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유통 한약재 중 내분비계 장애물질로서의 잔류농약에 관한 연구(I)
김도훈,김혜수,오미현,강인호,심영훈,황완균,명승운,최병기,조정희,Kim, Do-Hoon,Kim, Hye-Soo,Oh, Mi-Hyune,Kang, In-Ho,Shim, Young-Hun,Hwang, Wan-Kyun,Myung, Seong-Wun,Choe, Byung-Ki,Cho, Jung-Hee 한국생약학회 2000 생약학회지 Vol.31 No.4
Research on pesticide residues as endocrine disruptors in natural medicines was initiated by Korea Food & Drug Administration this year. We determined the presence and levels of certain pesticides in selected natural medicines. The natural medicines collected this year are Glycyrrhiza Root, Cinnamon Bark, Pueraria Root, Polygonatum Rhizome, Jujube, Schizandra Fruit, Lycium Fruit, Liriope Tuber, Eucommia Bark, Peony Root, Korean Angelica, Dioscorea Rhizome, Cnidium Rhizome, Cassia Seed, Platycodon Root, Comus Fruit, Mentha Herb, Epimedium Herb, Bupleurum Root, and Ginger, which have no data for pesticide residues and 192 samples of them were circulated in Korea, 28 samples were circulated in China. In order to analyze many pesticides in large number of samples we used simultaneous multi-residue analysis of pesticides by GC-ECD, which was followed by GC-MSD analysis to confirm the identity of the detected pesticide in each sample.
조정희,김도훈,오미현,강인호,심영훈,김은경,조창희,지선경,원도희,김영중,문영희 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-
튿은 도라지 ,PfgfycodoH gian겉친orum A. De Candole 의 뿌리 또는 주피를 제거한 것으로 한방 제제에서 분즈파뼘 및 트늦촛랸 등의 효능으로 널리 사용되는 한약재이다. 현재 길경 함유 제제는 방풍통성산, 오적산 등 100꺽'품목치 허가되어 있으며 약전규격시험으로는 확인시험, 회분 및 엑스함량이 설정되어 있으나, 함랸시험은 설정되어 있지 않다. 따라서 본 연구에서는 생약 및 생약제제(한방제제) 의 품질규격화를 위하여 국내, 중국 및 일본 길경 유통품 30종을 확보하여 형래즉적 감별 및 약전규격 시험을 실시하였고, TLC법에 의한 확인시험법과 지표성분을 이용한 기기분석법에 의한 항량시험법을 확립하였다. 약전규격시끌으로서 회분, 엑스함량을 시험한 결과 회분은 1.4~4.0% (기준 : 4.0% 이하)이며 엑스함량은 쪼.3~T4.4% (기준 :25.0% 이상)으로 모두 적합하였다. 확인시험법을 검토한 결과 메탄올추출액을 흡착제 : Silicagel 9el GF2%, 전개용매 .클로로포름 ·메탄을 ·물 (6 :4 : 1), 발색제 : 10% 알코올성황산시액의 초건으로 박층크로마토그래프법에 파라 시험하였을 때 표준품 및 각 원료에서 가장 좋은 패턴을 나타내어 확인시험법으로 가장 적합하였다 함량시험의 경우 지표물질로 platycodin D에 대해 검토한_켤과 HPLC법으로는 피크의 검출이 용이하지 않았으며, HPTLC법에 따라 시험했을패 흡착제 .옥타데실실릴화한 실리카겔, 전개용매 :물 ·메탄을 sodium dodecyl sulfate (20 · 20 : 0.5), 발색제 : 10% 에탄올섣황산시액, 검출파장 : 450nm의 조건에서 최적의 결과를 나타내었다. 각 즌역별 유통품 30종에 대하여 platycodin D 함량을 측정한 결과는 0.48~l,24%(0.79±0.20%)이었고, 또한 길경단미엑스, 방풍통성삯, 오적산, 패독산, 인삼패독산 및 삼소음 등 길경함유제제 중 plat)·codin D의 추출이행률을 검토한 결과 45.g~93.2%(보.2±17.8%)의 추출이행률을 나타내었다. 본 시험법으로 시판되는 길경함유제제인 형개연교탕, 인삼패독산 제제즉 대해 시험한 결과 0.18~l,24%를 나타내어 제제에서도 적용가능할 것으로 사료되었다. This study carried out the standardization and quality control of Platycodon Root. It is osed in traditional meacines to relieve diarrhea, constipation, bloating, low energyIrveas, and damage from parasites. Also it is used for its expectorant qualities to dilate thebronchii. Platycodon Root contains vafous compounds of saponins such as platycodin A, B,0,D2, D3, polygalacln D, f:』 and platycodigenin, phytosterol such as α -spinasteroT, o -spinasterolglucoside, sfigmasta-7-fnol and inulin as polysaccharide. In this study, the contents ofplatycodin D as a standard compound are 0.48~l.24% with 30 samples collected from diflerentareas. Total ash content of Platycodon Root samples Is 1.4 ~4.0% and EtOH ertrac4 content is25.3 ~34.4%. The identification method was evaluated by TLC and quantitative method byHPTLC for Platycodon Roo'4. The proper conditions of 7LC were , absorbent : Silicagel gelGp2s4, solvent : chloroform · afeOH · HaO (6 : 4 : 1), spraying reagent : 10% HBSOf. To fuautifyPlatycodon Root, HPTLC me·thed was applied and the optimal analytical conditions of HPTLCwere as follows , absorbent : Cls, solvent : H2O · hfeOH · sDs (20.20 : 0.5), spraying reagent :10% H2s04, used wavelengtil : 450nm. The availability of platycodin D in water extract ofPlatycodon Root preparations was 45.9 ~93.2% and the contents of platyrodin D in PlatycodonRoot preparations in market such af In-sam-par-dok~ san and Hyung-kye- yun-kyo-tang were0.24% and 0.18%, respectively.
조정희,김도훈,오미현,강인호,심영훈,김은경,김종환,김달환,지선경,김현주,하광원,김영중,문영희 식품의약품안전청 2001 식품의약품안전청 연보 Vol.5 No.-
의약분업과 더불어 한받엑스쓱립제에 대한 수요가 증가추끼에 밌다. 따라서 본 연구에서는 딴이 사용되는 생약(한약)제제에 대하여 지표물질의 이행률 및 안정성을 검토하고 지표물질에 의한 한량시극법을 확립하고자 하였다 백삼을 한유하는 제제중 식품의약품안전청고시에 수재된 가미귀비랑엑스과킬등 10품목과 수재되지 않은 대건중탕엑스과립 등 j픔목에 대하여 탕저를 실험걸적으로 조제하고 갈근 감초. 계지. 목단피, 백작약, 진괴, 백삼, fl자. 황금, 황련에 대하여 원료생약 및 탕제에 대한 함량시극출 한 다음 각 지표성분의 이행률을 검토하였다. 각 탕제별 백잠의 함량시험은 진세노사이드 Rbi을 ·i표성분으로 하여 시험하였고, 진끼노사이드 Rbl의 이행를은 제제에 따라 fS.6~71.4ff의 범위플 나타네었다. 본 시험결과를 기초로 하여 제제중 백삼의 함량시험법을 고시에 추가 설정하고, 레건증탑엑스곽립제 등을 고시에 수재하는 등 향후 고시의 제 · 개정에 반영하고 씽약(한약)제제의 합리적인 푼질판피를 확립하고자 한다 The demands of herbal medicine preparations have been increased along )ITiti) the growing interest about healthcare and the separation of pharmacy and clinic.'rhereforethis study Ivas carried out to examine the availability of marker and its stability toward uTidel),-used herbal medicine rreparations. Effective component of Ginseng Radix Atba is kno)t·n asPana,tadiol such as girisenoside Rbl, Rbf, Rgl, etc. Using the standard compounds, puerarine.gl!'cIFrrhiElc acid, cinnamic acid, paeoniflorin, geniposide. baicatin, and berberlne, Ive investigatedthe 10 noticed preparations (ex. Gamiguibi-tang) and S unnotired preparations (ex. Daegunjung-tang) in KFDA NOTICE. And the content of Ginseng aadi,』 Alba f·as examined based oilavailabilittT of ginsenoslde Rbl. As a result, the contents of ginsenoside Rbl u·as 2i.6 to 71.4%from Ginseng Radix #Llba in each herbal medicine preparation. This exT)eriment made tl)epossibilitl'of content tes4 of Ginseng Radi.1 Alba b:#· ginsenoside Rbl In herbal medicinepreparation.
유통 한약재의 내분비계장애물질 모니티링 : 유통한약재 중 잔류농약에 관한 조사연구 Studies on Pesticide Residues in Natural Medicines
강인호,조정희,김도훈,심영훈,김은경,김종욱,황완균,최호영 대한본초학회 2002 大韓本草學會誌 Vol.17 No.2
Objectives : Research on pesticide residues as endocrine disruptors in herbal medicines was initiated by Korea Food & Drug Administration in 1999. The presence and levels of certain pesticides in selected herbal medicines samples were determined. Forty species of herbal medicies which have no background data on pesticide residues including Osterici Koreani Radix were collected this year. Methods : 276 samples out of total 427 were circulated in Korea, and the others were in China. In order to analyze many pesticides in large number of samples, simultaneous multi-residue analysis of pesticides by GC-ECD was used. The GC-MSD analysis was followed to confirm the identity of the detected pesticide in each sample. Results and conclusions : The frequencies of pesticide detection in natural medicines were 18 samples circulated in Korea and 12 samples circulated in China. The range of pesticide residues for DDT was 0.007~0.09ppm in 8 samples. That for lindane was 0.001~0.02ppm in 7 samples, for endosulfan was 0.001~0.074ppm in 6 samples, for methoxychlor was 0.004~0.161ppm in 5 samples, for chlordane was 0.006~0.02ppm in 4 samples. The range of pesticide residues for parathion was 0.001ppm in 1 samples.
한약재 유해물질 모니터링 사업(Ⅰ) : 유통 한약재의 중금속에 관한 연구 Studies on heavy metals of natural medicines in market
김종욱,최호영,조정희,김도훈,강인호,심영훈,김은경,Myung, Seung Woon 대한본초학회 2002 大韓本草學會誌 Vol.17 No.2
Objectives : This study was performed to determine the harmful heavy metal ions(Pb, Hg, As, Cd, Cr) in twenty species of natural medicines circulated in Korea and China. Methods : The levels of heavy metal ions(Pb, As, Cd, Cr) were processed using kjeldahl digestion and ICP(Inductively Coupled Plasma spectometer). Mercury was determined by the amalgamation method. Results : Lead was detected less than 0.01(㎍/g) in most samples but in Achyranthes Root it was detected more than 1.0(㎍/g). The amount of Mercury was 0.01(㎍/g) in Trichosanthis Radix and 0.14(㎍/g) in Lonicera Flower. However, in Osterici Koreani Radix of Seong-do(China), Coix Seed of Jecheon(Korea) and Seoul(Korea) and Cimicifuga Rhizome of Seong-do(China), it was detected more than 1.2(㎍/g). The amount of Arsenic was determined 0.12(㎍/g) in Peach Kernel and 0.89(㎍/g) in Cimicifuga Rhizome. Cadmium was detected similar levels in most samples. Cadmium levels in Asiasarum Root was higher than any other samples and its value was 0.40(㎍/g). The amount of Chromium was detected 0.26(㎍/g) in Coix seed and 4.1(㎍/g) in Asiasarum Root. Chromium levels in Osterici Koreani Radix, Achyranthes Root and Asiasarum Root were higher than others. Hazardous heavy metals except Cr were detected less than 1.0(㎍/g) in most samples. Conclusion : The results mean that natural medicines were exposed to risk-hazardous environment.