LC/ESI-MS/MS를 이용한 수질 환경 시료 중 산성의약물질 분석방법 비교

심영은(Young-Eun Sim),조현우(Hyun-Woo Cho),명승운(Seung-Woon Myung) 한국분석과학회 2008 분석과학 Vol.21 No.3

알코올의존증 치료약물 복용 여부 모니터링을 위한 소변 중 크레아티닌 농도로 보정된 날트렉손 및 6β-날트렉솔 측정값 평가

심영은(Yeong Eun Sim),김선영(Seon Yeong Kim),정재철(Jae Chul Cheong),이현수(Hyun Soo Lee),표재성(Jaesung Pyo),김진영(Jin Young Kim) 대한약학회 2022 약학회지 Vol.66 No.2

This study aimed to detect naltrexone (NTX) and its metabolite 6β-naltrexol (6βNTX) in the urine samples of parolees or probationers who were legally bound to participate in an alcohol dependence treatment program to determine whether to follow the medical treatment ordered by the court. The developed liquid chromatography-tandem mass spectrometry method was suitable for the simultaneous detection of NTX as well as its active metabolite 6βNTX in urine samples. The ratio of creatinine to the analyte ([analyte, ng/mL]/[creatinine, mg/dL]) was used to exclude inaccuracies caused by urine dilution, and this value was further corrected by the average urine creatinine concentration in Korean adults (male 132.6 mg/dL, female 93.3 mg/dL). The cut-off values for NTX and 6βNTX were set at 10 ng/mL. If both the parent drug and its metabolite were found in urine and the corrected creatinine concentrations of NTX and 6βNTX were more than 10 ng/mL, it was considered that the participants were following their treatment protocol accordingly. The results showed that 81 (94.2%, male 69 and female 12) of 86 participants were taking NTX, whereas five (four males, one female) were not adhering to the protocol accordingly. It was found that the four male participants (4.9%) of the 81 participants who were taking the drug initially had urinary NTX and 6βNTX concentrations below the cut-off level without correcting creatinine level, but the values met the acceptance criteria (i.e., >10 ng/mL) of the medical treatment program after correcting the creatinine level. The proposed method can be applied to other drugs in the future.

수양명경근(手陽明經筋)의 해부학적(解剖學的) 고찰(考察)

심영,박경식,이준무,Sim Young,Park Kyoung-Sik,Lee Joon-Moo 경락경혈학회 2002 Korean Journal of Acupuncture Vol.19 No.1

This study was carried to identify the component of Large Intestine Meridian Muscle in human, dividing into outer, middle, and inner part. Brachium and antebrachium were opened widely to demonstrate muscles, nerve, blood vessels and the others, displaying the inner structure of Large Intestine Meridian Muscle. We obtained the results as follows; 1. Meridian Muscle is composed of the muscle, nerve and blood vessels. 2. In human anatomy, it is present the difference between a term of nerve or blood vessels which control the muscle of Meridian Muscle and those which pass near by Meridian Muscle. 3. The inner composition of meridian muscle in human arm is as follows. 1) Muscle; extensor digitorum tendon(LI-1), lumbrical tendon(LI-2), 1st dosal interosseous muscle(LI-3), 1st dosal interosseous muscle and adductor pollicis muscle(LI-4), extensor pollicis longus tendon and extensor pollicis brevis tendon(LI-5), adductor pollicis longus muscle and extensor carpi radialis brevis tendon(LI-6), extensor digitorum muscle and extensor carpi radialis brevis mucsle and abductor pollicis longus muscle(LI-7), extensor carpi radialis brevis muscle and pronator teres muscle(LI-8), extensor carpi radialis brevis muscle and supinator muscle(LI-9), extensor carpi radialis longus muscle and extensor carpi radialis brevis muscle and supinator muscle(LI-10), brachioradialis muscle(LI-11), triceps brachii muscle and brachioradialis muscle(LI-12), brachioradialis muscle and brachialis muscle(LI-13), deltoid muscle(LI-14, LI-15), trapezius muscle and supraspinous muscle(LI-16), platysma muscle and sternocleidomastoid muscle and scalenous muscle(LI-17, LI-18), orbicularis oris superior muscle(LI-19, LI-20) 2) Nerve; superficial branch of radial nerve and branch of median nerve(LI-1, LI-2, LI-3), superficial branch of radial nerve and branch of median nerve and branch of ulna nerve(LI-4), superficial branch of radial nerve(LI-5), branch of radial nerve(LI-6), posterior antebrachial cutaneous nerve and branch of radial nerve(LI-7), posterior antebrachial cutaneous nerve(LI-8), posterior antebrachial cutaneous nerve and radial nerve(LI-9, LI-12), lateral antebrachial cutaneous nerve and deep branch of radial nerve(LI-10), radial nerve(LI-11), lateral antebrachial cutaneous nerve and branch of radial nerve(LI-13), superior lateral cutaneous nerve and axillary nerve(LI-14), 1st thoracic nerve and suprascapular nerve and axillary nerve(LI-15), dosal rami of C4 and 1st thoracic nerve and suprascapular nerve(LI-16), transverse cervical nerve and supraclavicular nerve and phrenic nerve(LI-17), transverse cervical nerve and 2nd, 3rd cervical nerve and accessory nerve(LI-18), infraorbital nerve(LI-19), facial nerve and infraorbital nerve(LI-20). 3) Blood vessels; proper palmar digital artery(LI-1, LI-2), dorsal metacarpal artery and common palmar digital artery(LI-3), dorsal metacarpal artery and common palmar digital artery and branch of deep palmar aterial arch(LI-4), radial artery(LI-5), branch of posterior interosseous artery(LI-6, LI-7), radial recurrent artery(LI-11), cephalic vein and radial collateral artery(LI-13), cephalic vein and posterior circumflex humeral artery(LI-14), thoracoacromial artery and suprascapular artery and posterior circumflex humeral artery and anterior circumflex humeral artery(LI-15), transverse cervical artery and suprascapular artery(LI-16), transverse cervical artery(LI-17), SCM branch of external carotid artery(LI-18), facial artery(LI-19, LI-20)

간 기능 이상 치료에 대한 C.M.R.T. 치료 부위(T8 횡돌기)와 경혈과의 비교

심영,이준무,Sim Young,Lee Jun-Moo 경락경혈학회 2002 Korean Journal of Acupuncture Vol.19 No.2

Chiropractic is very similar to Oriental Medicine in philosophy on the cause of diseases and in utilization of spinal articulations for diagnosis and treatment. In this paper the spinal area used to treat liver dysfunction in S.O.T. technique, one of chiropractic techniques, was compared to the acupncture points used to cure the same conditions. Because both Oriental medicine and Chiropractic are dealing with autonomic nervous system in regulating abnormal conditions, also the innervation of spinal nerves to those areas was checked. The spinal area that S.O.T. technique utilizes to correct liver dysfunction is transverse processes of T8, which corresponds to B16. Acupncture points from this level down to T12/L1, which are B16, B17, B18, B19, B20, B21, B45, B46, B47, B48, B49, B50, GV6, GV7, GV8 and GV9, all have been applied to control liver function. Apparent discrepency exists in therapeutic areas for liver malfunction between the two natural healing arts. According to the neurology texts, liver is innervated by sympathetic fibers from the 7th-10th thoracic segments and by parasympathetic fibers from vagus nerve. Sympathetic afferent nerves from the liver reach the 7th-12th thoracic spinal cord segments. It can be said all the 7th-12th thoracic spinal cord segments are related to liver function. Therefore the areas used for liver dysfunction in both natural medicine are appropriately selected. However, B16, the Oriental medical equivalent of the main spinal area which is used for lowered liver function in C.M.R.T. Technique, is not utilized as frequent as in Oriental medicine.

소변 시료 중 메스암페타민 및 암페타민 광학이성질체 농도 측정 결과의 불확도 평가

김진영(Jin Young Kim),심영은(Yeong Eun Sim),고범준(Beom Jun Ko) 대한약학회 2019 약학회지 Vol.63 No.6

The objective of this study was to assess the measurement uncertainty for determination of methamphetamine and amphetamine enantiomers in urine sample by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The deuterium-labeled compounds were prepared for use as internal standards to quantify the analytes. Urine sample (200 μl) was diluted with pure water before the solid phase extraction (SPE). The sample was extracted and purified using Oasis HLB SPE cartridge followed by chromatographic separation on a vancomycin-based chiral column (150 mm×2.1 mm, 5 μm). The mass spectrometric analysis were performed in multiple reactions monitoring (MRM) selecting three precursor/ fragment ion pairs for each analyte. The potential sources that contribute uncertainty to the final result were evaluated. The major contributing factors impacting the combined standard uncertainty were calibration curve and repeatability, while the preparation of standard solution and sample dilution were minor factors. Relative expanded uncertainty of the measured analyte concentration was within 8.5% in a forensic urine sample.

생육시기 및 부위별 고추의 항산화력 및 항암 Lunasin peptide의 동정

권기수,박재호,김대섭,정진부,심영은,김미숙,이희경,정규영,정형진,Kwon Ki Soo,Park Jae Ho,Kim Dae Seop,Jeong Jin Boo,Sim Young Eun,Kim Mi Suk,Lee Hee Kyung,Chung Gyu Young,Jeong Hyung Jin 한국생명과학회 2005 생명과학회지 Vol.15 No.4

부위간의 DPPH free radical scavenging 활성은 과육은 종자 보다 높았고 생육시기간에는 후기가 초기에 비하여 높았고, Xanthine oxidase 억제활성은 종자와 과육에 비하여 매우 높았고 동정된 항 산화물은 대부분이 지방산 및 페놀성 화합물로, 과육 및 종자가 성숙 될수록 1-eicosanol, palmitic acid, linoleic acid, linolenic acid 등의 지방산 및 benzonitrile의 함량이 증가되었다. 시료 채취 시기간의 POD활성은 과육은 성숙중기에 높았으나, 종자는 성숙이 경과될수록 낮았다. SOD 활성은 과육은 생육이 경과할수록 증가하였으나, 종자는 성숙할수록 낮아졌다. 고추종자 단백질은 western blot에 의하여 lunasin이 동정되었고, 개화 후 5주부터 lunasin 생합성 되었다. 고추 종자내의 100nM lunasin 펩타이드는 종양유발 유전자가 전이된 $2\~12$ 세포의 콜로니 형성을 억제시켰으며, 결과적으로 고추 종자내에는 암 예방적 특성을 갖는 lunasin peptide가 존재한다. The non-enzymatic anti-oxidants and lunasin peptide from the extracts of the pepper were examined in order to utilize the discovery in natural products as cancer chemopreventive agents. The DPPH (1,1-diphenyl-2-picryl-hydrazyl) free radical scavenging activity on the fruit parts of the pepper was higher than that of the seed, but the difference was low. The Inhibition activity of xanthine/ xanthine oxidase in extracts of the seed was higher than that of the fruit and that of the seed on 20 days after flowering was the highest at the growing period. These were identified as fatty acids and phenolic compounds such as 1-eicosanol, palmitic acid, linoleic acid, linolenic acid and benzonitrile. The contents of fatty acids and phenolic compounds increased according to the time passing at the growing period. Peroxidase (POD) activity of the fruit at middle stage was high than that of other growing stages and that of the seed was the highest at later growing period. Though superoxide dismutase (SOD) activities in fruit were hish by passage of Slowing stage, the activity in seed was low. Lunasin was searched from seeds of the peppers by coomassie blue staining and western blot among them and we just found lunasin peptide from extracted protein of the pepper by western blot. In addition, we observed the contents of lunasin after flowering and confirmed to appear the lunasin at 35 days after flowering. We confirmed that lunasin is complex protein of maturing seeds. 100nM lunasin peptide in pepper showed inhibition effect on colony formation in $2\~12$ cells.

SCNCrM-2B와 SM25C의 마찰용접특성에 관한 연구

이세경(Se-Gyoung Lee),심영만(Young-Man Sim),민택기(Taeg-Ki Min) 한국생산제조학회 2006 한국생산제조학회지 Vol.15 No.4

차광재배가 눈개승마(Aruncus dioicus var. kamtschaticus)의 성분 및 항산화 특성에 미치는 영향

권정우(Jeong Woo Kwon),박재호(Jae-Ho Park),권기수(Ki Soo Kwon),김대섭(Dae Seup Kim),정진부(Jin Boo Jeong),이희경(Hee Kyung Lee),심영은(Young Eun Sim),김미숙(Mi Suk Kim),윤지용(Ji Young Youn),정규영(Gyu Young Jeong),정형진(Hyung Jin J 한국자원식물학회 2006 한국자원식물학회지 Vol.19 No.1

눈개승마의 총 엽록소, 총 카로테노이드 함량은 무차광에 비하여 차광율이 높을 수록 높았다. 지방산함량은 무차광 재배에 비하여 차광 재배 및 식용 가능시기간의 생육 후기에 감소되었다. 식용가능시기에서의 지방산 종류간의 함량은 oleic acid, palmitc acid, linoleic acid, stearic acid 순으로 높았으며, 무차광에 비하여 차광율이 높을 수록 낮았다. 특히 linoleic산의 함량은 타 지방산에 비하여 차광정도에 따른 함량의 감소율이 매우 높았다. 차광율 및 수확시기간의 휘발성 정유성분의 함량은 차광율이 높을 수록 증가하였고, 초기수확이 높았다. DPPH 소거활성은 6.64-14.49(IC50:㎍/㎖로 항산화 활성이 매우 높게 나타났고 차광정도가 높을 수록 낮았고, 조기 수확 할 수록 높았다. POD 및 SOD 활성은 식물체에 비하여 종자가 매우 낮았고, 무차광에 비하여 차광 재배 시에 증가하였다. POD활성은 식용가능시기의 생육 초기에 비하여 후기에 높았으나, SOD활성은 감소되었다. POD Isozyme band 수는 이식묘의 종자, 잎 및 줄기에서 각 각 6개의 밴드를 나타내었으나, 뿌리에는 없었다. SOD Isozyme band는 송자와 잎에 비하여 뿌리에서 뚜렷하였다. This study was conducted to determine chemical compounds and antioxidant in Aruncus dioicus. Further, effects of cultural practices such as the shading conditions on the general compounds, essential oils and antioxidants. The chemical compounds and antioxidant activity of edible extracts on the shading cultivation and harvesting time were measured by crude fiber, pigments, higher fatty acids, essential oils and DPPH free radical scavenging ability and activities of SOD and POD in edible shooting parts of Aruncus dioicus. The contents of crude fiber, total chlorophyll and total carotenoids in extracts of edible shooting by shading cultivation and later harvesting were higher than those of non-shading cultivation and early harvesting. Phenolic compounds such as benzoic acid were identified as the aromatic compounds in the edible extracts of Aruncus dioicus. The contents of azulene, benzaldehyde and linalool among those compounds on the cultivation conditions increased in shading cultivation. IC50 values of DPPH free radical scavenging activities were high from 6.644 to 14.499m㎍/㎖ in extracts of edible parts and those of edible shooting parts was high such as edible shooting cultivated by 60% shading, 30% shading and non-shading, respectively. The activity of POD and SOD in seeds was lower than that of edible shooting and that by shading cultivation was high in extracts cultivated by non-shading. POD activity of extracts on harvesting time was high in earlier harvesting but SOD activity was low. The numbers of isozyme pattern of POD and SOD in seedling showed 7 bands and 3 bands, respectively, especially, bands of POD and SOD in the first year-growing plant did not show and show a difference according to plant positions, respectively.

가계의 저축 및 차용행태에 관한 연구 (1)

심영(Young, Sim) 서원대학교 미래창조연구원 1997 응용과학연구 Vol.6 No.1

간 기능 이상 치료에 대한 C.M.R.T. 치료 부위(T8 횡돌기)와 경혈과의 비교

이준무 ( Jun Moo Lee ),심영 ( Young Sim ) 대한경락경혈학회 2002 Korean Journal of Acupuncture Vol.19 No.2

Chiropractic is very similar to Oriental Medicine in philosophy on the cause of diseases and in utilization of spinal articulations for diagnosis and treatment. In this paper the spinal area used to treat liver dysfunction in S.O.T. technique, one of chiropractic techniques, was compared to the acupncture points used to cure the same conditions. Because both Oriental medicine and Chiropractic are dealing with autonomic nervous system in regulating abnormal conditions, also the innervation of spinal nerves to those areas was checked. The spinal area that S.O.T. technique utilizes to correct liver dysfunction is transverse processes of T8, which corresponds to B16. Acupncture points from this level down to T12/L1, which are B16, B17, B18, B19, B20, B21, B45, B46, B47, B48, B49, B50, GV6, GV7, GV8 and GV9, all have been applied to control liver function. Apparent discrepency exists in therapeutic areas for liver malfunction between the two natural healing arts. According to the neurology texts, liver is innervated by sympathetic fibers from the 7th-10th thoracic segments and by parasympathetic fibers from vagus nerve. Sympathetic afferent nerves from the liver reach the 7th-12th thoracic spinal cord segments. It can be said all the 7th-12th thoracic spinal cord segments are related to liver function. Therefore the areas used for liver dysfunction in both natural medicine are appropriately selected. However, B16, the Oriental medical equivalent of the main spinal area which is used for lowered liver function in C.M.R.T. Technique, is not utilized as frequent as in Oriental medicine.