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염화 제 2 수은이 흰쥐 간장에서의 지질 과산화와 Oxygen Free Radical 제거 효소 활성도에 미치는 영향
신인철(In Chul Shin),고현철(Hyun Chul Koh) 한국응용약물학회 1994 Biomolecules & Therapeutics(구 응용약물학회지) Vol.2 No.3
Wistar albino rats were injected subcutaneously with mercuric chloride (5mg/kg) to define the early biochemical determinants that participate in the pathogenesis of mercuric chloride-induced hepatotoxicity, especially focusing on oxygen free radicals, we studied malondialdehyde(MDA) level and the activities of catalase and superoxide dismutase in the liver of rats at 24, 48 and 72 hr after the injection of mercuric chloride. MDA levels at 24, 48 and 72 hr after the injection of mercuric chloride increased as compared with that of control group. The activities of catalase and superoxide dismutase at 24, 48 and 72hr after the injection of mercuric chloride decreased as compared with that of control group. These results suggest that the depression of the activities of catalase and superoxide dismutase resulting from excessive oxygen free radicals is an important determinant in pathogenesis of mercuric chloride-induced hepatotoxicity.
일산화탄소 폭로후 고압산소 투여가 흰쥐 심장에서의 malondialdehyde 함량과 산소유리기 반응에 미치는 영향
신인철(In Chul Shin),고현철(Hyun Chul Koh),하지희(Ji Hee Ha) 한국응용약물학회 1998 Biomolecules & Therapeutics(구 응용약물학회지) Vol.6 No.1
In an attempt to define the effects of hyperbaric oxygen treatment on the lipid peroxidation and oxygen free radical reactions in rats exposed to carbon monoxide, we studied malondialdehyde(MDA) level and activities of catalase and superoxide dismutase in the heart of the rats exposed to carbon monoxide. Male Sprague-Dawley albino rats weighing 240 to 260gm were used. Experimental groups consist of Control group (=breathing with air), HBO group(=exposed to hyperbaric oxygen[HBO, 3ATA, 100%] after air breath), CO group(=exposed to CO[3,970 ppm] after air breath), CO-Air group(=exposed to CO after air breath followed by air breath) and CO-HBO group(=exposed to CO after air breath followed HBO treatment). The CO group showed significantly higher MDA level, catalase activity and SOD activity as compared to that of control group. The CO-HBO group showed significantly lower MDA level as compared to that of CO group, and did not show significantly lower catalase activity and SOD activity as compared to that of CO group. These results suggest that the excessive oxygen free radicals is an important determinant in pathogenesis of CO-induced cardiotoxicity and HBO inhibits the lipid peroxidation caused by excessive oxygen free radicals in the heart of the rats exposed to carbon monoxide.
신인철(In Chul Shin),고현철(Hyun Chul Koh) 대한약리학회 1996 대한약리학잡지 Vol.32 No.2
In an attempt to dofine the early biochemical determinants that participate in the pathogenesis of glycerol-induced nephrotoxicity, especially focusing on oxygen free radicals and N-acetyl-β-D-glucosaminidase(NAG) activity, we studied 24-hours urine outflow, 24-hours urinary protein excretion and urinary NAG activity after the injection of glycerol and also we studied malondialdehyde(MDA) level and superoxide dismutase(SOD) activity in the kidney of rats at 24hr after the injection of glycerol. Sprague-Dawley albino rats weighing 240 to 260 gm were injected intramuscularly with a 50% solution of glycerol(2ml/kg, 4ml/kg and 8ml/kg). The group treated with glycerol showed significantly lower urine outflow level and urinary protein excretion level and higher urinary NAG activity after the injection as compared to those of control group. Also the group treated with glycerol showed significantly higher MDA level and lower SOD activity at 24hr after the injection as compared to those of control group. These results suggest that the excessive oxygen free radicals resulting from the depression of SOD activity is an important determinant in the pathogenesis of glycerol-induced nephrotoxicity and higher urinary NAG activity is an index of renal tubular cell damage in the glycerol-induced nephrotoxicity.
Glycerol 이 흰쥐 신피질에서의 산소유리기반응과 신기능에 미치는 영향
신인철(In Chul Shin),고현철(Hyun Chul Koh) 한국응용약물학회 1995 Biomolecules & Therapeutics(구 응용약물학회지) Vol.3 No.4
In an attempt to define the early biochemical determinants that participate in the pathogenesis of glycerol-induced nephrotoxicity, especially focusing on oxygen free radicals, we studied malondialdehyde (MDA) level and the activities of catalase and superoxide dismutase in the renal cortex of rats, and the concentrations of blood urea nitrogen(BUN) and serum creatinine of rats at 24hr after the injection of a 50% solution of glycerol. Sprague-Dawley albino rats weighing 240 to 260 mg were injected intramuscularly with a 50% solution of glycerol(2 ㎖/㎏, 4 ㎖/㎏ and 8 ㎖/㎏). The group treated with glycerol showed significantly higher MDA level and eatalase activity, lower SOD activity and higher BUN and serum creatinine concentrations at 24 hr after the injection as compared to those of control group. These results suggest that the excessive oxygen free radicals resulting from the depression of SOD activity is an important determinant in the pathogenesis of glycerol-induced nephrotoxicity.
일산화탄소 폭로후 고압산소 투여가 흰쥐 신장에서의 malondialdehyde 함량과 catalase 및 superoxide dismutase 활성에 미치는 영향
신인철(In Chul Shin),강주섭(Ju Seop Kang),고현철(Hyun Chul Koh),하지희(Ji Hee Ha) 한국응용약물학회 1999 Biomolecules & Therapeutics(구 응용약물학회지) Vol.7 No.2
In an attempt to define the effects of hyperbaric oxygen treatment on the lipid peroxidation and oxygen free radical reactions in rats exposed to carbon monoxide, we studied malondialdehyde (MDA) level and activities of catalase and superoxide dismutase in the kidney of the rats exposed to carbon monoxide. Male Sprague-Dawley albino rats weighing 240 to 260 gm were used. Experimental groups consist of Control group (=breathing with air), HBO group (=exposed to hyperbaric oxygen [HBO, 3ATA, 100%] after air breath), CO group (=exposed to CO[3,970 ppm] after air breath), CO-Air group (=exposed to CO after air breath followed by air breath) and CO-HBO group (=exposed to CO after air breath followed HBO treatment). The CO group showed significantly higher MDA level, catalase activity and SOD activity as compared to that of control group. The CO-HBO group showed significantly lower MDA level as compared to that of CO group, and did not show significantly lower catalase activity and SOD activity as compared to that of CO group. These results suggest that the excessive oxygen free radicals is an important determinant in pathogenesis of CO-induced nephrotoxicity and HBO inhibits the lipid peroxidation caused by excessive oxygen free radicals in the kidney of the rats exposed to carbon monoxide.
신인철(In Chul Shin),고현철(Hyun Chul Koh) 한국응용약물학회 1995 Biomolecules & Therapeutics(구 응용약물학회지) Vol.3 No.3
In an attempt to define the effects of biphenyldimethyl dicarboxylate (DDB) on the lipid peroxidation and oxygen free radical scavenging enzymes activities in mercuric chloride-induced hepatotoxic rats, we studied malondialdehyde (MDA) level and the activities of catalase and superoxide dismutase (SOD) in liver of the rats at 24 hr after the injection of mercuric chloride. Sprague-Dalwey albino rats were injected subcutaneously with mercuric chloride (5 ㎎/㎏) only and mercuric chloride (5 ㎎/㎏) plus. DDB (200 ㎎/㎏/day, p.o) is administered for 4 days prior to 3 days from the injection of mercuric chloride. The group treated with mercuric chloride showed significantly higher MDA level and lower catalase and SOD activities as compared with that of control group. The group treated with mercuric chloride plus DDB showed significantly lower MDA level and catalase activity and higher SOD activity as compared with that of mercuric chloride-treated group. These results suggest that the excessive oxygen free radicals resulting from the depression of superoxide dismutase activity is an important determinant in the pathogenesis of mercuric chloride-induced hepatotoxicity and DDB has antioxidant effects.
알루미늄을 투여한 흰쥐의 해마와 대뇌피질에서 Reactive Oxygen Species 생성으로 인한 생체거대분자의 산화적 손상
문철진(Chul-Jin Moon),고현철(Hyun-Chul Koh),신인철(In-Chul Shin),이은희(Eun-Hee Lee),문해란(Hae-Ran Moon) 한국독성학회 2004 Toxicological Research Vol.20 No.3
This work aimed to study the effectiveness of cellular oxidative parameter (malondialdehyde,<br/> protein carbonyl, and 8-hydroxy-2'deoxyguanosine). The experimental groups were aluminum<br/> treated rats and control rats. Aluminum treatd rats were given intraperitoneally aluminum nitrate<br/> nonahydrate (Al3+, 0.2 mmol/kg) daily for 30 days except Sunday. Control rats were injected 1 ml of<br/> saline. After the dose, rats were decapitated and hippocampus and cerebral cortex were removed.<br/> The measured parameters were tissue malondialdehyde (MDA, index of lipid peroxidation), protein<br/> carbonyl (index of protein oxidation), 8-hydroxy-2'-deoxy-guanosine (8-OHdG, index of DNA oxidation),<br/> reduced glutathione (GSH) levels as well as glutathione reductase (GR) and catalase. Al concentrations<br/> in the tissues were also measured. All results were corrected by tissue protein levels. The<br/> results were as followed; 1. The concentrations of Al in the cortex and hippocampus were significantly<br/> higher in the Al-treated rats than in the control rats. 2. Antioxidative enzyme's activity, catalase<br/> and GR, were significantly higher in the Al-treated rats than the control rats. GSH levels were also<br/> higher in the Al-treated rats. 3. MDA, protein carbonyl, and 8-OHdG concentration of Al-treated rats<br/> were significantly higher than those of control rats. 4. The concentrations of antioxidants, and oxidative<br/> stress parameter were correlated with the concentrations of Al in hippocampus and cerebral cortex.<br/> Catalase and GR activity were also correlated with the concentration of Al. Based on these<br/> results, it can be suggested that intraperitoneally injected Al was accumulated in the brain and<br/> induced the increase of antioxidant levels and antioxidatve enzyme activity. Also, the oxidative products<br/> of cellular macromolecules are significantly related to tissue Al concentration. Therefore MDA,<br/> protein carbonyl, and 8-OHdG are useful markers for oxidative stress on cellular macromolecules.