H₂O₂로 유발된 뇌신경세포 상해에 대한 구진의 보호효과

안성훈,구성태,김선영,김경식,손인철,Ahn, Sung-hun,Koo, Sung-tae,Kim, Sun-young,Kim, Kyung-sik,Sohn, In-cheul 대한침구의학회 2004 대한침구의학회지 Vol.20 No.2

Objective : This study was produced to examine the effects of moxibustion that had been played important role to traditional oriental medical treatment on disease. Recently, it was reported that moxi-tar which is generated in the process of moxibustion as burning combustibles decreased NO and iNOS generation in C6-glioma and RAW 264.7 cells in our lab. Methods : C6-glioma cells were cultured in RPMI 1640 with FBS 10% in CO2 incubator. To study the protective effects of moxi-tar, we observed cell viability, DPPH activity, SOD activity, catalase activity and cell morphology after injury with $H_2O_2$. Results and Conclusions : Moxi-tar increased cell viability about twice as much as that of being injury by $H_2O_2$(moxi-tar $40{\mu}g/m{\ell}$, $H_2O_2$ $500{\mu}M$). And the results of free radical scavenger activity($80{\mu}g/m{\ell}$ : $78.91{\pm}4.4%$), SOD activity and catalase activity($80{\mu}g/m{\ell}$ : 21.6unit/mg protein) were increased by moxi-tar as dose-dependent manner. So we concluded that the effects of moxibustion which is played important role in Oriental medicine, might be free radical scavenger effects induced by moxi-tar. Conclusion : These results indicate that tBHP induces apoptosis through a lipid peroxidation-dependent mechanism and JS exerts the protective effect against the apoptosis by preventing peroxidation of membrane lipids.

원락(原絡) 배혈(配穴) 전침 자극이 백서의 족과 염좌(捻挫) 통증에 미치는 영향(影響)

김선영,구성태,김경식,손인철,Kim, Sun-Young,Koo, Sung-Tae,Kim, Kyoung-Sik,Sohn, In-Cheul 경락경혈학회 2005 Korean Journal of Acupuncture Vol.22 No.1

Objectives : In the present study, the effect of electroacupuncture (EA) applied to SI6 and won-rak point on the ankle sprain model was examined. A common source of persistent pain in humans is the lateral ankle sprain. Methods : To model this condition, the rat's right ankle was bent repeatedly, overextending lateral ligaments, for 4 min under enflurane anesthesia. The rat subsequently showed swelling of the ankle and a reduced stepping force of the affected limb for the next several days. The reduced stepping force of the limb was presumably due to a painful ankle. EA was applied to the several acupuncture point on the contralateral forelimb for 30 min under gaseous anesthesia. After the termination of EA, behavioral tests measuring stepping force and Paw volume were Periodically conducted during the next 4 h and 18 h respectively. Results : EA applied to $SI_6$ with won-rak point produced more powerful improvement of stepping force of the sprained foot than to $SI_6$ alone lasting for at least 4 h. However, neigher $KI_4$ point nor $BL_{64}$ point produced any significant increase of weight bearing force. The improvement of stepping pressure was interpreted as an analgesic effect. The analgesic effect was specific to the acupuncture point since the analgesic effort on the ankle sprain pain model could not be mimicked by EA applied to $KI_4$ or $BL_{64}$. In addition, EA applied to $SI_6$ with won-rak combination point showed inhibitory effect on the paw edema induced by ankle sprain. Also, COX-2 protein expression increased by ankle sprain were suppressed by the EA stimulation. Conclusion : These data suggest that EA with won-rak combination point produces a more potent analgesic effect on the ankle sprain pain model in the rat and that EA with won-rak combination point induced anti-inflammatory effect through the suppression of COX-2 protein expression.

족삼리(足三里) (ST36) 자침(刺鍼)이 복부(腹部) 한열변화(寒熱變化)에 미치는 영향(影響)

심원보,김재효,김종성,송재수,김경식,손인철,Sim Won-Bo,Kim Jae-Hyo,Kim Jong-Sung,Song Jae-Soo,Kim Kyung-Sik,Sohn In-Cheul 경락경혈학회 2003 대한침구의학회지 Vol.21 No.2

This study was examined for effects of acupuncture of Zusanli (ST36) on the abdominal thermography of health subject. The volunteers who participating in this study had taken rest for 20-30 mins in room temperature (23-$25^{\circ}C$) before the examination and informed them what to prohibit smoking, drinking and administration of drug for the previous day. The thermography of abdomen including a below part of the chest was taken using Infra-Red Imaging System (IR 2000, MEDI-CORE Co, Korea) by time interval of 15 minutes at 15 min before, just before and 15 min after, 30 min after and 45 min after acupuncture stimulation. Acupuncture was applied to the left ST36 acupoint for 30 mins. The results showed that acupuncture of ST36 significantly had more potencies of changes on all the of abdominal thermography than those of control group. Also, it was observed that the quantities of thermal changes following acupuncture of ST36 been increased significantly comparing that of control group at all the ROIs (region of interest). Observed the thermography classified by ROI, however, it was failed that acupuncture of ST36 could modulate the specific areas concerning to the abdominal pathway of Stomach Meridian. These results suggest that acupuncture of ST36 may modulate thermal distributions and changes of abdominal areas including the below of chest.

어제(魚際)(LU10) 자침(刺鍼)이 복부(腹部) 한열변화(寒熱變化)에 미치는 영향(影響)

전문기,김재효,변기원,도진우,김경식,손인철,Jeon Moon-Kee,Kim Jae-Hyo,Byun Kee-Won,Do Jin-Woo,Kim Kyung-Sik,Sohn In-Cheul 경락경혈학회 2003 대한침구의학회지 Vol.21 No.2

This study was examined for characteristics of acupuncture of LU10 on the abdominal thermography of health subject. The volunteers who participating in this study had taken rest for 20 - 30 mins in room temperature $(23-25^{\circ}C)$ before the examination and informed them what to prohibit smoking, drinking and administration of drug for the previous day. The thermography of abdomen including a below part of the chest was taken using Infra-Red Imaging System (IR 2000, MEDI-CORE Co., Korea) by time interval of 15 minutes at 15 min before, just before and 15 min after, 30 min after and 45 min after acupuncture stimulation. Acupuncture was applied to both LU10 for 30 mins. The results showed that acupuncture of LU10 for 30 mins had more potencies of changes on all the ROIs of abdominal thermography than those of control group. Also, those responses were significantly clear at the A1, A3, A6, A7 and A9 areas. It was observed that the quantities of thermal changes following acupuncture of LU10 been increased significantly comparing that of control group at the all ROIs (region of interest). These effects have more potencies at the upper (A1 and A2) and below (A7, A8, A9) abdominal areas than midline ones (A4, A5, and A6). These results suggest that acupuncture of LU10 having the characteristics of controls of chill and fever (寒熱) may modulate thermal distributions and changes of abdominal areas including the below of chest.

CFA로 유도한 관절염에서 선혈(選穴) 따른 전침(電鍼)의 진통효과 차이

양범식 ( Bum Sik Yang ),구성태 ( Sung Tae Koo ),김경식 ( Kyoung Sik Kim ),손인철 ( In Cheul Sohn ) 대한경락경혈학회 2005 Korean Journal of Acupuncture Vol.22 No.1

Objective: In the present study, the effect of electroacupuncture (EA) applied to several acupoints and non-acupoint in CFA-induced knee arthritis was examined. Methods: A common source of persistent pain in humans is arthritis. Arthritis was induced by injection of CFA 125 ㎕ into knee joint cavity under enflurane anesthesia. The rat subsequently showed swelling of the ankle and a reduced stepping force of the affected limb for the next several days. The reduced stepping force of the limb was presumably due to a painful knee. EA was applied to either of LR2, LI4, or non-acupoint on the contralateral forelimb for 30 min under gaseous anesthesia. After the termination of EA, behavioral tests measuring stepping force were periodically conducted during the next 4 h. Results: EA applied to LR2 point produced a significant improvement of stepping force of the affected foot lasting for at least 2 h. However, neigher LI4 point nor non-point produced any significant increase of weight bearing force. The improvement of stepping pressure was interpreted as an analgesic effect. The analgesic effect was specific to the acupuncture point since the analgesic effect on CFA-induced knee arthritic pain model could not be mimicked by EA applied to a point, LI4 or non-acupoint. In addition, both NO production and iNOS protein expression increased by arthritis were suppressed by EA applied to LR2 point. Conclusion: These data suggest that EA produces a potent analgesic effect in the rat model of CFA-induced knee arthritis. This analgesic effect is produced by applying EA to an acupoint at opposite side from the painful area in a stimulus point-specific way.

원락(原絡) 배혈(配穴) 전침 자극이 백서의 족과(足과) 념좌(捻挫) 통증에 미치는 영향(影響)

김선영 ( Sun Young Kim ),구성태 ( Sung Tae Koo ),김경식 ( Kyoung Sik Kim ),손인철 ( In Cheul Sohn ) 대한경락경혈학회 2005 Korean Journal of Acupuncture Vol.22 No.1

Objectives: In the present study, the effect of electroacupuncture (EA) applied to SI6 and won-rak point on the ankle sprain model was examined. A common source of persistent path in humans is the lateral ankle sprain. Methods: To model this condition, the rat`s right ankle was bent repeatedly, overextending lateral ligaments, for 4 mm under enflurane anesthesia. The rat subsequently showed swelling of the ankle and a reduced stepping force of the affected limb for the next several days. The reduced stepping force of the limb was presumably due to a painful ankle. EA was applied to the several acupuncture point on the contralateral forelimb for 30 min under gaseous anesthesia. After the termination of EA, behavioral tests measuring stepping force and paw volume were periodically conducted during the next 4 h and 18 h respectively. Results: EA applied to SI6 with won-rak point produced more powerful improvement of stepping force of the sprained foot than to SI6 alone lasting for at least 4 h. However, neigher KI4 point nor BL64 point produced any significant increase of weight bearing force. The improvement of stepping pressure was interpreted as an analgesic effect. The analgesic effect was specific to the acupuncture point since the analgesic effect on the ankle sprain pain model could not be mimicked by EA applied to KI4 or BL64. In addition, EA applied to SI6 with won-rak combination point showed inibitory effect on the paw edema induced by ankle sprain. Also, COX-2 protein expression increased by ankle sprain were suppressed by the EA stimulation. Conclusion: These data suggest that EA with won-rak combination point produces a more potent analgesic effect on the ankle sprain pain model in the rat and that EA with won-rak combination point induced anti-inflammatory effect through the suppression of COX-2 protein expression.

일과성(一過性) 전뇌(前腦) 허혈(虛血) 손상(損傷)에 대한 환도(環跳) (GB30) 전침자극(電鍼刺戟)의 효과(效果)

김풍식 ( Poong Sik Kim ),김재효 ( Jae Hyo Kim ),최동옥 ( Dong Ok Choi ),김경식 ( Kyung Sik Kim ),손인철 ( In Cheul Sohn ) 대한경락경혈학회 2002 Korean Journal of Acupuncture Vol.19 No.1

Acupuncture and herbal medicine have been used to prevent and treat the cerebrovascular accident, such as a stroke, and many studies of acupuncture and moxibustion concerning to the stroke have been undertaken in the human and various animals. However, it was not published the protective effect of the electroacupuncture (EA) of Huan-do (GB30) on the transient forebrain ischemia injury. The neuroprotective effects of EA (2 ms, 10 Hz, and 1-2 mA) of GB30 on the transient forebrain ischemia injury was investigated by western blot of nNOS and hematoxylin and eosin stain in Sprague-Dawley rats. The transient forebrain ischemia injury resulted in increased expression of nNOS in the brain for 6 hrs after ischemia, and EA decreased significantly expression of nNOS protein in brain increased by transient forebrain ischemia injury. The survived neuronal cell stained with hematoxylin and eosin (H&E) decreased in the hippocampus by the 7 days after ischemia comparing with the normal. Comparing with the normal, the survived neurons seriously decreased cell in the hippocampus after the injury. However, the proportion of survived neurons was increased in EA treatment. These results suggest that EA of GB30 have neuroprotective effects on transient forebrain ischemia injury.

족삼리(足三里) (ST36) 자침(刺鍼)이 안면부(顔面部) 한열변화(寒熱變化)에 미치는 영향(影響)

김영태 ( Yong Tae Kim ),김재효 ( Jae Hyo Kim ),황재호 ( Jae Ho Hwang ),김경식 ( Kyung Sik Kim ),손인철 ( In Cheul Sohn ) 대한경락경혈학회 2002 Korean Journal of Acupuncture Vol.19 No.2

This study was examined for effects of acupuncture of Zusanli (ST36) on the facial thermography in health subjects. The volunteers who participating in this study had taken rest for 20-30 mins in room temperature (23-25℃) before the examination and informed them what to prohibit smoking, drinking and administration of drug for the previous day. The thermography of face was taken using Infra-Red Imaging System (IR 2000, MEDI-CORE Co., Korea) by time interval of 15 minutes at 15 min before, just before and 15 min after, 30 min after and 45 min after acupuncture stimulation. Acupuncture was applied to the left ST36 for 30 mins. The results showed that acupuncture of ST36 significantly decreased the temperature of all the areas of facial surface comparing to those of control group. Also, it was observed that the quantities of thermal changes following acupuncture of ST36 been increased significantly at the A1, A4, A6, A7 and A9 ROIs (region of interest) comparing that of control group. Observed the thermography classified by ROI, it was clear the fact that acupuncture of ST36 could modulate the specific areas concerning to the facial pathway of Stomach Meridian, because the thermal responses following acupuncture of ST36 were specific at the A1, A2, A5 and A9 ROIs, relatively. These results suggest that acupuncture of ST36 may modulate thermal distributions and changes of facial areas concerned with Stomach Meridian.

자침시(刺鍼時) 탄침(彈鍼)의 수기자극(手技刺戟)이 전위변화(電位變化)에 미치는 영향(影響)

김용득 ( Yong Deug Kim ),김영태 ( Young Tae Kim ),박성섭 ( Sung Sub Park ),박귀종 ( Gwi Jong Park ),손인철 ( In Cheul Sohn ),김경식 ( Kyung Sik Kim ) 대한경락경혈학회 2004 Korean Journal of Acupuncture Vol.21 No.4

Objectives: To understanding biological mechanism of acupuncture therapy, we have proposed a hypothesis. First of all, there exists electric property in meridian and meridian point. Second of all, energy flowing in meridian is related with electric property. Third of all, there is electronic interaction between the operator who performs acupuncture therapy and the receiver who is given acupuncture therapy. Forth of all, acupuncture effects may depend on the electric capacity which is transferred between the operator and the receiver via acupuncture needle. Methods: Under the hypothesis, we studied the effects of electric charge in ST37(+) and ST39(-) generated by Tanchim (彈鍼) manipulation which was stimulated at ST36 point. And compared with data on the changes of electric charge from the hand of the operator during acupuncture stimulation. Electric charge induced via acupuncture needle from the operator may be important factor that causes the changes of electric charge in meridian and acupoint in the receiver. Therefore we investigated the changes of electric charge induced by the operator using Maclab 400 by the following methods. The one was in stable electric circle condition and the other was in unstable electric circle condition. Results: In this experiments, the changes of electric charge from the stimulation type of Tanchim manipulation performed in our lab condition in acupuncture therapy was induced at least three factor, one was the difference of bio-potentials between the operator and the receiver of acupuncture therapy, another was the depth of acupuncture insertion from the skin of the receiver, the otter was an electromyogram of the receiver. Conclusion: The data imply that the first factor should make a capacitance current when the operator touched the acupuncture needle which was inserted in the receiver. Therefore, the results suggest that capacitance currents stimulus in electronic view may be important factor in acupuncture therapy between the operator and the receiver of acupuncture therapy.

구진의 H2O2로 유발된 뇌신경세포 상해에 대한 보호 기전 연구

안성훈 ( Seoung Hoon Ahn ),안영남 ( Young Nam Ahn ),백대봉 ( Dae Bong Baek ),송문영 ( Moon Young Song ),김경식 ( Kyoung Sik Kim ),손인철 ( In Cheul Sohn ) 대한경락경혈학회 2005 Korean Journal of Acupuncture Vol.22 No.2

Objective: This study was produced to examine the effects of moxibustion that had been played important role to traditional oriental medical treatment on disease. Recently, it was reported that moxi-tar which is generated in the process of moxibustion as burning combustibles decreased NO and iNOS generation in C6-glioma and RAW 264.7 cells in our Lab. The purpose of this research was to investigate the protect reaction on cell injury induced by the H2O2 in C6-glioma cells. Methods: C6-glioma cells were cultured in RPMI 1640 with FBS 10% in CO2 incubator. To study the protective effects of moxi-tar, we observed cell viability, DPPH activity, SOD activity, catalase activity and cell morphology after injury with H202. Results: Moxi-tar increased cell viability about twice as much as that of being injury by H2O2 (moxi-tar 40 μg/ml, H2O2 500 μM). And the results of free radical scavenger activity (80 μg/ml: 78.91±4.4%), SOD activity and catalase activity (80 μg/ml: 21.6 unit/mg protein) were increased by moxi-tar as dose-dependent manner. Conclusion: we concluded that the effects of moxibustion which is played important role in Oriental medicine, might be free radical scavenger effects induced by moxi-tar.