고려인삼과 미국삼의 종간잡종으로부터 재분화된 식물체의 특성

안인옥,이성식,이장호,이범수,인준교,양덕춘,Ahn, In-Ok,Lee, Sung-Sik,Lee, Jang-Ho,Lee, Bum-Soo,In, Jun-Gyo,Yang, Deok-Chun 한국식물생명공학회 2006 식물생명공학회지 Vol.33 No.1

교잡 1세대의 화서조직에서 재분화된 F1 유식물체는 고려인삼 유식물체에 비하여 지상부와 지하부 생육이 모두 양호하였으며, 줄기 색도 고려인삼 재분화 식물체의 줄기에 비하여 자색을 강하게 띄었으며 잎의 색도 진한 녹색을 나타내었다. 천풍, 연풍, 선원 등의 고려인삼의 품종 내에서는 Internal Transcribed Spacer (ITS)영역의 DNA PCR 패턴간에 차이점이 나타나지 않았으나, 고려인삼과 미국인삼은 각기 다른 PCR 패턴을 보였으며, 고려인삼과 미국인삼간의 교잡 1세대는 고려인삼과 미국인삼에 나타나는 고유한 PCR패턴을 모두 나타내었다. 교잡 1세대에서 유기한 캘러스와 재분화식물체는 조직배양 모본인 교잡 1세대와 동일한 PCR 패턴을 보임에 따라 교잡 1세대의 조직배양체는 ribosomal DNA의 ITS영역에서 유전적인 안정성을 나타내는 것으로 확인되었다. The characteristics of plantlets redifferentiated from calli of F1 hybrid between Panax ginseng and Panax quinquefolius were investigated. Growth of plantlets redifferentiated from F1 hybrid was superior to the plants redifferentiated from Korean ginseng. Stem color of plantlets redifferentiated from F1 hybrid was more purple than that from Korean ginseng and leaf color of the former was also greener than that of the latter. Chunpoong, Yunpoong and Seonweon which are belonged to Korean ginseng showed same PCR band(A), while American ginseng showed different PCR band (B) in Internal Transcribed Spacer (ITS) region. F1 hybrid exhibited both A and B PCR band which belonged to Korean ginseng and American ginseng, respectively. F1 hybrid calli and plantlets redifferentiated from F1 hybrid calli showed same PCR band with that of F1 hybrid plant in ITS region. Therefore it was confirmed that piantlets redifferentiated from F1 hybrid exhibited genetic stability in ITS region.

고려인삼 신품종 특성

이성식(Sung-Sik Lee),이장호(Jang-Ho Lee),안인옥(In-Ok Ahn) 고려인삼학회 2005 고려인삼학회 학술대회 Vol.- No.-

This paper reports the characteristics of 8 new cultivars for selected from Korean ginseng.<br/> The occurance of multi stems were the highest in Yunpoong(45%) and the lowest in Gumpoong(7%), but growth of aerial parts were the highest in Gumpoong and the lowest in Yunpoong among new cultivars. The ratio of seeds harvest were the highest in Gumpoong(85.4%) and the lowest in Chunpoong(69.1%), but number of seeds per plant were the highest in Yunpoong(108.3ea) and the lowest in Chunpoong(77.5ea) among new cultivars. The ratio of leaf burning were the highest in Chunpoong but the lowest in Yunpoong among new cultivars. In weight distribution of the different parts of the ginseng roots, the ratio of main root were high in Jakyungjong(63.1%) but low in new cultivars(49%-55.9%), but the ratio of lateral root were high in new cultivars(19.3-23.3%),<br/> but low in Jakyungjong(13.2%), the ratio of fine root were not different. Root yield declined in the order of Yunpoong, Gumpoong, Gopoong, Chunpoong, Sunpoong, Jakyungjong. The length of main root were the longest in Chunpoong(8.0㎝) but the shortest in Yunpoong(6.4㎝). The ratio of rusty-root was low in new cultivars(0.2-9.5%), but high in Jakyungjong(16.3%). <br/> The grade of red ginseng roots decreased in the order of Chunpoong, Gumpoong, Gopoong, Sunpoong, Yunpoong, Cheongsun, Jakyungjong. <br/> The total ginsenoside contents per dry weight in main roots was high in Gumpoong(8.53㎎), Yunpoong(8.13㎎), Gopoong(7.47㎎), but low in Chunpoong(5.73㎎), Sunpoong(4.87㎎).

인삼 배발생세포에서 재분화된 유식물체의 뿌리 발육에 미치는 Phytagel의 영향

안인옥(In-Ok Ahn),최영준(Young-Jun Choi),이성식(Sung-Sik Lee),이장호(Jang-Ho Lee),강제용(Jae-Yong Kang),유연현(Yun-Hyun Yu),정희돈(Hee-Don Chung) 고려인삼학회 2004 Journal of Ginseng Research Vol.28 No.4

This study was carried out to find out the optimum hardening condition for ginseng plantlets redifferentiated by tissue culture method. While a lot of root hair were observed on the root of seedling grown on the soil, few root hair were observed on the root of plantlet redifferentiated in vitro. On the medium solidified with 0.1 % phytagel, root hair was not observed and root weight, root width and laternal root development were also very poor. While raising the phytagel concentration on the media, root hair began to increase and root weight, root width and laternal root development were improved. Vascular tissue of plantlet grown on the medium with 0.1 % phytagel was very poor, but that of plantlet grown on the medium with 0.8% phytagel was very good.

Transition of Cellular Oxidation-Reduction Status in Accordance with Somatic Embryogenesis during the Cultures of Ginseng (Panax ginseng)

Ahn In Ok(安仁玉),Kim Byung Dong(金昞東) 한국원예학회 1992 Horticulture, Environment, and Biotechnology Vol.33 No.2

인삼 신품종의 뿌리부위별 진세노사이드 함량 및 패턴비교

안인옥(In-Ok Ahn),이성식(Sung-Sik Lee),이장호(Jang-Ho Lee),이미자(Mi-Ja Lee),조병구(Byung-Gu Jo) 고려인삼학회 2008 Journal of Ginseng Research Vol.32 No.1

인삼의 부위별 진세노사이드 패턴 유사성과 상관관계를 알아보고자 본 시험을 수행하였다. 진세노사이드 단위함량과 총함량은 고풍이 각각 18.9 ㎎/g, 596 ㎎/g으로 가장 높았고 연풍, 금풍, 선풍이 뒤를 이었으며, 천풍은 각각 8.0 ㎎/g, 209.5 ㎎/g으로 고풍의 절반에도 미치지 못하였다. 부위별로 보면 뇌두의 진세노사이드 단위함량과 총함량은 연풍이 가장 높았으며, 동체와 지근 및 세근에서는 고풍이 높았다. 뿌리와 각 부위의 진세노사이드 패턴 유사성은 지근과 뇌두가 각기 0.95, 0.94로 높았으며 동체와 세근은 각기 0.78, 0.80으로 다소 낮았다. 지근에서 품종별 진세노사이드 패턴 유사성을 보면 천풍, 연풍, 고풍, 금풍이 각기 0.98, 0.98, 0.96, 0.98로 아주 높았으며, 선풍은 0.87로 다소 낮았다. 뿌리와 각 부위의 진세노사이드 상관계수는 지근에서 0.843으로 가장 높았으며 동체, 세근, 뇌두 순으로 낮아졌다. 또한 단위함량과 총함량의 상관계수는 0.933으로 매우 높게 나타났다. This study was carried out to evaluate the basic information on ginsenoside contents and pattern similarity in five cultivars of Panax ginseng C.A. Meyer. Among five cultivars the unit content and total content of ginsenosides were the highest in Gopoong cultivar as 18.9 ㎎/g and 596 ㎎/root, respectively. The unit content and total content of ginsenosides decreased in the order of Yunpoong, Gumpoong, Seonpoong and Chunpoong cultivar. Ginsenoside pattern similarity between tap root and lateral root was high as 0.95 but that between tap root and fine root was low as 0.72. Correlation of ginsenoside contents between tap root and lateral root exhibited the highest value as 0.843 and decreased in the order of main root, fine root, and rhizome. And the correlation value between unit content and total content of ginsenoside was very high as 0.933.

Regeneration of Ginseng (Panax ginseng C.A. Meyer) Through the Maturation Process of Somatic Embryos

Ahn In Ok(안인옥) 한국원예학회 1996 Horticulture, Environment, and Biotechnology Vol.37 No.6

인삼 현탁배양세포의 생장과 페놀화합물의 생성에 미치는 옥신과 당의 영향

안춘희(Ahn Chun-Hee),윤화모(Yoon Wha-Mo),안인옥(Ahn In-Ok) 한국원예학회 1996 Horticulture, Environment, and Biotechnology Vol.37 No.2

인삼 품종별 종자의 크기가 개갑율 및 묘삼생육에 미치는 영향

이준수(Joon-Soo Lee),이성식(Sung-Sik Lee),이장호(Jang-Ho Lee),안인옥(In-Ok Ahn) 고려인삼학회 2008 Journal of Ginseng Research Vol.32 No.3

본 실험은 품종별 종자의 크기가 묘삼의 소질에 미치는 영향을 조사하고 우수한 묘삼생산의 기초자료로 삼고자 수행하였다. 품종간 종자크기별 개갑율과 생존율을 조사한 결과 개갑율은 품종에서 선운이 가장 우수하였고, 천풍의 개갑율이 가장 불량하였다. 종자크기는 개갑율 과 생존율에 영향을 미치지 못하였다. 경장, 경직경, 엽면적은 종자의 크기가 클수록(4.7 ㎜ 이상) 생육이 우수하였으며 종자의 크기가 작을수록 생육이 불량하여 지상부 생육에 종자의 크기가 영향을 미치는 것으로 확인되었다. 근장, 근직경, 근생체중은 종자의 크기가 큰 것(4.7 ㎜ 이상)이 생육이 우수하였으며, 종자의 크기가 작은 것은 생육이 불량하였다. 종자크기별 이용가능 및 불용묘삼을 조사한 결과 종자의 크기가 클 수 록 이용가능 묘삼의 수량과 본수 증가하는 경향이었다. 품종에서는 연풍, 금풍, 고풍이 지상하부의 생육이 우수하였으며, 천풍은 지상하부의 생육이 낮았다. 사용가능 및 우량묘삼을 생산하기 위하여 4 ㎜이상의 종자만을 파종하여야 할 것으로 생각된다. This study was carried out to obtained the basic information for seed coat dehiscence and seedling performance according to seed size of eight ginseng cultivars. Seed size was separated into four grade(upper 5.0, 5.0-4.7, 4.7- 4.0, and under 4.0㎜ in diameter). Seed coat dehiscence and survival ratio of seedling were not affected by seed size. Aerial parts growth of seedling such as stem length, stem diameter, and leaf area of seedling were larger seed(upper 5.0 ㎜) better than those of small seeds(4.7-4.0, and under 4.0 ㎜ in diameter). Also root part growths such as root diameter, and fresh weight were larger seed(upper 5.0 ㎜) higher than those of small seeds(4.7-4.0, and under 4.0 ㎜ in diameter). However, root length was not affected by seed size. Growth status of aerial and root parts of ‘Yunpoong’, ‘Gumpoong’, and ‘Gopoong’ were better than those of other cultivars in each grade of seed sizes. The growth of characteristics of ‘Chunpoong’ and ‘Chungsun’ were less than those of other cultivars.

약토 시용방법이 인삼 양직묘 뿌리 생육에 미치는 영향

이성식(Sung-Sik Lee),천성기(Seong-Ki Cheon),이장호(Jang-Ho Lee),안인옥(In-Ok Ahn),신성련(Seong-Lyon Shin),최광태(Kwang-Tae Choi),이갑수(Gab-Soo Lee),이형식(Hyung-Sik Lee),정재동(Jae-Dong Chung) 고려인삼학회 2004 Journal of Ginseng Research Vol.28 No.4

This experiment was conducted to determine economic application method of Yakto among 6 application method: amount of Yakto application 3 plots(70ℓ, 25.5ℓ, 13.3 ℓ/kan), and added side dressing(3ℓ/kan) each plot. The reduced Yakto application(25.5ℓ/kan) with side dressing(3ℓ/kan) showed similar root diameter, fresh weight of roots and yield of good seedlings to standard Yakto application(70ℓ/kan). And also this application method of Yakto gave similar content of Ca and Mg, but the lower content of N, P, K in roots to standard Yakto application(70ℓ/kan). These suggested that 25.5ℓ Yakto application added side dressing(3ℓ/kan) per kan might be economical application method of Yakto.

경흔적 수에 의한 수삼의 연근판별

이성식(Sung-Sik Lee),이장호(Jang-Ho Lee),안인옥(In-Ok Ahn),김영창(Young-Chang Kim),방경환(Kyong-Hwan Bang),현동윤(Dong-Yun Hyun) 고려인삼학회 2007 Journal of Ginseng Research Vol.31 No.3

본 시험은 경흔적수 측정법으로 수삼의 연근을 판별하기 위하여, 4, 5, 6년근의 경흔적수 발생실태를 조사하였다. 경흔적수를 이용한 연근판별법은 3년근 경흔적부터 식별가능하여 경흔적수에 2를 더하면 년근판별이 가능하였다. 년근별 경흔적수가 4년생에서 2개 발생율이 89.5%, 5년생에서 3개 발생율이 79.7% 6년생에서 4개 발생율이 46.3%로, 4, 5년근의 판별은 가능하였으나, 6년근 판별은 정확도가 낮아 보조자료로 활용함이 타당하였다. 경흔적을 이용한 연근 판별의 제한요인으로 뇌두파손과 다경발생임을 확인하였고, 뇌두파손율과 다경발생율은 년근이 증가함에 따라 증가되었다. This experiment was conducted to find identification of ginseng root's age using the number of stem vestiges in rhizome. The number of stem vestiges in rhizome is a useful key to confirm the age of ginseng root as follow : 4-year-old root has two, 5-year-old root has three, 6-year-old root has four. The distribution of stem vestiges in rhizome each year root are as follow : 2 stem vestiges in 4-year-old root is 89.5%, 3 stem vestiges in 5-year-old root is 79.7%, 4 stem vestiges in 6-year-old root is 46.3%. However, the limiting factors of identification of ginseng root's age using the number of stem vestiges in rhizome is appearance of multi-stem per plant and appearance of destroyed stem vestige in rhizome. The ratio of appearance of multi-stem per plant and destroyed stem vestige in rhizome are increased according to root age.