백리향 (Thymus quinquecostatus Celakovsky) 잎에 분포하는 두상형 분비모의 미세구조

신현철,유성철,Shin, Hyun-Chur,Yu, Seong-Cheol 한국현미경학회 1998 Applied microscopy Vol.28 No.2

The glandular secretory system of the capitate gandular trichomes in leaf of Thymus quinquecostatus Celakovsky was examined by transmission electron microscope. The glandular trichome was consisted of three cell layers; an basal cell layer, a stalk cell with single-celled intermediate layer and a discoid secretory layer with thickened cuticle. The secretory cell was dense, rich in mitochondria, rER, plastds, Golgi complex and had many vesicular structure. Typical plastids with reticulate body and plastoglobule were present in glandular trichome. The tytoplasm of secretory cell was filled with osmiophilic secretory materials. The secretory vesicles, originated from Golgi complex, appeared as membrane bounded vesicles and secreted to the outer wall surface. The presences of well developed rER, mitochondria, Golgi complex, and membrane-bounded vesicles fused with plasmalemma in the secreting cells indicate that the granulocrine mechanism of secretion was occurring in T. quinquecostatus. Subcuticular cavity was developed between the cuticular layer and the secretory cell wall, and it formed above the secretory cell upon separation of cuticle-wall.

흰쥐에서 체감각유발장전위의 기록부위별 특성과 경로분석

신현철,박용구,이배환,류재욱,조춘식,정상섭,Shin, Hyun Chul,Park, Yong Gou,Lee, Bae Hwan,Ryou, Jae Wook,Zhao, Chun Zhi,Chung, Sang Sup 대한신경외과학회 2001 Journal of Korean neurosurgical society Vol.30 No.7

Objective : Somatosensory evoked potentials(SSEPs) have been used widely both experimentally and clinically to monitor the function of central nervous system and peripheral nervous system. Studies of SSEPs have reported the various recording techniques and patterns of SSEP. The previous SSEP studies used scalp recording electrodes, showed mean vector potentials which included relatively constant brainstem potentials(far-field potentials) and unstable thalamocortical pathway potentials(near-field potentials). Even in invasive SSEP recording methods, thalamocortical potentials were variable according to the kinds, depths, and distance of two electrodes. So they were regarded improper method for monitoring of upper level of brainstem. The present study was conducted to investigate the characteristics of somatosensory evoked field potentials(SSEFPs) of the cerebral cortex that evoked by hindlimb stimulation using ball electrode and the pathways of SSEFP by recording the potentials simultaneously in the cortex, VPL nucleus of thalamus, and nucleus gracilis. Methods : In the first experiment, a specially designed recording electrode was inserted into the cerebral cortex perpendicular to the cortical surface in order to recording the constant cortical field potentials and SSEFPs mapped from different areas of somatosensory cortex were analyzed. In the second experiment, SSEPs were recorded in the ipsilateral nucleus gracilis, the contralateral ventroposterolateral thalamic nucleus(VPL), and the cerebral cortex along the conduction pathway of somatosensory information. Results : In the first experiment, we could constantly obtain the SSEFPs in cerebral cortex following the transcutaneous electrical stimulation of the hind limb, and it revealed that the first large positive and following negative waves were largest at the 2mm posterior and 2mm lateral to the bregma in the contralateral somatosensory cortex. The second experiment showed that the SSEPs were conducted by way of posterior column somatosensory pathway and thalamocortical pathway and that specific patterns of the SSEPs were recorded from the nucleus gracilis, VPL, and cerebral cortex. Conclusion : The specially designed recording electrode was found to be very useful in recording the localized SSEFPs and the transcutaneous electrical stimulation using ball electrode was effective in evoking SSEPs. The characteristic shapes, latencies, and conduction velocities of each potentials are expected to be used the fundamental data for the future study of brain functions, including the hydrocephalus model, middle cerebral artery ischemia model, and so forth.

차세대 멀티미디어 이동통신 망에서의 이동성 제어 방법

신현철,장희선,Shin Hyun-Cheul,Jang Hee-Seon 한국정보기술전략혁신학회 2002 情報學硏究 Vol.5 No.4

본 논문에서는 이동통신망에서 동적 위치등록 관리 방안을 제시한다. 동적 위치등록 방안을 제어하기 위해서 VLR내에 가입자마다 동적인 원형 위치등록 방법을 이용한다. 가입자가 새로운 위치영역으로 이동시 시스템은 현재의 원형 위치정보에 포함되어 있는지를 판단하고 이전 VLR과 새로운 VLR에 가입자의 위치정보를 갱신하게 된다. 아울러 가입자의 위치정보를 동적으로 관리하기 위해 발신호 설정시 현재의 가입자의 위치를 통보한다. 제안된 방법을 이용하여 이전의 방법 보다 HLR에서의 위치등록 트래픽을 줄일 수 있음을 보여준다.

TWINSPAN 및 DCCA 에 의한 한반도 주목림의 군락과 환경의 상관관계 분석

신현철,이강영,송호경 ( Hyun Chul Shin,Kang Young Lee,Ho Kyung Song ) 한국산림과학회 1998 한국산림과학회지 Vol.87 No.4

This study was carried out for the purpose of supplying the basic data for artificial forestation, natural regeneration and ecological conservation etc., and obtaining information on alpine vegetation, by establishing vegetation units on the basis of phytosociological classification of community and studying growth pattern on the basis of species composition, hierarchy structure and population dynamics, for Taxes cuspidata naturally growing at the alpine districts in Korea. The importance value of Taxus cuspidata by districts mostly showed above 100 in the upper story but at Mt. Chiri and Mt. Hanra communities its value was comparatively low. In the middle story it showed lower value than that of upper story and not quite showed at Mt. Chiri. Taxus cuspidata communities in the lower story were only in Mt. Hanra, Mt. Odae and Mt. Sobaek, and their importance values were about 10 which were relatively low values. The communities were classified into five groups as Taxus cuspidata-Males baccata var. mandshurica, Taxus cuspidata-Abies nephrolepis, Taxus cuspidata-Abies koreana, Taxus cuspidata-Acer mono and Taxus cuspidata-Euonymus quelpaertensis by TWINSPAN analysis. Taxus cuspidata-Abies koreana community was distributed at the northern aspect of the mountain ridges and at higher elevation than other communities and distributed. Taxus cuspidata-Acer mono community was relatively low than the others and distributed at the hillsides of mountain. And Taxus cuspidata-Euonymus quelpaertensis communities were distributed at the relatively high elevation and northern and eastern aspect of the mountain tap, and Taxus cuspidata-Malus baccata var. mandshurica communities were distributed at the medium elevation, and southern and eastern aspect of the mountain ridge. In the relation between communities and environmental factors, it was correlated with aspect, elevation and topography at the first axis, and elevation, slope at the second axis.

노인(老人) 내과환자(內科患者)의 임상적(臨床的) 특성(特性)에 대(對)한 고찰(考察)

신현철,정지천,심규박,Shin, Hyeon-Chul,Jeong, Ji-Cheon,Sim, Gyu-Bak 대한한방내과학회 1997 大韓韓方內科學會誌 Vol.18 No.1

This study is investigation of clinical statistics toward to old patients of 164 persons over full 65 years old to go to hospital in 5 internal medicine department of the Dongguk University Oriental Medicine Hospital for full one year from 1995. 8 to 1996. 7. The results are followings ; 1. The ratio of male and female was 1:1.6, and in offspring figures five persons or more(60.3%) was most. A couple(32.3%) was extremely much in residing forms, but in accordance to sexual distinction in male a couple(51.7%), in female solitude(31.0%) and an eldest son couple(30.0%) was far much. 2. An attack time, first cure was most in western hospital(59.2%), 81.1% of them desired western-oriental medicine cooperation, in a medical fee charging, themselves(50.7%) were far much, and most were affirmative(76.8%) against aging prevention of restorative. 3. Main diseases were cerebral nerve(41.5%) and circulatory system(25.6%), hypertention (23.8%, 9.8%) was most in complication and the past. In defecation constipation(31.7%), in urination frequence(46.7%) was far much. 4. In preventive measures of diseases invigorant(25.6%) and nothing(24.3%) was far much and in a person tending the sick a mate in male(16.7%), a son in female(13.0%) was extremely much. 5. In sexual life most of(91.5%) didn't act, a mate death was 53.1% and female was far much. And paralysis attack was extremely much in 65-74 years old(76.5%).

자생 상록 굴거리나무의 실내조명 하에서 적정광도 분석 및 도입방안

신현철,윤재길,최경옥,Shin, Hyeon-Cheol,Yun, Jae-Gill,Choi, Kyoung-Ok 한국조경학회 2012 韓國造景學會誌 Vol.40 No.1

본 연구는 우리나라 자생종 굴거리나무에 대한 실내조명 하에서 광적응성을 조사해 보고 실내조경공간으로 도입할 때, 도입방안을 제시해 보고자 수행되었다. 공시수종인 굴거리나무는 제주도 농가에서 1년생을 구입한 후, 경남과학기술대학교 종합농장 유리온실에서 40일간 순화시킨 후 2010년 2월 15일에서 2010년 11월 15일까지 실험하였다. 첫째, 형광등의 경우, 100lux에서 500lux까지 식물이 고사하거나 잎이 지는 현상을 보여 생육상태가 좋지 못하였으며, 1,000lux부터 원활한 생육 및 생장을 보였다. 엽수나 수형, 체내 광합성률, 관상가치 등을 토대로 살펴볼 때, 형광등 1,000lux에서 가장 양호한 생장을 하여 최적광도인 것으로 조사되었다. 둘째, LED등의 경우,100lux에서 1,000lux까지 고사현상을 보여 생육상태가 불량하였으며, 광도가 높을수록 원활한 생장을 보였다. 2,000lux에서 가장 원활한 생육 및 생장을 하였다. 셋째, 삼파장의 경우,1,000lux까지 고사현상이 심하여 도입한 4개의 광원 중 생육상태가 가장 좋지 않아 전반적으로 삼파장은 굴거리나무 생육에 부적합한 것으로 파악되었다. 2,000lux에서 가장 원활한 생육 및 생장을 하였다. 넷째, 광섬유의 경우 100lux하에서도 식물 전체가 고사하는 현상은 보이지 않아 생육이 가능하여 도입광원 중 생육 및 생장상태가 가장 양호하였다. 대체적으로 광도가 높을수록 생장률이 높았으나,2,000lux보다 1,000lux하에서 엽수의 출엽률 및 수형이 흐트러지지 않아 관상가치가 더 좋았으며, 체내 광합성률도 높아 1,000lux를 조명해 주는 것이 가장 효과적인 것으로 파악되었다. 광적응성을 고려하여 굴거리나무를 실내조경공간으로 도입할 때, 광섬유, 형광등, LED등의 순으로 도입하는 것이 적합하며, 삼파장 등은 부적합한 것으로 파악되었다. 광원별 최적광도는 광섬유와 형광등을 도입할 경우 1,000lux, LED등을 도입할 경우 2,000lux를 조명해 주는 것이 효과적인 것으로 나타났다. 또한, 자생종 굴거리나무는 외래종인 대엽홍콩을 대체할 수 있는 수종으로 실내조경식물로 도입이 가능하며, 중심목으로 활용할 수 있을 것으로 기대되었다. This study was performed to determine light-adaptability under indoor lighting for Korean wild Daphniphyllum macropodum, and to provide an introduction plan for indoor spaces of landscape architecture. The experimental plants, Daphniphyllum macropodum were purchased from a farmhouse in Jejudo as anannualplant, and it was acclimatized in the glass-green house of the general farm of Gyeongnam National University of Science and Technology for40 days. The experiment was performed from February 15, 2010 to November 15, 2010. First, in the case of the fluorescent lamp, the growth and development condition was poor because the plants withered, or the leaves fell off under the 100lux to 500lux, but the condition under 1,000lux was good In the aspects of the number of leaves, form of the tree, photosynthesis rate in its body, and the value of sight, the best light intensity for the growth condition was under the 1,000lux. Second, in the case of the LED light, the growth and development condition was poor because the plants withered, or the leaves fell off under 100lux to 1,000lux, but the smooth growth and development was done under a more light intensity. The best intensity for the growth condition was under 2,000lux. Third, in the case of the three-wave light, the withering was serious by 1,000lux, and the growth and development was the worst amongst the four introduced lighting systems, therefore, growth under the three-wave light was incongruous. The best intensity for the growth condition was under 2,000lux. Fourth, in the case of the optical fiver, the withering did not existed under 100lux and growth was possible. The growth and development was the amongst in the four introduced lighting systems. Generally, in the more light intensity, more growth was observed, but the value of sight was higher under 1,000lux than under 2,000lux because the falling rate of leaves and the form of the tree was stable. The most effective light was under 1,000lux due to the high photosynthesis in its body. When Daphniphyllum macropodum is introduced into the indoor landscaping space considering the light, the optical fiber, fluorescent lamp, and LED light are suitable to introduce. The three-wave light is unfit. The most proper light intensity by the light source, in the case of the optical fiber, and fluorescent lamp, is 1,000lux, and, in the case of LED light, is 2,000lux. The wild Daphniphyllum macropodum is the species of tree to substitute the Schefflera actinophylla which is the introduced species, and it is expected to be use as the central tree in indoor spaces.

방전 플라즈마 소결법에 의한 다공성 알루미나 세라믹스의 제조

신현철,조원승,신승용,김준규,Shin, Hyun-Cheol,Cho, Won-Seung,Shin, Seung-Yong,Kim, Jun-Gyu 한국세라믹학회 2002 한국세라믹학회지 Vol.39 No.12

고강도의 다공성 알루미나 세라믹을 제조하기 위하여, 기공 전구체로서 흑연을 첨가한 후, 방전 플라즈마 소결시의 제조조건을 체계적으로 조사하였다. 제조한 다공성 알루미나에 대하여, 기공률과 기공분포 및 압축강도 등을 조사하였다. 알루미나에 흑연을 10∼50 vol%까지 첨가하여 방전 플라즈마 소결법으로 1차 소결체를 제조한 후, 공기 중 흑연을 열분해시켜 최종적으로 다공성 알루미나를 제조하였다. 그 결과, 소결온도 1000${\circ}C$, 유지시간 3 min, 압력 30 MPa, 승온속도 80${\circ}C$/min, 펄스방법 12:2의 조건에서 높은 기공률의 다공성 소결체를 얻을 수 있었다. 일례로, 10∼30 vol%의 흑연을 첨가하여 제조한 다공성 알루미나의 경우, 약 50∼57%의 높은 기공률을 나타내었다. 또한, 흑연의 함량이 증가함에 따라 개기공률은 지속적으로 증가하였다. 이러한 기공특성과 흑연함량의 상호관계는 클러스터의 수와 크기에 의존하는 퍼콜레이션 모델에 의해 설명될 수 있다. 한편, 10∼30 vol%의 흑연을 첨가하여 제조한 다공성 알루미나의 경우, 55∼200 MPa의 높은 강도값을 나타내었다. 이와 같은 강도의 향상은 펄스전원에 의한 분말 입자간 방전 및 자기발열작용의 영향으로 생각된다. In order to develope the porous alumina ceramics with high strength, the pore characteristics and compressive strength were investigated in terms of relation to the conditions of spark-plasma sintering and the contents of graphite as a pore precursor. Porous alumina bodies were successfully prepared by spark-plasma sintering and burning out graphite in air. High porous bodies were fabricated by sintering at 1000${\circ}C$ for 3 min under a pressure of 30 MPa, heating rate of 80${\circ}C$/min and on-off pulse type of 12:2. For example, alumina bodies prepared by the addition of 10∼30 vol% graphite showed high porosity of 50∼57%. Also, the open porosity increased with graphite content. The relationship between pore characteristics and graphite contents could be explained by percolation model depending on cluster number and size. Porous alumina bodies prepared by the addition of 10∼30 vol% graphite showed the high compressive strength of 55∼200 MPa. This great improvement in strength was considered to be mainly due to the spark-plasma discharges and the self-heating action between particles.

아산시 일대 수생식물 분포와 수환경의 관계 - 침수식물을 중심으로

신현철,김영식,조강현,최홍근 ( Hyunchur Shin,Youngsik Kim,Kang Hyun Cho,Hong Keun Choi ) 한국하천호수학회 1997 생태와 환경 Vol.30 No.4

To understand the relationship between the distribution of submerged hydrophytes and water quality, the flora of hydrophytes and physiochemical characters of water, including temperature, dissolved oxygen, electric conductivity and pH, were investigated in Asan city, Chungnam, Korea. As the results, 18 taxa of hydrophytes were collected in Asan city. Among them, 5 taxa, Potamogeton crispus L., P. malaianus Miquel, Hydrilla verticillata(L. fil) Royle, Ceratophyllum demersum L. and Myriophyllum verticillatum L., were submerged hydrophytes, and their distributional patterns were not similar each other. Hydrilla verticillata and Ceratophyllum demersum were more euryhyric than others. Potamogeton crispus was distributed where showed more or less lower conduc-tivity and higher pH, whereas Myriophyllum verticillatum was where showed higher conductivity and lower pH.

Bacillus amyloliquefaciens H41이 생산하는 Vibrio anguillarum 생육 저해인자의 정제

신현철(Hyun-Chul Shin),정경태(Kyung-Tae Chung),김광현(Kwang-Hyun Kim),김병우(Byung-Woo Kim),권현주(Hyun-Ju Kwon),이은우(Eun-Woo Lee),염종화(Jong-Hwa Yum),류은주(Eun-Ju Rhu),정유정(Yu-Jeong Jeong),김영희(Young-Hee Kim) 한국생명과학회 2008 생명과학회지 Vol.18 No.6

어류 질병 치료를 위한 probiont의 개발에 목적을 두고 어병균 Vibrio anguillarum NCMB1의 생육에 저해물질을 생산하는 Bacillus amyloliquefaciens H41균주를 분리하고 이 물질의 특성을 규명하기 위하여 정제를 시행하였다. 분리 균을 배양한 배양 상등액을 70% 염석, 투석하여 조 효소액으로 제작하고 조 효소액을 DEAE-sephadex, A-50 ion exchange chromatography, sephadex G-200 gel filtration column chromatography을 통하여 정제하고 SDS-PAGE 를 통하여 단일밴드를 확인하고 최종 회수율 2.9%을 얻을 수 있었으며 40.8배의 정제된 V. anguillarum NCMB1 생육저해물질을 얻을 수 있었다. 정제된 저해물질은 저해정도에 따라 단위를 설정하여 활성을 측정하였으며, 분자량은 48 kDa 로 확인되었으며 정제물질의 활성을 위한 최적 반응 pH와 온도는 pH 7.5와 30℃로 확인되었다. 금속이온의 효과에 있어서는 CoCl₂, HgCl₂, ZnSO₄, AgNO₃에서는 완전히 저해되는 양상을 나타내었고 MgSO₄, MnSO₄에서 미미한 효소활성의 증가를 나타내었다. 그리고 염에 관한 안정성은 일반 해수의 농도인 3%의 농도에서도 활성을 나타내는 것으로 확인되었다. 정제된 저해물질을 현재 상업적으로 사용하고 있는 화학처리제나 항생제와 함께 효율성을 비교 해 보았을 때 저해물질은 약 78%의 저해 활성을 나타내는 것을 알 수 있었고 항생제보다는 효율성이 낮았으나 독성검사를 위해 정제물질을 살아있는 어류에 투여하였으나 어떤 해수어도 폐사하지 않는 것으로 보아 어류 자체엔 독성을 나타내지 않는 물질로 나타났다. 따라서 B. amyloliquefaciens H41 균주가 생산하는 정제 물질이 V. anguillarum 생육에 저해물질로 작용하는 것으로 밝혀졌으며 친환경적인 특성을 가진 물질로 밝혀졌다. To study the possible use of probiotics in fish farming, we evaluated antagonism of antibacterial strain Bacillus amyloliquefaciens H41 against the fish pathogenic bacterium Vibrio anguillarum NCMB1. The purification of growth inhibition factor produced by B. amyloliquefaciens H41 was achieved by obtaining supernatant of this bacterium. The growth inhibition factor was purified to homogeneity by 70% ammonium sulfate precipitation, DEAE-sephadex A-50 ion exchange chromatography, sephadex G-200 gel filtration column chromatography, and sephadex G-50 gel filtration column chromatography with 40.8 fold of purification and 2.9% yield. The molecular weight of the purified growth inhibition factor was 48 kDa as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The optimum pH and temperature for the growth inhibition factor were pH 7.5 and 30℃, respectively. The activity of growth inhibition factor was enhanced slightly by some metal ions, such as Mg?², Mn?², but was inhibited by the addition of Co?², Hg?², Zn?² and Ag?². NaCl stability of the growth inhibition factor was observed with 50% residual activity at 3% NaCl concentration. Toxicity test showed that the purified B. amyloliquefaciens H41 growth inhibition factor did not affect the live of Japanese flounder (Paralichthys olivaceus) and the effectiveness was 78% of residual lethality compared to commercial antibacterial agents.

한반도 비자나무림의 식생구조

신현철 ( Hyun Cheol Shin ),이광수 ( Kwang Soo Lee ),박남창 ( Nam Chang Park ),정수영 ( Su Young Jung ) 한국산림과학회 2010 한국산림과학회지 Vol.99 No.3