오갈피나무속(屬)(Acanthopanax) 식물의 번식(繁殖)에 관한 연구(硏究) II. 종자형질(種子形質)과 배(胚) 발육(發育)특성

안상득,Ahn, Sang-Deuk 한국약용작물학회 1993 한국약용작물학회지 Vol.1 No.1

오갈피속식물의 종자들은 파종(播種) 후 발아(發芽)하는데 오랜 시간을 필요로 한다. 이러한 원인(原圖)이 오갈피속 식물의 종자가 미숙배를 갖고 있기 때문인지, 만약 미숙배를 갖고 있는 종자라면 인위적인 후숙처리로 배(胚의) 발육(發育)이 어느 정도 촉진되는지를 구명하므로써 보다 효과적인 종자번식방법(種子繁殖方法)을 개발할 수 있을 것이다. 따라서 오갈피속 식물들의 종자형질(種子形質)을 조사하고 후숙처리기간중 배(胚)의 발달정도를 조사하였던 바 그 결과를 요약하면 다음과 같다. 1. 종자의 크기는 오갈피, 서울오갈피 종자가 가장 크고 지리산오갈피와 섬오갈피 및 오가나무의 순서로 작은 경향이었다. 2. 종피(種皮)의 개열정도(開裂程度)는 오갈피, 서울오갈피의 종피는 각질이 반경질(半硬質)로 연화(軟되化어) 종피의 열개현상을 보이지 않았으나 섬오갈피는 이들보다 각질성(角質性)으로 gibberlline처리구에서만 80%이상의 높은 열개현상(裂開現像)을 나타내었다. 3. 공시된 식물들의 후숙처리시 배(胚)의 발육은 gibberelline을 처리한 종자에서만 배(胚)의 발육이 신장되어 완전한 자엽(子葉)이 형성되고, 종(種)에 따라서는 procambium도 뚜렷이 관찰된 반면, 무처리(無處理) 종자(種子)에서는 생장속도가 매우 늦어 배가 어느 정도 신장하는데 불과하여 발아력(發芽力)을 기대할 수 없었다. 4. 오갈피속 식물종자의 후숙처리(後熟處理) 전(前) 및 후숙기간 중 배(胚)의 크기와 발달정도를 관찰한 결과 인삼종자와 같이 미숙배(未熟胚)를 갖고 있기 때문에 발아에 오랜 시간이 소요된다는 사실을 알 수 있었다.분함양(殿含粉量)은 수원(水原) 3호(號)가 55.9%로 가장 높았고 수원(水原) 5호(號)는 51.7%로 가장 낮게 나타났다. 아미노산 조성(組成)에서는 김제종(金堤種)과 수원(水原) 6호(號)가 비슷한 경향(傾向)을 보였고 수원(水原) 3호(號)는 김제종(金堤種)보다 대체로 낮은 경향(傾向)을 보였다.-3}\;M$의 $K^+$을 먼저 첨가하여 2시간 동안 progesterone 전환 반응을 실시한 후에 $10^{-4}\;M$의 $Cd^{2+}$을 첨가하였을 때 가장 높은 $11\{alpha}-hydroxyprogesterone$의 수득률을 얻을 수 있었다.감소를 일으키며, 이러한 adenosine 작용은 magnesium이온에 의존적임을 알 수 있었다.mg/dL, 여자의 경우 각각 133.4 mg/dL, 149.4 mg/dL, 150.0 mg/dL로 59세 이하에서만 여자가 높고 다른 연령 군에서는 남자가 높았으며 연령증가와 함께 유의성은 없으나 증가하는 경향을 보였다. 혈청 총 콜레스테롤농도의 평균은 $189.53{\pm}4.73 mg/dL$, $157.93{\pm}4.96 mg/dL$이었고 남자가 유의적으로 높았다(p<0.05). 남녀 각각 59세 이하, 60대, 70대 이상군에서 185.0 mg/dL, 199.3 mg/dL, 180.9 mg/dL과 161.4 mg/dL, 180.9 mg/dL, 164.8 mg/dL였다. $40\~50$대는 남자가 유의적으로 높았으며 Seeds of Acanthopanx plants need about 2 years to germinate after sowing even if full matured fruits were harvested. I leave a room for doubt that this phenomenon could be brought by the immatured embryo in seed. If it is true, stratification method for the seed propagation of Acanthopanax plants would be used more effectively to promote the growth of embryo in relatively short time. Before stratification, seeds were devided into two parts. One of them was treated for 24hrs in the concentration of $GA_3$ 100 ppm. After stratification of non-treated and treated seeds, seeds for microtechnique were taken on interval of one month for three months, and fixed in Farmer's solution. The seed sizes of Acanthopanax plants were in biggest order A. sessiliflorus, A. seoulense, A. chiisanensis, A. koreanum and A. sieboldianum. The dehiscence phenomenon of seed coat didn't show in most of the seeds that stratified, but A. koreanum only dehisced in seeds treated in $GA_3$ 100ppm. The embryos of the stratified seeds that treated in $GA_3$ showed nomal growth, complete cotyledons and procambium in hypocotyl in any species, but the non-treated seeds could not expect the germination after sowing due to poor growing of the embryo in all species. In view of the results so far achieved, it has taken long times from sowing to germinate because the seed of Acanthopanax plants has immatured embryo.

들꽃(Rhodiola elongata Fisch. et Mey) 種子의 發芽에 미치는 生長調節物質의 效果

안상득,Ahn, Sang-Deuk 한국자원식물학회 1993 한국자원식물학회지 Vol.6 No.2

This study was attempted to establish the propagation and cultivation methods of Rhodiola elongata Fisch. et Mey. as a part of development of northern plant resources. Seeds were treated and investingated at the 15, 20, 25$^{\circ}C$ and 100, 200, 400, 800ppm of the growth regulator $GA_3$, BA, and Kinetin. The results obtained are summarized as follows: Seeds of R. elongata were the most vigorously germinated at 15-20$^{\circ}C$, and rate of germination at 25$^{\circ}C$ decreased gradually. Germination rate of seeds that treated growth regulators was remarkably increased than non-treated ones, and $GA_3$ treatment among the growth regulators showed the best effects. The effective concentration of $GA_3$ for germination of R. elongata seeds was 400 ppm.

人蔘 世代促進에 關한 硏究 第1報. 人工氣象室 栽培人蔘의 腦頭發達에 關한 組織學的 硏究

Sang Deuk Ahn(安相得),Kwang Tai Choi(崔光泰),Chan Moon Chung(鄭燦文),Woo Saeng Kwon(權宇生) 한국육종학회 1985 한국육종학회지 Vol.17 No.4

This study was carried out to shorten the period of breeding by acceleration of ginseng generations. The situation of developing and growing of dormancy buds in 2-year-old ginseng being cultivated under phytotron and field condition was surveyed by microtechnique method to determine the proper time for the low-temperature treatment. Differentiation times of dormancy buds of ginseng being cultivated in phytotron and field were similar to each other. Dormancy buds were jutted out to epidermis of root in the early of June, and separated to inner and outer husk. And also, in these time stem tissue was differentiated in the dormancy bud. Development of stem and leaf in the bud was completed in the early of September, but organs of flower were completed in the middle of September. Growth of bud was accelerated after August, and Complete formation of the new bud was taken about 5 months after sprout had emerged.

오갈피나무속 (Acanthopanax) 식물의 번식에 관한 연구

안상득(Sang Deuk Ahn) 한국약용작물학회 1993 한국약용작물학회지 Vol.1 No.1

Seeds of Acanthopanx plants need about 2 years to germinate after sowing even if full matured fruits were harvested. I leave a room for doubt that this phenomenon could be brought by the immatured embryo in seed. If it is true, stratification method for the seed propagation of Acanthopanax plants would be used more effectively to promote the growth of embryo in relatively short time. Before stratification, seeds were devided into two parts. One of them was treated for 24hrs in the concentration of GA₃ 100 ppm. After stratification of non-treated and treated seeds, seeds for microtechnique were taken on interval of one month for three months, and fixed in Farmer`s solution. The seed sizes of Acanthopanax plants were in biggest order A. sessiliflorus, A. seoulense, A. chiisanenss, A. koreanum and A. sieboldianum. The dehiscence phenomenon of seed coat didn`t show in most of the seeds that stratified, but A. koreanum only dehisced in seeds treated in GA₃ 100ppm. The embryos of the stratified seeds that treated in GA₃ showed normal growth, complete cotyledons and procambium in hypocotyl in any species, but the non-treated seeds could not expect the germination after sowing due to poor growing of the embryo in all species. In view of the results so far achieved, it has taken long times from sowing to germinate because the seed of Acanthopanax plants has immatured embryo.

溫度 및 日長條件이 人蔘의 生育 및 開花에 미치는 影響

Sang Deuk Ahn(安相得),Chan Moon Chung(鄭燦文),Woo Saeng Kwon(權宇生) 한국육종학회 1986 한국육종학회지 Vol.18 No.3

To observe the effects of temperature and photoperiod on the growth and flowering habits of ginseng(Panax ginseng C. A. Meyer), two variants, Violet-stem and Yellow-berry variant, of 3-year-old ginseng were tested at various conditions like 4 photoperiod levels(8,10,12,14hrs.) in each 3 temperature levels(10,18,25℃). Among the traits of two variants investigated Characteristics of quantitative characters such as stem diameter, stem length, petiole length, leaf length and leaf width were generally shorted at high temperature(25℃), and were elongated at low temperature(10℃), but the growth at 18℃ condition showed the most healthy. Total chlorophyll contents in leaf were decreased along to temperature and photoperiodic hour rising. Especially, days to emergence of the sprout and days to flowering were significantly accelerated along to the temperature rising, but were not significant according to photoperiod treatments. Consequently, we thought that the flowering time of ginseng could be controled by temperature treatment when flowering time of two parents are different about 35 days.

지황의 배발생캘러스 배양에서 탄소원과 질소원이 체세포배형성에 미치는 영향

박상언(Sang Un Park),조동하(Dong Ha Cho),장병호(Byong Ho Chang),안상득(Sang Deuk Ahn),박철호(Cheol Ho Park),유창연(Chang Yeon Yu) 강원대학교 농업과학연구소 1993 강원 농업생명환경연구 Vol.5 No.-

자연광기상실에서 인삼생육에 대한 광질의 영향

박훈,이미경,안상득,Park, Hoon,Lee, Mee-Kyoung,Ahn, Sang-Deuk 고려인삼학회 1989 Journal of Ginseng Research Vol.13 No.2

묘삼을 분재배하여 인공기상실 $15^{\circ}C$ 자연광실에서 여러가지 색의 셀로판지를 차광재로로 5~10%의 투광율로 재배하였다. 상대근중9최종생근 중/최초 생근 중)은 청>녹>백>황>적의 순서였다. 지상부 생육 특히 경장은 청색 광하에서 가장 저조하였다. 상대근중은 경장과 부상관을 보였으며 이 관계에서 각 광질은 특수한 영역을 보여 청과 녹이 한 군, 적과 황이 다른 한 군으로 분리되었다. 경장은 주당엽 면적과 전체 또는 각개의 광질내에서 정상관을 보였는데 적색 광에서만 유의성이 없었다. 잎중의 엽록소 a 및 b의 함량 또는 엽록소 a/b 비율은 백>청>녹>적>황의 순이었으며 엽록소 a와 b간, 전엽록소와 a/b 비율간 a/b 비율과 엽록소 a간에 모두 고도의 유의 정상관을 보였다. 이상의 사실들은 청색 차광자재가 인삼생산에 유리할 것임을 보여준다. Ginseng plants in the 2nd year were grown under the shade (90-95%) of colored cellophane films at $15^{\circ}C$ in a natural light phytotron. Relative root grown (final/initial) was in decreasing order of blue, green, white, yellow and red. The growth of aerial part, especially stem length was poorest in blue light with relative root growth negatively correlated with stem length. In this relation each color showed specific domain. Stem length showed positive linear correlation with leaf area per plant in each and among light though it was not significant in red light. The content and ratio of chlorophyll a and b in leaf were in the decreasing order of White, Blue, Green, Red and Yellow. There is positive correlation between chlorophyll a and chlorophyll b, and between total chlorophyll and chl:a/b ratio. Blue color shade is expected to be beneficial for ginseng production.

증폭된 DNA 다변성에 의한 삼지구엽초(Epimedium koreanum)의 종내변이

유기억(Yoo Ki Oug),안상득(Ahn Sang Deuk),유창연(Yu Chang Yeoun),박경열(Park Kyung Yeol),임학태(Lim Hak Tae) 한국원예학회 1997 Horticulture, Environment, and Biotechnology Vol.38 No.2

모과의 성분에 관한 연구

박종철(Jong Cheol Park),이종일(Jong Il Lee),안상득(Sang Deuk Ahn),양한석(Han Suk Yang) 한국생약학회 1989 생약학회지 Vol.20 No.1

참당귀의 생육단계별 GA₃ , IAA 및 함량변화

조선행(Seon Haeng Cho),신국현(Kuk Hyon Shin),안상득(Sang Deuk Ahn) 한국약용작물학회 1994 한국약용작물학회지 Vol.2 No.1

This study was carried out to observe the changes in gibberellin(GA₃), indol-3 acetatic acid(IAA) and abscisic acid(ABA) contents known as plant growth regulators in the leaves of Angelica gigas and to get the basic information for the establishment of cultural practice for the inhibition of floral induction. Changes in GA₃, IAA and ABA contents were investgated at different growth stage in the young flag leaves of two year old plants. The contents of GA₃ showed the highest value by 4.87㎎/㎏ at early flowering stage, and those were gradually increased from vegetative stage to early flowering stage, but greatly decreased from early flowering stage to fruiting stage. The contents of IAA were also the highest by 2.27㎎/㎏ at early flowering stage. The contents of ABA were gradually increased along with the development of growth stage. In the fruiting stage those were the highest value by 0.37㎎/㎏ and in early flowering stage the contents were decreased temporarily.