蚯蚓, 水蛭, 蠐螬 및 蜈蚣이 血栓症에 미치는 影響

安圭錫(Kyoo-Seok Ahn) 대한한의학회 1990 대한한의학회지 Vol.11 No.2

2천년대의 한의학

안규석(Ahn Kyoo Seok) 한의병리학회 1988 동의생리병리학회지 Vol.3 No.-

건각탕(健脚湯) 섭취가 엘리트 장거리 달리기 선수의 유산소성 능력 향상에 미치는 영향

이정필,정희정,안규석,오재근,최영진,Lee, Jeong-Pil,Jung, Hee-Jung,Ahn, Kyoo-Seok,Oh, Jae-Keun,Choe, Yeong-Jin 대한동의생리학회 2006 동의생리병리학회지 Vol.20 No.6

The purpose of this study was to identify the effects of oriental herbal drink to improve aerobic capacity in elite endurance runners. 14 male elite college runners were participated and divided into two group; i ) oriental herbal drink group (OG), ii ) placebo group (PG). All subjects were completed treadmill exercise protocol using GXT at before (B) and after (A) experimental treatment of one week. The V02max, anaerobic threshold (AT) were measured by gas analysis and heart rate (HR) were measure by polar system at pre, max, post, post 30 min and post 60 min. Blood samples were collected to analyze blood components. The V02max were significantly increased in OGA compared to OGB whereas the V02max and AT in OGA were significantly higher than PGA. The blood lactate concentration were shown higher decrease rate in OGA compared to Doth OGB and PGA during recovery whereas LDH and Na were significantly increased in OGA compared to both OGB and PGA. The blood concentrations of CI and K were significantly increased in OGA compared to PGA. There were no significant differences were founded in WBC, RBC, Hct, Hb and other components associated with energy sources(glucose, TG, TC, HDL, LDL, creatinine, CPK). These results suggested that this oriental herbal drink can be administrated to improve aerobic capacity in long distance runners.

혈부축어탕(血府逐瘀湯)이 암전이(癌轉移) 억제(抑制)에 미치는 영향(影響)

이진화,심범상,안규석,최승훈,Lee, Jin-Hwa,Shim, Bum-Sang,Ahn, Kyoo-Seok,Choi, Seung-Hoon 대한암한의학회 1999 大韓癌韓醫學會誌 Vol.5 No.1

To examine the effect of Xuefuzhuyutang on the metastasis of cancer, the following experiments were carried out. Before the main experiments, the cytotoxicity was measured by putting Xuefuzhuyutant sample in HT1080. Then zymography was made to examine the change of gelatinolytic activity. Western blotting was carried out to examine the changes of Fos, Jun, Ets, Erk, md JNK. In vitro invasion assay with transwells coated by collagen and matrigel was carried out. From the above results the following conclusions were obtained. 1. The experimental result about cytotoxicity of Xuefuzhuyutang agaitst HT1080 was a below. The stained cell count after being treated by by Xuefuzhuyutang sample $400{\mu}g/ml$ for 24 hours was 0.9% of total cells, and the stained cell count by Xuefuzhuyutang sample $100{\mu}g/ml$ was 1.5% of total cells. Both were near the level of control group which showed 0.6% stained. 2. The result of collagenase assay was as below. In Xuefuzhuyutang sample $400{\mu}g/ml$, MMP2 was reduced as compared with TPA control group, and the band of MMP-9 induced by TPA disapappeared. In Xuefuzhuyutang samle $800{\mu}g/ml$ both bands of MMP-2 and MMP-9 disapeared. 3. The results of western blots for Jun, Fos, Ets, Erk, JNK were a below. In Xuefuzhuyutang sample $200{\mu}g/ml$, Ets was reduced, and Jun, Fos were increased. 4 The result of invasion assay was as below. The number of cells which migrated across transwell membrane in Xuefuzhuyutang-treated group was less than that of control(+TPA) group. From the above results, it was concluded that Xuefuzhuyutang might inhibit the activity of collagenase not by the MMP-2, MMP-9 promoter but by the other way.

가미자도환(加味慈桃丸) 구성약물(構成藥物)의 혈관신생(血管新生) 억제효과(抑制效果)에 관한 연구(硏究)

왕덕중,심범상,안규석,최승훈,Wang, Teh-Chung,Shim, Bum-Sang,Ahn, Kyoo-Seok,Choi, Seong-Hoon 대한암한의학회 2002 大韓癌韓醫學會誌 Vol.8 No.1

Jiaweicitaowan (JWCTW) has been used to inhibit recurrence and metastasis of cancer in clinical practice, Further study has shown its anti-metastatic and anti-angiogenic effects. By applying in vitro and in vivo anti-angiogenic evaluation model, the author assayed the each ingredients of JWCTW. The author performed the following experiments and the results are listed as below: 1. Cell viability assay showed that the viability was almost identical between that of the control and that of the ingredients extract $40{\mu}g/ml$ treated, except of hexane fraction of Curcumae Radix $(40{\mu}/ml,\;2.0\%\;of\;control)$, ethyl acetate fraction $(40{\mu}g/ml,\;26.7%)$, butanol fraction $(20{\mu}g/ml,\;87.2%;\;40{\mu}g/ml,\;12.5%)$ of Cremastrae appendiculatae Tuber, water fraction of Persicae Semen $(40{\mu}g/ml,\;82.7%)$, ethylacetate fraction of Hippocampus $(40{\mu}g/ml,\;85.3%)$. 2. The results of gelatin zymogram assay showed that the ingredients of JWCTW decreases the gelatinolytic activity of MMP-9 from ECV304, at the concentration of $10{\mu}g/ml$. 3, In in vitro invasion assay, the ingredients of JWCTW effectively inhibited the invasion of cancer cells as compared with the control (+PMA) groups. 4. In capillary-like tube formation assay, the hexane and ethylacetate fractions of Curcumae Radix, Cremastrae appendiculatae Tuber and Persicae Semen showed the dramatic inhibition effects on tube formation of ECV304 at the concentration of $40{\mu}g/ml$. 5. In ex vivo rat aortic ring assay, the hexane and ethyl acetate fractions of Curcumae Radix, Cremastrae appendiculatae Tuber and Persicae Semen showed the inhibition effects on angiogenesis of rat aorta at the concentration of $40{\mu}g/ml$. According to the above research, the anti-angiogenic effects of the ingredients of JWCTW was proved and it suggested that the more effective prescription for anti angiogensis could be developed.

입안산(立安散)이 Angiogenesis 억제기전(抑制棋戰)에 미치는 영향(影響)

이기룡,최승훈,안규석,Lee, Gi-Ryong,Choi, Seung-Hoon,Ahn, Kyoo-Seok 대한암한의학회 1998 大韓癌韓醫學會誌 Vol.4 No.1

This experimental study was carried out to evaluate the effect of Yipahnsan on angiogenic inhibition mechanism. This study investigates the effects of Yipahnsan on angiogenic inhibition mechanism evaluate cell adhesive inhibition effect, DNA fragmantaion analysis, nuclear condensation assay, FACScan analysis, angiogenic lumen formation assay, immunocytochemistry analysis, RT-PCR for mRNA expression, western blot analysis, confocal analysis for $Ca^{2+}influx$. The results were summarized as follows : 1. The cell adhesive inhibition ability was strongly increased from $5{\mu}g/ml$ on ECV304 cell line and ECVPAR cell line. 2. YY water extract caused $G_0/G_1$ arrest peak to existed on the ECV304 cell line. 3. YY water extract caused inhibition of proliferation and inducement of apoptosis on the collagen coated plate in ECV304 cell line. 4. YY water extract inhibited the lumen formation on the matrigel coated plate in ECV304 cell line. 5. YY water extract inhibited the expressions of LFA-1 and ELAM-1 on ECV304 cell line and ECVPAR cell line. 6. YY water extract inhibited the expressions of MMP-9 and uPA on ECV304 cell line and ECVPAR cell line. 7. YY water extract inhibited the expression of integrin ${\alpha}_v{\beta}_3$ on ECV304 cell line and ECVPAR cell line. 8. YY water extract decreased the change of $Ca^{2+}$ in intracellular on ECV304 cell line and ECVPAR cell line. According to the results, Yipahnsan showed to be a key antagonist of integrin ${\alpha}_v{\beta}_3$, and to be induction of apoptosis by p53 through flow cytometry. This report also demonstrated that expressions of MMP-9 and uPA were blocked under the angiogenesis model. Thus, we suggested that Yipahnsan blocks angiogenesis by inducing apoptosis in ECV304 and ECVPAR cell lines, and another oriental herbal medicine that treats qi-stagnation and blood-stasis type also has angiogenic inhibition effects.

한양방결합요법에 의한 자가면역성 만성 활동성 간염, 고빌리루빈혈증 1례 보고

양주노,최승훈,안규석,심범상,Yang, Ju-No,Choi, Seung-Hoon,Ahn, Kyoo-Seok,Shim, Bum-Sang 대한암한의학회 2005 大韓癌韓醫學會誌 Vol.10 No.1

Objective: This study was done to treat autoimmune chronic active heaptitis Jaundice patient. Method: I analyzed the medical records of a case of autoimmune chronic active hepatitis Jaundice who had been treated with korean traditional medicines from 1 December 2003 through 6 July 2004. Result: Following the korean traditional medicine method, during taking medicine the hematological markers (aspartate transferase(AST), alanine aminotranferase(ALT), Total bilirubin(T.bili)) were effectively controled.

반지련으로 부터 분리한 luteolin의 세포고사효과

이은옥,김진형,안규석,박영두,김성훈,Lee, Eun-Ok,Kim, Jin-Hyung,Ahn, Kyoo-Seok,Park, Young-Doo,Kim, Sung-Hoon 대한동의생리학회 2005 동의생리병리학회지 Vol.19 No.3

We previously demonstrated that the methylene chloride fraction of Scutellaria barbata suppessed human leukemic U937 cell proliferation by inducing apoptosis. In the present study, we have isolated luteolin from Scutellaria barbata and evaluated its apoptotic mechanism in Lewis lung carcinoma cells. Luteolin inhibited the proliferation of Lewis lung carcinoma cells in a concentration-dependent manner. Luteolin effectively increased the portion of $sub-G_1$ DNA content (apoptotic portion) and apoptotic Annexin-V positive cells in a concentration-dependent manner by FACS analysis. Caspase 9 and caspase 3 were activated and PARP was effectively cleaved by luteolin. It also increased the ratio of Bax to Bcl-2 through the decrease of Bcl-2 expression by Western blotting and reduced mitochondrial membrane potential following TMRE staining. These results suggest that luteolin can induce apoptosis through the mitochondrial mediated pathway.

활락효령단(活絡效靈丹)이 Angiogenesis 억제기전(抑制機轉)에 미치는 영향(影響)

나기환,최승훈,안규석,Na, Ki-Whan,Choi, Seung-Hoon,Ahn, Kyoo-Seok 대한암한의학회 1998 大韓癌韓醫學會誌 Vol.4 No.1

This experimental study was carried out to evaluate the effects of Hwallakhyoreungdan on angiogenic inhibition mechanism. In order to investigate the effects of Hwallakhyoreungdan on angiogenic inhibition mechanism, MTT assay, cell adhesive inhibition effect, DNA fragmantaion analysis, Nuclear condensation assay, FACScan analysis, Angiogenic lumen formation assay, Immunocytochemistry analysis, RT-PCR for mRNA expression, Western blot analysis and Confocal analysis for $Ca^{2+}$ change were performed. The results were summarized as follows: 1. The cell adhesive inhibition ability was strong from $5{\mu}g/ml$. 2. The $G_0/G_1$ arrest peak was existed on ECV304 cell-line. 3. The cells on Collagen plate were inhibition of proliferation and inducement of apoptosis by HR water extract. 4. Angiogenic lumen formation was inhibited by HR water extract. 5. LFA-1 and ELAM-1's expression were inhibited by HR water extract. They are commenly participation on inflammation and tumor regeneration. 6. The expression of MMP-9 and uPA were inhibited by HR water extract. 7. The expression of integrin ${\alpha}_v{\beta}_3$ was inhibited by HR water extract. 8. The expression of intracellular molecule were successively inhibited by HR water extract therefore the proliferation of ECV304 cell line was stopped and apoptosis was induced. 9. The change of $Ca^{2+}$ was decreased by HR water extract it cause confusion of signal transduction pathway therefore it was take part in apoptosis. According to the results, Hwallakhyoreungdan showed to be a key antaonist of integrin ${\alpha}_v{\beta}_3$, and to be induction of apoptosis by p53 through flow cytometry. This report also demonstrated that expressions of MMP-9 and uPA was blocked under the angiogenesis model. Thus, we suggests that Hwallakhyoreungdan blocks angiogenesis by inducing apoptosis of ECV304 and ECVPAR cell lines and another oriental herbal medicine that treats blood-stasis type also has angiogenic inhibition effects.

십전대보탕(十全大補湯)이 암전이(癌轉移) 억제(抑制)에 미치는 영향(影響)

윤재호,최승훈,안규석,Yoon, Jae-Ho,Choi, Seung-Hoon,Ahn, Kyoo-Seok 대한암한의학회 1998 大韓癌韓醫學會誌 Vol.4 No.1

To examine the effect of Shiquandabutang on the metastasis of cancer, the following experiments were made. Before the main experiments, the cytotoxicity was measured by putting Shiquandabutang sample in HT1080. Then zymography was made to examine the change of gelatinolytic activity. And western blotting was carried out to examine the changes of Fos, Jun, Ets, the transcription factors of MMP-2, MMP-9, and Erk, JNK on signal transduction pathway to AP-1. Third, in vitro invasion assay with transwells coated by collagen and matrigel was carried out. From the results of the above the following conclusions were obtained. 1. The experimental result about cytotoxicity of Shiquandabutang against HT1080 was as below. The stained cell count after being treated by Shiquandabutang sample $400{\mu}g/ml$ for 24 hours was 0.9% of total cells, and the stained cell count by Shiquandabutang sample $100{\mu}g/ml$ was 1.5% of total cells. Both were near the level of control group which showed 0.6% stained. 2. The result of collagenase assay was as below. In Shiquandabutang sample $400{\mu}g/ml$, MMP-2 was reduced as compared with TPA control group, and the band of MMP-9 induced by TPA disappeared. In Shiquandabutang sample $800{\mu}g/ml$, both bands of MMP-2 and MMP-9 disappeared. 3. The results of western blots for Jun, Fos, Ets, Erk, JNK were as below. In Shiquandabutang sample $200{\mu}g/ml$, Ets was reduced, and Fos were increased. 4. The result of invasion assay was as below. The number of cells which migrated across transwell membrane in Shiquandabutang-treated group was less than that of +TPA control group. From the above results, it was concluded that Shiquandabutang might control the appearing and acting of collagenase not by the MMP-2, -9 promoter but by other way.