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Glucose Oxidase가 척수지각신경절세포에 미치는 영향과 천마의 효과에 관한 연구
송호준,이용석,손영우,이강창,정종길,신민교,홍기연 대한본초학회 2002 大韓本草學會誌 Vol.17 No.2
Objectives : To clarify the cytotoxic effect of glucose oxidase(GO) and protective effect of gastrodiae Rhizoma (GR) on spinal sensory ganglion(DRG) neurons, neurotoxicity mediated by GO was measured by MTT assay and neurofilament enzymeimmuno assay(EIA). Methods : DRG neurons were cultured in the media containing various concentrations of GO for 3 hours. In addition, neuroprotective effects of GR, on GO-induced neurotoxicity in DRG neurons were examined after DRG neurons were preincubated with various concentrations of GR for 2 hours before 15mU/ml GO for 3 hours. Results : GO decreased remarkably cell viability in dependently in these cultures, and also RG increased cell viability and amount of neurofilament in DRG neurons damaged by GO. Conclusion : It is suggested that GO has toxic effect in cultured mouse DRG neurons, and also RG was effective in the protection of GO-induced neurotoxicity in these cultures.
한국산 독말풀에 대한연구(Ⅰ) : HPLC에 의한 Hyoscyamine 과 Scopolamine의 정량
도경삼,신민교,송호준,선중기,손인경,백승화 원광대학교기초자연과학연구소 1992 基礎科學硏究誌 Vol.11 No.3
A new method for quantitative determination of Hyoscyamine and Scopolamine in Datura stramonium L. by High Performance Liquid Chromatography(HPLC) was established. A reversed-phase system with a μ Bondapak C18 column using methanol in 0.2% aqueous phosphoric acid(40:60) with PIC A as a mobile phase was developed. Hyoscyamine and Scopolamine were detected at 254nm and the analysis was successfully carried out within 20min.
鹿蹄草 煎湯液 投與가 마우스의 免疫媒介 炎症反應 및 免疫細胞의 機能에 미치는 影響
손인경,노병규,변종호,송호준,신민교 대한본초학회 1998 大韓本草學會誌 Vol.13 No.1
This study was carried out to know the effects of Herba Pyrolae on the immune-inflammatio and the function or immunocytes of the mouse. To analyse the in vivo effects of Herba Pyrolae on the innate immunity, phagocytic ability and reactive oxygen intermediates(ROIs) formatio of the [eritoneal macropharges were measured, and in vivo specific immunities were analysed by measuring the antibody formatio against sheep red blood cells(SRBCs) and contact hypersensitivity reaction(CHR) against dinitrofluorobenzene(DNFB). The effects of Herba Pyrolae on the antibody forming cells were measured by the enumeration of rosette forming cells. The effects of Herba Pyrolae on the target cell killing were mersured by cytotoxicity of natural killer(NK)cells on the YAC-1 cells and of killer(K)cells in the antibody coated target cells. The in vivo effects of Herba Pyrolae adminstration on the immune system could be summerized as follows; 1. The adminstration of Herba Pyrolae enhanced the antibody dependent cellular cytotoxicity of K cells on the antibody-coated target cells. 2. The adminstration of Herba Pyrolae increased the tumor cell killing capacity of the NK cells. 3. The adminstration of Herba Pyrolae enhanced the phagocytic activity of peritoneal macrophages. 4. The adminstration of Herba Pyrolae decreased the CHR against DNFB slightly. 5. The adminstration of Herba Pyrolae enhanced the antibody (hemolysin and hemagglutinin) production against SRBCs. 6. The adminstration of Herba Pyrolae increased the rosette forming cells with SRBC in a dose dependent fashion. 7. The adminstration of Herba Pyrolae enhanced the capacity of phagocytes to produce ROIs slightly in a dose dependent fashion. The above enhancing effects of Herba Pyrolae adminstration on the function of immunocytes without affecting immuno mediated imflammation might be useful for the pervention and treatment of the various immune associated disorder.
이재규,이은미,오석규,손영우,유교상,김상수,이정헌,이강창,한두석,이승현,박승택,김요한,송호준 대한동의생리학회,대한동의병리학회 2001 동의생리병리학회지 Vol.15 No.6
In order to clarify the cytotoxic effect of cadmium in cultured oligodendrocytes of neonatal mouse, the cytotoxicity was measured by MTX assay in cultured cells treated with 1∼20uM CdCl_2 for 24 hours. And also, the protective effect of Achyranthis Bidentazae Radix(ABR) was examined by cell viability in these cultures. Cell viability was significantly decreased in a dose-and time-dependent manner after exposure of cultured oligodendrocytes to 10uM CdCl_2 for 24 hours. Protective effect of ABR on CdCl_2-mediated toxicity was very effective in these cultures. From above the results, it suggests that CdCl_2 is toxic in cultured oligodendrocytes and selective herb extract such as ABR is effective in protection of the CdCl_2 -induced cytoxicity.