전남지역에서 포획한 설치류의 한탄바이러스 감염에 대한 역학적 연구

송현제(Hyeon-Je Song),이대연(Dae-Yeon Lee),김충모(Choong-Mo Kim),신영학(Young-Hack Shin) 대한미생물학회 2006 Journal of Bacteriology and Virology Vol.36 No.3

전남지방에서 쯔쯔가무시병 숙주와 매개체의 서식환경 조사

송현제 ( Hyeon Je Song ) 한국동물위생학회 2012 韓國家畜衛生學會誌 Vol.35 No.3

Tsutsugamushi disease or scrub typhus cause by Orientia tsutsugamushi is an endemic disease in Korea. Chigger mites and field rodents play roles in transmission of the disease by the vector and host of the agent. The purpose of this study is to investigate the density of the chigger mites and field rodents due to environmental factors such as temperature, relative humidity, soil thickness and the various vegetations to the 9 field rodent collection sites. The total 62 field rodents was captured by the Sherman collapsible traps from April to October 2009 at the Jangseong of Jeonnam Province, Korea. The trapping rate of the field rodents by the different collecting sites was dominant at subside storage water (24%), bush near by dam (22%), bank around field (20%), followed by 18% of grassy field and surround cattle shed. The distribution of chigger mites by the different collecting sites was the highest at Bush near by dam (28.7%). And the sites of subside storage water, bank around field and surround cattle shed were 20.4%, 18.8%, 16.4%, respectively. On the other hand the collecting sites of stream bank and ridges between rice paddies were not collected. The temperature to the collecting sites was showed 24.1oC in June and 24.2oC in October which was higher than April (10.6oC), whereas lower than May (25.3oC) and September (26.8oC). The highest number of mites was collected at 24.2oC and 46.6% relative humidity in October. The chigger mites and field rodents were highly collected between 18 and 24% at the sites where are loosely in the superficial layers of the soil from 8.0 cm to 10.2 cm. Total 25 species of vegetation were distributed at the collecting sites. In the present study, strong evidence was found that bank around field and grassy field were provided for the prevalence sites of tsutsugamushi disease.

광주지역에서 분리된 장병원성대장균의 표현형 및 유전자형 특성에 관한 연구

김선희(Sun-Hee Kim),송현제(Hyeon-Je Song),정재근(Jae-keun Chung),하동룡(Dong-Rong Ha),류필열(Phil-Youl Ryu),이종빈(Jong-Bin Lee) 대한미생물학회 2006 Journal of Bacteriology and Virology Vol.36 No.3

모야모야 질병에서 관류 자기공명영상의 임상적 적용에 대한 연구

구은회 ( Eun Hoe Goo ),송현제 ( Hyeon Je Song ),동경래 ( Kyung Rae Dong ) 조선대학교 공학기술연구원 2014 공학기술논문지 Vol.7 No.2

The purpose of this study was to evaluate clinical application for patients with moyamoya disease by using perfusion MRI. The study targeted 22 moyamoya disease patients that included 9 men and 13 women in average age of 9.3 years and age range of 4~22 years. Perfusion MRI was obtained by scanning the 7.5cm part in the upward direction from the base of cerebellum before being processed for post-treatment, which led to acquisition of four map images for cerebral blood volume (CBV), cerebral blood flow (CBF), mean transit time (MTT) for contrast medium, and time to peak (TTP) for contrast medium. Lesions were examined in the CBV, CBF, MTT, and TTP maps of the perfusion MRI while the MTT and TTP were measured in the lesion parts and the normal and symmetric parts for comparison of differences. Perfusion defects were recognizable in all of the four maps of the perfusion MRI while delay in the MTT and TTP was conspicuous in the parts where perfusion defects were recognized. Therefore, perfusion MRI is useful for diagnosis and effective treatment for patient with moyamoya disease, which can be applied to clinical practice.

광주지역에서 발생한 바이러스성 설사질환의 역학적 연구(2000~2002: 2년간)

정재근(Jae-Keun Chung),송현제(Hyeon-Je Song),김선희(Sun-Hee Kim),서진종(Jin-Jong Seo),기혜영(Hye-young Kee),김은선(Eun-Sun Kim),하동룡(Dong-Rong Ha),류필열(Phil-Youl Ryu),이재일(Jaeil Lee) 대한미생물학회 2006 Journal of Bacteriology and Virology Vol.36 No.3

지리산둘레길에서 채집한 참진드기의 분포와 참진드기에서의 중증열성혈소판감소증후군바이러스 검출

송병준 ( Byung Joon Song ),임현철 ( Hyun Cheol Lim ),하태만 ( Tae Man Ha ),전두영 ( Doo Yung Jeon ),양수인 ( Soo In Yang ),송현제 ( Hyeon Je Song ) 한국가축위생학회 2016 韓國家畜衛生學會誌 Vol.39 No.2

Severe fever with thrombocytopenia syndrome (SFTS) is an emerging disease characterized by fever and thrombocytopenia. Haemaphysalis longicornis ticks comprise the major population of ticks in the environment and have been considered as the main vector for SFTS virus (SFTSV). Here we investigated the distribution of ticks carrying SFTSV collected from the environment using the dragging or sweeping methods during April∼October 2015 in Jeollanam-do, Korea. Sampling was taken from Songjeong, Omi, Bangkwang, Sandong areas in Jiri walking trails. Among the total 3,869 ticks collected, 3,823 ticks (98.8%) were H. longicornis, 41 (1.1%) were Amblyomma testudinarium, and 5 (0.1%) were Ixodes nipponensis. Classification results by regional groups of H. longicornis indicated that 1,613 ticks were collected in Sandong, 1,190 ticks in Omi, 603 ticks in Bangkwang, and 417 ticks in Songjeong. In monthly distributional studies of H. longicornis based on the developmental stages, nymph (325 ticks) was collected from May to October, 94% of larvae from April to June, and 94% of adult from June to August. These results showed the different dominant stage of ticks according to seasons. However, no SFTSV-specific gene was detected in 3,823 ticks of H. longicornis, 41 of A. testudinarium and 5 of I. nipponensis.

제주지역 4개 환경에서 채집한 중증열성혈소판감소증후군 매개 참진드기 분포

정경아 ( Kyoung A Chung ),송현제 ( Hyeon Je Song ),이혁재 ( Hyeok Jae Lee ),박철 ( Chul Park ),서민영 ( Min Yeung Seo ) 대한임상검사과학회 2020 대한임상검사과학회지(KJCLS) Vol.52 No.4

제주지역의 4개 환경(초지, 잡목림, 산길, 무덤)에서 2018년 3월부터 11월까지 진드기 채집을 통하여 진드기의 분포 특성 및 SFTSV 병원체 보균 여부를 조사하였다. Tick trap을 이용해 채집된 3,095마리와 flagging을 이용하여 채집된 1,569마리를 채집하였다. 총 4,664마리의 채집된 진드기 중에서 Haemaphysalis longicornis가 4,440마리(95.2%)로 대부분을 차지하였고, H. flava는 224마리(4.8%)가 채집되었다. 환경적으로는 Tick trap은 초지(953마리), 잡목림(883마리), 산길(847마리), 무덤(411마리)가 채집되었고, flagging은 잡목림(704마리), 산길(472마리), 무덤(197마리), 초지(196마리)가 채집되었다. 발육단계별로는 유충은 5월부터 채집되어 10월에 140마리, 11월에 280마리가 채집되었다. 약충은 4월부터 8월에 집중적으로 채집(2,978마리)되었고, 5월과 6월에 가장 많이 채집되었다. 성충은 6월부터 8월까지 전체 성충 중 94%가 채집되었다. 채집된 H. longicornis 4,440마리와 H. flava 224마리에서 SFTSV는 확인되지 않았다. The distribution of ticks and severe fever with thrombocytopenia syndrome virus (SFTSV) pathogens were investigated by collecting ticks from March to November 2018 in four environments (grass fields, copses, mountain roads, and tombs) in Jeju. Three thousand and ninety ticks were collected using a tick trap, and 1,569 ticks were collected using the flagging method. Of the 4,659 ticks collected, Haemaphysalis longicornis and Haemaphysalis flava accounted for 4,440 ticks (95.2%) and 219 ticks (4.7%), respectively. Nine hundred and fifty, 883, 847, and 410 ticks were collected from grass fields, copses, mountain roads, and tombs, respectively, using tick traps, whereas 704, 472, 197, and 196 ticks were collected from copses, mountain roads, tombs, and grass fields, respectively, using the flagging method. The largest fraction of ticks (2,978) was collected from April to August, and most were collected in May and June. Adult ticks comprised 94 percent of the total ticks from June to August. SFTSV was not detected in the 4,440 H. longicornis ticks or the 219 H. flava ticks collected in this study. Copyright Ⓒ 2020 The Korean Society for Clinical Laboratory Science. All rights reserved.

방사성 의약품 자동 분주장치의 유용성 및 최적화된 분주 방법 평가

이정은 ( Jeong Eun Lee ),김호성 ( Ho Sung Kim ),류재광 ( Jae Kwang Ryu ),동경래 ( Kyung Rae Dong ),송현제 ( Hyeon Je Song ),여화연 ( Hwa Yeon Yeo ) 조선대학교 공학기술연구원 2015 공학기술논문지 Vol.8 No.2

Recently, there is an increase of the number of hospitals using auto dispenser to reduce occupational radiation exposure when drawing up of the 18F-FDG dose (5.18 MBq/kg) in a syringe from the dramatic high activity of 18F-FDG multidose vial. The aim of this study is to confirm that using auto dispenser actually reduces the radiation exposure for technologists. Also we analyzed the reproducibility of auto dispenser to find optimized dispensing method for the device. We conducted three experiments. ① Comparison of radiation exposure on chest and hands: The chest and hands exposure dose received by technologists during the injection were measured by electronic personal dosimeter(EPD) and ring TLD respectively. ② Reproducibility of dispensed volume: We draw up the normal saline into 5 and 2 mL syringe using auto dispenser by changing the volume from 1 to 15 mm for 5 times in the same setting of the volume. The weight of 5 normal saline dispensed from the device at same volume was measured using micro balance and calculated standard deviation and coefficient of variation. ③Reproducibility of dispensed radioactivity: We dispensed 362.6 MBq ± 10% of 18F-FDG in 5 and 2 mL syringes from the multidose vial of different specific activity. In the same setting of volume, we repeated dispensing for 4 times and compared standard deviation and coefficient of variation of radioactivity between 5 syringes. There was significant difference in the average of chest exposure dose according to the dispensing methods(p < 0.05). Also, when dispensing 18F-FDG in manual method, exposure dose was 11.5 times higher in right hand and 4.8 times higher in left hand than in auto method. In the result of reproducibility of dispensed volume, standard deviation and coefficient of variation shows decline as the dispensing volume increases. As a result of reproducibility of dispensed radioactivity, standard deviation and coefficient of variation increases as the specific activity increases. We approved that the occupational radiation exposure dose of technologists were reduced when dispensing 18F-FDG using auto dose dispenser. Secondly, using small syringes helps to increase reproducibility of auto dose dispense. And also, if you lower the specific activity of 18F-FDG in multidose vial below 915-1020 MBq/mL, you can use auto dispenser more effectively keeping the coefficient of variation lower than 10%.

홍삼음료의 함량 및 안전성 실태조사

김종필(Jong-Pil Kim),김진희(Jin-Hee Kim),강경리(Gyung-Lee Gang),양용식(Yong-Shik Yang),홍삼재(Sam-Jai Hong),김은선(Eun-Sun Kim),문용운(Yong-Woon Moon),이정치(Jeong-Chi Lee),송현제(Hyeon-Je Song),정재근(Jae-Keun Chung) 한국식품과학회 2011 한국식품과학회지 Vol.43 No.4

대장균군은 유통형, 맞춤형 및 건강기능형 홍삼음료 모두에서 음성이었다. 일반세균은 유통형 홍삼음료 및 건강기능형 홍삼음료에서는 검출되지 않았으나, 맞춤형 홍삼음료에서는 58건 중 13건(22.4%)이 검출되었다. 총 100건의 제품 중 5건의 제품에서 안식향산이 검출되었다. 함량은 19.2-543.0 mg/kg이며 이 중 유통형 홍삼음료에서 검출된 1건의 제품(19.2 mg/kg)과 건강기능형 홍삼음료 1건(31.4 mg/kg)의 함량은 원료에서 유래된 것으로 판단되었다. 유통형 홍삼음료의 납 평균 함량은 62.3 μg/kg, 맞춤형 홍삼음료는 74.6 μg/kg, 건강기능형 홍삼음료는 28.8 μg/kg으로 나타났다. 카드뮴의 평균 함량은 유통형 1.15 μg/kg, 맞춤형 1.62 μg/kg, 건강기능형 홍삼음료는 4.18 μg/kg으로 조사되었다. 수은의 평균 함량은 0.10-0.18 μg/kg으로 조사되었다. 홍삼 건강기능식품의 지표성분인 Rg1과 Rb1함량은 유통형 및 맞춤형 홍삼음료가 0.1-23.4 mg/90 mL으로 함유되어 있었다. 하루에 3회 섭취한다고 가정하고 건강기능식품의 일일 섭취량 3-80 mg/day와 비교해보면, 이번에 조사된 37건의 유통형 홍삼음료 중에서 22건(59%), 맞춤형 홍삼음료 58건에서는 26건(45%)이 건강기능식품에 해당하는 지표성분(Rg1과 Rb1)을 함유하고 있는 것을 알 수 있었다. 그러나 홍삼음료들 간에 서로 홍삼성분의 함량차이가 크고 건강기능 식품 섭취기준(3-80 mg/day) 양에 미치지 못한 47건 중 1회 분량 90 mL 기준 홍삼성분이 0.2 mg 이하로 함유되어 있는 제품도 20 건이나 되는 것으로 나타났다. This survey was conducted to monitor the safety of red ginseng products in Gwangju, in 2010. Among 100samples, 37 were beverages, 5 were functional health foods on the market, and 58 were beverages from a tailor-made shop. All samples were negative for coliform bacteria. Aerobic plate counts were detected from 13 samples in the 58 tailor-made products but not detected in the other types of products. Benzoic acid was detected in 5 samples (range, 19.2-543.0 mg/kg). Among heavy metals, lead was detected, ranging from 28.8-62.3 μg/kg, cadmium, from 1.15-4.18 μg/kg, and mercury from 0.10-0.18 μg/kg. Benzopyrene was not detected in any samples. Ginsenoside Rg1 and Rb1 were detected in 0.1-23.4 mg/90 mL of beverages and 12.1-66.8 mg/90 mL of functional health foods. These results indicate that red ginseng products are safe in terms of microbial contaminants and hazardous chemical compounds such as heavy metals and benzopyrene.

Scrub Typhus : 확진된 76예의 임상 소견에 대한 전향적 연구

김동민,김현리,박치영,윤성호,송현제,심수경 대한감염학회 2006 감염과 화학요법 Vol.38 No.4

배경 : Orientia tsutsugamushi에 의한 인체 감염은 혈액과 임파선을 타고 전신에 퍼져 혈관 염을 일으켜, 전신 장기의 침범 소견을 보인다. 그러나 아직 까지 전신장기 침범에 대한 임상 소견 및 검사 결과의 전향적인 추적 관찰에 대한 연구는 거의 없는 실정이다. 재료 및 방법 : 조선대학교병원에 내원한 환자 중 확진법(gold standard)인 간접형광항체법(IFA)으로. 확인된 76명의 환자를 대상으로 내원시 임상 양상 및 검사 결과의 평가 및 치료 후 검사 결과의 변화에 대한 전향적인 연구를 수행하였다. 결과 : 대부분의 환자에서 발열, 갈증, 전신 쇠약감, 두통을 호소하였으며, 특이 할만한 소견으로 현재까지 보고되지 않았지만, 대부분의 환자가 갈증을 심하게 호소한다는 것이다. 3명의 젊은 건강한 환자를 제외한 대부분의 환자에서 이러한 갈증 증상을 호소하였으며, 이러한 갈증은 발열이 호전되어도 지속되었으며, 퇴원 이후에 까지 지속되는 양상을 보였다. 혈액검사상 scrub typhus 초기에 나타날 수 있는 신기능 장애 및 간 기능 장애는 대부분 가역적으로 적절한 항생제 투여 후 모두 정상으로 회복됨을 확인할 수 있었다. 혈액 검사상 CRP, LDH, AST가 대부분의 환자에서 상승하였고, 특히 LDH의 상승이100% 환자에서 관찰된 것은 특이할 만 한데 적절한 치료후 CRP는 신속히 호전을 보이나, LDH는 서서히 호전됨을 확인하였다. DIC 검사상 DIC는 입원환자의 95.5%에서 확인되었으나, 비교적 일부의 환자에서 출혈 및 경색이 초래되는 것으로 생각된다. 결론 : Scrub typhus 초기에 나타 날수 있는 신기능 장애, 간기능 장애 및 DIC는 대부분 가역적으로 적절한 항생제 투여 후 모두 정상으로 회복되며, 이러한 관찰이scrub typhus 환자의 진단 및 임상 경과의 이해에 도움이 되리라 사료된다. Background : Orientia tsutsugamushi spreads to the entire body through the blood and lymphatics, and it induces vasculitis that results in the patients manifesting symptoms of systemic organ involvement. Materials and Methods : We conducted a prospective study to evaluate the clinical manifestations and the change of the laboratory results after instituting treatment for scrub typhus. Results : Most patients presented with fever (100%), malaise (96.1%) and thirst (96.1%). It was remarkable that most patients presented with severe thirst, except for 3 healthy, young patients. This thirst was persistent even after the resolution of fever. The renal and hepatic dysfunction were reversible after the administration of appropriate antibiotics. For the blood testing, it was deteded that the CRP, and LDH were elevated in most patients (95.9% and 100% respectively). DIC could be diagnosed in 95.5% of the patients at the time of admission Conclusion : Most patients presented with fever, malaise and severe thirst, and the renal and hepatic dysfunction were reversible after the administration of appropriate antibiotics. DIC was observed in most of our patients, but hemorrhage and infarction were not present. CRP showed a rapid improvement, nonetheless, the LDH and DIC test results improved slowly.