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잔류 항균물질에 대한 미생물학적 간이검사법의 검출감도 비교
정승희(Sung Hee Jung),김진우(Jin Woo Kim),손상규(Sang Gyu Son) 한국수산과학회 1999 한국수산과학회지 Vol.32 No.3
Minimun-detectable levels to 28 antibacterial agents used for the prevention and the treatment of fish diseases were determined to establish optimal detective method of bioassay in fish by the EEC 4-plate method, the modified method of EEC 4-plate and the standard method of analysis in food safety regulation. The test organisms used in the methods of bioassay were as follows: Bacillus subtilis BGA(B. subtilis)and Micrococcus luteus ATCC 9341(M. luteus) in the EEC 4-plate method, B. subtilis, M. luteus, and Bacillus cereus var. mycoides ATCC 11778(B. cereus) in the EEC 4-plate and B. subtilis, M. luteus, B. cereus and Bacillus stearothermophilis var. calidolactis C-953 (B. starothermophilis)in the standard method. The standard method showed predominant sensitivity in the detection of penicillins(PCs), and was also highly sensitive to aminoglycosides (AGs). The sensitivity of standard method in the detection of tetracyclines (TCs), macrolides(MLs), nitrofuran derivatives(NFs) and quinolones(Qns) was very low, and against sulfonamides (SAs), however, was extremely low. The modified method of EEC 4-plate showed very high sensitivity to TCs. Both the EEC 4-plate and the modified method of EEC 4-plate showed competitively high sensitivity in the detection of PCs, MLs, NFs, QNs and SAs. All the methods studied in the experiment showed very low sensitivity against chloramphenicol (CMs). Consequently, the modified method of EEC 4-plate was the best bioassay method with a wide .range of sensitivity for the optimal detection of the residual antibacterial agents in fish.
갯장어 Muraenesox cinereus의 난소 발달에 따른 혈중 Estradiol-17β 변동
김대중(Dae-Jung Kim),김이청(Yi-Cheong Kim),손맹현(Maeng-Hyun Son),이정의(Jung-Uie Lee),손상규(Sang-Gyu Son),한창희(Chang-Hee Han) 한국생명과학회 2009 생명과학회지 Vol.19 No.12
본 연구는 통영 해역에서 채집되어진 갯장어, Muraenesox cinereus의 생식년주기에 있어서 난소 발달과 생식소중량지수 (gonadosomatic index; GSI) 변화에 따른 혈중 estradiol- 17β (E₂) 농도 변화를 서로 연관시켜 조사하였다. 난소 성숙도는 조직학적 관찰을 기초로 주변인기(11월-2월), 유구기(3월-4월), 초기 난황형성기(4월-5월) 및 후기 난황형성기(6월-10월)로 나 눌 수 있었다. GSI의 계절적 변화는 수온과 밀접한 연관성을 가지고 있으며, 난소의 성숙 정도에 영향을 미쳤다. 혈중 E2 농도는 GSI 변화와 관련이 있어 4월에 증가하여 7월에 년중 최고치에 도달하였고 10월까지 비교적 높은 농도로 유지하였다. 이러한 결과들은 GSI와 혈중 E2 농도 변화는 갯장어 난소 발달과 밀접한 관련이 있음을 나타내고 있다. 그러나 본 연구에서는 산란기의 암컷 갯장어를 채집하지 못하였다. 이러한 이유로 산란기의 개체는 외해의 심해쪽으로 이동하기 때문이라고 추측된다. This study correlated changes in the plasma levels of estradiol-17β (E2) with changes in the gonadosomatic index (GSI) and ovarian development during the annual reproductive cycle of the pike eel Muraenesox cinereus, collected at the Tongyung coast region. Ovarian maturity was classified based on histological observations; the perinucleolus stage (November to February), the oil droplet stage (March to April), the early vitellogenic stage (April to May) and the late vitellogenic stage (June to October). Seasonal changes in the GSI were correlated with water temperature and reflected the degree of ovarian maturity. Plasma E2 levels were correlated with changes in the GSI, which increased from April to a peak in July, and the levels remained comparatively high until October. These data indicated that changes in the GSI and plasma E2 levels are correlated with the annual ovarian activity of the pike eel. In this study, however, female pike eels were not collected during the spawning stage. Therefore, spawning of this species seemed to be closely related to its migration toward the deep sea of offshore.