전통옹기 전탕기를 이용한 황련해독탕(黃連解毒湯)의 수량(水量) 조절방법 연구

안미영 ( Mee-young Ahn ),박웅남 ( Woong-nam Park ),최훈 ( Xun Cui ) 한약응용학회 2017 한약응용학회지 Vol.17 No.2

Objectives : Decoction is a method of extraction by boiling herbal to dissolve the chemicals of the material. The decocting method is a pharmacological extraction method in Korea. When decocting herbal medicines, the volume of water is an important factor. The aim of this research was to determine the proper amount of water when decocting herbal medicines. Methods : When water boiled without herbal medicines, the amount of water evaporation was measured. The total volume of water is determined by the amount of herbal water absorption, extraction and the water evaporation. Results : When the total amount of water was below 200ml, the water evaporation was 40%. Therefore the water evaporation was measured by this formula [(extraction + water evaporation) × 0.4] Conclusion : When the amount of herbal medicines increased, the volume of water should be increased also.

잇몸의 전반적인 증식을 초래하는 질환

안미영(Mee-Young Ahn),윤정훈(Jung-Hoon Yoon) 대한치과의사협회 2014 대한치과의사협회지 Vol.52 No.12

Generalized gingival enlargement, also known as gingival hyperplasia or hypertrophy, is defined as an abnormal diffuse overgrowth of gingival tissues. There are several causes of generalized gingival enlargement and they can be grouped into four categories: hereditary gingival fibromatosis, medication induced, inflammatory, and systemic or neoplastic causes of gingival enlargement. This paper reviews the clinical features, differential diagnosis and significance of generalized gingival enlargements.

추출용액에 따른 유산균 발효 땅두릅의 항염증 효과

우영민(Young Min Woo),김옥주(Ok Ju Kim),조은솔(Eun Sol Jo),조민영(Min Young Jo),안미영(Mee Young Ahn),이상현(Sang-Hyeon Lee),하종명(Jong-Myung Ha),김안드레(Andre Kim) 한국생명과학회 2018 생명과학회지 Vol.28 No.12

본 연구는 추출 용매별 땅두릅 추출물과 Lactobacillus plantarum으로 발효한 후 용매별로 추출한 땅두릅 발효추출물을 이용하여 항염증 효과를 측정한 연구이다. 땅두릅은 중추신경 계통에 대한 흥분작용이 있고 혈압강하 작용이 알려져 있으며 관절염, 감기, 신경통, 류마티스, 피부가려움증 등에 쓰인다. LPS (lipopolysaccharide)로 염증을 유도한 마우스 유래 macrophage에서의 NO 생성 및 염증관련인자의 발현에 미치는 영향을 검토하여 항염증 소재로서의 이용 가능성을 확인하였다. 건조 땅두릅을 water, ethanol, hexane, ethyl acetate, butanol을 이용하여 추출한 추출물들과 L. plantarum으로 발효한 후 추출한 추출물들에서 RAW264.7 대식세포에 대한 독성 여부를 측정하였고, 세포에 대한 독성이 나타나지 않는 농도에서 추출물의 항염증 활성을 확인하였다. 본 실험에 사용된 추출물은 LPS로 유도된 NO 생성을 유의적으로 억제하였으며, 주요 염증 유발인자인 COX-2와 iNOS의 발현 또한 유의적으로 억제하는 효과를 나타냈다. 염증관련 cytokine인 IL-1β, IL-6 및 TNF-α의 생성량 또한 유의적으로 감소시켰다. 이상의 결과로부터 땅두릅을 L. plantarum으로 발효한 후 water, ethanol, butanol로 추출하였을 때 염증억제 효과가 있는 기능성 식품 소재로서의 개발 가능성이 있을 것으로 사료된다. We investigated the anti-inflammatory activities of various organic solvent extracts with and without Lactobacillus plantarum fermentation of Aralia continentalis Kitagawa which has hypotensive effects in addition to excitatory effects on the central nervous system. It has been used to treat arthritis, colds, neuralgia, rheumatism, and itchy skin. Our extracts were tested for their anti-inflammatory potential on NO production and the expression of inflammatory factors in lipopolysaccharide-stimulated RAW264.7 macrophages. Extracts with and without L. plantarum fermentation were prepared using water, ethanol, hexane, ethyl acetate, and butanol. The RAW264.7 cells were tested for toxicity and the anti-inflammatory activity of each extract was determined at a concentration with no toxicity to the cells. The extracts used in this study significantly inhibited both the production of NO and the mRNA expression of COX-2 and iNOS, the major inflammatory factors. The production of inflammation-related cytokines IL-1β, IL-6, and TNF-α was also significantly reduced. These results suggest that the extracts involving fermentation by L. plantarum can inhibit cytokines by controlling the expression of inflammatory cytokine genes. It is considered that the water, ethanol, and butanol extracts after fermentation with L. plantarum could be useful as functional natural materials with anti-inflammatory effects.

추출 용매에 따른 Lactobacillus plantarum 발효 우슬의 항염증 효과 증진

조은솔 ( Eun Sol Jo ),우영민 ( Young Min Woo ),김옥주 ( Ok Ju Kim ),조민영 ( Min Young Jo ),안미영 ( Mee Young Ahn ),이재화 ( Jae-hwa Lee ),하종명 ( Jong-myung Ha ),김안드레 ( Andre Kim ) 한국공업화학회 2019 공업화학 Vol.30 No.2

본 연구에서는 우슬(Achyranthes japonica, AJ)과 Lactobacillus plantarum으로 발효한 우슬(AJ-LP)을 5가지 용매(물, 에탄올, 헥산, 아세트산에틸, 부탄올)로 추출하여 RAW264.7 세포에서 항염증 활성에 미치는 영향을 확인하였다. Lipopolysaccharide (LPS)로 유도된 RAW264.7 세포에서 nitric oxide (NO) 및 cytokine 생성을 측정하였으며 western blot으로 cyclooxygenase-2 (COX-2)와 nitric oxide synthase (iNOS)의 발현 정도를 관찰하였다. 세포 독성은 CCK assay를 통해 확인하였으며 독성을 가지지 않는 농도인 100 μg/mL로 실험을 진행하였다. NO 생성 저해 활성 결과 AJ-LP를 에탄올(E)로 추출한 시료가 LPS만 처리한 control군에 비해 약 74%로 저해율이 가장 높았으며 염증관련 cytokine인 Interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and Interleukin-1β (IL-1β)의 결과에서도 약 57, 70, 74%의 저해율로 우수한 효능을 보였다. COX-2와 iNOS 발현결과 AJ군에서 대조군(20-hydroxyecdysone)의 저해율이 가장 높은 것으로 나타났다. AJ-LP군에서 COX-2 발현량은 헥산(H)으로 추출한 시료가 대조군에 비해 약 16%로 감소하였고 iNOS 발현량은 부탄올(B)으로 추출한 시료가 약 7%로 감소하였다. 이상의 결과들에서 우슬보다 L. plantarum으로 발효한 우슬이 천연물 소재로서의 활용 가능성이 높음을 시사하였다. In this study, we used extracts obtained from five different solvents (water, ethanol, hexane, ethyl acetate, butanol) of Achyranthes japonica (AJ) and also AJ fermented with Lactobacillus plantarum (LP) to confirm effects on the anti-inflammatory activity in RAW264.7 cells. Experiments of measuring nitric oxide (NO) and cytokine production were performed in lipopolysaccharide (LPS)-induced RAW264.7 cells, and the expression of both cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) was observed by a western blot method. The cytotoxicity of RAW264.7 was confirmed by the cell counting kit (CCK) assay at a concentration of 100 μg/mL, which has no toxicity. As a result of the inhibition of NO production, the inhibition rate of AJ-LP extracted with ethanol samples was about 74% higher than that of using the control group. Interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and Interleukin-1β (IL-1β), which are inflammatory cytokines, also showed an excellent efficacy with inhibition rates of about 57, 70, and 74%, respectively. Comparing to the results of COX-2 and iNOS expression in the AJ group, the inhibition rate of 20-hydroxyecdysone was the highest than others. On the other hand, the COX-2 expression level of AJ-LP group decreased about 16% compared to that of the control group, and the iNOS expression level was also decreased about 7%. These results suggest that the extract of AJ fermented from L. plantarum can be used as an anti-inflammatory natural material.

가토의 장관골 골 결손 부위에 이식한 골막 유래 줄기 세포의 골 형성 유도

김휘택(Hui Taek Kim),윤평주(Pyung Ju Yun),김인보(In Bo Kim),이동호(Dong Ho Lee),안미영(Mee Young Ahn),장희경(Hee Kyung Chang) 대한정형외과학회 2006 대한정형외과학회지 Vol.41 No.2

목적: 미분화 줄기 세포를 골 조직 유도 촉진제 사용 없이 직접 동종 이식 시 골 형성의 정도를 알아보았다. 대상 및 방법: 성장 중인 백색 가토를 대상으로 경골 부분 절제술 후 이식 물질에 따라 제1군(줄기 세포와 agar), 제2군(agar), 제3군(골 절제술만 시행)으로 나누었다. 8주에 걸쳐 매주 단순 방사선 검사 후 가토를 희생시켜 신생 골의 조직 검사를 시행하였다. 형성된 골 조직이 이식한 줄기 세포에 의한 것임을 증명하고자 줄기 세포를 CM-Dil로 표시시켜 골세포에서 형광 발현 여부를 확인하였다. 결과: 골수와 인접한 부위에서 섬유 모세포와 골모세포로 분화되는 세포들이 전자현미경 상 관찰되었다. 골막 및 골수 강에서 유래한 골세포에 의한 연골내 골화와 줄기 세포에서 분화된 골모세포에서 형성된 유골이 함께 관찰되었다. 이식 6주째까지 형광을 발현하는 세포를 선생 골 조직 내에서 관찰할 수 있어 새로 형성된 골은 이식한 줄기 세포에서 기원함을 알 수 있었다. 결론: 단순 줄기 세포에 의한 골 형성 능력은 골 결손에 있어 골유합을 유도하려는 경우 향후 적용이 가능할 수 있을 것으로 생각된다. Purpose: To determine if stem cells transplanted directly into a bone defect of rabbit tibias have osteogenic induction potential. Materials and Methods: Immature white New Zealand rabbits underwent tibial osteotomies, and were divided into three groups according to the implant material used: stem cells embedded in agar (group 1); agar alone (group 2); nothing (group 3). For all rabbits, radiographs were taken weekly for 8 weeks, and histological studies of the newly formed-bone were performed. CM-Dil was used to label the stem cells prior to transplantation to ascertain whether or not the newly formed bone was derived from the transplanted stem cells. Results: Fibroblasts and osteoblasts (osteoid matrix-forming cells) derived from the stem cells were identified by electron microscopy. Interspersed enchondral ossification (probably induced by osteogenic cells from the remaining periosteum and marrow) and pure osteoids (created directly from the osteoblasts originating from the transplanted stem cells) were identified. Fluorescent-labeled cells were conspicuous in the new bones until 6 weeks after surgery, which indicates that the new bones were induced by the stem cells. Conclusion: The osteogenic induction potential of the undifferentiated stem cell has promise for therapeutic application, which may be used for the treatment of bone defects in the future.

개조개 및 대합 발의 점액질에 대한 조직화학적연구

안미영,조운복 부산대학교 사범대학 1992 교사교육연구 Vol.25 No.-

This experiment was performed to study the structure and the histochemical properties on the mucosubstances of the foot in Saxidomus purpuratus and Meretrix lusoria. To observe the structure of foot, hematoxylin-eosin staining was used. The mucosubstances were stained PAS, alcian blue pH 2.5, alcian blue pH 1.0, aldehyde fuchsin pH 1.7, aldehyde fuchsin pH 1.0, alcian blue pH 2.5,-PAS, alcian blue pH 1.0-PAS and aldehyde fuchsin pH 1.7-alcian blue pH 2.5 methods. The foot of Saxidomus purpuratus and Meretrix lusoria was composed basically of three layers ; epithelium, subepithelial connective tissue and muscular layers. The foot of both species was lined by simple ciliated columnar epithelial cells, and foot glands consisted of multicellular mucous acini were present in the subepithelial connective tissue. Especially the foot glands of Saxidomus purpuratus were large spheroid in shape and were numerously distributed within connective tissue, but these glands of Meretrix lusoria were small spheroid in shape and were scattered. The ducts of these glands extended within connective tissue and between epithelial cells, and open onto the surface of the foot. Especially the ducts of these glands of both regions developed in Saxidomus purpuratus than in Meretrix lusoria. Muscularis consisted of smooth muscle fibers. The muscle fibers were arranged as longitudinal, transverse and vertical layers. Acidic strongly sulfomucins were present within cilia and cytoplasm of foot epithelium in both species, but these mucins were more abundant in Saxidomus purpuratus than in Meretrix lusoria. The foot glands Saxidomus purpuratus were mixed two types of mucous acini along the affinity of two dyes, such as alcian blue and aldehyde fuchsin. Most mucous acini of these glands were stained with two dyes and were contained small to moderate amounts of strongly sulfomucins. While a few mucous acini of these glands were only stained with aldehyde fuchsin and contained small to moderate amounts of strongly sulfomucins. The ducts of the foot glands contained only strongly sulfomucin and were present in greater amounts in Saxidomus purpuratus than in Meretrix lusoria.