AZCA 저항성 돌연변이 세포주로부터 선발 육성만 내염성 벼 돌연변이 계통의 특성 검정

송재영,김동섭,이긍주,이인석,강권규,윤성중,강시용,Song, Jae-Young,Kim, Dong-Sub,Lee, Geung-Joo,Lee, In-Sok,Kang, Kwon-Kyoo,Yun, Song-Joong,Kang, Si-Yong 한국식물생명공학회 2007 식물생명공학회지 Vol.34 No.1

본 연구는 벼 배 배양 캘러스에 방사선 조사와 AZCA 처리를 통해 AZCA저항성 세포주를 선발 육성하고 proline 함량이 증가된 선발 계통을 중심으로 염분 스트레스에 저항성을 갖는 벼 계통을 육성하고 그 기작을 밝히고자 하였다. 먼저, 1) AZCA 저항성 후대로부터 NaCl 저항성 식물체를 선발하고, 2) 선발된 저항성 계통의 생리적 생화학적 특성을 분석하였으며, 3) 분자적 특성을 RT-PCR을 통해 조사하고 유전적 변이를 탐색하였다. AZCA저항성 $M_{3}$ 3,000 계통으로부터 얻어진 약 20,000 종자에 염분 적정 선발 농도로 밝혀진 1.5%의 염분을 처리하여 내염성 (ST) 벼 116 개체를 선발하고, $M_{4}$ 후대 세대를 양성하였다. $ST\;M_{4}$ 세대에서 2차 내염성 계통 선발을 위해서 $M_{4}$ 세대계통을 1.2% NACl 에서 대조구와 생육 조사한 결과, 대조구 식물은 생육이 약하고 성장이 지연되는 것을 볼 수 있었다. 내염성 계통(ST-13, ST-16)으로부터 유도된 캘러스에 NaCl 처리한 결과, 대조구, ST-13, ST-16의 생존율은 9%, 16%, 20%로 나타났다. 또한, 필수 아미노산 함량을 잎, 종자 및 캘러스로 나누어 분석한 결과 ST-13와 ST-16는 대조구와 비교하여, 1) 잎에서는 약 1.24, 1.3배, 2) 종자에서는 1.49, 2.43배, 3) 캘러스에서는 1.32, 1.60배 증가하는 것이 확인되었다. 내염성 계통과 대조구에서 이온함량을 비교한 결과 잎과 뿌리에서 $K^{+},\;Na^{+}$ 및 $Na^{+}/K^{+}$ 비율을 보면 대조구보다 $Na^{+}/K^{+}$ 비율이 낮아진 것이 확인되었다. 내염성과 연관된 유전자 P5CS, NHXI를 이용하여 RT-PCR 실험을 수행한 결과, 돌연변이 계통에서 이들 유전자의 발현이 증가됨을 확인할 수 있었다. 본 연구에서 선발된 계통은 내염성 육종 및 기초 연구를 위한 재료로 이용될 수 있을 것으로 사료된다. To develop rice (Oryza sativa L.) cultivars to be planted on salt-affected sites, cell lines with enhanced proline content and resistance to growth inhibition by Azetidine-2-carboxylic acid (AZCA), a proline analogue, were screened out among calli irradiated with gamma ray of 50, 70, 90, and 120 Gy. The calli had been derived from embryo culture of the cultivar Donganbyeo. Selected AZCA resistant lines that had high proline accumulation were used as sources for selection of NaCl resistant lines. To determine an optimum concentration for selection of NaCl resistant lines, Donganbyeo seeds were initially cultured on the media containing various NaCl concentrations (0 to 2.5%) for 40 days, and 1.5% NaCl concentration was determined as the optimum concentration. One hundred sixteen salt-tolerant (ST) lines were selected from bulked 20,000 seeds of the AZCA resistant $M_{3}$ seeds in the medium containing 1.5% NaCl. The putative 33 lines ($M_{4}$ generation) considered with salt-tolerance were further analyzed for salt tolerance, amino acid and ion contents, and expression patterns of the salt tolerance-related genes. Out of the 33 lines, 7 lines were confirmed to have superior salt tolerance. Based on growth comparison of the entries, the selected mutant lines exhibited greater shoot length with average 1.5 times, root length with 1.3 times, root numbers with 1.1 times, and fresh weight with 1.5 times than control. Proline contents were increased maximum 20%, 100% and 20% in the leaf, seed and callus, respectively, of the selected lines. Compared to control, amino acid contents of the mutants were 24 to 29%, 49 to 143%, 32 to 60% higher in the leaf, seed and callus, respectively. The ratio of $Na^{+}/K^{+}$ for most of the ST-lines were lower than that of control, ranging from 1.0 to 3.8 for the leaf and 11.5 to 28.5 for the root, while the control had 3.5 and 32.9 in the leaf and root, respectively. The transcription patterns for the P5CS and NHXI genes observed by RT-PCR analysis indicated that these genes were actively expressed under salt stress. The selected mutants will be useful for the development of rice cultivar resistant to salt stress.

임베디드 시스템을 위한 가상 파일 시스템 구현

송재영(Jae-Young Song),이호송(Ho-Song Lee),성영락(Yeong Rak Seong),이철훈(Cheol-Hoon Lee),권택근(Taeck-Geun Kwon) 한국정보과학회 2003 한국정보과학회 학술발표논문집 Vol.30 No.1A

임베디드 시스템에 대한 관심과 개발이 급속도로 진행됨에 따라 임베디드 시스템에서도 파일 시스템을 필요로 하게 되었다. 특히 실 시간 운영체제 (Real Time Operating system : RTOS)를 사용하는 경우에는 운영체제가 사용되는 임베디드 시스템에 따라 필요로 하는 파일 시스템이 하나 또는 그 이상으로 다르게 되어 그 파일시스템을 통합하여 관리하기 위한 가상 파일 시스템 (Virtual File system : VFS)을 필요로 하게 되었다. 본 논문에서는 실 시간 운영체제와 함께 동작을 하는 가상 파일 시스템을 구현하는 방법을 제시한다.

산개구리류 4종(양서강; 개구리과)의 형태적 비교

송재영 ( Jae Young Song ),장민호 ( Min Ho Chang ),정규회 ( Kyu Hoi Chung ) 한국환경생물학회 2005 환경생물 : 환경생물학회지 Vol.23 No.2

한국산 산개구리류 3종의 분류학적 형질을 명확히 하기 위하여 형태학적, 계량형태학적 방법을 이용하여 한국산 산개구리류 3종(북방산개구리, 계곡산개구리. 아무르산개구리)과 일본산 산개구리류 1종 (R. tsuchimensis)을 비교분석하였다. 형태학적 비교 분석결과, 산개구리류는 손가락 끝과 발가락 끝의 모양, 아래턱에 형성된 반은의 발달유무, 윗입술선의 유무, 물갈퀴의 발달 정도, 서구개치열의 형태 등이 차이가 있는 것으로 확인되었으며, 계량형태학적 분석을 통하여 고막의 직경과 발길이의 차이를 확인할 수 있었다. 따라서, 이러한 형태학적, 그리고 계량형태학적 특징은 한국산 산개구리류를 동정하는데 매우 유용한 형질이라고 생각된다. To clarify the key characteristics of Korean brown frogs (R. dybowskii, R. huanrenensis and R. amurensis coreana), this study employed a morphological and morphometrical comparisons among brown frogs found in Korea and Japanese endemic species, R. tsushimensis. As a result, it was considered that the key characteristics were the shape of the finger and toe tips, the markings of the lower jaw, the presence or absence of an upper lip line, the development of toe webbing, and the shape of the vomerine teeth series by morphological comparisons of brown frogs. Also, there were significant differences in tympanum diameters and foot lengths of the four species of brown frogs. Therefore, a comparison between morphological and morphometric characters is an effective way to clarify the identification of brown frogs.

수술후 발생한 장피누공의 임상 고찰

송재영(Jae Young Song),유완식(Wan Sik Yu),서정욱(Jung Wook Suh) 대한외과학회 1985 Annals of Surgical Treatment and Research Vol.28 No.2

Purification and Properties of Thermostable L - Iactate Dehydrogenase Produced by Escherichia coli

송재영(Jae-Young Song),김경숙(Kyoug-Sook Kim) 한국식품영양과학회 1994 한국식품영양과학회지 Vol.23 No.6

Bacillus stearothermophilus의 유산탈수소 효소에 대한 4.3kb인 유전자를 subcloning하여 대장균에서 생산된 효소의 특성을 조사하였다. 본 효소는 25%의 수율로 200배 정제되었으며, 열처리, OEAE-Sephadex A-50, NAD^+ -Sepharose CL-4B affinity chromatography, gel filteration을 거쳐서 정제되었다. Gel filteration에 의해 정제된 효소의 분자량은 약 140,000이었으며, SDS-polyacrylamide 전기영동 결과, 분자량 35,000의 단일 band를 나타내어 4개의 subunit로 구성된 효소임을 알 수 있었다. 정제효소에 대한 pyruvate 환원 반응과 lactate 산화 반응에 있어서의 최적 pH는 각각 5.5와 8.5이었고, pH 안정성은 pyruvate 환원 반응에 있어서 5에서 7 사이였다. 효소활성에 대한 최적온도 및 열안정성은 pyruvate 환원 반응에서 60℃, lactate 산화 반응에서 70℃와 75℃이었다. 본 효소는 FeSO4와 pCMB에 의해 강한 저해 반응을 나타내었으며, sodium oxalate는 경쟁적 저해제로 작용하였다. 또한 fructose 1,6-bisphosphate에 의한 효소활성 촉진은 0.8mM에서 최대의 활성을 나타내었다. The 4.3-kb gene coding for L-Iactate dehydrogenase of Bacillus stearothermophilus has been subcloned and expressed in E. coli cells. The enzyme was purified 200-fold with 25% yield by heat treatment, DEAE-Sephadex, and NAD^+ -Sepharose CL-4B affinity chromatography followed by gel filtration through Sephadex G-200. The molecular weight of the purified enzyme was estimated to be about 35,000 and 140,000 on SDS-polyacrylamide gel electrophoresis and gel filtration, respectively, indicating that the enzyme is composed of four identical subunits. The enzyme for pyruvate reduction and lactate oxidation was stable at 60 and 75℃ for 30min, and the optimal temperatures for both reactions were 60 and 70℃, respectively. The enzyme had an optimal pH at 5.5 and 8.5 in pyruvate reduction and lactate oxidation, respectively. The pH stability of enzyme for pyruvate reduction was stable between pH 5 and 7. More than 90% of enzyme activity was lost at 1mM FeSO₄ and p-chloromercuribonzoate. The maximal activation of the enzyme was obtained with 0.8mM fructose 1,6-bisphosphate.

DEI 달성을 위한 초급 한국어 디지털 교과서 구축 방안

송재영 ( Song Jae Young ) 국제한국어교육학회 2022 한국어 교육 Vol.33 No.4

The purpose of this study is to improve learners’ Korean language skills through diversity, equity, and inclusion (DEI). This study examines how DEI could be achieved by developing a novel digital textbook for the beginning level of Korean classes at Wellesley College. First, inclusiveness was accomplished by setting the Wellesley students as the main characters and using the main living spaces of Wellesley students as the background. When establishing the main characters, diversity was expanded by considering race, gender identity, disability, and various learning goals. To promote equity, the textbook was built as a digital form and provided free of charge, so that learners could easily use various learning tools regardless of their geographic location or socioeconomic status. In the process of transitioning to in-person classes, I discuss ways to utilize digital textbooks in offline classes and seek directions for future development through feedback from learners. (Wellesley College)

제대기저부 탈장

송재영(Jae Young Song),김상윤(Sang Youn Kim),서정욱(Jung Wook Suh) 대한외과학회 1985 Annals of Surgical Treatment and Research Vol.28 No.1

DAMR-PCR 마커를 이용한 피 ( Echinochloa crus-galli) 유전자원 다양성 분석

송재영 ( Jae Young Song ),박기훈 ( Ki Hun Park ),조광민 ( Kwang Min Cho ),현도윤 ( Do Yoon Hyun ),이수경 ( Sukyeung Lee ),최유미 ( Yu Mi Choi ),박홍재 ( Hong Jae Park ),이석영 ( Sok Young Lee ),이명철 ( Myung Chul Lee ) 전북대학교 농업과학기술연구소 2014 농업생명과학연구 Vol.45 No.2

This study evaluated the effectiveness of directed amplification of minisatellite-region DNA (DAMD) markers in assessing polymorphism and relationships of 69 Echinochloa crus-gali accessions that preserved in the National Agrobiodiversity Center. Using these primers, a total of 113 bands were amplified, of which 65 polymorphic bands were discovered and the percentage of polymorphic bands (PPB) was 57.52%. SPAR profile data were scored for the computation of pairwise distances as well as a Neighbor Joining (NJ) tree of all the genotypes. From the profiles obtained with all the 12 primers considered together, 65 polymorphism bands were scored. The averaged values of gene diversity (HE) and polymorphism information content (PIC) for each DAMD marker were 0.213 and 0.179 within populations, respectively. The results indicate the low levels of the genetic diversity present amongst the genotypes. The 69 Echinochloa crus-galli germplasm accessions were divided into four different groups, and data analysis identified phenetic groups that were in agreement with those obtained according to agronomic traits and DAMD markers. The present results provide evidence of divergence between cultured and wild type barnyard grass. The cultured barnyard grass was seven accessions that had been cultivated in Korea and have been cultivating in India among the 69 Echinochloa crus-gali accessions. The selected seven accessions have thicker culm diameter and bigger seed size than the other groups, and is one of the putative germplasm resources to develop the new forage crop and phytoremediation.

DEA 모형을 이용한 세계 주요 항만의 효율성 평가

송재영(Jae-Young Song),신창훈(Chang-Hoon Sin) 한국항해항만학회 2005 한국항해항만학회지 Vol.29 No.3

본 연구는 부산항을 포함한 세계 주요 컨테이너 항만들의 효율성을 DEA(Data Envelopment Analysis)모형을 통해 상대적으로 분석함으로써, 부산항의 현재 위치와 더 효율적인 항만이 되기 위해 벤치마킹(Benchmarking) 해야 할 대상을 구체화 하였다. 또한, 본 연구에서는 일정시점의 효율성 분석이 아닌 1995년~2001년까지 7개년의 효율성을 시계열적으로 분석함으로써 각 항만의 효율성 변화를 살펴보았다. 분석 결과, 부산항은 95년, 96년을 제외한 나머지 연도에 대해서 비효율적인 항만으로 구분되었으며, 97년 이래로 효율성 지수가 다소 증가하고 있는 것으로 나타났다. This paper presents the measurement of efficiency for container ports. Data envelopment analysis(DEA), as it is called, has particular applicability in the service sector. Applying mathematical programming techniques, DEA enables relative efficiency ratings to be derived within a set of analysed units. This paper investigates the efficiency employing DEA Model on data for 53 container ports covering 1995~2001 in the world and the change in efficiency for 7 years. As a results, port of Busan was evaluated as inefficiency port compare with major ports of the world except 1995year and 1996year. But After 1997year, efficiency of Busan port is increasing somewhat better every year.

축제 방문동기에 따른 시장세분화연구 -김제지평선축제를 중심으로-

송재영 ( Jae Young Song ),정강환 ( Gang Hoan Jeong ),호정 ( Ting Hu ) 한국관광산업학회 2013 Tourism Research Vol.38 No.2

Festival visitors market segmentation can help festival managers to understand visitors` needs ,and meet them based on their different characteristics. While the studies on the market segmentation for goods are being done, those for festival visitors are lack now. Therefore, the purpose of this study is to segment festival visitors according to their festival visit motivations. In ordre to achieve the purpose of the study, the survey was conducted towards festival visitors who attended the Gimje Horizon Festival. 260 effective samples were collected and used in the final analysis. As a result, it is found that according to visit motivations three groups in terms of escapism experience-oriented group, passively motivated group, and actively motivated group were derived from the analysis. Significant differences were also found among the groups according to their demographic characteristics. Based on the results, strategic implications were proposed for festival managers. Different service should be provided for each market. The differentiated program development is needed for each market. At the same time the concrete promotion marketing strategy has to be established for each market.