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The purpose of this study is to investigate the effects of Carthami Flos on interstitial cells of Cajal in the gastrointestinal tract. Many regions of the tunica muscularis of the gastrointestinal (GI) tract display spontaneous contraction. These spontaneous contractions are mediated by periodic generation of electrical slow waves. Recent studies have shown that the interstitial cells of Cajal (ICCs) act as pacemakers and conductors of electrical slow waves in gastrointestinal smooth muscles. We investigated the cytotoxicity activity, antioxidant activity, and pacemaking activity. The cytotoxicity activity was measured by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay. Antioxidant activities were determined by DPPH (1.1-diphenyl-2-picrylhydrazyl) radical scavenging capacity assay and DCFH-DA (2,7-dichlorofluorescein diacetate) method. The effects of Carthami Flos on the pacemaker potentials in cultured ICCs from murine small intestine were investigated by using whole-cell patch-clamp techniques at 30℃. The addition of Carthami Flos (5, 10, 30 μg/ml) depolarized the resting membrane potentials in a concentration dependent manner. These results suggest that the GI tract can be targets for Carthami Flos, and their interaction can affect intestinal motility.
Objctive: To clarify the criterion, the characteristic of varaious age of ginseng radix cultivated in Korea and China were studied. Method: The surface of the transverse section of the specimen was made into a slid by the Paraffin Section method, and then dyed by Safranine Malachite Green method. The samples were observed at the power of 400 by an optic microscope(Olympus, Japan). The component and flavor of ginseng radix were analyzed by TLC(Thinlayer Chromatography) and electronic nose(FOX3000, France). Result: Ginseng radix according to the growing district and various age were comparative analyzed by optic microscope, TLC and electronic nose. The results were as followings. 1. The external form of Korean ginseng is longer and brightness then Chinese ginseng. 2. The internal form of Korean and Chinese ginseng are similar to each other. 3. The component of Korean and Chinese ginseng in TLC are similar to each other. 4. The fragrance of Korean and Chinese ginseng are clearly different. Conclusion: The results in this study demonstrate that morphology and component of Korean ginseng are similar to Chinese, on the other hand, fragrance of Korean and Chinese ginseng are different.
신준연 ( Joon Yeon Shin ), 최지원 ( Ji-won Choi ), 김동욱 ( Dong-uk Kim ), 주자기 ( Ziqi Zhou ), 권빛나 ( Bitna Kweon ), 배기상 ( Gi-sang Bae ), 송호준 ( Ho-joon Song ), 박성주 ( Sung-joo Park ) 대한본초학회 2021 大韓本草學會誌 Vol.36 No.1
Objectives : Forsythiae Fructus has been reported to have anti-inflammatory effects in many diseases, and Corydalis Tuber has been used as a pain suppressor in Eastern Asia. However, the protective effects of individual water extract of Forsythiae Fructus (FF) and Corydalis Tuber (CT) and the mixture of FF and CT (FC) on chronic pancreatitis (CP) were not well-investigated. Therefore, we investigated the protective effects of FF, CT, and FC on CP in mice. Methods : To induce CP, cerulein was injected 6 times a day, 4 times a week for 3 weeks. 1 h before the every cerulein injection, 200 ㎎/㎏ of FF, CT, or FC was intraperitoneally injected to mice. Histological analysis of pancreas was examined by hematoxylin and eosin stain and collagen deposition was examined by Masson's trichrome stain. Fibrogenic parameters such as α-smooth muscle actin (α-SMA), extracellular matrix (ECM) deposition, and fibrotic cytokines such as transforming growth factor (TGF)-β1 and platelet-derived growth factor (PDGF) were also analyzed by immunofluorescence stain and real-time PCR. Results : Histological damages in pancreas were inhibited by pre-treatment of FF or FC but not CT. α-SMA and ECM in pancreas were inhibited by pre-treatment of CT or FC but not FF. Moreover, the expression of TGF-β1 and PDGF in pancreas were inhibited by FF, CT or FC. Conclusions : Our results suggest that FC have protective effect on CP in mice through inhibition of α-SMA, ECM, TGF-β1 and PDGF in pancreas, and these findings could suggest new clinical strategy for CP.
Objective : The purpose of this study was to investigate the anti-cancer effects of Carthami Flos in some kinds of human colorectal adenocarcinoma cells. Method : We used two kinds of human colorectal adenocarcinoma cell lines, such as HT-29 and WiDr cells. We examined cell death by MTT assay and observed the morphological changes with Carthami Flos. Result : We showed that the combination of sub-optimal doses of Carthami Flos and cisplatin noticeably suppresses in HT-29 cells and doxorubicin in WiDr cells. Furthermore, we studied the caspase 3 activity to identify the apoptosis. Conclusion : Our findings provide insight into unraveling the effects of Carthami Flos in human colorectal adenocarcinoma cells and developing therapeutic agents against colorectal cancer.
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We studied the effects of the powders of Spirulina platensis (SPP) on anaphylactic reactions. SPP inhibited systemic anaphylaxis induced by compound 48/80 in rats. SPP also inhibited local anaphylaxis activated by anti-dinitrophenyl(DNP) IgE. Moreover, SPP dose-dependently inhibited histamine release in rat peritoneal mast cells activated by compound 48/80 or anti DNP-IgE. These results suggest that SPP may contain compounds with actions that inhibit mast cell degranulation in the rat.
00사는 예상되는 사이버공격 유형과 및 보호대상에 따라 각 지점별로 방화벽, 바이러스방역체계, 네트워크접근통제체계, 서버보안 솔루션, 자료유출방지체계 등 다양한 정보보호 장비들을 도입하여 운용 중에 있으나, 다수의 단위 지점을 운영하고 있는 00사의 여건(신규 도입 및 교체 시 소요 과다)과 현재 발전된 기술 성숙도(기존 단일 기능의 정보보호 솔루션들의 통합 제공 가능) 등을 고려할 때 효과적으로 체계를 구축하고 예산을 절감할 수 있는 통합솔루션 도입의 필요성이 식별되어, NFV(Network Function Virtualization) 기반 기술을 이용한 통합 사이버방호체계 개발안을 제시하고자 합니다.
Objectives : This study aimed to review the change in the origin of Sokdan(續斷) by diachronically analyzing literature data from Korea and China. Methods : Literature records describing the origin(synonyms, location of production, description) of Sokdan were collected, records were divided into periods. The main contents were included in the results, and original texts were edited and summarized in the table. Results : Sokdan, whose leaves resemble Jeoma(苧麻)(SJ), was first recorded in 《Xinxiubencao(新修本草)》, and described in detail in 《Bencaotujing(本草圖經)》 during the Tang and Song dynasties in China. In modern times, SJ was assumed to be genus Phlomoides or Lamium; however, records of the plant have decreased. Finally, SJ was not included in the 《Pharmacopoeia of the People's Republic of China》 as Sokdan. However, 《Diannanbencao(滇南本草)》, 《Diannanbencaotushuo (滇南本草圖說)》, 《Zhiwumingshitukao(植物名實圖考)》 described Sokdan of dian(滇). It was assumed genus Dipsacus . From the 1950s onwards, Sokdan is described in the literature as a member of the genus Dipsacus. In korea, SJ was recorded in 《Hyangyakjipseongbang(鄕藥集成方)》 and 《Dongeuibogam(東醫寶鑑)》 during the Joseon dynasty. In modern times, the genera Phlomoides and Lamium were mostly recorded as the origin of Sokdan. Conclusions : Several species have been described as Sokdan over the years in China, but since the 1950s, the genus Dipsacus was noted as the origin of Sokdan. In Korea, SJ was used in the past, thus Sokdan was recorded as P. umbrosa in 《The Korean Herbal Pharmacopoeia(KHP)ㆍ1985》. However, 《KHPㆍ1998》 referred to P. umbrosa as Hansokdan and D. asperoides as Sokdan.