키토산올리고당이 에스트로겐 의존성 인간유방암세포 MCF-7의 전이활성에 미치는 영향

신진선 ( Jin Sun Shin ),이규식 ( Kyu Shik Lee ),남경수 ( Kyung Soo Nam ) 한국키틴키토산학회 2012 한국키틴키토산학회지 Vol.17 No.1

We investigated the effect of chitosan oligosaccharide (COS-L, MW < 1,000 Da) on metastatic activities in MCF-7 estrogendependent human breast cancer cells. In a concentration range of 0.1 to 5 mg/mL, aromatase transcription was significantly down-regulated by COS-L. In gelatin zymographic analysis, matrix metalloproteinase-9 (MMP-9) activity induced by phorbol- 12-myristate-13-acetate (TPA) was critically inhibited by COS-L in a dose-dependent manner. In contrast, tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) transcriptions was enhanced by COS-L but not changed TIMP-2. Therefore, the results suggest that COS-L should decrease the metastatic potential in MCF-7 estrogen-dependent human breast cancer cells through the decrease of aromatase transcription and MMP-9 activity and the increase of TMP-1 expression. In conclusion, present investigation provides critical information that COS-L may diminish the risk of mortality and recurrence caused by metastasis in estrogen-dependent breast cancer.

저분자 키토산올리고당이 인간 유방암 세포의 MMP-9 활성 및 TIMPs 발현에 미치는 영향

신진선 ( Jin Sun Shin ),이규식 ( Kyu Shik Lee ),남경수 ( Kyung Soo Nam ) 한국키틴키토산학회 2011 한국키틴키토산학회지 Vol.16 No.3

저분자 키토산올리고당(Mw ≤ 1,000)이 사람 유방암 세포인 에스트로겐 비의존성 MDA-MB-231 인간 유방암세포의 전이에 미치는 효과를 wound healing assay와 MMP-9의 활성 및 TIMP-1, TIMP-2 발현 변화를 통해서 조사하였다. 그 결과, 저분자 키토산올리고당이 MDA-MB-231 인간 유방암세포의 이동성을 억제하고 이 과정에서 MMP-9의 활성을 감소시키고 MMP-9의 조절 inhibitor인 TIMP-1의 발현을 증가시킴을 확인하였다. 이는 저분자 키토산올리고당이 MMP-9의 활성을 억제시킴으로써 종양의 기저막 및 세포외 기질의 분해를 저해하여 다른 조직으로의 전이를 예방할 수 있음을 보여주는 결과이다. 따라서 본 연구는 저분자 키토산올리고당이 에스트로겐 비의존성 유방암의 전이를 효과적으로 억제할 수 있는 소재로 활용이 가능하고 이를 통해 유방암의 치료 효과를 높여줄 수 있고, 나쁜 예후를 최소화할 수 있는 방안으로 활용이 가능함을 보여준다. We investigated the effect of low molecular weight chitosan oligosaccharide (COS-L, MW<1,000 Da) on metastatic activity in MDA-MB-231 human breast cancer cells. In a concentration range of 0.05 to 3 mg/mL, cell migration was significantly decreased by COS-L in a dose dependent manner. Matrix metalloproteinase-9 (MMP-9) activity induced by phorbol-12-myristate-13-acetate (TPA) was critically inhibited by COS-L at 3 mg/mL. In contrast, tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) transcription was dose-dependently increased. However, tissue inhibitor of matrix metalloproteinase-2 (TIMP-2) was not changed. The results suggest that the decrease of metastatic potential in MDA-MB-231 human breast cancer call by COS-L should be mediated by the inhibition of MMP-9 activity via the induction of TIMP-1. In conclusion, present investigation provides critical information that COS-L may diminish the risk of mortality and relapse caused by metastasis in breast cancer.

저분자 키토산 올리고당의 직장암 발생과정에의 영향

이상길 ( Sang Gil Lee ),신진선 ( Jin Sun Shin ),조정원 ( Cheong Weon Cho ),손윤희 ( Yun Hee Shon ),남경수 ( Kyung Soo Nam ) 한국키틴키토산학회 2010 한국키틴키토산학회지 Vol.15 No.4

저분자 키토산 올리고당(Mw<1,000)의 사람 직장암세포 HT-29에서의 QR과 GST의 활성 및 GSH 생성 유도효과와 ODC 활성에의 영향을 측정한 결과, 저분자 키토산 올리고당은 phase Ⅱ 생체 해독효소인 QR과 GST의 활성 증가 및 GSH 함량을 증가시켰으므로 직장암발생(colon cancer carcinogenesis)의 개시단계(initiation)를 저해할 수 있을 것으로 보인다. 또한 직장암 발생과정의 촉진단계(promotion)에 관여하는 ODC 활성도 저해하였으므로 저분자 키토산 올리고당은 직장암 발생의 개시단계와 촉진단계에 저해효과가 있을 가능성이 높다. 그러므로 저분자 키토산 올리고당은 직장암 발생과정에 관련된 더 많은 연구를 통하여 직장암발생 억제물질로서 개발 가능성이 있는 것으로 보인다. The effect of low molecular weight chitosan oligosaccharide (Mw<1,000) on colon cancer carcinogenesis was investigated by measuring quinone reductase (QR), glutathione S-transferase (GST) and 12-O-tetradecanoylphorbol-13-acetate(TPA)-induced ornithine decarboxylase (ODC) activities and glutathione (GSH) levels. Chitosan oligosaccharide induced QR activity in a dosedependent manner in a concentration range of 0.1~5.0 mg/mL. In addition GST activity was increased with chitosan oligosaccharide in cultured human colorectal adenocarcinoma HT-29 cells. The content of GSH was increased by the treatment of chitosan oligosaccharide. Chitosan oligosaccharide inhibited ODC activity, a key enzyme of polyamine biosynthesis. These results imply that low molecular weight chitosan oligosaccharide may suppress colon cancer carcinogenesis by increasing QR and GST activities and GSH content by inhibiting ODC activity.

에폭시수지의 주파수 변화에따른 트리잉 파괴 특성에 관한연구

우명하(Woo Myeong Ha),신진선(Shin Jin Sun),허준구(Heo Jun Gu),이지형(Lee Ji Hyung),박성철(Park Seong Cheol),조용우(Cho Yong Woo),정현목(Jeong Hyun Mok),윤찬영,신성식,박재준 대한전기학회 2015 대한전기학회 학술대회 논문집 Vol.2015 No.11

해양심층수와 키토산올리고당이 인간유방암세포의 혈관내피세포 성장인자 및 그 수용체의 발현에 미치는 영향

권윤숙 ( Yun Suk Kwon ),신진선 ( Jin Sun Shin ),이규식 ( Kyu Shik Lee ),남경수 ( Kyung Soo Nam ) 한국키틴키토산학회 2012 한국키틴키토산학회지 Vol.17 No.2

Vascular endothelial growth factor (VEGF) has been thought as a crucial regulator of angiogenesis for development, growth, proliferation, and metastasis of breast cancer. VEGF promotes angiogenesis by binding its receptor tyrosine kinases (RTKs), VEGFR-1 and -2. In this study, we evaluated the effect of deep-sea water (DSW) on expressions of chitosan oligosaccharide (COS-L)-mediated VEGF and its receptors induced by phorbol-12-myristate-13-acetate (TPA) in MDA-MB-231 breast cancer cells. We found that the mixtures of COS-L 1 mg/mL and DSW with various hardness have not shown cytotoxicity on MDAMB- 231 cells in MTT assay, and also VEGF expression induced by TPA was significantly decreased in a range of 0.05 to 3 mg/ mL of COS-L. DSW of more than hardness 400 enhanced the COS-L-mediated inhibition of VEGF expression. In the case of VEGFR-1 and R-2, DSW of more than hardness 200 and 400 significantly increased the COS-L-mediated inhibition of VEGFR expressions, respectively. These results indicate that DSW improves the inhibitory effect of COS-L on the expressions of VEGF and its receptors. Therefore using together DSW and COS-L is effective for prevention of breast cancer by down-regulating the expressions of VEGF and its receptors that leads to inhibiting angiogenesis.

저분자 키토산올리고당이 유방암 촉진효소에 미치는 영향

이규식 ( Kyu Shik Lee ),신진선 ( Jin Sun Shin ),남경수 ( Kyung Soo Nam ) 한국키틴키토산학회 2011 한국키틴키토산학회지 Vol.16 No.2

The effects of low molecular weight chitosan oligosaccharide (COS-L, MW<1,000 Da) on cancer promotion and metastasis in human breast cancer cell were investigated. In a concentration range of 0.05 to 3 mg/mL, cyclooxygenase-2 (COX-2), an important enzyme in prostaglandins biosynthesis from cellular arachidonic acids, and ornithine decarboxylase (ODC) protein expression were down-regulated in a dose-dependent manner. Transcription of aromatase which catalyzes the production of aromatic estrogen from androgen was also inhibited by COS-L. These results suggest that cancer promotion and metastasis of human breast cancer cell may be prevented by COS-L through down-regulation of COX-2 and ODC protein expression and aromatase transcription. Present investigation provides important information that COS-L should use as a cancer chemopreventive and therapeutic material in breast cancer.

Elevated Plus-maze를 이용한 Phenylpropanoid의 항불안 효과

윤병훈(Byung Hoon Yoon),최지웅(Ji Woong Choi),정지욱(Ji Wook Jung),신진선(Jin Sun Shin),현성예(Seong Ye Hyeon),정재훈(Jae Hoon Cheong),류종훈(Jong Hoon Ryu),고광호(Kwang Ho Ko) 대한약학회 2005 약학회지 Vol.49 No.5

The purpose of this study was to characterize the putative anxiolytic-like effects of phenylpropanoids using the elevated plus maze (EPM) test in mice. Cinnamic acid, p-coumaric acid, caffeic acid and ferulic acid were orally administered to male ICR mice, 1 h before behavioral evaluation in an EPM, respectively. Control mice were treated with an equal volume of vehicle, and positive control mice diazepam(1 mg/kg). A single treatment with phenylpropanoids (at 8 mg/kg) significantly increased time-spent and arm entries into the open arms of the EPM., and decreased time-spent and arm entries into the closed arms of the EPM versus control (P〈 0.05). However, no changes in the locomotor activity and myorelaxant effect were seen in any group versus the saline control. These results suggest that phenylpropanoids may be an effective anxiolytic agent.

저분자 키토산 올리고당의 유방암 발생 억제효과

남경수 ( Kyung Soo Nam ),이미현 ( Mi Hyun Lee ),이규식 ( Kyu Shik Lee ),신진선 ( Jin Sun Shin ),정상빈 ( Sang Bin Jeong ) 한국키틴키토산학회 2011 한국키틴키토산학회지 Vol.16 No.1

We investigated the effect of low molecular weight chitosan oligosaccharide (COS-L, Mw < 1,000) on breast cancer development and progression through the measurement of quinone reductase (QR) activity and glutathione (GSH) content and the determination of ornithine decarboxylase activity (ODC) induced by 12-O-tetradecanoylphorbol-13-acetate (TPA). In a concentration range of 0.1 to 5 mg/mL, QR activity and GSH content in MCF-7 breast cancer cells were significantly increased maximal 76.5% and 56.3% by COS-L in a dose-dependent manner, respectively. Low molecular weight chitosan oligosaccharide effectively inhibited the activity of ODC, a key enzyme in cancer promotion. These results demonstrate that COS-L may prevent the development and promotion of breast cancer through the induction of QR activity, the increase of GSH content and the inhibition of ODC activity.

2010년 국내 해수 중 영양염 분석 실험실간 상호비교실험 연구

문초롱,노태근,강동진,강성현,조성록,김은수,이정무,박은주,신진선,Moon, Cho-Rong,Rho, Taekeun,Kang, Dong-Jin,Kahng, Sung-Hyun,Cho, Sung Rok,Kim, Eun-Soo,Lee, Jung Moo,Park, Eun Ju,Shin, Jin-Sun 한국해양학회 2015 바다 Vol.20 No.1

2010년에 국내 10개 영양염 분석 실험실을 대상으로 실험실간 상호비교실험이 실시되었다. 균질성과 안정성이 확보된 영양염 농도가 각기 다른 3종의 멸균해수(Bottle 130, 131, 132)가 이용되었으며, 각 실험실은 아질산염(Nitrite), 질산염(Nitrate), 인산염(Phosphate), 규산염(Silicate)의 항목을 5회이상 반복 분석하여 결과를 회신하였다. 각 실험실의 측정정밀도와 실험실간 차이를 평가하기 위해서 분석결과를 통계처리 하였다. 대부분의 실험실이 모든 분석항목에서 10%이내의 측정정밀도를 보였고, 이상치를 제거한 실험실 간 차이는 모든 분석항목에서 4~63%의 상대표준편차를 보였다. 상대백분율차이는 0.04~2217%였다. 각 실험실에서 분석한 영양염 농도의 차이는 우연오차(random error)보다는 계통오차(systematic error)에 기인한다고 볼 수 있다. 균질성과 안정성이 확보된 시료 131을 이용하여 각 실험실의 분석결과를 보정한 결과, 보정 후 실험실 간 차이는 모든 분석항목에서 상대표준편차와 상대백분율차이가 현저히 향상되었다. 본 상호비교실험결과를 종합하면, 본 실험에 참가한 국내 영양염 분석 실험실 내 측정정밀도는 양호한 편이므로 적절한 기준물질을 사용하여 분석자료를 보정할 경우 실험실 간 차이가 줄어들어 다른 실험실에서 생산된 자료와의 자료 공유에 대한 수월성이 높아진다고 볼 수 있다. 따라서 영양염 시료 분석 시 균질성과 안정성이 확보된 표준물질을 사용하여 다른 실험실과의 비교가능성과 소급성을 확립할 것을 제안한다. An inter-laboratory comparison campaign on nutrient analysis in seawater was carried out in 2010. Sets of three sterilized seawater samples (Bottle 130, 131, 132) which have enough homogeneity and stability were distributed to 10 laboratories. Participants analyzed the nutrients in their own laboratories (nitrite, nitrate, phosphate, and silicate) at least 5 times and reported the results. Statistical treatments were applied to the results to assess the precision for each laboratory and the discrepancy among laboratories. Most laboratories show within 10% of precision in all nutrient results. Degrees of scattering described as discrepancy among laboratories and relative percent difference values were 4~63% and 0.04~2217%, respectively. The statistical analysis shows that the difference among the laboratories may due to the systematic error rather than random error. When the results were corrected by the results of bottle 130 as a reference material, the degrees of scattering and the relative percent difference were improved significantly. As a result, since most participants show satisfactory precision for nutrient analyses, a use of reference materials which have homogeneity and stability was strongly recommended to improve the comparability of nutrient data.

시화호 퇴적물에서 인의 지화학적 특성

심무준(Moo-Joon Shim),조성록(Sung-Rok Cho),나공태(Kong-Tae Na),신진선(Jin-Sun Shin),김은수(Eun-Soo Kim) 한국해양환경·에너지학회 2001 한국해양환경·에너지학회지 Vol.4 No.3