Characterization of PCR fragment of metallothionein gene from liver mRNS of channel catfish

송영환,Song, Young-Hwan 한국어병학회 1997 한국어병학회지 Vol.10 No.1

Metallothionein은 세균에서 척추동물에 이르기 까지 모든 생명체에 존재하며, 중금속의 세포내농도를 조절하는 중요한 단백질이다. 현재까지 metallothionein의 기능 및 유도기작에 관한 연구는 많이 진척되지는 않았으나, 여러 metallothionein 유전자의 구조가 밝혀져 있는 실정이다. 특히 어류의 metallothionein은 여러종류의 중금속과 환경적인 자극에 의하여 유도되고 정량적인 RT-PCR의 방법으로 metallothionein 유전자의 RNA transcript를 측정함으로써 환경적인 자극의 정도와 중금 속의 상대적인 양을 측정할 수 있기 때문에 중요한 단백질로 인식되고 있다. 본 연구에서는 유전자내부의 특이적 primer와 통상적인 3`말단의 primer를 이용하여 PCR에 의해 450 bp에 해당하는 metallothionein 유전자의 일부의 특성을 조사하였다. 챠넬메기의 cDNA library로부터 PCR에 의해 증폭된 450 bp의 PCR 절편은 다른 어류의 metallothionein 유전자와는 유사성을 보이지 않았다. Metallothionein is an important and essential protein to control the intracellular concentration of heavy metals, which exist in all organisms from bacteria to vertebrates. Although the detailed functions and induction mechanisms of metallothionein gene have not been clearly characterized until yet, the structure of several metallothionein genes has been revealed. Especially, piscine metallothionein is regarded as an important protein because it is induced by several heavy metal pollutants and environmental stress and it could be determined the comparative amount of heavy metals and the extent of environmental stress by assaying the RNA transcript of metallothionein gene in the method of the quantitative RT-PCR(Reverse Transcriptase Polymerase Chain Reaction). In this study I have characterized the 450 bp PCR fragment of metallothionein gene amplified by using the mixture of internal specific primers and universal 3' end primer. The nucleotide sequence analysis of 450 bp PCR fragment amplified in cDNA library of channel catfish did not show strong homology to other piscine metallothionein genes.

연속음성신호의 SNR 추정기법에 관한 연구

송영환,박형우,배명진,Song, Young-Hwan,Park, Hyung-Woo,Bae, Myung-Jin 한국음향학회 2009 韓國音響學會誌 Vol.28 No.4

음성 신호처리 환경에서 잡음이 섞인 신호를 개선할 목적으로 음성향상 기법이 많이 이용되고 있다. 잡음추정 알고리즘은 변화하는 환경에 빠르게 적응할 수 있어야 하며 음성신호의 영향을 줄이기 위해 음성신호가 존재하지 않는 구간에서만 잡음의 파워를 갱신한다. 이러한 방법은 음성구간검출이 선행되어야 한다. 그러나 잡음에 열화된 음성신호에 묵음구간이 존재하지 않을 경우, 위와 같이 음성검출을 통한 묵음구간에서의 잡음 추정 방법 및 SNR 추정 방법이 적용될 수 없다. 본 논문에서는 묵음구간이 존재하지 않는 연속음성신호에서 SNR을 추정하는 기법을 제안한다. 유성음의 안정구간에서는 단구간 내 피치의 변화가 매우 작아 피치주기에 따른 음성신호의 파형이 유사하게 나타난다. 따라서 잡음이 음성에 부가되었을 때 피치주기에 따른 인접파형의 유사도를 통해 SNR을 추정한다. 무성음에서는 잡음의 영향이 수신신호의 성도성분 추정에 영향을 미치기 때문에 잡음환경에서 추정된 성도성분과 수신신호 스펙트럼 간의 거리를 이용하여 SNR을 추정한다. 마지막으로, 음성신호의 에너지가 유성음에 대부분 분포하기 때문에, 부가성 잡음 환경에서 유성음의 에너지를 음성신호의 에너지로 근사화하여 SNR을 추정할 수 있다. In speech signal processing, speech signal corrupted by noise should be enhanced to improve quality. Usually noise estimation methods need flexibility for variable environment. Noise profile is renewed on silence region to avoid effects of speech properties. So we have to preprocess finding voice region before noise estimation. However, if received signal does not have silence region, we cannot apply that method. In this paper, we proposed SNR estimation method for continuous speech signal. The waveform which is stationary region of voiced speech is very correlated by pitch period. So we can estimate the SNR by correlation of near waveform after dividing a frame for each pitch. For unvoiced speech signal, vocal track characteristic is reflected by noise, so we can estimate SNR by using spectral distance between spectrum of received signal and estimated vocal track. Lastly, energy of speech signal is mostly distributed on voiced region, so we can estimate SNR by the ratio of voiced region energy to unvoiced.

일본어 합성기에서 유동 Break를 이용한 합성단위 선택 방법

송영환,나덕수,김종국,배명진,이종석,Song, Young-Hwan,Na, Deok-Su,Kim, Jong-Kuk,Bae, Myung-Jin,Lee, Jong-Seok 한국음향학회 2007 韓國音響學會誌 Vol.26 No.8

대용량 코퍼스를 이용하는 합성단위 선택(unit selection) 기반 합성기에서 break는 자연성 및 명료성에 큰 영향을 미치는 파라미터로 unit selection 과정에서 음소 정보와 함께 중요한 특징으로 사용된다. 일본어는 피치의 상대적 높낮이로 표현되는 악센트를 가지는 언어이고, 악센트의 변화에 따라 AP(Accentual Phrase)가 결정되고 AP 경계에서 break가 형성된다. break는 규칙 기반 방식이나 통계적 방식인 J-ToBI를 이용하여 예측 할 수 있으나 다양성으로 인해 정확한 예측이 어렵다. 따라서 본 논문에서는 다양한 운율 정보를 포함하고 있는 대용량 코퍼스의 장점을 이용하기 위해 break를 고정 break와 유동 break로 나누어 합성단위 검색을 수행한다. 실험 결과 제안한 합성단위 선택 방법으로 합성음의 자연성을 향상 시킬 수 있었다. In a large corpus-based speech synthesizer, a break, which is a parameter influencing the naturalness and intelligibility, is used as an important feature during a unit selection process. Japanese is a language having intonations, which ate indicated by the relative differences in pitch heights and the APs(Accentual Phrases) are placed according to the changes of the accents while a break occurs on a boundary of the APs. Although a break can be predicted by using J-ToBI(Japanese-Tones and Break Indices), which is a rule-based or statistical approach, it is very difficult to predict a break exactly due to the flexibility. Therefore, in this paper, a method is to conduct a unit search by dividing breaks into two types, such as a fixed break and a flexible break, in order to use the advantages of a large-scale corpus, which includes various types of prosodies. As a result of an experiment, the proposed unit selection method contributed itself to enhance the naturalness of synthesized speeches.

도축돈 장분변으로부터 Shiga Toxin-Producing Escherchia coli의 분리 와 성상

송영환,김지영,채미경,박창식,김명철,전무형,Song, Young-hwan,Kim, Ji-young,Chae, Mi-kyung,Park, Chang-sik,Kim, Myung-chul,Jun, Moo-hyung 대한수의학회 2004 大韓獸醫學會誌 Vol.44 No.4

Shiga toxin-producing Escherichia coli (STEC) causes various clinical signs in human and animals, and has been indicated as a global enteropathogen with zoonotic importance. In this study, the feces of healthy pigs were collected from the slaughtered pigs of Daejon abattoir during the period from December 2001 to October 2002. Of 326 specimens, 13 STEC were confirmed by culture, PCR and colony hybridization. The isolates were further studied for toxin types, pathogenic factors, plasmid profiles, and antimicrobial resistance to characterize the genetic and toxigenic properties. In PCR, all of 13 isolates were evident to have shiga toxin gene (stx). Of 13 isolates stx1 gene was detected in 4 and stx2 gene in 9. The genes of eaeA, hlyA and rfbE were not present in any isolates. In colony hybridization using shiga toxin common primer (STXc), 2 to 9 per 100 colonies subcultured from 13 isolates showed the positive reaction. In the examination for plasmid profiles of the isolates, one to eleven plasmids with varying sizes of 1.0 Kb to 100 Kb were detected, and the 13 STEC could be classified into four groups by the plasmid patterns. The antimicrobial resistance patterns of the isolates were comparably corresponded with the plasmid profile patterns.

수정 대혈관 전위 환자에서 예후에 영향을 주는 인자들에 대한 중기적 고찰

송영환,권혁주,김기범,강수정,배은정,노정일,윤용수,이정렬,김용진,노준량,Song, Young-Hwan,Kwon, Hyok-Joo,Kim, Gi-Beom,Kang, Soo-Jung,Bae, Eun-Jung,Noh, Chung-Il,Yun, Yong-Soo,Lee, Jeong-Ryul,Kim, Yong-Jin,Rho, Joon-Ryang 대한소아청소년과학회 2003 Clinical and Experimental Pediatrics (CEP) Vol.46 No.2

목 적 : 수정 대혈관 전위 환자에서는 동반 심장 기형, 우심실 기능, 삼첨판 판막 기능, 부정맥 등이 예후에 영향을 주는 것으로 알려져 있다. 이 연구에서는 중기간의 관찰을 통해 여러 예후 인자들을 비교 분석하였다. 방 법 : 서울대학교병원에서 C-TGA로 진단 받은 94명을 대상으로 후향적 임상 기록 고찰을 시행하였다. 대상 환자들은 남아가 58명, 여아가 36명이었고, 추적 관찰 시 최종 평균 연령은 $12{\pm}9$세였으며, 평균 추적 관찰 기간은 $9{\pm}6.4$년이었다. 결 과 : 대상 환자 94명 중에 심장 수술은 72명이 시행 받았고, 고전적 교정술이 55례, 해부학적 교정술이 17례였다. 동반된 심장 기형(삼첨판 역류, 폐동맥 협착이나 폐쇄, 심실 중격 결손, 임상군), 심장 수술 여부와 종류, 완전 방실 전도 차단을 예후인자로 설정하였고, 예후 인자들 중에서 삼첨판 역류, 폐동맥 협착, 심실 중격 결손은 중등도 이상인 경우만 양성으로 채택하였다. 본 연구에서는 삼첨판 역류만이 생존율에 영향을 주는 예후인자였으며, Ebstein 기형과 고도 방실 전도 차단이 삼첨판 역류와 연관성이 있었다. 삼첨판 부전이 있는 환자의 20년 생존율은 35%로, 없는 환자 77%에 비교해서 유의한 차이를 보였고, 수술 직후 사망을 제외한 소그룹 분석에서도 삼첨판 부전이 있는 경우와 없는 경우의 20년 생존율이 각각 48%와 87%로 유의한 차이를 보였다. 20년 생존율 분석과 삼첨판 역류와의 연관성 분석에서 고전적 교정술과 해부학적 교정술간에 유의한 차이는 없었다. 결 론 : 삼첨판 부전은 수정 대혈관 전위 환자의 중요한 예후인자이며, Ebstein 기형과 방실 전도 장애가 삼첨판 부전에 영향을 줄 수 있다. 이 밖에 심장 수술, 동반 심기형 등 다른 예후인자에 대한 정확한 평가를 위해서는 장기적인 추적 관찰이 필요하다. Purpose : The prognosis of patients with corrected transposition of the great arteries(C-TGA) is variably affected by associated intracardiac defects, systemic right ventricular function, tricuspid valve competence, and conduction disturbances. This study aims to evaluate the importance of those factors at mid-term follow-up. Methods : Medical records of 94 patients(males 58, females 36; mean age at last follow-up, $12{\pm}9$ years; mean follow-up duration, $9{\pm}6.4$ years) diagnosed between January 1980 and May 2002 at Seoul National University Children's Hospital were studied retrospectively. Results : Among 94 patients, operations were performed in 72 patients(classic operations in 55; double switch operations in 17). Among prognostic factors including associated intracardiac anomalies(at least moderately severe tricuspid insufficiency(TI), ventricular septal defect, pulmonary stenosis and pulmonary atresia), intracardiac operation and complete atrioventricular block, TI was the only significant factor for death(P=0.001), and in turn, Ebstein anomaly and high grade atrioventricular block predicted TI. 20-year survival without TI was 77%, but only 35% with TI(P=0.0002); excluding perioperative death, the 20-year survival rates with and without TI were 48% and 87% respectively(P=0.008). There was no statistical difference in 20-year survival rate or association with TI between classic and double switch operation. Conclusion : TI was the major prognostic factor for C-TGA and was associated with Ebstein anomaly and high grade atrioventricular block at mid-term follow-up. Long-term follow-up is required to evaluate other factors, including double switch operations and associated intracardiac defects more exactly.

효모의 ADE1 유전자의 Cloning 에 대하여

송영환,서연수,강현삼 ( Young Hwan Song,Yeon Soo Seo,Hyen Sam Kang ) 생화학분자생물학회 1984 BMB Reports Vol.17 No.4

YEp24, Yeast-E. coli shuttle vector, carry the URA3 gene as a selection marker and contain the replication origin of 2 ㎛ plasmid which is the endogenous plasmid in Saccharomyces cerevisiae and have two cloning sites i.e. BamHI and SalI. We prepared the total gene bank of Saccharomyces cerevisiae by inserting the Sau3A1 partial digested fragments which was size-fractionated into the BamHI site of the YEp 24. The hybrid plasmid pool were isolated from the total gene bank of Saccharomyces cerevisiae to clone the ADEI gene and by using the SHY 3 as yeast host which carry the adel-101 and ura3-52 marker, we could isolate the yeast transformants carried the ADEI gene on synthetic minimal media dificient Adenine and Uracil by complementation. We determined the restriction map and observed the stability of the recombinant plasmids of three transformants among 15 transformants. We observed the close similarity of the two recombinant plasmids in the restriction pattern and the stable maintenance of the recombinant plasmids in non-selective condition.

증착 후 진공열처리에 따른 SnO₂ 박막의 특성 변화

송영환,문현주,김대일,Song, Young-Hwan,Moon, Hyun-Joo,Kim, Daeil 한국열처리공학회 2016 熱處理工學會誌 Vol.29 No.4

$SnO_2$ thin films were prepared by radio frequency magnetron sputtering on glass substrates and then vacuum annealed for 30 minutes at 100, 200, and $300^{\circ}C$, respectively. The thickness of films kept at 100 nm by controlling the deposition rate. While the optical transmittance and electrical resistivity of as deposited $SnO_2$ films were 82.6% in the visible wavelength region and $1.9{\times}10^{-3}{\Omega}cm$, respectively, the films annealed at $200^{\circ}C$ show the increased optical transmittance of 84.5% and the electrical resistivity also decreased as low as $8.5{\times}10^{-4}{\Omega}cm$. From the observed results, it is concluded that post-deposition vacuum annealing at $200^{\circ}C$ is an attractive condition to optimize the opto-elecrtical properties of $SnO_2$ thin films for the opto-electrical applications.

원유분해세균의 분리 및 특성

송영환(Young Hwan Song) 한국어병학회 1992 한국어병학회지 Vol.5 No.2

From several sites of petroleum storage basement in South Coasts in Korea, various petroleum degrading bacteria have been isolated and characterized as Pseudomonas fluorescens, Acinetobacter baumanii, Pseudomonas maltophila and Pseudomonas aeruginosa, respectively. They show the ability of petroleum degradation on minimal media which contains petroleum as sole carbon source and loose the ability at high concentration of NaCl as increasing the concentration of NaCl from 0.5% to 6% . It has been confirmed that such bacteria have utilized the simple saturate hydrocarbon; n-decane, n-hexane, n-octane and n-decane because petroleum consists of various kinds of organic compounds. It has been also identified that petroleum degrading bacteria habor the plasmid and show the antibiotic resistance against ampicillin, tetracycline and chloramphenicol. These results strongly suggest that the petroleum degrading gene and antibiotic resistance gene might be located on the high molecular weight plasmid.

Ag 성막위치에 따른 ZTO/폴리카보네이트 필름의 특성 변화

송영환 ( Young-hwan Song ),엄태영 ( Tae-young Eom ),천주용 ( Joo-yong Cheon ),차병철 ( Byung-chul Cha ),최동혁 ( Dong-hyuk Choi ),손동일 ( Dong-il Son ),김대일 ( Daeil Kim ) 한국열처리공학회 2017 熱處理工學會誌 Vol.30 No.3

100 nm thick Sn doped ZnO (ZTO) single layer, 15 nm thick Ag buffered ZTO (ZTO/Ag), Ag interme-diated ZTO (ZTO/Ag/ZTO) and Ag capped ZTO (Ag/ZTO) films were prepared on poly-carbonate (PC) sub-strates by RF and DC magnetron sputtering and then the influence of the Ag thin film on the optical and electrical properties of ZTO films were investigated. As deposited ZTO thin films show the visible transmittance of 81.8%, while ZTO/Ag/ZTO trilayer films show a higher visible transmittance of 82.5% in this study. From the observed results, it can be concluded that the 15 nm thick Ag interlayer enhances the opto-electrical performance of ZTO thin films effectively for use as flexible transparent conducting oxides films in various opto-electrical applications. (Received April 7, 2017; Revised April 14, 2017; Accepted April 20, 2017)

전자빔 표면조사에 따른 SnO2 박막의 전기적, 광학적 특성 연구

송영환 ( Young-hwan Song ),문현주 ( Hyun-joo Moon ),김대일 ( Daeil Kim ) 한국열처리공학회 2016 熱處理工學會誌 Vol.29 No.3

We have considered the influence of electron irradiation on the optical and electrical properties of SnO2 thin films deposited with reactive RF magnetron sputtering. After deposition, the films electron irradiated at 300 eV shows a lower sheet resistance of 277 Ω/ □ and the optical transmittance in a visible wave length region also influenced with the electron irradiation energy. The film that electron irradiated at 400 eV shows a higher optical transmittance of 82.6% in this study. By comparison of figure of merit, it is concluded that the post deposition electron irradiation at 300 eV is the optimum condition for the enhancement of opto-electrcal performance of SnO2 thin film in this study.