조구에 비해 생장이 좋지 않았다. 두 가지 초저온동결보존법 간 유묘 생장에서는 encapsulation법과 vitrification법을 비교한 결과 미세한 차이를 보였으나 통계적으로는 유의성이 없었다. 본 연구는 솜양지꽃의 보존에 도움이 될 것으로 판단된다. This study was conducted to investigate the effective cryopreservation condition of Potentilla discolor Bunge, a rare native plant. Seed viability was more than 80% in PVS2 and PVS3 solution treatments. Seed viability was higher in PVS3 than PVS2 treatment. Seed viability was lower than control in spite of sucrose pretreatment. The germination rate was 95% at 60 min of PVS2 treatment and 30 min of PVS3 treatment but the germination rate was low at other treatments. The growth of the seedling wasn't doing that of the control except for treat PVS2 and PVS3 solution for 30 min. There was no statistically significant difference between the encapsulation method and the vitrification method in the seedling growth between the two cryogenic storage methods. This study is expected to be applied to future conservation methods of Potentilla discolor.
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Changes in the Physicochemical Characteristics of Sancho Oil according to the Purification Process
강승미,김학곤,양우형,용성현,박동진,박준호,최명석,Kang, Seung Mi,Kim, Hak Gon,Yang, Woo Hyeong,Yong, Seong Hyeon,Park, Dong Jin,Park, Jun Ho,Enukwa, Ettagbor Hans,Choi, Myung Suk The Korean Society of Medicinal Crop Science 2017 한국약용작물학회지 Vol.25 No.5
Background: Sancho oil extracted from Zanthoxylum schinifolium (Siebold & Zucc) is a useful edible oil that has been in use for a long time, but it is known to be susceptible to rancidity. Sancho oil purification can remove impurities to prevent rancidity. This study was performed in order to improve the quality of sancho oil and enhance its availability throughout the purification process. Methods and Results: Sancho oil extracted in Hadong, Korea was refined via the degumming and deoxygenation processes, following which we examined the changes in the polyphenol content, fatty acid content and antioxidant activity of the oil. Acetic acid was effective for deoxygenation of sancho oil and 2 N NaOH was effective for its deoxidation. The polyphenol content and antioxidant activity were reduced by the purification process. Saturated fatty acids contents did not vary with the degumming and deoxygenation processes, however the content of unsaturated fatty acids were slightly reduced. Conclusions: This study suggests that the process of sancho oil purification used in this study will contribute to the increased use and storage of sancho oil.
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노거수 및 보호수 왕버들 삽목증식에 미치는 상토의 영향
송현진 ( Hyun Jin Song ),정미진 ( Mi Jin Jeong ),김학곤 ( Hak Gon Kim ),서영롱 ( Young Rong Seo ),임현정 ( Hyeon Jeong Im ),양우형 ( Yang Woo Hyeong ),박동진 ( Park Dong Jin ),윤석락 ( Yun Seok Lak ),마호섭 ( Ho Seop Ma ),최명석 ( 한국산림과학회 2015 한국산림과학회지 Vol.104 No.1
Old growth and protected-trees are worth history and culturally in Korea. This research carried out to investigate habitat characterization and the vegetative propagation methods of Salix chaenomeloides(100-600 years old trees) in Gyeongsangnamdo Province. It has been preserved in good and grow naturally in area surrounding village(4 trees), riverside(1 tree) and field(1 tree). Cutting from old growth and protected trees was conducted using 10 kinds of bedsoils. It was survived above 93% in all bedsoils. Growth of cuttings is a distinct difference according to bedsoils and tree ages. Growth of cutting was high on CBS, followed mixed bedsoils (VPMP) including vermiculite, peatmoss and perlite. Root collar diameter was difference depend on tree ages and bedsoils. All trees are produced 1 to 4 branch. Root growth of cutting was 20 cm on CBS and VPMP, which is the best shoot growing on cutting. Bedsoil with physico-chemical characteristics of high moisturizing ability, high air permeability, high EC value and slightly acid may be suitable for efficiently cutting propagation of old growth and protected S. chaenomeloides. CBS and VPMP bedsoil are favorable for propagating this species by cuttings.
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수분결핍에 따른 식물의 생리적 변화와 내건성 자생식물종 선발에 관한 연구
임현정 ( Hyeon Jeong Im ),송현진 ( Hyun Jin Song ),김학곤 ( Hak Gon Kim ),서영롱 ( Yeong Rong Seo ),조호준 ( Ho Jun Cho ),양우형 ( Woo Hyeong Yang ),정미진 ( Mi Jin Jeong ),마호섭 ( Ho Seop Ma ),최명석 ( Myung Suk Choi ) 한국임학회 2014 산림과학 공동학술대회 논문집 Vol.2014 No.-
식물은 건조 등의 스트레스 하에 놓여 있고, 수분을 효율적으로 이용하는 내건성 식물의 선발은 biomass 생산과 종보존을 위해 매우 중요하다. 본 연구는 식물의 내건성 생리적지표를 이용하여 내건성 식물을 선발하였다. 자생식물 110종 중 발아율이 높은 수종인 26종을 공시수종으로 선발했다. 내건성을 조사하기 위해 균일한 식물체에 무관수 처리하여 고사 소요일을 조사하였다. 식물의 수분스트레스에 비례하여 증가하는 프롤린 함량을 분석하여 내건성 평가지표로 활용하였다. 수분결핍에 따른 고사소요일을 조사한 결과, 2일째부터 고사하기 시작하여 10일 이후에도 생존하였다. 그 결과 새, 도깨비가지, 긴담배풀, 매듭풀, 비수리, 참느릅나무는 내건성 수종으로 입증되었다. Proline 분석 결과, proline 함량은 선발된 내건성 식물은 건조조건 부여 전과 내건성 민감성 수종보다 높았다. 본 연구는 내건성 식물종의 육종에 크게 기여할 것으로 판단된다. Many plants are stressed by prolonged periods of dry weather. Selecting trees that use water efficiently must be important in a future plant biomass and conservation. This study was selected of drought tolerance plant by using physiological indicators for drought tolerance. 110 plants germinated and highly germinating 26 species were selected. Four weeks old plants were stopped to irrigation to find out withering day by moisture deficiency. The proline content of the water stressed leaf tissues was analyzed and withering day according to moisture deficiency was examined. When stopped to irrigation, whithering day of plant species was variable from 2 to 10 days. Drought resistance plant confirmed 6 species as Ulmus parvifolia, Lespedeza cuneata, Kummerowia striata, Carpesium divaricatum, Solanum carolinense, and Arundinella hirta Proline content in selected drought tolerance plant was significantly increased than before stopped irrigation and drought susceptible plants. These results can be served breeding of drought tolerance plant from Korean native plants.
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김학곤 ( Hak Gon Kim ),송현진 ( Hyun Jin Song ),서영롱 ( Young Long Seo ),임현정 ( Hyun Jeong Im ),조호준 ( Ho Jun Jo ),양우형 ( Woo Hyeong Yang ),양재경 ( Jae Kyung Yang ),최명석 ( Myung Suk Choi ) 한국임학회 2014 산림과학 공동학술대회 논문집 Vol.2014 No.-
국내 바이오매스의 활용도와 효율성을 높이기 위해 산림청 조림권장수종을 포함한 86종의 산림자원에 대하여 화학적 성분을 조사하였다. 각 수종은 NREL의 분석방법으로 저분자화합물, lignin, hemicellulose, cellulose의 함량을 조사하였다. 또한 이들 화학적 조성성분간의 상관관계에 대하여 SPSS 대응표본 T검정을 통하여 조사하였다. 그 결과 국내 자생 목본 수종의 화학적 성분은 매우 큰 차이를 보였다. Extractives의 함량이 가장 높았던 종은 가막살나무였고, lignin의 함량이 가장 높았던 종은 명자나무였다. Hemicellulose 함량이 가장 높았던 종은 서양측백 이였고, 낮은 종은 단풍나무였다. Cellulose의 함량이 가장 높았던 종은 벚나무였다. Lignin의 함량이 높을수록 헤미셀룰로오스와 셀룰로오스의 함량은 감소하는 경향을 보였다. 본 연구 결과는 조림권장수종에 대한 이용성 증대에 기여할 것으로 판단된다. In order to increase the efficiency and utilization of domestically producible biomass, wood chemical component for 86 woody plants was analyzed. Each tree species were collected, mecerated and then quantifiable the content of extractives, lignin, hemicellulose, cellulose using the method of NREL. Further, it was statistical analysed through SPSS corresponding sample T-test for correlation between chemical compositions. As a result, the chemical composition of domestic woody species showed a huge variable according to plant species and chemical component. Among woody plants, extractive content was highest in Viburnum dilatatum and lignin content was high contained in c haenomeles lagenaria. The hemicellulose content was highly contained in Thuja occidentalis, however a cer palmatum was lowest compared to other species. Cellulose was highly contained in Prunus serrulata. Statistically, we have found that the higher lignin content is, the more it cellulose content. These results will do much to alleviate of utilization of Korean native woody plants.
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